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The map label for this gene is lpdA [H]

Identifier: 46445785

GI number: 46445785

Start: 227479

End: 228888

Strand: Direct

Name: lpdA [H]

Synonym: pc0151

Alternate gene names: 46445785

Gene position: 227479-228888 (Clockwise)

Preceding gene: 46445783

Following gene: 46445786

Centisome position: 9.42

GC content: 41.06

Gene sequence:

>1410_bases
ATGATGGAAATAAAAAAATTCGATGTGGCTGTTCTTGGAGGAGGCCCTGGCGGCTATCCAGCTGCGATTCGAGCAGCACA
ACGAGGTAAATCTGTTGCATTGATTGAAGCCAAAGAATTGGGTGGTACTTGTTTAAATAGGGGGTGTATTCCTTCAAAAG
CTCTAATTGCTGGAGCAGAAGTTGTTGATCGCATCCATGAAGCGAAAGAATTTGGAATTCATGTGGGTGAAGTTAATATC
GATTATGCTCAACTTGCTTTCCATAAAGACCGAGTGATTGAACGGATGCGTAAAGGCTTAGAAGGGCTTATGGCAACTAA
TAAAATTACTGTTTTTAAAGGATTTGGACAATTTATTTCCCCTCGAGAAATTAAAATCAAAGGTCAGGATAATACAAGTA
TTACCGCCGATCAAATTATCATCGCGACAGGATCAGAACCGCGTAATATTTCAGCTTTCCCATTTGACTATGAACGCATT
CATGACTCTACCTCACTGCTAGCCATGAAAACTCTTCCTGAAAAACTTATTATCGTTGGAGGGGGAGTCATTGGCTGTGA
ATTTGCCTCTCTTTATGCCACTCTTGGTGTCCATGTGACAATCTTAGAATTGTTGCCACGTTTAATTTCGTCTGAGGCTA
TTGAAGTATCACAAGCATTGACAAAAGCATTTATTAAGCGAGGTATTCAAGTAGAAACAGGGGTAAAAGTTCAAAAAATT
GAACAAGTTGAGATGGGAATACAAGTTCAAGTAGAAGAAGGAAAACATTACGTTGCTGACTGTTGTTTAGTCGCAGTAGG
TCGTTCTCTCAATACTAAAAACATTGGACTTGACAAAGCAGGTATTTTAGTTCAAGAAAATGGGATGATTGCTGTCAATG
ATAAAATGCAAACAAACGTTGATGGTATTTACGCGGTAGGGGACATTGCATCGAAGTGGTGGTTAGCTCACGTTGCTTCT
CACCAAGGCCTTATAGCAGCAGACCAAATTTGTGGAAAAAGTAGTCGTATGTATTATCACGCGATTCCTTCCGTAATCTT
TACTCATCCTGAAATCGCAACAGTCGGATTATCACTTGAAGAAGCGACGAAAAGAGGATATAAAGCTAAGTTGGCGAGCT
TTCCTTTTCAGGCGCTTGGAAAATCTCAAGCTGCTTTACAGGCAGAAGGATTTGCCCAAATTGTTGTCGAAGAAAAATCA
GGACAGATTCTTGGCGCTCAAGTTGTTGGTTATGAAGCCTCTGTAATGATTGCAGAAATGACTCTCGCTATTGCGAACGA
ATTGACCGTGGAATGTGTGGCAGAAACCATCCATGCCCATCCTACTCTACCGGAAGCTTGGCTAGAATCAGCACTACTAG
GGGAAGGTTTACCCTTGCATTTACCCCCTAAAACGCAAAAAAAGGGATAA

Upstream 100 bases:

>100_bases
GAATTGACAATTGTATATGCTAAACATCTCATTTTGGAGTTGATGCTAATTAAAAATTTAGGAAGTTTTAACTCTAGTGA
CAAGTTTATCGAAGGAGAGA

Downstream 100 bases:

>100_bases
GATTCAATTTTTAAAGGACTGCTGTGATGGAAAACTCTCCAAAAACTCGTCGCTTAAATATCCTACCAGATAATCCTGAA
AATACTGGAGATGGTGTGGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MMEIKKFDVAVLGGGPGGYPAAIRAAQRGKSVALIEAKELGGTCLNRGCIPSKALIAGAEVVDRIHEAKEFGIHVGEVNI
DYAQLAFHKDRVIERMRKGLEGLMATNKITVFKGFGQFISPREIKIKGQDNTSITADQIIIATGSEPRNISAFPFDYERI
HDSTSLLAMKTLPEKLIIVGGGVIGCEFASLYATLGVHVTILELLPRLISSEAIEVSQALTKAFIKRGIQVETGVKVQKI
EQVEMGIQVQVEEGKHYVADCCLVAVGRSLNTKNIGLDKAGILVQENGMIAVNDKMQTNVDGIYAVGDIASKWWLAHVAS
HQGLIAADQICGKSSRMYYHAIPSVIFTHPEIATVGLSLEEATKRGYKAKLASFPFQALGKSQAALQAEGFAQIVVEEKS
GQILGAQVVGYEASVMIAEMTLAIANELTVECVAETIHAHPTLPEAWLESALLGEGLPLHLPPKTQKKG

Sequences:

>Translated_469_residues
MMEIKKFDVAVLGGGPGGYPAAIRAAQRGKSVALIEAKELGGTCLNRGCIPSKALIAGAEVVDRIHEAKEFGIHVGEVNI
DYAQLAFHKDRVIERMRKGLEGLMATNKITVFKGFGQFISPREIKIKGQDNTSITADQIIIATGSEPRNISAFPFDYERI
HDSTSLLAMKTLPEKLIIVGGGVIGCEFASLYATLGVHVTILELLPRLISSEAIEVSQALTKAFIKRGIQVETGVKVQKI
EQVEMGIQVQVEEGKHYVADCCLVAVGRSLNTKNIGLDKAGILVQENGMIAVNDKMQTNVDGIYAVGDIASKWWLAHVAS
HQGLIAADQICGKSSRMYYHAIPSVIFTHPEIATVGLSLEEATKRGYKAKLASFPFQALGKSQAALQAEGFAQIVVEEKS
GQILGAQVVGYEASVMIAEMTLAIANELTVECVAETIHAHPTLPEAWLESALLGEGLPLHLPPKTQKKG
>Mature_469_residues
MMEIKKFDVAVLGGGPGGYPAAIRAAQRGKSVALIEAKELGGTCLNRGCIPSKALIAGAEVVDRIHEAKEFGIHVGEVNI
DYAQLAFHKDRVIERMRKGLEGLMATNKITVFKGFGQFISPREIKIKGQDNTSITADQIIIATGSEPRNISAFPFDYERI
HDSTSLLAMKTLPEKLIIVGGGVIGCEFASLYATLGVHVTILELLPRLISSEAIEVSQALTKAFIKRGIQVETGVKVQKI
EQVEMGIQVQVEEGKHYVADCCLVAVGRSLNTKNIGLDKAGILVQENGMIAVNDKMQTNVDGIYAVGDIASKWWLAHVAS
HQGLIAADQICGKSSRMYYHAIPSVIFTHPEIATVGLSLEEATKRGYKAKLASFPFQALGKSQAALQAEGFAQIVVEEKS
GQILGAQVVGYEASVMIAEMTLAIANELTVECVAETIHAHPTLPEAWLESALLGEGLPLHLPPKTQKKG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=36.0262008733624, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=29.1938997821351, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=27.1334792122538, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=27.1334792122538, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=27.1334792122538, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=27.1334792122538, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=27.1334792122538, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI291045266, Length=427, Percent_Identity=28.8056206088993, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=26.8131868131868, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=317, Percent_Identity=30.2839116719243, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=37.6321353065539, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=30.4824561403509, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=29.0993071593533, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=27.7533039647577, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=35.0877192982456, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=26.6112266112266, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=460, Percent_Identity=26.0869565217391, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=460, Percent_Identity=26.0869565217391, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=372, Percent_Identity=26.0752688172043, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=36.2869198312236, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.3870967741935, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=25.4736842105263, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=36.7032967032967, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=28.2426778242678, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=28.2426778242678, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=28.2426778242678, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=25.6900212314225, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50429; Mature: 50429

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEIKKFDVAVLGGGPGGYPAAIRAAQRGKSVALIEAKELGGTCLNRGCIPSKALIAGAE
CCCEEEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEEHHCCCHHHCCCCCCCHHHHHHHH
VVDRIHEAKEFGIHVGEVNIDYAQLAFHKDRVIERMRKGLEGLMATNKITVFKGFGQFIS
HHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PREIKIKGQDNTSITADQIIIATGSEPRNISAFPFDYERIHDSTSLLAMKTLPEKLIIVG
CCEEEEECCCCCCEEECEEEEEECCCCCCCCCCCCCHHHHCCCHHEEEEHHCCCEEEEEE
GGVIGCEFASLYATLGVHVTILELLPRLISSEAIEVSQALTKAFIKRGIQVETGVKVQKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
EQVEMGIQVQVEEGKHYVADCCLVAVGRSLNTKNIGLDKAGILVQENGMIAVNDKMQTNV
HHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCC
DGIYAVGDIASKWWLAHVASHQGLIAADQICGKSSRMYYHAIPSVIFTHPEIATVGLSLE
CCEEEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCHHHCCCCCCEEECCCHH
EATKRGYKAKLASFPFQALGKSQAALQAEGFAQIVVEEKSGQILGAQVVGYEASVMIAEM
HHHHCCCHHHHHCCCHHHHCCCHHHHHHCCEEEEEEECCCCCEEEEEEECCCCEEHHHHH
TLAIANELTVECVAETIHAHPTLPEAWLESALLGEGLPLHLPPKTQKKG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure
MMEIKKFDVAVLGGGPGGYPAAIRAAQRGKSVALIEAKELGGTCLNRGCIPSKALIAGAE
CCCEEEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEEHHCCCHHHCCCCCCCHHHHHHHH
VVDRIHEAKEFGIHVGEVNIDYAQLAFHKDRVIERMRKGLEGLMATNKITVFKGFGQFIS
HHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PREIKIKGQDNTSITADQIIIATGSEPRNISAFPFDYERIHDSTSLLAMKTLPEKLIIVG
CCEEEEECCCCCCEEECEEEEEECCCCCCCCCCCCCHHHHCCCHHEEEEHHCCCEEEEEE
GGVIGCEFASLYATLGVHVTILELLPRLISSEAIEVSQALTKAFIKRGIQVETGVKVQKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
EQVEMGIQVQVEEGKHYVADCCLVAVGRSLNTKNIGLDKAGILVQENGMIAVNDKMQTNV
HHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCC
DGIYAVGDIASKWWLAHVASHQGLIAADQICGKSSRMYYHAIPSVIFTHPEIATVGLSLE
CCEEEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCHHHCCCCCCEEECCCHH
EATKRGYKAKLASFPFQALGKSQAALQAEGFAQIVVEEKSGQILGAQVVGYEASVMIAEM
HHHHCCCHHHHHCCCHHHHCCCHHHHHHCCEEEEEEECCCCCEEEEEEECCCCEEHHHHH
TLAIANELTVECVAETIHAHPTLPEAWLESALLGEGLPLHLPPKTQKKG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]