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The map label for this gene is htrB

Identifier: 45659044

GI number: 45659044

Start: 3954997

End: 3955968

Strand: Direct

Name: htrB

Synonym: LIC13222

Alternate gene names: 45659044

Gene position: 3954997-3955968 (Clockwise)

Preceding gene: 45659038

Following gene: 45659049

Centisome position: 92.47

GC content: 36.52

Gene sequence:

>972_bases
TTGTTCTTTTGCTTGTCAATCCGGATAGAAACCAATCCATTTGTAAGAAAAGAAAATTTCGGGGTCCTCGTGATTCGTTA
TATACCTGCTTTTATCTTTGTTTATTTATTTTATCTTCCGTTTCGAATTTTACCGTATCGTTTGTGTCTTTTATACGGAA
AATTTCTTGTGATTCTCCTTTATCCGTTTGCAAGAAAACATAGAAGGATTGCATACGAAAATATCTCCCACGCTTTTCCG
GAATATACAGAATCACAAAAGAAAGAACTTGTTTGGAAAAGTGTTCTTCATATCGGGAATTTAGTCGCAGGCACTTTATT
TGCTCCTCGTTTAAATCAAAAATGGATGGATCGTTATTTAGTTTACGATCCGAAATCTTTGGAGATCGAAAAGAAAACAA
ACGAAGAAGGTGTAGGAGTCGTTTTAATTTCCGGTCATTTTGGAACTTGGGAAATTTTAGTTCAGTTTATGGGAGTCCGT
ATGAAAGGCGGAGGGATTTATAAAAAAGTCAGAAATCCTCTCGTGGATAAACTAATTTATAAACTTAGAACAAAAAACGG
AATCAAACTCGTTTCCACAGAAGAATCCAGTCAAGTGACTAAAATGTTAAAACAAGGTTATTGGATTGGTTTTGGTTCAG
ATCAAAATGCGGGCAAAGTCGGAATTTTCGTAAACTTTTTCAATCGCCCTGCATCTACCTATCAAGGCCCCGCTTTAATG
GCTTATCTTACGGGTGCAAAGATGCTTTTGTATTCGGTTCTTTGCGGAGAGAAAGGTAAAGTGATCGTTAGAGTTAAAGA
CTTAGGTTTTGTGGATAAAAAAGCGTTTACTGATAGAGAAACCGCAATTCGTCACTATACAGAAGTTTGGACAAAAGCCT
TAGAAGAAGAAGTGAAACTTTTTCCAGAACAATACTTTTGGGTCCATAGACGCTGGAGAACAAAACCAGGGGATTTTCCT
GGTCAAATTTAA

Upstream 100 bases:

>100_bases
ATTAATTTTTATATCAGTAATAAACTTGCAAAGTTAGTTATATGTATCTTGATTCACTAAAAAATGTAGGAACTCATACA
TTGAGTTCAAAAAAATTTGC

Downstream 100 bases:

>100_bases
CTCATTATTTATAATTTTATAATATTCTAATTTTTATCCAAATTAATTACTTAGATGAACTTTTTTATATAGAACCATCT
CAAAAATATCTTAGAATAAT

Product: lauroyl/myristoyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFFCLSIRIETNPFVRKENFGVLVIRYIPAFIFVYLFYLPFRILPYRLCLLYGKFLVILLYPFARKHRRIAYENISHAFP
EYTESQKKELVWKSVLHIGNLVAGTLFAPRLNQKWMDRYLVYDPKSLEIEKKTNEEGVGVVLISGHFGTWEILVQFMGVR
MKGGGIYKKVRNPLVDKLIYKLRTKNGIKLVSTEESSQVTKMLKQGYWIGFGSDQNAGKVGIFVNFFNRPASTYQGPALM
AYLTGAKMLLYSVLCGEKGKVIVRVKDLGFVDKKAFTDRETAIRHYTEVWTKALEEEVKLFPEQYFWVHRRWRTKPGDFP
GQI

Sequences:

>Translated_323_residues
MFFCLSIRIETNPFVRKENFGVLVIRYIPAFIFVYLFYLPFRILPYRLCLLYGKFLVILLYPFARKHRRIAYENISHAFP
EYTESQKKELVWKSVLHIGNLVAGTLFAPRLNQKWMDRYLVYDPKSLEIEKKTNEEGVGVVLISGHFGTWEILVQFMGVR
MKGGGIYKKVRNPLVDKLIYKLRTKNGIKLVSTEESSQVTKMLKQGYWIGFGSDQNAGKVGIFVNFFNRPASTYQGPALM
AYLTGAKMLLYSVLCGEKGKVIVRVKDLGFVDKKAFTDRETAIRHYTEVWTKALEEEVKLFPEQYFWVHRRWRTKPGDFP
GQI
>Mature_323_residues
MFFCLSIRIETNPFVRKENFGVLVIRYIPAFIFVYLFYLPFRILPYRLCLLYGKFLVILLYPFARKHRRIAYENISHAFP
EYTESQKKELVWKSVLHIGNLVAGTLFAPRLNQKWMDRYLVYDPKSLEIEKKTNEEGVGVVLISGHFGTWEILVQFMGVR
MKGGGIYKKVRNPLVDKLIYKLRTKNGIKLVSTEESSQVTKMLKQGYWIGFGSDQNAGKVGIFVNFFNRPASTYQGPALM
AYLTGAKMLLYSVLCGEKGKVIVRVKDLGFVDKKAFTDRETAIRHYTEVWTKALEEEVKLFPEQYFWVHRRWRTKPGDFP
GQI

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=284, Percent_Identity=26.056338028169, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082094, Length=282, Percent_Identity=23.0496453900709, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37676; Mature: 37676

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFCLSIRIETNPFVRKENFGVLVIRYIPAFIFVYLFYLPFRILPYRLCLLYGKFLVILL
CEEEEEEEECCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPFARKHRRIAYENISHAFPEYTESQKKELVWKSVLHIGNLVAGTLFAPRLNQKWMDRYL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEE
VYDPKSLEIEKKTNEEGVGVVLISGHFGTWEILVQFMGVRMKGGGIYKKVRNPLVDKLIY
EECCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHH
KLRTKNGIKLVSTEESSQVTKMLKQGYWIGFGSDQNAGKVGIFVNFFNRPASTYQGPALM
HHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHH
AYLTGAKMLLYSVLCGEKGKVIVRVKDLGFVDKKAFTDRETAIRHYTEVWTKALEEEVKL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FPEQYFWVHRRWRTKPGDFPGQI
CHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFFCLSIRIETNPFVRKENFGVLVIRYIPAFIFVYLFYLPFRILPYRLCLLYGKFLVILL
CEEEEEEEECCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPFARKHRRIAYENISHAFPEYTESQKKELVWKSVLHIGNLVAGTLFAPRLNQKWMDRYL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEE
VYDPKSLEIEKKTNEEGVGVVLISGHFGTWEILVQFMGVRMKGGGIYKKVRNPLVDKLIY
EECCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHH
KLRTKNGIKLVSTEESSQVTKMLKQGYWIGFGSDQNAGKVGIFVNFFNRPASTYQGPALM
HHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHH
AYLTGAKMLLYSVLCGEKGKVIVRVKDLGFVDKKAFTDRETAIRHYTEVWTKALEEEVKL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FPEQYFWVHRRWRTKPGDFPGQI
CHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]