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The map label for this gene is dapE [H]

Identifier: 46446923

GI number: 46446923

Start: 1544115

End: 1545557

Strand: Direct

Name: dapE [H]

Synonym: pc1289

Alternate gene names: 46446923

Gene position: 1544115-1545557 (Clockwise)

Preceding gene: 46446920

Following gene: 46446928

Centisome position: 63.95

GC content: 39.85

Gene sequence:

>1443_bases
ATGGGTCCTTCTTTACCAAGCTGTCTAGCAGAAATTAAATACTTGATCGAGCAAAATCGAGAAGAATGGCTAAAAGAGTA
TTATACTTTTTTAAGTTTTCCCAGTATTAGTTCTGAAACTCACTTTCAAGTCTCTCTATTAAACTGTGCAAACTGGGTTG
TCGATTATTTAAAAACACTAGGTTTTGAGGTAGAGCTTTGGCCGACTGAACAAGATGGCCCCCCAGTTATTTACGCCACA
CACTTAAAGGCAGGTGCCGACAAACCCACCCTTCTCATTTATAATCACTACGATGTACAACCTGCCGATCCCCTAAATGA
ATGGAAAACAGATCCTTTTCAACCAAGCTTAAGAGACGGAAGTGTTTATGCTCGAGGAGCCCAAGATAATAAAGGACAGT
GTTTTTATGTTCTTCAAGCCTTAAAATTTTATTTAAAACAATATTCTCGATTACCGATTAACATCAAACTTTGTATTGAG
GGAGAAGAGGAAATTGGTAGTGCAGGATTATCTCGATTATTACCAACCAAAGCAGAAGCTTTAAAGGCTGATTATTTAGC
CGTTGTCGATTTAGGACTTAGGGACTCTCAAGTCCCTGCCGTGACATTAGGCATTCGGGGTATTCTCACAATGGATGTCG
AAGTACAAGGTTCAGATAATGATTTACATTCTGGCTCCCATGGGGGAATCGCCATCAATCCTATTCATGCGCTTGTAGCC
ATGCTCGCTTCATTAAGAGATGCTTCAGGAAAAATTACTGTTAATGGATTTTATGATGATGTTGAAGAGATGAGCTTGGA
AGAGCGTTCATCGGTTTCTTTTTATTTTGATTTAGCTGACTATCAAAAGCTAACAGGGGCCTATCCTGGTGGAGGAGAAA
AAGATAAAACAGCTTTAGAAAGAGCCTGGGCAAGACCTACTTTAGAAATTAATGGGATTAATGGCGGATATACAGGCAAA
GGATTTAAAACAGTGATTCCTGCGAAAGCCTCAGCTAAAATATCTTGCCGATTAGTTTCTCAACAAGATCCAAAAAAAAT
TGGAAGACTCATCGAGCACCATCTTAACGAAGCCGCTCCCCAAGGGATTCAAGTAAGAATTACTATACATCAAGGGCAAG
GAAGGGCCATTCGAGTTAGTCCAAAATCGCAACTGGTCGCATCGTTTTCAGAGGCTTTTCAGGAAGTTTTTGGTGTGCCT
TGTGAATTCATTTTTGAAGGAGCCTCAATCCCTATTGTTCCTGAACTTGGAATGGCTTGTGGAGGAGAAGTTATTTTAAT
TGGATTGGGACTCACAACAGATCTTATTCATGCTCCGAATGAACATTTTGGGTTGGATCGGTTAGAAAAAGGGATCCTGA
TCATAGCTAGAGCGATTGAATTATTAGCGCAACATCCCAAAGACAAACGCTTAGAAAAATGCTCTAAGAGTTTGTCTTTA
TAA

Upstream 100 bases:

>100_bases
CGTTTATGGTTCTTCTCTTGGAAAACTACAGATTATTTTTTCTATTTTACTTAATTGATTGCGGAGCCTACTATGTGAGG
CAAATTTAAAAAGGGGAAAT

Downstream 100 bases:

>100_bases
ATCTAACCAAAAAAATTAGGCGGCTTGATCTACTACAAATTGGTATTTACTAAGAGAATTGAGATCTTCTTCAATTTCAC
GCCCCATGTTTGCTTCTAAC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MGPSLPSCLAEIKYLIEQNREEWLKEYYTFLSFPSISSETHFQVSLLNCANWVVDYLKTLGFEVELWPTEQDGPPVIYAT
HLKAGADKPTLLIYNHYDVQPADPLNEWKTDPFQPSLRDGSVYARGAQDNKGQCFYVLQALKFYLKQYSRLPINIKLCIE
GEEEIGSAGLSRLLPTKAEALKADYLAVVDLGLRDSQVPAVTLGIRGILTMDVEVQGSDNDLHSGSHGGIAINPIHALVA
MLASLRDASGKITVNGFYDDVEEMSLEERSSVSFYFDLADYQKLTGAYPGGGEKDKTALERAWARPTLEINGINGGYTGK
GFKTVIPAKASAKISCRLVSQQDPKKIGRLIEHHLNEAAPQGIQVRITIHQGQGRAIRVSPKSQLVASFSEAFQEVFGVP
CEFIFEGASIPIVPELGMACGGEVILIGLGLTTDLIHAPNEHFGLDRLEKGILIIARAIELLAQHPKDKRLEKCSKSLSL

Sequences:

>Translated_480_residues
MGPSLPSCLAEIKYLIEQNREEWLKEYYTFLSFPSISSETHFQVSLLNCANWVVDYLKTLGFEVELWPTEQDGPPVIYAT
HLKAGADKPTLLIYNHYDVQPADPLNEWKTDPFQPSLRDGSVYARGAQDNKGQCFYVLQALKFYLKQYSRLPINIKLCIE
GEEEIGSAGLSRLLPTKAEALKADYLAVVDLGLRDSQVPAVTLGIRGILTMDVEVQGSDNDLHSGSHGGIAINPIHALVA
MLASLRDASGKITVNGFYDDVEEMSLEERSSVSFYFDLADYQKLTGAYPGGGEKDKTALERAWARPTLEINGINGGYTGK
GFKTVIPAKASAKISCRLVSQQDPKKIGRLIEHHLNEAAPQGIQVRITIHQGQGRAIRVSPKSQLVASFSEAFQEVFGVP
CEFIFEGASIPIVPELGMACGGEVILIGLGLTTDLIHAPNEHFGLDRLEKGILIIARAIELLAQHPKDKRLEKCSKSLSL
>Mature_479_residues
GPSLPSCLAEIKYLIEQNREEWLKEYYTFLSFPSISSETHFQVSLLNCANWVVDYLKTLGFEVELWPTEQDGPPVIYATH
LKAGADKPTLLIYNHYDVQPADPLNEWKTDPFQPSLRDGSVYARGAQDNKGQCFYVLQALKFYLKQYSRLPINIKLCIEG
EEEIGSAGLSRLLPTKAEALKADYLAVVDLGLRDSQVPAVTLGIRGILTMDVEVQGSDNDLHSGSHGGIAINPIHALVAM
LASLRDASGKITVNGFYDDVEEMSLEERSSVSFYFDLADYQKLTGAYPGGGEKDKTALERAWARPTLEINGINGGYTGKG
FKTVIPAKASAKISCRLVSQQDPKKIGRLIEHHLNEAAPQGIQVRITIHQGQGRAIRVSPKSQLVASFSEAFQEVFGVPC
EFIFEGASIPIVPELGMACGGEVILIGLGLTTDLIHAPNEHFGLDRLEKGILIIARAIELLAQHPKDKRLEKCSKSLSL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=479, Percent_Identity=31.9415448851775, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI271398239, Length=471, Percent_Identity=29.9363057324841, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI271398277, Length=319, Percent_Identity=30.7210031347962, Blast_Score=145, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17563146, Length=460, Percent_Identity=32.8260869565217, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI193211059, Length=463, Percent_Identity=28.2937365010799, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321159, Length=489, Percent_Identity=26.9938650306748, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319758, Length=462, Percent_Identity=25.3246753246753, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24585880, Length=460, Percent_Identity=32.1739130434783, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 52770; Mature: 52639

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSLPSCLAEIKYLIEQNREEWLKEYYTFLSFPSISSETHFQVSLLNCANWVVDYLKTL
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
GFEVELWPTEQDGPPVIYATHLKAGADKPTLLIYNHYDVQPADPLNEWKTDPFQPSLRDG
CCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC
SVYARGAQDNKGQCFYVLQALKFYLKQYSRLPINIKLCIEGEEEIGSAGLSRLLPTKAEA
CEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHH
LKADYLAVVDLGLRDSQVPAVTLGIRGILTMDVEVQGSDNDLHSGSHGGIAINPIHALVA
HHHCEEEEEECCCCCCCCCEEEEEECEEEEEEEEEECCCCCCCCCCCCCEEECHHHHHHH
MLASLRDASGKITVNGFYDDVEEMSLEERSSVSFYFDLADYQKLTGAYPGGGEKDKTALE
HHHHHCCCCCEEEEECCHHCHHHHCHHHCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHH
RAWARPTLEINGINGGYTGKGFKTVIPAKASAKISCRLVSQQDPKKIGRLIEHHLNEAAP
HHHCCCCEEEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
QGIQVRITIHQGQGRAIRVSPKSQLVASFSEAFQEVFGVPCEFIFEGASIPIVPELGMAC
CCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCC
GGEVILIGLGLTTDLIHAPNEHFGLDRLEKGILIIARAIELLAQHPKDKRLEKCSKSLSL
CCCEEEEEECCHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GPSLPSCLAEIKYLIEQNREEWLKEYYTFLSFPSISSETHFQVSLLNCANWVVDYLKTL
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
GFEVELWPTEQDGPPVIYATHLKAGADKPTLLIYNHYDVQPADPLNEWKTDPFQPSLRDG
CCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC
SVYARGAQDNKGQCFYVLQALKFYLKQYSRLPINIKLCIEGEEEIGSAGLSRLLPTKAEA
CEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHH
LKADYLAVVDLGLRDSQVPAVTLGIRGILTMDVEVQGSDNDLHSGSHGGIAINPIHALVA
HHHCEEEEEECCCCCCCCCEEEEEECEEEEEEEEEECCCCCCCCCCCCCEEECHHHHHHH
MLASLRDASGKITVNGFYDDVEEMSLEERSSVSFYFDLADYQKLTGAYPGGGEKDKTALE
HHHHHCCCCCEEEEECCHHCHHHHCHHHCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHH
RAWARPTLEINGINGGYTGKGFKTVIPAKASAKISCRLVSQQDPKKIGRLIEHHLNEAAP
HHHCCCCEEEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
QGIQVRITIHQGQGRAIRVSPKSQLVASFSEAFQEVFGVPCEFIFEGASIPIVPELGMAC
CCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCC
GGEVILIGLGLTTDLIHAPNEHFGLDRLEKGILIIARAIELLAQHPKDKRLEKCSKSLSL
CCCEEEEEECCHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA