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The map label for this gene is rlmL [H]

Identifier: 46446591

GI number: 46446591

Start: 1154509

End: 1155510

Strand: Reverse

Name: rlmL [H]

Synonym: pc0957

Alternate gene names: 46446591

Gene position: 1155510-1154509 (Counterclockwise)

Preceding gene: 46446592

Following gene: 46446588

Centisome position: 47.86

GC content: 32.93

Gene sequence:

>1002_bases
ATGAATCCTACTCCTCCATTTATTCTTCCTACCATTGTCGATGGAGAAGATAAAAGCTCACTATTGACGAATCGCATACG
AAAAAATTATCGACACATTCGAAAATGGGCTAAGCGCACTCAAACCAATTGTTTTCGCATTTATGATAAAGAAATTCATC
AATATCCTTTAGCCATTGATTTTTATGATGGTCGATTTTGTGTTCACTATTTTTCTAAAGCAGCCAATGATAATGATTAT
GATCCCCCTAAAGAATTAAAAGAGGCAACGATTCATGCGTTAAAAACAATTTTTAATGTGGAAGAAGAGCTCATTTTTTG
GCGGACCAGATCTAAGCGAAAAGAAACACGCCAATATGAAAGGCAAGGAAATGCTAAAGAATTTTTTTCGATTGTTGAGT
TTGGCGTTCAATTCAAAGTTAATCTTATCGACTATTTAGATACGGGACTTTTTTTAGACCATCGAGAAACACGTAAAATG
GTGACGCAGGCTTCCATTAATAAAAAATTGCTCAATCTTTTTGCTTATACCTGTTCTTTTAGCGTTCATGCAGCTGCTTC
AGGAGCTATTTTTACTAAAAGCGTAGATATGTCTAATACATACACAGAGTGGGGAAAAGATAACTTCCGACTCAACTCTT
TGTCTCTTCAAAATAATGAAGTTATTCGTGCTGATTGTTTAAAATTTTTGGATGACGAAATTAAAAAAAACTTACAATAC
GATGTCATCGTAATTGATCCTCCGACCATTTCTCGATCTAAGAAAATGGATCAACTATTTGATATCCAAGTTGATTATGT
ATCAATGCTTTCTAAAGCCTTAAAATTATTACAAAAAGATGGCGTCATTTTTTTTAGTACGAATTTTCGCAAATTTGTTT
TTAATCAAACTTTATTTCCCTTTTGTTTAATTCAAGATGTTTCTCATAAAACCATCCCTATTGATTTTCATGACTCAAAG
ATACACCGATGTTGGAAAATCATTAAGAAAGCTGATTTCTAA

Upstream 100 bases:

>100_bases
AAAAGAGAAATGTTTGTATACATATGCTGCTAGAAATGGGAAGTATCATCGGCAAATGACACTGACGTTGCCTTTACTAT
AAATTATAATGGAAACCTTA

Downstream 100 bases:

>100_bases
AAAAATTGTTAATATACCAGATATTATTCATGGTTGAAATGGCCAAGCTTTTATTTATTTTGATCAATATCAATTTAAAA
AAGTTAAAAATATGAGGGGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNPTPPFILPTIVDGEDKSSLLTNRIRKNYRHIRKWAKRTQTNCFRIYDKEIHQYPLAIDFYDGRFCVHYFSKAANDNDY
DPPKELKEATIHALKTIFNVEEELIFWRTRSKRKETRQYERQGNAKEFFSIVEFGVQFKVNLIDYLDTGLFLDHRETRKM
VTQASINKKLLNLFAYTCSFSVHAAASGAIFTKSVDMSNTYTEWGKDNFRLNSLSLQNNEVIRADCLKFLDDEIKKNLQY
DVIVIDPPTISRSKKMDQLFDIQVDYVSMLSKALKLLQKDGVIFFSTNFRKFVFNQTLFPFCLIQDVSHKTIPIDFHDSK
IHRCWKIIKKADF

Sequences:

>Translated_333_residues
MNPTPPFILPTIVDGEDKSSLLTNRIRKNYRHIRKWAKRTQTNCFRIYDKEIHQYPLAIDFYDGRFCVHYFSKAANDNDY
DPPKELKEATIHALKTIFNVEEELIFWRTRSKRKETRQYERQGNAKEFFSIVEFGVQFKVNLIDYLDTGLFLDHRETRKM
VTQASINKKLLNLFAYTCSFSVHAAASGAIFTKSVDMSNTYTEWGKDNFRLNSLSLQNNEVIRADCLKFLDDEIKKNLQY
DVIVIDPPTISRSKKMDQLFDIQVDYVSMLSKALKLLQKDGVIFFSTNFRKFVFNQTLFPFCLIQDVSHKTIPIDFHDSK
IHRCWKIIKKADF
>Mature_333_residues
MNPTPPFILPTIVDGEDKSSLLTNRIRKNYRHIRKWAKRTQTNCFRIYDKEIHQYPLAIDFYDGRFCVHYFSKAANDNDY
DPPKELKEATIHALKTIFNVEEELIFWRTRSKRKETRQYERQGNAKEFFSIVEFGVQFKVNLIDYLDTGLFLDHRETRKM
VTQASINKKLLNLFAYTCSFSVHAAASGAIFTKSVDMSNTYTEWGKDNFRLNSLSLQNNEVIRADCLKFLDDEIKKNLQY
DVIVIDPPTISRSKKMDQLFDIQVDYVSMLSKALKLLQKDGVIFFSTNFRKFVFNQTLFPFCLIQDVSHKTIPIDFHDSK
IHRCWKIIKKADF

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=316, Percent_Identity=38.9240506329114, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI145693111, Length=259, Percent_Identity=27.7992277992278, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 39257; Mature: 39257

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTPPFILPTIVDGEDKSSLLTNRIRKNYRHIRKWAKRTQTNCFRIYDKEIHQYPLAID
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHHHCCCEEE
FYDGRFCVHYFSKAANDNDYDPPKELKEATIHALKTIFNVEEELIFWRTRSKRKETRQYE
EECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RQGNAKEFFSIVEFGVQFKVNLIDYLDTGLFLDHRETRKMVTQASINKKLLNLFAYTCSF
HCCCHHHHHHHHHHHHHEEEHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCC
SVHAAASGAIFTKSVDMSNTYTEWGKDNFRLNSLSLQNNEVIRADCLKFLDDEIKKNLQY
EEEECCCCCEEEEECCCCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
DVIVIDPPTISRSKKMDQLFDIQVDYVSMLSKALKLLQKDGVIFFSTNFRKFVFNQTLFP
EEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCHHHH
FCLIQDVSHKTIPIDFHDSKIHRCWKIIKKADF
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPTPPFILPTIVDGEDKSSLLTNRIRKNYRHIRKWAKRTQTNCFRIYDKEIHQYPLAID
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHHHCCCEEE
FYDGRFCVHYFSKAANDNDYDPPKELKEATIHALKTIFNVEEELIFWRTRSKRKETRQYE
EECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RQGNAKEFFSIVEFGVQFKVNLIDYLDTGLFLDHRETRKMVTQASINKKLLNLFAYTCSF
HCCCHHHHHHHHHHHHHEEEHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCC
SVHAAASGAIFTKSVDMSNTYTEWGKDNFRLNSLSLQNNEVIRADCLKFLDDEIKKNLQY
EEEECCCCCEEEEECCCCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
DVIVIDPPTISRSKKMDQLFDIQVDYVSMLSKALKLLQKDGVIFFSTNFRKFVFNQTLFP
EEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCHHHH
FCLIQDVSHKTIPIDFHDSKIHRCWKIIKKADF
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA