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The map label for this gene is dapF [H]

Identifier: 46446076

GI number: 46446076

Start: 571137

End: 571946

Strand: Reverse

Name: dapF [H]

Synonym: pc0442

Alternate gene names: 46446076

Gene position: 571946-571137 (Counterclockwise)

Preceding gene: 46446077

Following gene: 46446075

Centisome position: 23.69

GC content: 38.52

Gene sequence:

>810_bases
ATGCAGTGGTCTTTTACTAAATATGTGGGATGTGGAAATGATTTCATTCTTTTTGATAATCGAAATGGTAAATTTCCATT
TTCCATCCCTCTTATTCAACAGCTTTGCCATCGGCAATGGGGTGTTGGAGCTGATGGGATTATCCTTTTAGAAAACTCGA
CAATTGCTCATGCCCGCATGCGCATTTTTAATGCAGATGGAAGTGAGGCCGAAATGTGTGGAAACGGTCTTCGATGTTTA
ATCGATTGGATGGAATCTTTAAATATTTTGCATTCCATTTATTCTATAGAAACAAATAACCATGTGCTAAAAGCTTGGAA
AAGTGCATCTAATATCACTATTGAAATGGGCTCCCCCAACAAATTAAATTGGAATGTGCCTATTTCATTTGAGTCAAAAA
TCTATTATGTGCACTCATTAAATACAGGGGTTCCACACATTATCTTATTCGCTGAAAACATTGAACAATTTCCGTTAGAA
CGAATGGGGCCATTCATTCGTTATCATCCGTTATGGAAACCTGACGGAACTAACTTCTCAATTGTACAGCATCTCCATGG
TCAAACTTTCATGATTCGCACTTATGAAAGAGGGGTAGAAGCAGAGACATTAGCTTGTGGGACGGGAGCAACAGCAGCGG
CTCTAGCAGCAGCTTTTCAGTATCGTTTAAGTGCTCCCATATCAATGCAAACTCGATCGGGAATTTGTTTAGAAATTGGT
TTCACGTTGCTTAATCAAAAATTTACAAATGTGACAATGACAGGTCCAGCATTGGCCGTTTTCAACGGCTCAATTAAATT
AGAATCATAA

Upstream 100 bases:

>100_bases
ATCGAGATACATGGATGACAGCACAAGAAGCCTTAGAATTTGGACTACTCGATAAAGTGATTAATAGCTTTGAAGAGATT
GAATCCACTTAAAAAAACTT

Downstream 100 bases:

>100_bases
CCGAAACTTTAAGAAAAAAATTTAACGCTTAATTGATAAAAATATGCTAGTTAAAAAGCTTTTCGATTGCTTGTAACTCA
ATCAATTCGATACAATCCCA

Product: putative diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MQWSFTKYVGCGNDFILFDNRNGKFPFSIPLIQQLCHRQWGVGADGIILLENSTIAHARMRIFNADGSEAEMCGNGLRCL
IDWMESLNILHSIYSIETNNHVLKAWKSASNITIEMGSPNKLNWNVPISFESKIYYVHSLNTGVPHIILFAENIEQFPLE
RMGPFIRYHPLWKPDGTNFSIVQHLHGQTFMIRTYERGVEAETLACGTGATAAALAAAFQYRLSAPISMQTRSGICLEIG
FTLLNQKFTNVTMTGPALAVFNGSIKLES

Sequences:

>Translated_269_residues
MQWSFTKYVGCGNDFILFDNRNGKFPFSIPLIQQLCHRQWGVGADGIILLENSTIAHARMRIFNADGSEAEMCGNGLRCL
IDWMESLNILHSIYSIETNNHVLKAWKSASNITIEMGSPNKLNWNVPISFESKIYYVHSLNTGVPHIILFAENIEQFPLE
RMGPFIRYHPLWKPDGTNFSIVQHLHGQTFMIRTYERGVEAETLACGTGATAAALAAAFQYRLSAPISMQTRSGICLEIG
FTLLNQKFTNVTMTGPALAVFNGSIKLES
>Mature_269_residues
MQWSFTKYVGCGNDFILFDNRNGKFPFSIPLIQQLCHRQWGVGADGIILLENSTIAHARMRIFNADGSEAEMCGNGLRCL
IDWMESLNILHSIYSIETNNHVLKAWKSASNITIEMGSPNKLNWNVPISFESKIYYVHSLNTGVPHIILFAENIEQFPLE
RMGPFIRYHPLWKPDGTNFSIVQHLHGQTFMIRTYERGVEAETLACGTGATAAALAAAFQYRLSAPISMQTRSGICLEIG
FTLLNQKFTNVTMTGPALAVFNGSIKLES

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=29.6428571428571, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30111; Mature: 30111

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWSFTKYVGCGNDFILFDNRNGKFPFSIPLIQQLCHRQWGVGADGIILLENSTIAHARM
CCCCCEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEE
RIFNADGSEAEMCGNGLRCLIDWMESLNILHSIYSIETNNHVLKAWKSASNITIEMGSPN
EEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCHHEEEECCCCCEEEEECCCC
KLNWNVPISFESKIYYVHSLNTGVPHIILFAENIEQFPLERMGPFIRYHPLWKPDGTNFS
EEEEEEEEEECCEEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCEEECCCCCCCCCCHH
IVQHLHGQTFMIRTYERGVEAETLACGTGATAAALAAAFQYRLSAPISMQTRSGICLEIG
HHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEEC
FTLLNQKFTNVTMTGPALAVFNGSIKLES
HHHHCCHHCEEEECCCEEEEEECEEEECC
>Mature Secondary Structure
MQWSFTKYVGCGNDFILFDNRNGKFPFSIPLIQQLCHRQWGVGADGIILLENSTIAHARM
CCCCCEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEE
RIFNADGSEAEMCGNGLRCLIDWMESLNILHSIYSIETNNHVLKAWKSASNITIEMGSPN
EEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCHHEEEECCCCCEEEEECCCC
KLNWNVPISFESKIYYVHSLNTGVPHIILFAENIEQFPLERMGPFIRYHPLWKPDGTNFS
EEEEEEEEEECCEEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCEEECCCCCCCCCCHH
IVQHLHGQTFMIRTYERGVEAETLACGTGATAAALAAAFQYRLSAPISMQTRSGICLEIG
HHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEEC
FTLLNQKFTNVTMTGPALAVFNGSIKLES
HHHHCCHHCEEEECCCEEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA