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The map label for this gene is ilvE

Identifier: 45659307

GI number: 45659307

Start: 4275118

End: 4276074

Strand: Direct

Name: ilvE

Synonym: LIC13496

Alternate gene names: 45659307

Gene position: 4275118-4276074 (Clockwise)

Preceding gene: 45659305

Following gene: 45659308

Centisome position: 99.95

GC content: 36.68

Gene sequence:

>957_bases
ATGGAATTATATCACTTATCAGGTTCCTGTTCTATGGCAGAATCCATCAAGAAACTATCCTATTTCGAAGGAAAAATCGT
TCCCGAGTCCGAAGCGAAAATTAGCATTCAAACTCATGCGCTTCAATATGGAACAACCGTCTTTGGTGGGCTTCGCGGCT
ACTACGATAAAGACACGGATAATATTTATCTTTTTCGTATCTTAGATCATTATCAAAGATTAATCAACTCAACTCGAATC
ATGCAGCTGAAATTAGATAAGATAAAAGAAGAGTTGAGAGACATAACGATTGATTTGATCCGCCAGTGTGGCTACAAAGA
AAATATTTATCTTCGTCCCTTCGTTTATACATCGGCACTGCAGCTTTCACCTCGTTTTCATGATGTTCCGACACAACTCG
CGATCTACATTCTTCAGTTGAACGACTACTTAGATACAAAACGAGGATTAAAAACGATGGTATCTAGTTGGAGAAGATTT
GACGACGCAGTAATCCCAACTCTTTCCAAAGTATCCGGAGGATATGTAAATTCAGCTCTTGCAAAATCGGAAGCAGTTCA
AAATGGATTTGATGAAGCAATTTTTTTAGATTCGAGAGGATTTGTAAGCGAAGGTTCTGCTGAAAATATTTTTTTAGTTC
GAGATGGGAAGATCATTACACCCGGAATCAACTCCTCTTTATTAGAAGGAATTACTCGAAGAAGTGTACTTCAAATCGCA
AAAGATAATGGGATCGAAGTAATCGAAAGAGATATATCCAGAAGCGAACTTTATATTGCTGACGAAGTTTTCTTTTCTGG
AACTGGGGTTCAAATTGCTTGGGTTTCAGAAATCGATCATAGAAAAATCGGAAATTCAGCTATTGGCCCAATTACTCAAA
AGATTCAAAACTTATTTTTTAATCTAGTGATTAACAAAGAAGAAAAATACAGACATTGGTTAACTCCAGTATATTAG

Upstream 100 bases:

>100_bases
CTTCCTGAAAATTAATTATAAATTTTAGAATGAGATGTTTCTCAAGGTTTGAGTCTTACGATTGCAAGATGAATCGACAA
GAGACCCAACATATTGATTC

Downstream 100 bases:

>100_bases
AAAAGAATGAAATCGTTTCAGTTGGATGAAATTTTAGGACAAGAAGTTGCTCTTACTTTTTTAAAAAGATATATTTCCAA
ACCAGAAACCATTCCACCAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MELYHLSGSCSMAESIKKLSYFEGKIVPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRI
MQLKLDKIKEELRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTQLAIYILQLNDYLDTKRGLKTMVSSWRRF
DDAVIPTLSKVSGGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIA
KDNGIEVIERDISRSELYIADEVFFSGTGVQIAWVSEIDHRKIGNSAIGPITQKIQNLFFNLVINKEEKYRHWLTPVY

Sequences:

>Translated_318_residues
MELYHLSGSCSMAESIKKLSYFEGKIVPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRI
MQLKLDKIKEELRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTQLAIYILQLNDYLDTKRGLKTMVSSWRRF
DDAVIPTLSKVSGGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIA
KDNGIEVIERDISRSELYIADEVFFSGTGVQIAWVSEIDHRKIGNSAIGPITQKIQNLFFNLVINKEEKYRHWLTPVY
>Mature_318_residues
MELYHLSGSCSMAESIKKLSYFEGKIVPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRI
MQLKLDKIKEELRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTQLAIYILQLNDYLDTKRGLKTMVSSWRRF
DDAVIPTLSKVSGGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIA
KDNGIEVIERDISRSELYIADEVFFSGTGVQIAWVSEIDHRKIGNSAIGPITQKIQNLFFNLVINKEEKYRHWLTPVY

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=302, Percent_Identity=38.0794701986755, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 36273; Mature: 36273

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYHLSGSCSMAESIKKLSYFEGKIVPESEAKISIQTHALQYGTTVFGGLRGYYDKDTD
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCHHHHHHHCCCCCCCCC
NIYLFRILDHYQRLINSTRIMQLKLDKIKEELRDITIDLIRQCGYKENIYLRPFVYTSAL
CEEEEHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHEEHHH
QLSPRFHDVPTQLAIYILQLNDYLDTKRGLKTMVSSWRRFDDAVIPTLSKVSGGYVNSAL
CCCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIA
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHH
KDNGIEVIERDISRSELYIADEVFFSGTGVQIAWVSEIDHRKIGNSAIGPITQKIQNLFF
HCCCHHHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
NLVINKEEKYRHWLTPVY
HEEECCCHHHHHCCCCCC
>Mature Secondary Structure
MELYHLSGSCSMAESIKKLSYFEGKIVPESEAKISIQTHALQYGTTVFGGLRGYYDKDTD
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCHHHHHHHCCCCCCCCC
NIYLFRILDHYQRLINSTRIMQLKLDKIKEELRDITIDLIRQCGYKENIYLRPFVYTSAL
CEEEEHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHEEHHH
QLSPRFHDVPTQLAIYILQLNDYLDTKRGLKTMVSSWRRFDDAVIPTLSKVSGGYVNSAL
CCCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIA
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHH
KDNGIEVIERDISRSELYIADEVFFSGTGVQIAWVSEIDHRKIGNSAIGPITQKIQNLFF
HCCCHHHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
NLVINKEEKYRHWLTPVY
HEEECCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]