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The map label for this gene is nifS [H]

Identifier: 46445796

GI number: 46445796

Start: 241167

End: 242348

Strand: Direct

Name: nifS [H]

Synonym: pc0162

Alternate gene names: 46445796

Gene position: 241167-242348 (Clockwise)

Preceding gene: 46445795

Following gene: 46445797

Centisome position: 9.99

GC content: 38.92

Gene sequence:

>1182_bases
ATGGTTAAATTGCCTATTTATTTAGATAACAGTACAACTAGTCGTCCATCTGATTTATCAATTAGCCAAATGATGCCTTA
TTTAACAGATCGTTGGGGTCTTCCCTCAGCACCGCATCAAAAAGGTCAAGAGCTTTACCCAGTCTTGAAAGAGTGTTATA
AAATTCTTTATGCATTTTTGGGAGCTCAAGAAGCAGATCAATTTGTATTGACCTCTTCAGGAGCAGAAGCTGTTAATCAG
GTGATTTCTTCCGTTTATAGAGATGTCACGCTCCCAAGTGGCAAAAATCAATTTTTAACTTCTAATGCTGATGAAGCACC
AGCGTTAATGGCAATGGCACGCTTAGAGCCCTTAGGTTGCGTTGCAAAAATAATTGAATCGAATCAATATGGTATTGTAA
CTGCAGAAGCTCTAGCCGAGGCTATTTCACCGCGTACTGCGTTGATTTCTCTTTCTTGGGCTAATGGTTTGACAGGAGTT
GTCCAGCCACTGGGAGAAATAATTGCGCTCTGTAAACAGCGTGACATCCGTCTTCATTTAGATGCCACGCATGTATTGGG
TAAATTATTTTATGAGTTTGATGAACTTTCTGTAGATTTTATGACGTTTAATGGAGATCAGCTTCATGCTCCCAAAGGAA
CGGGGGGATTATATATTAAACAAGGAATTAAATGTTCTCCATTGATTGCTGGAGGTTCTGATCAAGCTGGGCTACGGGCT
GGAGGTTTAAATATGGCATCATTAGTGGCTCTAGCTTATGCTGCCAAAGAAACTTTAGAATGTCGTGATTTGCTTTGTAC
TGAGACAGCAAGACTCAAACATAAGTTGGAAACAGGAATTGTGAAAGCTTATCCTCAAGCCAGAATTTGTTTTAAAGAAT
GTGAACGATTGCCTCATTGCACAACTCTTTTGTTTCCCGGCATTGCTAATGAGGCATTACTGTTCAATCTTAATCGTAAA
GGAGTTTATGCGAATATTGGAGGGGGAAATTTTCAACAACTTGGCTTACTATTAAGCGCTTGTGGCCTAGAAGAAAATTT
AGCATATAGCGCCGTTTCATTTAGTTTATCTCGCTATACAACAGAGGAAGAAATTGATCGAGCTATTGAATTGGTTGTTG
AAAGTGCTTGGCAGTTGAATCGAACTTCTTATAAATTATTTGAGACAGAGAGAAAAATTTAA

Upstream 100 bases:

>100_bases
GACAACAGATCAAGTCGTTAAGTTGGAACTGGCAACAGGGGTGCCTGTGATCTATGATTTCAATCACGATGAATATATTA
AACAACAAAAGTAGAATAGC

Downstream 100 bases:

>100_bases
TATGACTTTGCAAACACTCACTCAAAGTTTTCCGTGGACACGTTATAGCAAAAAGTTACTAGCTAAAATTGATAAACCAC
GATGTATGGGTTTTTTTAAT

Product: putative iron-sulfur cofactor synthesis protein/cysteine desulfurase nifS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVKLPIYLDNSTTSRPSDLSISQMMPYLTDRWGLPSAPHQKGQELYPVLKECYKILYAFLGAQEADQFVLTSSGAEAVNQ
VISSVYRDVTLPSGKNQFLTSNADEAPALMAMARLEPLGCVAKIIESNQYGIVTAEALAEAISPRTALISLSWANGLTGV
VQPLGEIIALCKQRDIRLHLDATHVLGKLFYEFDELSVDFMTFNGDQLHAPKGTGGLYIKQGIKCSPLIAGGSDQAGLRA
GGLNMASLVALAYAAKETLECRDLLCTETARLKHKLETGIVKAYPQARICFKECERLPHCTTLLFPGIANEALLFNLNRK
GVYANIGGGNFQQLGLLLSACGLEENLAYSAVSFSLSRYTTEEEIDRAIELVVESAWQLNRTSYKLFETERKI

Sequences:

>Translated_393_residues
MVKLPIYLDNSTTSRPSDLSISQMMPYLTDRWGLPSAPHQKGQELYPVLKECYKILYAFLGAQEADQFVLTSSGAEAVNQ
VISSVYRDVTLPSGKNQFLTSNADEAPALMAMARLEPLGCVAKIIESNQYGIVTAEALAEAISPRTALISLSWANGLTGV
VQPLGEIIALCKQRDIRLHLDATHVLGKLFYEFDELSVDFMTFNGDQLHAPKGTGGLYIKQGIKCSPLIAGGSDQAGLRA
GGLNMASLVALAYAAKETLECRDLLCTETARLKHKLETGIVKAYPQARICFKECERLPHCTTLLFPGIANEALLFNLNRK
GVYANIGGGNFQQLGLLLSACGLEENLAYSAVSFSLSRYTTEEEIDRAIELVVESAWQLNRTSYKLFETERKI
>Mature_393_residues
MVKLPIYLDNSTTSRPSDLSISQMMPYLTDRWGLPSAPHQKGQELYPVLKECYKILYAFLGAQEADQFVLTSSGAEAVNQ
VISSVYRDVTLPSGKNQFLTSNADEAPALMAMARLEPLGCVAKIIESNQYGIVTAEALAEAISPRTALISLSWANGLTGV
VQPLGEIIALCKQRDIRLHLDATHVLGKLFYEFDELSVDFMTFNGDQLHAPKGTGGLYIKQGIKCSPLIAGGSDQAGLRA
GGLNMASLVALAYAAKETLECRDLLCTETARLKHKLETGIVKAYPQARICFKECERLPHCTTLLFPGIANEALLFNLNRK
GVYANIGGGNFQQLGLLLSACGLEENLAYSAVSFSLSRYTTEEEIDRAIELVVESAWQLNRTSYKLFETERKI

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=27.2727272727273, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=24.0291262135922, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI48994898, Length=392, Percent_Identity=29.8469387755102, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1787970, Length=237, Percent_Identity=24.0506329113924, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=27.5324675324675, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=24.9221183800623, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319831, Length=374, Percent_Identity=25.9358288770053, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=27.2727272727273, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017773
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43028; Mature: 43028

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLPIYLDNSTTSRPSDLSISQMMPYLTDRWGLPSAPHQKGQELYPVLKECYKILYAFL
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAQEADQFVLTSSGAEAVNQVISSVYRDVTLPSGKNQFLTSNADEAPALMAMARLEPLGC
CCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
VAKIIESNQYGIVTAEALAEAISPRTALISLSWANGLTGVVQPLGEIIALCKQRDIRLHL
HHHHHHCCCCCEEEHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
DATHVLGKLFYEFDELSVDFMTFNGDQLHAPKGTGGLYIKQGIKCSPLIAGGSDQAGLRA
CHHHHHHHHHHHHHHCEEEEEEECCCEEECCCCCCCEEEECCCCCCEEEECCCCCCCCEE
GGLNMASLVALAYAAKETLECRDLLCTETARLKHKLETGIVKAYPQARICFKECERLPHC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
TTLLFPGIANEALLFNLNRKGVYANIGGGNFQQLGLLLSACGLEENLAYSAVSFSLSRYT
HHHHCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
TEEEIDRAIELVVESAWQLNRTSYKLFETERKI
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure
MVKLPIYLDNSTTSRPSDLSISQMMPYLTDRWGLPSAPHQKGQELYPVLKECYKILYAFL
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAQEADQFVLTSSGAEAVNQVISSVYRDVTLPSGKNQFLTSNADEAPALMAMARLEPLGC
CCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
VAKIIESNQYGIVTAEALAEAISPRTALISLSWANGLTGVVQPLGEIIALCKQRDIRLHL
HHHHHHCCCCCEEEHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
DATHVLGKLFYEFDELSVDFMTFNGDQLHAPKGTGGLYIKQGIKCSPLIAGGSDQAGLRA
CHHHHHHHHHHHHHHCEEEEEEECCCEEECCCCCCCEEEECCCCCCEEEECCCCCCCCEE
GGLNMASLVALAYAAKETLECRDLLCTETARLKHKLETGIVKAYPQARICFKECERLPHC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
TTLLFPGIANEALLFNLNRKGVYANIGGGNFQQLGLLLSACGLEENLAYSAVSFSLSRYT
HHHHCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
TEEEIDRAIELVVESAWQLNRTSYKLFETERKI
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA