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The map label for this gene is def [H]

Identifier: 46446437

GI number: 46446437

Start: 982222

End: 982752

Strand: Reverse

Name: def [H]

Synonym: pc0803

Alternate gene names: 46446437

Gene position: 982752-982222 (Counterclockwise)

Preceding gene: 46446441

Following gene: 46446434

Centisome position: 40.7

GC content: 35.4

Gene sequence:

>531_bases
ATGAAGCTTCCTTTAGCTTATTATGGTGATTCAGTTTTGCGTAAAAAAGGGTCACAAATTGCAGAAATTAATGACACAAT
TAAACAGCTTGTTCAAGACATGATAGAAACAATGGAAGCAAATGACGGGTGTGGTTTAGCTGCTCCTCAAGTCCATCAAT
CTCTCTCATTATTTATCACTTGTATTCCTCAATACCTTGAAAACGACCAAGTTATTCCCGGTCAAGTAAGAGTGTTTATT
AATCCCAAAATCCTTTCTTACAGCCAAGAAGTTTGGGCATGTCAAGAAGCTTGTCTTTCCATTCCAGGTATGAGGGAAAC
TGTTTCCCGCCCTTTAAAAGTGACAATTCAAGCCACAGATTTAAATGGACATACTTTTACAGAAGAATTTGCAGGTTTTG
ATGCCCATGTGATTATGCACGAAAATGATCATGTCAATGGAGTTTTATACATAGATCGTTTACCCTCTAAACGCAAAAAA
GGCATTGAAAAATTCTTAAGAGAAATCAAAAAGAAATATAGTAAAAATTAA

Upstream 100 bases:

>100_bases
AAATAGTTTACAAAAAATAGCATGTAAATTTCAGTTTAAAATACTATAAATTCGAATTTTATGTTACGATGAAACTAATA
TTAAACGAGAGAAATAAATT

Downstream 100 bases:

>100_bases
ACTTTTAATCTTTCTAAAAGTTTTAATTTATTCGAAAATAAATTTATCATTAAATCATTAAATGAACATTCCGTGAATGA
ATGATATCTTAGCTCAAAAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MKLPLAYYGDSVLRKKGSQIAEINDTIKQLVQDMIETMEANDGCGLAAPQVHQSLSLFITCIPQYLENDQVIPGQVRVFI
NPKILSYSQEVWACQEACLSIPGMRETVSRPLKVTIQATDLNGHTFTEEFAGFDAHVIMHENDHVNGVLYIDRLPSKRKK
GIEKFLREIKKKYSKN

Sequences:

>Translated_176_residues
MKLPLAYYGDSVLRKKGSQIAEINDTIKQLVQDMIETMEANDGCGLAAPQVHQSLSLFITCIPQYLENDQVIPGQVRVFI
NPKILSYSQEVWACQEACLSIPGMRETVSRPLKVTIQATDLNGHTFTEEFAGFDAHVIMHENDHVNGVLYIDRLPSKRKK
GIEKFLREIKKKYSKN
>Mature_176_residues
MKLPLAYYGDSVLRKKGSQIAEINDTIKQLVQDMIETMEANDGCGLAAPQVHQSLSLFITCIPQYLENDQVIPGQVRVFI
NPKILSYSQEVWACQEACLSIPGMRETVSRPLKVTIQATDLNGHTFTEEFAGFDAHVIMHENDHVNGVLYIDRLPSKRKK
GIEKFLREIKKKYSKN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=30.4347826086957, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=39.7515527950311, Blast_Score=91, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.0126582278481, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19938; Mature: 19938

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPLAYYGDSVLRKKGSQIAEINDTIKQLVQDMIETMEANDGCGLAAPQVHQSLSLFIT
CCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
CIPQYLENDQVIPGQVRVFINPKILSYSQEVWACQEACLSIPGMRETVSRPLKVTIQATD
HHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEEEEC
LNGHTFTEEFAGFDAHVIMHENDHVNGVLYIDRLPSKRKKGIEKFLREIKKKYSKN
CCCCCCHHHHCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLPLAYYGDSVLRKKGSQIAEINDTIKQLVQDMIETMEANDGCGLAAPQVHQSLSLFIT
CCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
CIPQYLENDQVIPGQVRVFINPKILSYSQEVWACQEACLSIPGMRETVSRPLKVTIQATD
HHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEEEEC
LNGHTFTEEFAGFDAHVIMHENDHVNGVLYIDRLPSKRKKGIEKFLREIKKKYSKN
CCCCCCHHHHCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]