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The map label for this gene is cysS

Identifier: 46446869

GI number: 46446869

Start: 1469182

End: 1470618

Strand: Reverse

Name: cysS

Synonym: pc1235

Alternate gene names: 46446869

Gene position: 1470618-1469182 (Counterclockwise)

Preceding gene: 46446870

Following gene: 46446868

Centisome position: 60.91

GC content: 35.56

Gene sequence:

>1437_bases
ATGAATGAACATGCCCTAAAAAATATTCCCCTCAGGCTTTATAATACGGCTGAGCGTCAAAAGCAAGAGCTTAAACCAAT
AAAAGGCAACCATATACAACTCTACACATGTGGACCAACTGTTTATCACTATGCACACATTGGAAATTTTCGAACTTACA
TCTTTGAAGATTTACTAAGAAGAACAATTCAATTTTTTGGGTTTTCTATTACACAGGTTATGAATCTTACTGATGTGGAT
GACAAAACAATTCGAGGAGCTATTGCTAAGGGTATTACTCTTGATGAGTATACGAAGCCTTATAAGGATGCTTTTTTTGA
AGATTTAAAAACTTTAAATATCCAATCTGCAGAGTATTATCCAGCTGCTACTGATTATATTCCAGCTATGATTGAGATGA
TTAAAGTTCTTTTAGATAAAAAAGTCGCTTATAAAGGAGGGGATGGGAGTATTTATTATGCCATTAACCAATTCCCTCGC
TATGGTTGTTTATCTCACTTGCATTTAGAAGACTTACAAGCAGGGGCCTCTGAACGAGTGGCTGCTGATGAATACGAAAA
AGAACATGTCGCAGACTTTGTGTTGTGGAAAAGTTATGATTCTGAGCGTGATGGTCAAATTTATTGGGAGAGTCCATTTG
GTTTAGGTCGCCCGGGTTGGCATTTAGAATGTTCAGCAATGGCCATGCACCTACTTGGAGAAACGATAGATATTCATGTG
GGAGGAATAGACAATATGTTTCCTCACCATGAAAATGAAATTGCACAATCTGAAGCCTGCTCCGGTAAAAAATTTGTAAA
TTTATGGATGCATGCAGAACATCTTGTCGTTGATCAAAAAAAGATGTCCAAGAGTTTAGGAAATTTTTACACATTGCGTG
ATTTGTTAAATAAAGGTTTTACAGGTATTCAAGTCCGCTATTTACTTCTTCAAACACATTATAAAACGCAATTAAATTTT
ACATTTCAAGGTGTTGAGTCAGTCAAAAGTTCATTGCAACGCCTTAATGACTTTATTCAAAGAATTTATAATATTCAAAC
ACTTCAATCAGACGGCCAGGTTGACTTACTTGTCAATGATGCTTTAATACGATTTGCCGAAGCTCTCGCAGATGACTTGA
ATATTTCTTCCGCTTTAGCTGCTATTTTTGATTTTGTACGTGAAATCAATTGTTTATGTGATGTCAATCAGGTCAGTCAA
AAAGAAGCCGAGACAGTGATAAATCTTATGAAAAAATTTGATACTATTTTAGGCGTATTGACATTTGATAAAAGAGAAGA
AAGTATTCCAGTAGATCTACAAGAAGCTTTTGCTAAAAGGCAACAGGCGAGACAAGAGAAGAACTGGACACTTTCCGATG
AATTACGTGATTTCATTCATCAAAGAGGCTATTTGATTGAAGACACTCCTCAAGGTACCAGATTAAAAAAACAATAA

Upstream 100 bases:

>100_bases
AGTAAATTTTAGAAAATGCTGTTGAATAGAGATTGGTTTTACAACTAGATAAATTCGCAACTTAAGTTATAAAGACATTA
CTGATCGATAGGATACCTTT

Downstream 100 bases:

>100_bases
GGAAATAAAAATGTCCAATCGCGCAAGAACAAAAGATGAAATGTTTATGGTGCGTCTTTATAAAGAAGCTTTAAAACAAG
AAGTAATGGAAGATCCAATA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNEHALKNIPLRLYNTAERQKQELKPIKGNHIQLYTCGPTVYHYAHIGNFRTYIFEDLLRRTIQFFGFSITQVMNLTDVD
DKTIRGAIAKGITLDEYTKPYKDAFFEDLKTLNIQSAEYYPAATDYIPAMIEMIKVLLDKKVAYKGGDGSIYYAINQFPR
YGCLSHLHLEDLQAGASERVAADEYEKEHVADFVLWKSYDSERDGQIYWESPFGLGRPGWHLECSAMAMHLLGETIDIHV
GGIDNMFPHHENEIAQSEACSGKKFVNLWMHAEHLVVDQKKMSKSLGNFYTLRDLLNKGFTGIQVRYLLLQTHYKTQLNF
TFQGVESVKSSLQRLNDFIQRIYNIQTLQSDGQVDLLVNDALIRFAEALADDLNISSALAAIFDFVREINCLCDVNQVSQ
KEAETVINLMKKFDTILGVLTFDKREESIPVDLQEAFAKRQQARQEKNWTLSDELRDFIHQRGYLIEDTPQGTRLKKQ

Sequences:

>Translated_478_residues
MNEHALKNIPLRLYNTAERQKQELKPIKGNHIQLYTCGPTVYHYAHIGNFRTYIFEDLLRRTIQFFGFSITQVMNLTDVD
DKTIRGAIAKGITLDEYTKPYKDAFFEDLKTLNIQSAEYYPAATDYIPAMIEMIKVLLDKKVAYKGGDGSIYYAINQFPR
YGCLSHLHLEDLQAGASERVAADEYEKEHVADFVLWKSYDSERDGQIYWESPFGLGRPGWHLECSAMAMHLLGETIDIHV
GGIDNMFPHHENEIAQSEACSGKKFVNLWMHAEHLVVDQKKMSKSLGNFYTLRDLLNKGFTGIQVRYLLLQTHYKTQLNF
TFQGVESVKSSLQRLNDFIQRIYNIQTLQSDGQVDLLVNDALIRFAEALADDLNISSALAAIFDFVREINCLCDVNQVSQ
KEAETVINLMKKFDTILGVLTFDKREESIPVDLQEAFAKRQQARQEKNWTLSDELRDFIHQRGYLIEDTPQGTRLKKQ
>Mature_478_residues
MNEHALKNIPLRLYNTAERQKQELKPIKGNHIQLYTCGPTVYHYAHIGNFRTYIFEDLLRRTIQFFGFSITQVMNLTDVD
DKTIRGAIAKGITLDEYTKPYKDAFFEDLKTLNIQSAEYYPAATDYIPAMIEMIKVLLDKKVAYKGGDGSIYYAINQFPR
YGCLSHLHLEDLQAGASERVAADEYEKEHVADFVLWKSYDSERDGQIYWESPFGLGRPGWHLECSAMAMHLLGETIDIHV
GGIDNMFPHHENEIAQSEACSGKKFVNLWMHAEHLVVDQKKMSKSLGNFYTLRDLLNKGFTGIQVRYLLLQTHYKTQLNF
TFQGVESVKSSLQRLNDFIQRIYNIQTLQSDGQVDLLVNDALIRFAEALADDLNISSALAAIFDFVREINCLCDVNQVSQ
KEAETVINLMKKFDTILGVLTFDKREESIPVDLQEAFAKRQQARQEKNWTLSDELRDFIHQRGYLIEDTPQGTRLKKQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=464, Percent_Identity=30.8189655172414, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI21269877, Length=281, Percent_Identity=34.1637010676157, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI303304987, Length=281, Percent_Identity=34.1637010676157, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI10835051, Length=281, Percent_Identity=34.1637010676157, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI62240992, Length=281, Percent_Identity=34.1637010676157, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1786737, Length=478, Percent_Identity=37.0292887029289, Blast_Score=307, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI17509797, Length=263, Percent_Identity=34.2205323193916, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17509795, Length=263, Percent_Identity=34.2205323193916, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324082, Length=370, Percent_Identity=30, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI19922218, Length=507, Percent_Identity=31.163708086785, Blast_Score=235, Evalue=6e-62,
Organism=Drosophila melanogaster, GI19922364, Length=368, Percent_Identity=30.1630434782609, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PARUW (Q6MBU0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008234.1
- ProteinModelPortal:   Q6MBU0
- SMR:   Q6MBU0
- STRING:   Q6MBU0
- GeneID:   2781443
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1235
- NMPDR:   fig|264201.1.peg.1235
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   CPRO264201:PC1235-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55042; Mature: 55042

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHALKNIPLRLYNTAERQKQELKPIKGNHIQLYTCGPTVYHYAHIGNFRTYIFEDLLR
CCCCHHCCCCCCEECHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHH
RTIQFFGFSITQVMNLTDVDDKTIRGAIAKGITLDEYTKPYKDAFFEDLKTLNIQSAEYY
HHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
PAATDYIPAMIEMIKVLLDKKVAYKGGDGSIYYAINQFPRYGCLSHLHLEDLQAGASERV
CCHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEEECCCCCHHHHHHCCHHHHHCCCCCCC
AADEYEKEHVADFVLWKSYDSERDGQIYWESPFGLGRPGWHLECSAMAMHLLGETIDIHV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCEEEEEE
GGIDNMFPHHENEIAQSEACSGKKFVNLWMHAEHLVVDQKKMSKSLGNFYTLRDLLNKGF
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGIQVRYLLLQTHYKTQLNFTFQGVESVKSSLQRLNDFIQRIYNIQTLQSDGQVDLLVND
CHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHH
ALIRFAEALADDLNISSALAAIFDFVREINCLCDVNQVSQKEAETVINLMKKFDTILGVL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFDKREESIPVDLQEAFAKRQQARQEKNWTLSDELRDFIHQRGYLIEDTPQGTRLKKQ
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNEHALKNIPLRLYNTAERQKQELKPIKGNHIQLYTCGPTVYHYAHIGNFRTYIFEDLLR
CCCCHHCCCCCCEECHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHH
RTIQFFGFSITQVMNLTDVDDKTIRGAIAKGITLDEYTKPYKDAFFEDLKTLNIQSAEYY
HHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
PAATDYIPAMIEMIKVLLDKKVAYKGGDGSIYYAINQFPRYGCLSHLHLEDLQAGASERV
CCHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEEECCCCCHHHHHHCCHHHHHCCCCCCC
AADEYEKEHVADFVLWKSYDSERDGQIYWESPFGLGRPGWHLECSAMAMHLLGETIDIHV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCEEEEEE
GGIDNMFPHHENEIAQSEACSGKKFVNLWMHAEHLVVDQKKMSKSLGNFYTLRDLLNKGF
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGIQVRYLLLQTHYKTQLNFTFQGVESVKSSLQRLNDFIQRIYNIQTLQSDGQVDLLVND
CHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHH
ALIRFAEALADDLNISSALAAIFDFVREINCLCDVNQVSQKEAETVINLMKKFDTILGVL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFDKREESIPVDLQEAFAKRQQARQEKNWTLSDELRDFIHQRGYLIEDTPQGTRLKKQ
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA