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The map label for this gene is tgt

Identifier: 46446428

GI number: 46446428

Start: 974519

End: 975682

Strand: Reverse

Name: tgt

Synonym: pc0794

Alternate gene names: 46446428

Gene position: 975682-974519 (Counterclockwise)

Preceding gene: 46446429

Following gene: 46446427

Centisome position: 40.41

GC content: 40.03

Gene sequence:

>1164_bases
TTGTTTTACTCCTTTTGTAAAGTGGGCCAATACATGACGTATTTTAAATTCGAACTTATCCATCGCTCTAAAAAATCACG
CGCTCGCGTTGGTCGCATTCATACCCCTCACGGAATAATTGATACTCCCAATTTTGTTGCCGTCGGAACTAATGGAACAG
TTAAAGCTTTGAACAACACCATGTTACATGACATCGGATTACAACTGATGTTTTGTAATACTTATCATATGATGCTTCAA
CCTGGAACTGATATTGTCAGACAAGCAGGCGGTTTACATTCTTTTATCCAACGAAAACTTCCGATCATTACCGATTCAGG
AGGTTTCCAAGTATTTAGTCTAGCTTATGGCTCTGTCGCTGATGAATTAAAGAGTAGGGGAACCAAAAAGCAAGGGGGAT
GTGTTCTTAAAATTACGGAAGAAGGGGTCTTATTTCGTTCTTATCGAGACGGCTCTAAGGTTTTATTAACTCCCGAAAGT
TCAATTAAAGCTCAAAAAGACTTGGGAGCTGACATTATCATTCCTTTTGATGAGCTCCCCCCTTATCATATAGCCCGCGA
TGCCTTAAAAAAATCTTTAGATCGAACTCATCGTTGGGAAAAACGATCTTTAGAGGCTCACTTAAAAAATCCTCAAGAGC
AAGCCATGTACGCCGTGGTTCACGGAGGAATTGATCCAGATTTACGAAAAGAAAGCTGTGCTATTCTAACAGAACTTCCT
TTTGATGGATTTGCAGTCGGGGGAAGCATGGGTAAAACCAAAGACGAAATGCATACACTTCTGTCTCTCACCCTCCCTCT
TCTGCCTGAAGATAAACCAAATCACCTTTTAGGAATTGGAGACCTTCCATCTATCGAGCGAAGCGTTCCGCTTGGAATTG
ATACATTTGATAGCTCATACCCAACAAGAGCTGCTAGACATGGCATTTTATTGACTAAGCAAGGTCCATTAAATATCACC
AAATCTGAGTATGCGACAAACTTTAATTCCATTGAAAAAGGATGCCTCTGCCCTGCCTGCCATCATTTTTCTCTCGCTTA
TATTCATCATCTTTTCAAAGCTCGAGAACTAACATGTATGAGTTTAGCCACAGTCCATAACCTTTATTTTATGGTTCAAC
TCATGGAAAAGTACCGGAAACAAATTCAAGAAGATTTGATTTAG

Upstream 100 bases:

>100_bases
TAGATACATTTTTATTTAGCTTTGCTGGTTTATATGGAATTGTCTCTTCAGTGACAGACATTATTGTTGTCAGTTTTATG
GTTAAATTAATTGTAATTTT

Downstream 100 bases:

>100_bases
CAGAAGGTAAATTTATGCTAAAATATAAAAAAATTATATGACGATTGTTTAATTTGACAATGAAGCAATGTTTTTTCACT
AAAAAGTCATTAGCTTCATA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFYSFCKVGQYMTYFKFELIHRSKKSRARVGRIHTPHGIIDTPNFVAVGTNGTVKALNNTMLHDIGLQLMFCNTYHMMLQ
PGTDIVRQAGGLHSFIQRKLPIITDSGGFQVFSLAYGSVADELKSRGTKKQGGCVLKITEEGVLFRSYRDGSKVLLTPES
SIKAQKDLGADIIIPFDELPPYHIARDALKKSLDRTHRWEKRSLEAHLKNPQEQAMYAVVHGGIDPDLRKESCAILTELP
FDGFAVGGSMGKTKDEMHTLLSLTLPLLPEDKPNHLLGIGDLPSIERSVPLGIDTFDSSYPTRAARHGILLTKQGPLNIT
KSEYATNFNSIEKGCLCPACHHFSLAYIHHLFKARELTCMSLATVHNLYFMVQLMEKYRKQIQEDLI

Sequences:

>Translated_387_residues
MFYSFCKVGQYMTYFKFELIHRSKKSRARVGRIHTPHGIIDTPNFVAVGTNGTVKALNNTMLHDIGLQLMFCNTYHMMLQ
PGTDIVRQAGGLHSFIQRKLPIITDSGGFQVFSLAYGSVADELKSRGTKKQGGCVLKITEEGVLFRSYRDGSKVLLTPES
SIKAQKDLGADIIIPFDELPPYHIARDALKKSLDRTHRWEKRSLEAHLKNPQEQAMYAVVHGGIDPDLRKESCAILTELP
FDGFAVGGSMGKTKDEMHTLLSLTLPLLPEDKPNHLLGIGDLPSIERSVPLGIDTFDSSYPTRAARHGILLTKQGPLNIT
KSEYATNFNSIEKGCLCPACHHFSLAYIHHLFKARELTCMSLATVHNLYFMVQLMEKYRKQIQEDLI
>Mature_387_residues
MFYSFCKVGQYMTYFKFELIHRSKKSRARVGRIHTPHGIIDTPNFVAVGTNGTVKALNNTMLHDIGLQLMFCNTYHMMLQ
PGTDIVRQAGGLHSFIQRKLPIITDSGGFQVFSLAYGSVADELKSRGTKKQGGCVLKITEEGVLFRSYRDGSKVLLTPES
SIKAQKDLGADIIIPFDELPPYHIARDALKKSLDRTHRWEKRSLEAHLKNPQEQAMYAVVHGGIDPDLRKESCAILTELP
FDGFAVGGSMGKTKDEMHTLLSLTLPLLPEDKPNHLLGIGDLPSIERSVPLGIDTFDSSYPTRAARHGILLTKQGPLNIT
KSEYATNFNSIEKGCLCPACHHFSLAYIHHLFKARELTCMSLATVHNLYFMVQLMEKYRKQIQEDLI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=369, Percent_Identity=35.7723577235772, Blast_Score=255, Evalue=5e-68,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=39.4594594594595, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=33.9779005524862, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25146502, Length=204, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=34.9462365591398, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21355311, Length=222, Percent_Identity=28.3783783783784, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_PARUW (Q6MD31)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007793.1
- ProteinModelPortal:   Q6MD31
- SMR:   Q6MD31
- STRING:   Q6MD31
- GeneID:   2781409
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0794
- NMPDR:   fig|264201.1.peg.794
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK01008
- BioCyc:   CPRO264201:PC0794-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43406; Mature: 43406

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: ACT_SITE 105-105 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYSFCKVGQYMTYFKFELIHRSKKSRARVGRIHTPHGIIDTPNFVAVGTNGTVKALNNT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
MLHDIGLQLMFCNTYHMMLQPGTDIVRQAGGLHSFIQRKLPIITDSGGFQVFSLAYGSVA
HHHHHCCEEEEECEEEEEECCCHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEHHHHHH
DELKSRGTKKQGGCVLKITEEGVLFRSYRDGSKVLLTPESSIKAQKDLGADIIIPFDELP
HHHHHCCCCCCCCEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHCCCEEEEECCCCC
PYHIARDALKKSLDRTHRWEKRSLEAHLKNPQEQAMYAVVHGGIDPDLRKESCAILTELP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCHHHEEEECC
FDGFAVGGSMGKTKDEMHTLLSLTLPLLPEDKPNHLLGIGDLPSIERSVPLGIDTFDSSY
CCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
PTRAARHGILLTKQGPLNITKSEYATNFNSIEKGCLCPACHHFSLAYIHHLFKARELTCM
CCHHHHCCEEEECCCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLATVHNLYFMVQLMEKYRKQIQEDLI
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFYSFCKVGQYMTYFKFELIHRSKKSRARVGRIHTPHGIIDTPNFVAVGTNGTVKALNNT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
MLHDIGLQLMFCNTYHMMLQPGTDIVRQAGGLHSFIQRKLPIITDSGGFQVFSLAYGSVA
HHHHHCCEEEEECEEEEEECCCHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEHHHHHH
DELKSRGTKKQGGCVLKITEEGVLFRSYRDGSKVLLTPESSIKAQKDLGADIIIPFDELP
HHHHHCCCCCCCCEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHCCCEEEEECCCCC
PYHIARDALKKSLDRTHRWEKRSLEAHLKNPQEQAMYAVVHGGIDPDLRKESCAILTELP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCHHHEEEECC
FDGFAVGGSMGKTKDEMHTLLSLTLPLLPEDKPNHLLGIGDLPSIERSVPLGIDTFDSSY
CCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
PTRAARHGILLTKQGPLNITKSEYATNFNSIEKGCLCPACHHFSLAYIHHLFKARELTCM
CCHHHHCCEEEECCCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLATVHNLYFMVQLMEKYRKQIQEDLI
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA