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The map label for this gene is hycG

Identifier: 45659241

GI number: 45659241

Start: 4200452

End: 4201273

Strand: Direct

Name: hycG

Synonym: LIC13424

Alternate gene names: 45659241

Gene position: 4200452-4201273 (Clockwise)

Preceding gene: 45659240

Following gene: 45659242

Centisome position: 98.21

GC content: 39.66

Gene sequence:

>822_bases
ATGAAAAGTATATTCGAAATATTGAATATTATTCGTCCCGCAAAGACGATGAACTATAAAAAAGTATCCCCTCTAAATCC
AAATGCGAGAGGAATTCCTATTCCGGTTTTATCTTCAAACGAATCTTGCCTAACTTGCAAATCCTGCGAACAGGTCTGTC
CAACTCATTCCCTCAAGATTATATCTAAAGATAAGATGAGTTTTGATTACGGTGCTTGCCTTCAATGTGGAAAATGTTCC
GAGGTATGTTCTAACGGAAAGATTATAGATTCCGGTTTTGTTCATGTATATTCGACCGATCGTGAAACGTTACAAGTAGT
ATATACAAACGGAATGCCAGATGAAAAACCGGAAGTGGAAACGGAAGAAATAAAACAATTCAGAAAGGTTACTAAAAAAA
CCGGCTTTCAATTTAGAGAAGTAGCGGCTAGTGGAAATAATACGACCGAAGCGGAAATTAATGCAAGTTTCAACGCTCTT
TTTGACAGTGAGGCAAGTAAGATCCGAGTAGTAGCTTCTCCAAAACACGCAGACGCACTGGTATATTCAGGGCCGGTAGG
GCCAAACATGGAAGGTCCCTTAGACACAGCCTGGGAAACTATGCCTTCTCCAAAAGCACTTGTAGCCTGCGGATCGGAAG
CCGTTTCGGGTGGTCTTTTTAAATTAGGTAAACTTCCAAAACAACCGGATTTATTTATTGGAGGTGATCCTCCTCGTCCT
GATGTAATCGTTTCTGCGTTTCGTTATCTAATGGGAATCTGGGAATTTTCTTTTAGAGCAGAACTTTCAAAACACGTTCA
AAATTTAAGGAATACTAAATAG

Upstream 100 bases:

>100_bases
CCAATTGGCACGCATTAGAATTAGCGGTACGAGGGGAATATATAGGGGACTTTCCACTAAATAACAAATCCTTCAATCTG
AGTTACGTAGGAGTTGATTT

Downstream 100 bases:

>100_bases
TCTAACAACAATTTGGCGTAAAAATCAGAAAAAACCGTCTTTTAAATAAATTGATGGGAATTTCCACGAAATCGTGTCAG
TTTTATACTGGGAGATTTTC

Product: hydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKSIFEILNIIRPAKTMNYKKVSPLNPNARGIPIPVLSSNESCLTCKSCEQVCPTHSLKIISKDKMSFDYGACLQCGKCS
EVCSNGKIIDSGFVHVYSTDRETLQVVYTNGMPDEKPEVETEEIKQFRKVTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEGPLDTAWETMPSPKALVACGSEAVSGGLFKLGKLPKQPDLFIGGDPPRP
DVIVSAFRYLMGIWEFSFRAELSKHVQNLRNTK

Sequences:

>Translated_273_residues
MKSIFEILNIIRPAKTMNYKKVSPLNPNARGIPIPVLSSNESCLTCKSCEQVCPTHSLKIISKDKMSFDYGACLQCGKCS
EVCSNGKIIDSGFVHVYSTDRETLQVVYTNGMPDEKPEVETEEIKQFRKVTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEGPLDTAWETMPSPKALVACGSEAVSGGLFKLGKLPKQPDLFIGGDPPRP
DVIVSAFRYLMGIWEFSFRAELSKHVQNLRNTK
>Mature_273_residues
MKSIFEILNIIRPAKTMNYKKVSPLNPNARGIPIPVLSSNESCLTCKSCEQVCPTHSLKIISKDKMSFDYGACLQCGKCS
EVCSNGKIIDSGFVHVYSTDRETLQVVYTNGMPDEKPEVETEEIKQFRKVTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEGPLDTAWETMPSPKALVACGSEAVSGGLFKLGKLPKQPDLFIGGDPPRP
DVIVSAFRYLMGIWEFSFRAELSKHVQNLRNTK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789074, Length=164, Percent_Identity=33.5365853658537, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788834, Length=149, Percent_Identity=31.5436241610738, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 29976; Mature: 29976

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIFEILNIIRPAKTMNYKKVSPLNPNARGIPIPVLSSNESCLTCKSCEQVCPTHSLKI
CHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHCCCCCEEE
ISKDKMSFDYGACLQCGKCSEVCSNGKIIDSGFVHVYSTDRETLQVVYTNGMPDEKPEVE
EECCCCCCCHHHHHCCCCHHHHHCCCCEEECCEEEEEECCCCEEEEEEECCCCCCCCCCC
TEEIKQFRKVTKKTGFQFREVAASGNNTTEAEINASFNALFDSEASKIRVVASPKHADAL
HHHHHHHHHHHHHCCCCEEHHHHCCCCCCEEEECCCHHHEECCCCCEEEEEECCCCCCEE
VYSGPVGPNMEGPLDTAWETMPSPKALVACGSEAVSGGLFKLGKLPKQPDLFIGGDPPRP
EEECCCCCCCCCCCCHHHHCCCCCHHEEEECCHHHCCCHHHHCCCCCCCCEEECCCCCCH
DVIVSAFRYLMGIWEFSFRAELSKHVQNLRNTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSIFEILNIIRPAKTMNYKKVSPLNPNARGIPIPVLSSNESCLTCKSCEQVCPTHSLKI
CHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHCCCCCEEE
ISKDKMSFDYGACLQCGKCSEVCSNGKIIDSGFVHVYSTDRETLQVVYTNGMPDEKPEVE
EECCCCCCCHHHHHCCCCHHHHHCCCCEEECCEEEEEECCCCEEEEEEECCCCCCCCCCC
TEEIKQFRKVTKKTGFQFREVAASGNNTTEAEINASFNALFDSEASKIRVVASPKHADAL
HHHHHHHHHHHHHCCCCEEHHHHCCCCCCEEEECCCHHHEECCCCCEEEEEECCCCCCEE
VYSGPVGPNMEGPLDTAWETMPSPKALVACGSEAVSGGLFKLGKLPKQPDLFIGGDPPRP
EEECCCCCCCCCCCCHHHHCCCCCHHEEEECCHHHCCCHHHHCCCCCCCCEEECCCCCCH
DVIVSAFRYLMGIWEFSFRAELSKHVQNLRNTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]