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The map label for this gene is nfi

Identifier: 46446257

GI number: 46446257

Start: 764994

End: 765701

Strand: Reverse

Name: nfi

Synonym: pc0623

Alternate gene names: 46446257

Gene position: 765701-764994 (Counterclockwise)

Preceding gene: 46446258

Following gene: 46446255

Centisome position: 31.71

GC content: 37.01

Gene sequence:

>708_bases
ATGAGCCAACGTTTTAATTTTGACCCCAGCTTTTTACAGCCTGATTCGATAGAAAAAGCAACTATCATTCAAAAAGAACT
TGCCAACCGCATTTGTTTAGAAGATGAATTTAACACACCAGAGTTTTTCGGAGGCATGGATGTTAGCAATAATCTTTTCG
ATCCTAAACAAATCATTTATGCAACAGCCATACTTTTAGATTCTAAAATGTTATCTGTTCAATTCCATAACAGTGTATCA
CAAAGGCAAACCTTCCCCTATATTCCTGGATTTTTAGGTTTCAGAGAAGCTCCTGCACTTATTGATGCATTAGAAAGTCT
TCCAAAGCTTCCAGACATGATTTTTGTGGATGGGCAAGGAATATCTCATCCTCGAAGGTTAGGTATTGCGAGTCATATTG
GAGTGTTAGTCAATATTCCTACAATAGGAGTTGCTAAAAATATTCTTTTTGGAGAACCGAAAAAAGACTTAGGTTCCTGT
GTGGGTGATTATGTTTTTTTGTATGCTTATGGGAAAGAAATTGGAGCTCTCGTGCGAACTAAGCTTCGCTGCAAACCACT
CATTATTTCTACAGGCCATCGCGTATCTTTAAGAACAGCTATTGAATATGTATTAAACTGTGTAAAAGGATATCGTTTGC
CTGAACCAACTAGGCAAGCTCACTTAGCTGCTAATGCTTTTAGAAAACAAAGTCAAGCAGGTAATTAA

Upstream 100 bases:

>100_bases
CACAATACAAAAAAATAAACGACTCATCATTTGTTTTGATTTTCTGTGTCTATCATGTAAAATGAAACTTATTAAAAAAA
AATTGTTAGGACATTATCAA

Downstream 100 bases:

>100_bases
TATTTTTTCTTACCTTTTATATTTAAAATAATTTTTATTCATTTGGCGTTGTGGGCATTAAATTTGTATGCGATAAATAA
TCCGACTTTTTTAAAAAAAA

Product: endonuclease V (deoxyinosine 3'endonuclease)

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSQRFNFDPSFLQPDSIEKATIIQKELANRICLEDEFNTPEFFGGMDVSNNLFDPKQIIYATAILLDSKMLSVQFHNSVS
QRQTFPYIPGFLGFREAPALIDALESLPKLPDMIFVDGQGISHPRRLGIASHIGVLVNIPTIGVAKNILFGEPKKDLGSC
VGDYVFLYAYGKEIGALVRTKLRCKPLIISTGHRVSLRTAIEYVLNCVKGYRLPEPTRQAHLAANAFRKQSQAGN

Sequences:

>Translated_235_residues
MSQRFNFDPSFLQPDSIEKATIIQKELANRICLEDEFNTPEFFGGMDVSNNLFDPKQIIYATAILLDSKMLSVQFHNSVS
QRQTFPYIPGFLGFREAPALIDALESLPKLPDMIFVDGQGISHPRRLGIASHIGVLVNIPTIGVAKNILFGEPKKDLGSC
VGDYVFLYAYGKEIGALVRTKLRCKPLIISTGHRVSLRTAIEYVLNCVKGYRLPEPTRQAHLAANAFRKQSQAGN
>Mature_234_residues
SQRFNFDPSFLQPDSIEKATIIQKELANRICLEDEFNTPEFFGGMDVSNNLFDPKQIIYATAILLDSKMLSVQFHNSVSQ
RQTFPYIPGFLGFREAPALIDALESLPKLPDMIFVDGQGISHPRRLGIASHIGVLVNIPTIGVAKNILFGEPKKDLGSCV
GDYVFLYAYGKEIGALVRTKLRCKPLIISTGHRVSLRTAIEYVLNCVKGYRLPEPTRQAHLAANAFRKQSQAGN

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=211, Percent_Identity=33.6492890995261, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI257467548, Length=137, Percent_Identity=35.7664233576642, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI257467550, Length=137, Percent_Identity=35.7664233576642, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI87082357, Length=206, Percent_Identity=46.6019417475728, Blast_Score=175, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_PARUW (Q6MDK2)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007622.1
- ProteinModelPortal:   Q6MDK2
- STRING:   Q6MDK2
- GeneID:   2780638
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0623
- NMPDR:   fig|264201.1.peg.623
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   SVGHRIG
- PhylomeDB:   Q6MDK2
- BioCyc:   CPRO264201:PC0623-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 26158; Mature: 26026

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRFNFDPSFLQPDSIEKATIIQKELANRICLEDEFNTPEFFGGMDVSNNLFDPKQIIY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
ATAILLDSKMLSVQFHNSVSQRQTFPYIPGFLGFREAPALIDALESLPKLPDMIFVDGQG
HHHHHHHHHHEEEEECCCHHHCCCCCCCCCHHCHHHHHHHHHHHHHCCCCCCEEEECCCC
ISHPRRLGIASHIGVLVNIPTIGVAKNILFGEPKKDLGSCVGDYVFLYAYGKEIGALVRT
CCCHHHHHHHHHCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRCKPLIISTGHRVSLRTAIEYVLNCVKGYRLPEPTRQAHLAANAFRKQSQAGN
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQRFNFDPSFLQPDSIEKATIIQKELANRICLEDEFNTPEFFGGMDVSNNLFDPKQIIY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
ATAILLDSKMLSVQFHNSVSQRQTFPYIPGFLGFREAPALIDALESLPKLPDMIFVDGQG
HHHHHHHHHHEEEEECCCHHHCCCCCCCCCHHCHHHHHHHHHHHHHCCCCCCEEEECCCC
ISHPRRLGIASHIGVLVNIPTIGVAKNILFGEPKKDLGSCVGDYVFLYAYGKEIGALVRT
CCCHHHHHHHHHCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRCKPLIISTGHRVSLRTAIEYVLNCVKGYRLPEPTRQAHLAANAFRKQSQAGN
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA