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The map label for this gene is tyrS

Identifier: 46446803

GI number: 46446803

Start: 1396901

End: 1398178

Strand: Reverse

Name: tyrS

Synonym: pc1169

Alternate gene names: 46446803

Gene position: 1398178-1396901 (Counterclockwise)

Preceding gene: 46446807

Following gene: 46446802

Centisome position: 57.91

GC content: 34.82

Gene sequence:

>1278_bases
ATGACAAATGTAATGGATGTTTTACGAGAGCGCGGGTTTATAGATGCTGTGACAAGCGAAGAAATTCGTCAGCTGACAAA
CCAGCCTATTAAAGTTTATTGTGGATTTGATCCGACTGCCGATAGCTTACATTTAGGAAATTTGGTTGCTATCATGGGTC
TTGCTTGGTTTCAAAGGTTTGGTCATACTCCAGTAGCTATTGTGGGAGGAGCAACTGGAATGATTGGCGATCCATCAGGA
AAAAACGCTGAGAGACAACTATTAGATGAAAAAAAGATTCAACTCAATCTAAAAGGTATAAGTAAAAATCTAGAAGCTAT
CTTAGATTTTAATCACCCAACTGCCCCGGCAATCATTTTGAATAACCTTGATTGGTTTAAAAATTTTACTTTTATTTCTT
TTTTGCGTGATGTAGGTAAATTATTTCGATTGAGTCCTATGCTTGCTAAAGACAGTGTAAAGACACGATTAAATTCTGAA
GAAGGAATGAGTTTTACAGAATTTTGTTACCAGATTTTGCAAGGTTATGACTTTTTACATTTATTCGAAAATTATGGCGT
GACTGTCGAGTTAGGAGGAAGCGATCAATGGGGGAATATTACTGCAGGAACAGATTTAATTCGTAAAGTGCATGCCAAGC
CAGCTTATGGAATTACATTTCCTCTTCTGACTAAAAGTGATGGACAGAAATTTGGTAAATCGGAAAAAGGAGCTGTTTGG
TTATCTCCAGATAAATTATCTTCTTATGAATTTTATCAACATTTGATTCGAGTAGAGGATGCGGACGTCATTAATTTAAT
GCGTATGTTGACTTTCTTAGATATGGGAGAAATTCGCCATTATGAGCAAATGATGAAAGAAGCAGATTATGTTCCGCGAA
CAGCTCAAAAAAGATTGGCCGAGGAAATTACACGTTTAGTGCATGGAGAAGAAGGACTTAAAATAGCAATTAAAGTCACA
GAAGGTGTTGCACCAGGCTCTCAAACTAGCTTAAATGCTGATATATTGGAAAAACTTGCAGCTGATATGCCGAGTTGTGA
AATGAAATTAGAAAATGTTTTAAATAAAAAACTAATTGATTTACTTGTAGAAACAAAGTTACAAACAAGCAAAAGTGAAG
CAAGACGTTTATTGCGAAATGGTGGTGTATACATCAATAATAAAAAAATTGAAGATGAGAATCATATTATTAGCGTTGAA
TGTTTGATTTCTAGTCGATTAATATTATTAGCTGCGGGTAAAAAAAATAAAATGATCATTCGGCTTATGGAAGAATAA

Upstream 100 bases:

>100_bases
GGGATACAAAACTTAGTCATAGACATGGAAAAATAAACGTTAACCATTAATTATTGAACTTTAGATATGGCACTTTCGAC
ACTTAGGCAAGGATTTAAAT

Downstream 100 bases:

>100_bases
AAAAGCGATTTTTTTGTTTAAGAATAATTTAAAGAAGTCGTCAACATTGAATGATAAAAAAAAACTCGTTCAATAATGAT
GATAAAGTATTAAGTGTAAA

Product: tyrosine-tRNA ligase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRFGHTPVAIVGGATGMIGDPSG
KNAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAPAIILNNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSE
EGMSFTEFCYQILQGYDFLHLFENYGVTVELGGSDQWGNITAGTDLIRKVHAKPAYGITFPLLTKSDGQKFGKSEKGAVW
LSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLAEEITRLVHGEEGLKIAIKVT
EGVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLIDLLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVE
CLISSRLILLAAGKKNKMIIRLMEE

Sequences:

>Translated_425_residues
MTNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRFGHTPVAIVGGATGMIGDPSG
KNAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAPAIILNNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSE
EGMSFTEFCYQILQGYDFLHLFENYGVTVELGGSDQWGNITAGTDLIRKVHAKPAYGITFPLLTKSDGQKFGKSEKGAVW
LSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLAEEITRLVHGEEGLKIAIKVT
EGVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLIDLLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVE
CLISSRLILLAAGKKNKMIIRLMEE
>Mature_424_residues
TNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRFGHTPVAIVGGATGMIGDPSGK
NAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAPAIILNNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSEE
GMSFTEFCYQILQGYDFLHLFENYGVTVELGGSDQWGNITAGTDLIRKVHAKPAYGITFPLLTKSDGQKFGKSEKGAVWL
SPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLAEEITRLVHGEEGLKIAIKVTE
GVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLIDLLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVEC
LISSRLILLAAGKKNKMIIRLMEE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=415, Percent_Identity=39.0361445783133, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1787925, Length=423, Percent_Identity=47.5177304964539, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25145802, Length=439, Percent_Identity=34.8519362186788, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25145805, Length=310, Percent_Identity=33.8709677419355, Blast_Score=151, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=452, Percent_Identity=36.5044247787611, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI20130383, Length=438, Percent_Identity=39.9543378995434, Blast_Score=320, Evalue=2e-87,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PARUW (Q6MC06)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008168.1
- ProteinModelPortal:   Q6MC06
- SMR:   Q6MC06
- STRING:   Q6MC06
- GeneID:   2779721
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1169
- NMPDR:   fig|264201.1.peg.1169
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   Q6MC06
- ProtClustDB:   CLSK2762340
- BioCyc:   CPRO264201:PC1169-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47817; Mature: 47686

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 33-33 BINDING 170-170 BINDING 174-174 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRF
CCHHHHHHHHCCCEECCCHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHH
GHTPVAIVGGATGMIGDPSGKNAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAPAIIL
CCCCEEEECCCCCCCCCCCCCCHHHHHHCCHHEEEEEHHHHCCCHHEEECCCCCCCEEEE
NNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSEEGMSFTEFCYQILQGYDFLH
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHH
LFENYGVTVELGGSDQWGNITAGTDLIRKVHAKPAYGITFPLLTKSDGQKFGKSEKGAVW
HHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCEEE
LSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLA
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EEITRLVHGEEGLKIAIKVTEGVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLID
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVECLISSRLILLAAGKKNKMII
HHHHHHHHCCHHHHHHHHHCCCEEEECCEECCCCCEEEEEEEECCCEEEEECCCCCCEEE
RLMEE
EEECC
>Mature Secondary Structure 
TNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRF
CHHHHHHHHCCCEECCCHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHH
GHTPVAIVGGATGMIGDPSGKNAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAPAIIL
CCCCEEEECCCCCCCCCCCCCCHHHHHHCCHHEEEEEHHHHCCCHHEEECCCCCCCEEEE
NNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSEEGMSFTEFCYQILQGYDFLH
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHH
LFENYGVTVELGGSDQWGNITAGTDLIRKVHAKPAYGITFPLLTKSDGQKFGKSEKGAVW
HHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCEEE
LSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLA
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EEITRLVHGEEGLKIAIKVTEGVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLID
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVECLISSRLILLAAGKKNKMII
HHHHHHHHCCHHHHHHHHHCCCEEEECCEECCCCCEEEEEEEECCCEEEEECCCCCCEEE
RLMEE
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA