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The map label for this gene is gyrA [H]

Identifier: 45659166

GI number: 45659166

Start: 4103363

End: 4104751

Strand: Direct

Name: gyrA [H]

Synonym: LIC13347

Alternate gene names: 45659166

Gene position: 4103363-4104751 (Clockwise)

Preceding gene: 45659155

Following gene: 45659167

Centisome position: 95.94

GC content: 37.01

Gene sequence:

>1389_bases
ATGAAGAATTCCAATCCACCAAAATCAAAAGAATCGTTTCCAAAACGTCCTTTTGAAGATCAAGTTAATGACGATCAAAG
AAAATATTCTCGTTATGTATGCGATTCTCGGGCGATTCCGCACGAAATCGACGGTCTGAAACCAGTTCAGAGAAGAATTC
TCTGGGCAATGTGGAACTCAGACGCAAGAAACCGTTATACAAAAACCGTAAAAGTTGCAGGGCTCGCAATGGGATACCAT
CCTCACGGTGATAAATCCATTCAAGACGCACTTTCTCAGATGGCACAAGACTTCACTTTTGCAAATAACATTCCTCTCGT
TTCAGGCGAAGGAACTTTCGGAGACGTCTTAGATCCAAGTGCAATCGCGTCCCCTCGTTATACAGAAGTAAAACTTTCGG
ACTTTGTAAAAGATTTAGGATTCTTCGAAAGTTTACCAGATATTGATTACGTTAAAAACTACGACGAAACAGAAGATGAA
CCAATTCATTTTGTTGGAAAAGTCCCAATCGTTCTTCTCAATAATATTCAAGGAATTGCAACTGGTTTTCGTTGTTTTAT
TCCGGGTCACAGGCTTGCAGATATTGTTAAGTCTCAAATCAATTATCTCAAAAGCAAAAAACCTCTTTCTTTAAAACCTT
GGTACAAAGATTTCAACGGCGAAGTAAAAATGGCAGAAACCGAAACGGGTAATATCACCATGTCCACTACTTTCGCGTTC
AAATGGGAAGGAGATACGCTTTATCTTACCGATTCTCCTATGAACTGGAACCGAGAAAAAGTTATCTCCTTACTTGATGA
TATTTTAGAGAGAAAAGATTCCTGGTTAAAGGACTACGTGGATTATTCCAGCCAGACTTTCCGAGTTGAACTTCAATATA
AAAAAGGCGAAAAACCGAGCCAAAAAGAAATCATGGCTCTTTTCAATAAAGAGGACGTTCAAACTCTTGCGATGAACGTA
ATCACTTACGACGGTAAGTTAAAAAATTTCAAACCGGAAGAAATCATCAAACGTTTCTGTGACTTCCGTAAAACACATCT
TATACGAAGATTCAAACGTCTTTCCGGTTTAGAAGAAGAAAAAATTGAAAGAAACTCGGAACTGATTCGTTTTATTAAAG
AGAAATGGAACGAAAAAGTAATTGGGATTAAATCCAAAAAAGATTTTGAAGAAAAACTCAAAACTTCTAAATTCAAATAC
TTTGAATGGTTATCTACTATTCCAGTGTATAGAATGACTATCGAAGAAGTCAAAAAATGCGAAGAAGCAATCGTAGAAGC
GAAAACTACTCTGGCTCGTTATCAAGGATTGGTCAAAGAAGATAAAAAATTGACCGAGTTTATGATCATAGAGCTGGAAG
AACTTCAAAACAAATGGGATAAAGTATGA

Upstream 100 bases:

>100_bases
GAATTGTTCAAATATTCGTTTCAAAAATACTGTAATTAAATCCGGTCGATTTTTTATTGTTGACAGGATAGTCTTGTAGA
ATTTCGTTGGCCAAAACCGT

Downstream 100 bases:

>100_bases
GCACTGACAAAACCAAAGTAAAAGAAAAATCATCCCAAGAAAGAAATTTTAAAAAGTTATCTAACGTAGAACACGTTCGA
ATGAGAACCGGTATGTGGTT

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKNSNPPKSKESFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKSKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDTLYLTDSPMNWNREKVISLLDDILERKDSWLKDYVDYSSQTFRVELQYKKGEKPSQKEIMALFNKEDVQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEEKLKTSKFKY
FEWLSTIPVYRMTIEEVKKCEEAIVEAKTTLARYQGLVKEDKKLTEFMIIELEELQNKWDKV

Sequences:

>Translated_462_residues
MKNSNPPKSKESFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKSKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDTLYLTDSPMNWNREKVISLLDDILERKDSWLKDYVDYSSQTFRVELQYKKGEKPSQKEIMALFNKEDVQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEEKLKTSKFKY
FEWLSTIPVYRMTIEEVKKCEEAIVEAKTTLARYQGLVKEDKKLTEFMIIELEELQNKWDKV
>Mature_462_residues
MKNSNPPKSKESFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKSKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDTLYLTDSPMNWNREKVISLLDDILERKDSWLKDYVDYSSQTFRVELQYKKGEKPSQKEIMALFNKEDVQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEEKLKTSKFKY
FEWLSTIPVYRMTIEEVKKCEEAIVEAKTTLARYQGLVKEDKKLTEFMIIELEELQNKWDKV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=374, Percent_Identity=28.3422459893048, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI19913406, Length=364, Percent_Identity=28.2967032967033, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789396, Length=473, Percent_Identity=26.4270613107822, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788562, Length=360, Percent_Identity=27.2222222222222, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=379, Percent_Identity=29.287598944591, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=370, Percent_Identity=28.1081081081081, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=434, Percent_Identity=27.4193548387097, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI115534553, Length=277, Percent_Identity=28.5198555956679, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=373, Percent_Identity=24.1286863270777, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136538, Length=365, Percent_Identity=26.5753424657534, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 54078; Mature: 54078

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSNPPKSKESFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
DARNRYTKTVKVAGLAMGYHPHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPS
CHHHHHHHHEEEEHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCH
AIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPIVLLNNIQGIA
HCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCEEEECCCHHHH
TGFRCFIPGHRLADIVKSQINYLKSKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCCCEEEEEEEEE
KWEGDTLYLTDSPMNWNREKVISLLDDILERKDSWLKDYVDYSSQTFRVELQYKKGEKPS
EECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
QKEIMALFNKEDVQTLAMNVITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEE
HHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIERNSELIRFIKEKWNEKVIGIKSKKDFEEKLKTSKFKYFEWLSTIPVYRMTIEEVKKC
HHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EEAIVEAKTTLARYQGLVKEDKKLTEFMIIELEELQNKWDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNSNPPKSKESFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
DARNRYTKTVKVAGLAMGYHPHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPS
CHHHHHHHHEEEEHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCH
AIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPIVLLNNIQGIA
HCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCEEEECCCHHHH
TGFRCFIPGHRLADIVKSQINYLKSKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCCCEEEEEEEEE
KWEGDTLYLTDSPMNWNREKVISLLDDILERKDSWLKDYVDYSSQTFRVELQYKKGEKPS
EECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
QKEIMALFNKEDVQTLAMNVITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEE
HHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIERNSELIRFIKEKWNEKVIGIKSKKDFEEKLKTSKFKYFEWLSTIPVYRMTIEEVKKC
HHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EEAIVEAKTTLARYQGLVKEDKKLTEFMIIELEELQNKWDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8384814; 10688204 [H]