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The map label for this gene is rlmN1

Identifier: 46445973

GI number: 46445973

Start: 462413

End: 463489

Strand: Reverse

Name: rlmN1

Synonym: pc0339

Alternate gene names: 46445973

Gene position: 463489-462413 (Counterclockwise)

Preceding gene: 46445976

Following gene: 46445972

Centisome position: 19.2

GC content: 34.82

Gene sequence:

>1077_bases
GTGAAGATTCCTCCTATATCAATTTTATCGCATACCTCTGAAAGCTATGCCAACGCGATTAGTCATGAACTTGGTAAAGG
CTTTCAACACGCAAAACTCGTTTATCAAGAATGGTTTCGCAGAGGTAATATCAGTGGACTAAATCCAGCATTTAAAAATG
CTCAAGCCCTTTTACAAAATATTCTTTCTCTAACCGATTTCTCTTTTTTACCCATTTCACAAAATTTGACAGATGGTCAA
ACAGGAAAATTTTTAATTAAAACTATAGATGACTTAGATATCAAGTCTGTATTAATTCCCATGCAAGCAGGTGGGACTCT
ATGCATTTCCTCACAAATTGGATGTCAAATGGGATGTGCATTTTGTGAAACAGGTCGAATGGGTCTTTTGAGAAATTTAA
CGACCCAAGAAATTCTCAGTCAACTTTTCATTGCGAAATTCCGTTTACATTTTTCAGTTAGAAATATTGTTTTCATGGGA
ATGGGAGAACCTTTTGATAATTATGATACTGTCATGCATGCATTTCGCATTTTAACAGATTCCCATGGATTTGGTTTGGG
AAATAATCGTATTACTATTTCCACGAGTGGTTGTTTAGAAGGAATTTACCGATTATTGCAAGAAACAACCCCACTCCCTA
ATTTAGCGGTTTCTTTAAATGCTCCCAATGATGAGTTGCGTAACAAGCTCATGCCTATTAACAAAAAATATCCCTTAAAA
GAGCTTTATCAAGCTATCTATGATTTTTGTAAACAAACTAGTAAACAAGTTTTAATTGCTTATGTTCTCATTAAGGAGCA
AAATGATTCAATCGAGCACGCTAAACAATTAACCAATTTTTTATCCGGACTCAATGTTAAAATTAATTTGATTCCCTACA
ATCCCCAAAGTCGTGACCGATTTCAATCTCCTGAGCAATCCACTCTTGAAAATTTTACCAGTTATCTTCGAGAAAAAGGA
TTTTATACACTTCTAAGGCAAACTAAAGGACAAAAAATTATGGCAGCTTGTGGACAGTTAGGCAACCTTGAGCTGAAACG
TAAAAAACCCTTTATTCTGCCGATACTAAAAGAATAA

Upstream 100 bases:

>100_bases
ATTAATTCTTATCGCACCGATAGCTCAATGGATAGAGTACCCGGCTTCGAACCGGGTGGTTAGAGGTTCGAGCCCTCTTC
GGTGCATAGTACATCATAAC

Downstream 100 bases:

>100_bases
AAAAGTTAGATTGACTATCAATTCTTAAACTTTTTATCATAAAATCCAAAGAATAAACAGCTACCGTGTATGAATCAATT
TCTCTTTGCAACGACAAATC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase 1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKIPPISILSHTSESYANAISHELGKGFQHAKLVYQEWFRRGNISGLNPAFKNAQALLQNILSLTDFSFLPISQNLTDGQ
TGKFLIKTIDDLDIKSVLIPMQAGGTLCISSQIGCQMGCAFCETGRMGLLRNLTTQEILSQLFIAKFRLHFSVRNIVFMG
MGEPFDNYDTVMHAFRILTDSHGFGLGNNRITISTSGCLEGIYRLLQETTPLPNLAVSLNAPNDELRNKLMPINKKYPLK
ELYQAIYDFCKQTSKQVLIAYVLIKEQNDSIEHAKQLTNFLSGLNVKINLIPYNPQSRDRFQSPEQSTLENFTSYLREKG
FYTLLRQTKGQKIMAACGQLGNLELKRKKPFILPILKE

Sequences:

>Translated_358_residues
MKIPPISILSHTSESYANAISHELGKGFQHAKLVYQEWFRRGNISGLNPAFKNAQALLQNILSLTDFSFLPISQNLTDGQ
TGKFLIKTIDDLDIKSVLIPMQAGGTLCISSQIGCQMGCAFCETGRMGLLRNLTTQEILSQLFIAKFRLHFSVRNIVFMG
MGEPFDNYDTVMHAFRILTDSHGFGLGNNRITISTSGCLEGIYRLLQETTPLPNLAVSLNAPNDELRNKLMPINKKYPLK
ELYQAIYDFCKQTSKQVLIAYVLIKEQNDSIEHAKQLTNFLSGLNVKINLIPYNPQSRDRFQSPEQSTLENFTSYLREKG
FYTLLRQTKGQKIMAACGQLGNLELKRKKPFILPILKE
>Mature_358_residues
MKIPPISILSHTSESYANAISHELGKGFQHAKLVYQEWFRRGNISGLNPAFKNAQALLQNILSLTDFSFLPISQNLTDGQ
TGKFLIKTIDDLDIKSVLIPMQAGGTLCISSQIGCQMGCAFCETGRMGLLRNLTTQEILSQLFIAKFRLHFSVRNIVFMG
MGEPFDNYDTVMHAFRILTDSHGFGLGNNRITISTSGCLEGIYRLLQETTPLPNLAVSLNAPNDELRNKLMPINKKYPLK
ELYQAIYDFCKQTSKQVLIAYVLIKEQNDSIEHAKQLTNFLSGLNVKINLIPYNPQSRDRFQSPEQSTLENFTSYLREKG
FYTLLRQTKGQKIMAACGQLGNLELKRKKPFILPILKE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=287, Percent_Identity=38.3275261324042, Blast_Score=198, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN1_PARUW (Q6MED6)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007338.1
- ProteinModelPortal:   Q6MED6
- STRING:   Q6MED6
- GeneID:   2780130
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0339
- NMPDR:   fig|264201.1.peg.339
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   THEMEAL
- ProtClustDB:   CLSK2462923
- BioCyc:   CPRO264201:PC0339-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40410; Mature: 40410

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPPISILSHTSESYANAISHELGKGFQHAKLVYQEWFRRGNISGLNPAFKNAQALLQN
CCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ILSLTDFSFLPISQNLTDGQTGKFLIKTIDDLDIKSVLIPMQAGGTLCISSQIGCQMGCA
HHHHHCCCEECCCCCCCCCCCCCEEEEHHCCCCHHHEEEEECCCCCEEEECCCCHHHHHH
FCETGRMGLLRNLTTQEILSQLFIAKFRLHFSVRNIVFMGMGEPFDNYDTVMHAFRILTD
HHCCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEHHEEEEECCCCCCHHHHHHHHHHHHHC
SHGFGLGNNRITISTSGCLEGIYRLLQETTPLPNLAVSLNAPNDELRNKLMPINKKYPLK
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCCCHH
ELYQAIYDFCKQTSKQVLIAYVLIKEQNDSIEHAKQLTNFLSGLNVKINLIPYNPQSRDR
HHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
FQSPEQSTLENFTSYLREKGFYTLLRQTKGQKIMAACGQLGNLELKRKKPFILPILKE
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCEEECCCCEEECCCCC
>Mature Secondary Structure
MKIPPISILSHTSESYANAISHELGKGFQHAKLVYQEWFRRGNISGLNPAFKNAQALLQN
CCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ILSLTDFSFLPISQNLTDGQTGKFLIKTIDDLDIKSVLIPMQAGGTLCISSQIGCQMGCA
HHHHHCCCEECCCCCCCCCCCCCEEEEHHCCCCHHHEEEEECCCCCEEEECCCCHHHHHH
FCETGRMGLLRNLTTQEILSQLFIAKFRLHFSVRNIVFMGMGEPFDNYDTVMHAFRILTD
HHCCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEHHEEEEECCCCCCHHHHHHHHHHHHHC
SHGFGLGNNRITISTSGCLEGIYRLLQETTPLPNLAVSLNAPNDELRNKLMPINKKYPLK
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCCCHH
ELYQAIYDFCKQTSKQVLIAYVLIKEQNDSIEHAKQLTNFLSGLNVKINLIPYNPQSRDR
HHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
FQSPEQSTLENFTSYLREKGFYTLLRQTKGQKIMAACGQLGNLELKRKKPFILPILKE
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA