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The map label for this gene is sucB [H]

Identifier: 46446723

GI number: 46446723

Start: 1303768

End: 1304982

Strand: Reverse

Name: sucB [H]

Synonym: pc1089

Alternate gene names: 46446723

Gene position: 1304982-1303768 (Counterclockwise)

Preceding gene: 46446724

Following gene: 46446722

Centisome position: 54.05

GC content: 35.97

Gene sequence:

>1215_bases
TTGCCTTCACAATCAGGAACATGCAAACATTTTAAAACAGGTCTTCAAAACATGAGAACTGACATTAAAGTCCCCTCTAT
GGGCGAGTCGATTACAGAAGTAATGATTGGTCAAATTTTAGTGACTAATGAAACTTTCGTTAAAACTGACGCCGAAATTT
TAGAGCTAGAAACCGATAAGGTTAATCAAGTGTTATTTGCCCCAAAAACGGGAGTAATCACCTTATCTGTACAAACTGGA
GATCGCGTTAAAATTGGAGCTTTGATTGGATTTATTGAGGAAGAGTCTCAAATAGAAAAGAATGTCCCTCAAAAAGAGGA
CAAAGAAATTTCTGCTGAAATTCTTGAACAAAAAGATAGTATAACATCTAGCAAACCTTCTTTTGAAACTGTAGAAAAGG
ATGCGTTGGGAACTTTGCGGCTAACGAAAGAATCTTATCTTTCTGATTTACAGATAGAAGAGCTTTCACCCTCGCAAATA
GCTCAAGATTTGGAGCAAACTGCAAAAACTTTTGAACGTCAAGAAACAAGGCAACCATTGTCTAAAATTCGACAAGTGAT
TGCAAATCGTTTAATAGAAGCTCAGCAAACTATGGCGATGTTGACAACGTTTAATGAAGTAGATTTGTCAGAAATCATTT
CTCTAAGAGAAAAGCATCAAGAGATTTTTATAAAAAAATATGGAATAAAATTAGGCTTTATGTCTTTTTTTGTAAAAGCT
GTTGTCTCTGCTTTAAAAGCTTTTCCCACAGTCAATTCCTACCTCGATCAACAGGACATTGTAGAAAGACATTACTACGA
TATAGGAATTGCAGTTGGAACAGAGCGAGGAACATTTGTGCCTGTGGTCAGACAATGTGATCAACAAAGCTTTGCTCAAA
TTGAATTAGCGATTGATCTTTTTGCTAAAAAAGCAAGAGATGGAAAAATTGCTATGGATGATCTTCAAGGAGGTGGTTTT
ACGATTACGAATGGAGGTGTATATGGCTCTCTTCTTTCCACTCCCATTTTAAATCCACCTCAGTGCGCAATTTTAGGCAT
GCATAAGATTGAAAAAAGGCCGGTTGTCATGGAAGATCAAATTGTGATTCGGCCGATGATGTATCTTGCTTTAAGCTATG
ATCATCGTTTGATTGATGGAAAAGAGTCTGTCGCATTTTTAGTTCATATTAAAAATGCTTTAGAAGATCCTTCTCGCTTA
TTGTTAAATCTTTAA

Upstream 100 bases:

>100_bases
GGAGCTTTATGTTTCCTTATCTTAACGAGTTAATTTCATCTTCTATTCAGCTAAGTTACGTAGGTCGGGAGCGAAGCGCC
ACTCCAGCAACCGGATCTTA

Downstream 100 bases:

>100_bases
AATGTAAGGAAATCACAAATATGGTTGAACACTATGATTTAGCAGTTGTTGGAGCTGGGCCAGGTGGGTATGTGGCAGCT
ATTCGAGCCGCTCAAATGGG

Product: dihydrolipoamide S-succinyltransferase, (2-oxogluturate dehydrogenase complex E2 component), sucB

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPSQSGTCKHFKTGLQNMRTDIKVPSMGESITEVMIGQILVTNETFVKTDAEILELETDKVNQVLFAPKTGVITLSVQTG
DRVKIGALIGFIEEESQIEKNVPQKEDKEISAEILEQKDSITSSKPSFETVEKDALGTLRLTKESYLSDLQIEELSPSQI
AQDLEQTAKTFERQETRQPLSKIRQVIANRLIEAQQTMAMLTTFNEVDLSEIISLREKHQEIFIKKYGIKLGFMSFFVKA
VVSALKAFPTVNSYLDQQDIVERHYYDIGIAVGTERGTFVPVVRQCDQQSFAQIELAIDLFAKKARDGKIAMDDLQGGGF
TITNGGVYGSLLSTPILNPPQCAILGMHKIEKRPVVMEDQIVIRPMMYLALSYDHRLIDGKESVAFLVHIKNALEDPSRL
LLNL

Sequences:

>Translated_404_residues
MPSQSGTCKHFKTGLQNMRTDIKVPSMGESITEVMIGQILVTNETFVKTDAEILELETDKVNQVLFAPKTGVITLSVQTG
DRVKIGALIGFIEEESQIEKNVPQKEDKEISAEILEQKDSITSSKPSFETVEKDALGTLRLTKESYLSDLQIEELSPSQI
AQDLEQTAKTFERQETRQPLSKIRQVIANRLIEAQQTMAMLTTFNEVDLSEIISLREKHQEIFIKKYGIKLGFMSFFVKA
VVSALKAFPTVNSYLDQQDIVERHYYDIGIAVGTERGTFVPVVRQCDQQSFAQIELAIDLFAKKARDGKIAMDDLQGGGF
TITNGGVYGSLLSTPILNPPQCAILGMHKIEKRPVVMEDQIVIRPMMYLALSYDHRLIDGKESVAFLVHIKNALEDPSRL
LLNL
>Mature_403_residues
PSQSGTCKHFKTGLQNMRTDIKVPSMGESITEVMIGQILVTNETFVKTDAEILELETDKVNQVLFAPKTGVITLSVQTGD
RVKIGALIGFIEEESQIEKNVPQKEDKEISAEILEQKDSITSSKPSFETVEKDALGTLRLTKESYLSDLQIEELSPSQIA
QDLEQTAKTFERQETRQPLSKIRQVIANRLIEAQQTMAMLTTFNEVDLSEIISLREKHQEIFIKKYGIKLGFMSFFVKAV
VSALKAFPTVNSYLDQQDIVERHYYDIGIAVGTERGTFVPVVRQCDQQSFAQIELAIDLFAKKARDGKIAMDDLQGGGFT
ITNGGVYGSLLSTPILNPPQCAILGMHKIEKRPVVMEDQIVIRPMMYLALSYDHRLIDGKESVAFLVHIKNALEDPSRLL
LNL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=54.7008547008547, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI203098816, Length=232, Percent_Identity=32.3275862068966, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI203098753, Length=232, Percent_Identity=32.3275862068966, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI31711992, Length=226, Percent_Identity=32.3008849557522, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI110671329, Length=237, Percent_Identity=31.2236286919831, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=30.952380952381, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=41.6666666666667, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1786305, Length=226, Percent_Identity=34.070796460177, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=396, Percent_Identity=40.1515151515151, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17560088, Length=259, Percent_Identity=32.4324324324324, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537937, Length=418, Percent_Identity=23.9234449760766, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=260, Percent_Identity=31.5384615384615, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=39.0547263681592, Blast_Score=276, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6324258, Length=230, Percent_Identity=30, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=52.5862068965517, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=30.1310043668122, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=30.1310043668122, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI18859875, Length=203, Percent_Identity=31.0344827586207, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45246; Mature: 45115

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSQSGTCKHFKTGLQNMRTDIKVPSMGESITEVMIGQILVTNETFVKTDAEILELETDK
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCHHHHCCHHHEEECCCH
VNQVLFAPKTGVITLSVQTGDRVKIGALIGFIEEESQIEKNVPQKEDKEISAEILEQKDS
HCEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
ITSSKPSFETVEKDALGTLRLTKESYLSDLQIEELSPSQIAQDLEQTAKTFERQETRQPL
CCCCCCCHHHHHHHCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SKIRQVIANRLIEAQQTMAMLTTFNEVDLSEIISLREKHQEIFIKKYGIKLGFMSFFVKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVSALKAFPTVNSYLDQQDIVERHYYDIGIAVGTERGTFVPVVRQCDQQSFAQIELAIDL
HHHHHHHCCCHHHHCCHHHHHHHHHHEEEEEEECCCCCEEHHHHHCCHHHHHHHHHHHHH
FAKKARDGKIAMDDLQGGGFTITNGGVYGSLLSTPILNPPQCAILGMHKIEKRPVVMEDQ
HHHHCCCCCEEEEECCCCCEEEECCCEEHHHHHCCCCCCCCCEEECHHHHCCCCCEECCH
IVIRPMMYLALSYDHRLIDGKESVAFLVHIKNALEDPSRLLLNL
HHHHHHHHHHHHCCCCEECCCCCEEEEEEEHHHHCCHHHHHHCC
>Mature Secondary Structure 
PSQSGTCKHFKTGLQNMRTDIKVPSMGESITEVMIGQILVTNETFVKTDAEILELETDK
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCHHHHCCHHHEEECCCH
VNQVLFAPKTGVITLSVQTGDRVKIGALIGFIEEESQIEKNVPQKEDKEISAEILEQKDS
HCEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
ITSSKPSFETVEKDALGTLRLTKESYLSDLQIEELSPSQIAQDLEQTAKTFERQETRQPL
CCCCCCCHHHHHHHCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SKIRQVIANRLIEAQQTMAMLTTFNEVDLSEIISLREKHQEIFIKKYGIKLGFMSFFVKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVSALKAFPTVNSYLDQQDIVERHYYDIGIAVGTERGTFVPVVRQCDQQSFAQIELAIDL
HHHHHHHCCCHHHHCCHHHHHHHHHHEEEEEEECCCCCEEHHHHHCCHHHHHHHHHHHHH
FAKKARDGKIAMDDLQGGGFTITNGGVYGSLLSTPILNPPQCAILGMHKIEKRPVVMEDQ
HHHHCCCCCEEEEECCCCCEEEECCCEEHHHHHCCCCCCCCCEEECHHHHCCCCCEECCH
IVIRPMMYLALSYDHRLIDGKESVAFLVHIKNALEDPSRLLLNL
HHHHHHHHHHHHCCCCEECCCCCEEEEEEEHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]