Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is metG

Identifier: 45658951

GI number: 45658951

Start: 3829833

End: 3831980

Strand: Direct

Name: metG

Synonym: LIC13129

Alternate gene names: 45658951

Gene position: 3829833-3831980 (Clockwise)

Preceding gene: 45658948

Following gene: 45658952

Centisome position: 89.54

GC content: 36.59

Gene sequence:

>2148_bases
GTGAACTCTTCTTCATCTCATAGAAATATACTAGTTACTTCGGCTCTTCCTTATGCAAACGGTCCGATTCATTTAGGTCA
CGTATTAGAAGGAATTCAAACAGACATTTGGGTTCGATTTCAAAAGGCAATTGGAAACGAATGTTATTTTTTCTGTGCAG
ACGACACGCATGGAACCCCCGTAATGTTAGCGGCGCGTAAGGAAGGGATTACTCCCGAACAACTTATCGAAAGAGTAGGA
CAAGAACATTATAGAGATCTAACTTCTTTCGGAATTGAATATGATCATTATGATTCCACACATTCGAAAGCGAATCAAGA
AATTTCCAAAGATATTTATCTGAAACTAAAATCCAAAGGTCATATCTCGAGAAGAAGTATAGAACAGTCATACTGCGAAA
CAGATAAAATGTTTCTTCCAGATCGTTTTATCAAAGGCACTTGCCCCAATTGTAAATCCAAAGATCAATATGGAGATAAT
TGCGAAGTTTGCGGCGCCACTTATAGCCCTAAAGATTTGATCGATTCTCACTGCTCTTTATGCGGAACTTCTCCAGTGGT
TAAAAACTCGGATCATATATTTTTTAAATTGGGAGATTTTCATAAAAAAGACGAAAAATCGACTTCATTGGAAACGATCA
ATCCATCCCATCTAAAAACTGATTTTGATTTACAATCCTGGATAGAAACGAGCGGGGTTGTTTCCGAATCAGAAGGTGTT
AAAAAGAAACTGAAAGAATGGTTCGATGCAGGACTACAAGATTGGGATATTTCCAGAGACGGCCCTTATTTCGGTTTTGA
AATCCCAGATGAAACAAATAAATATTTTTACGTTTGGTTAGATGCTCCTATAGGTTATATGGCTTCTTCCAAAAACTTTT
TTGAAAAGAATTTTCCAAACGAACCGAACAAGTTTGATTCCTTTTGGAAAAACAAAAACTCAGAGATCGTTCACTTTATC
GGTAAGGATATATTATACTTTCATACTTTATTTTGGCCAGCTATGTTAGAAGGTAGCGATTATAGAGCTCCTTCTAAAGT
TCACGTTCATGGTTTTATCGGTGTCAACGGTGAAAAAATGTCCAAATCAAGAGGAACGTTTATCAAAGCAGAAACGTTTG
TAAAATATCTAGACCCTGAACATCTTCGTTTTTATTTAGCCTCTAAATTAGGCCCTGGAATGGATGACATTGACCTTTCC
TTTGAAGACTTCATTAACAAGGTGAATTCCGATCTCGTAGGGAATTTGATCAATTCCGTTTCCAGGGTTTCTACTACTAT
TTTAGACGCATTAGACAGAACATTAGGAGTCGTTTCCAAAGAAGGTCTTGCTCTAATAGAAGAAATCTTATATCAAACTG
TAAAATTCGGTCCAGGCGAAGACTCAATTCAAAATATTATAAAATACGCATACGATCAAAGAAATTACGCTAAAGTTTTG
AGAGAAATTACACGTCTTGGAGATAGGGTAAACCGTTATGTCAACGACAATGCTCCATGGAAACTCATCAAAGAAAATCC
GGAAAAAGCAAGAGAAGTTGTAACTGTAACCTTAAACGCAAGCCGATTTCTTGCGATTTACCTTTACCCTGTGGTTCCTA
AAATTTCAGAACAGATTTACAAGCTACTGAATTTACAGGATTCTCCTTCGTTTAAGGATTTAGATAAAAACAGAATATTA
GAAAATATCAAAGTATTACCTTACGAAATGATTTCTAAACGAGTAGATGAAAAGGCGATCAAAGTTATGTTAGAAGAAAA
TAAACAATCAGAACATTCTAAAAAAGTAGAAACTTCCGAAAATCCAGTTCCAGAAGAACGACTTGAAATTTCAATAGACG
ACCTTTCTAAAGTAGAATTGAGAGTGGGTCAAATTGTGGAAGCCGGCCCGGTAGACGGCGCAGATAAGCTCGTAAACGTA
AAAGTGGATCTAGGTGAACTAGGAATCAAAAATGTTTTTGCCGGAATTAAAGTCGCTTATCAACCGGAAAATTTAAAAGG
TCTAAAGGTTGTGGTCGTTGCCAATCTCAAACCTAGAAAAATGAAGTTCGGGATTTCCGAAGCGATGTTGCTCGCTTCCG
GTGAAGGTGAATCTTTAAGTTTATTTATACCTCATAAGGACGCTAAACCGGGAGATCGATTAAAATAA

Upstream 100 bases:

>100_bases
TCCAGGTTTTCAGAAAATTCTATCCGAACCATTGATTTTGCAATGAAAATCCGAAAAGAAAGACGTTGATTTTGACCTAA
ATTTTAAGAACCTGACTTTT

Downstream 100 bases:

>100_bases
ACGACTTGTTTGGAAATTGTATTAGAGTTGTTAGAAATTAATTCTCCATCTGTTTCCGTTGTATTAAAATGGATGATAGT
TGAAGTAGTTTTGCTAATTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MNSSSSHRNILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEGITPEQLIERVG
QEHYRDLTSFGIEYDHYDSTHSKANQEISKDIYLKLKSKGHISRRSIEQSYCETDKMFLPDRFIKGTCPNCKSKDQYGDN
CEVCGATYSPKDLIDSHCSLCGTSPVVKNSDHIFFKLGDFHKKDEKSTSLETINPSHLKTDFDLQSWIETSGVVSESEGV
KKKLKEWFDAGLQDWDISRDGPYFGFEIPDETNKYFYVWLDAPIGYMASSKNFFEKNFPNEPNKFDSFWKNKNSEIVHFI
GKDILYFHTLFWPAMLEGSDYRAPSKVHVHGFIGVNGEKMSKSRGTFIKAETFVKYLDPEHLRFYLASKLGPGMDDIDLS
FEDFINKVNSDLVGNLINSVSRVSTTILDALDRTLGVVSKEGLALIEEILYQTVKFGPGEDSIQNIIKYAYDQRNYAKVL
REITRLGDRVNRYVNDNAPWKLIKENPEKAREVVTVTLNASRFLAIYLYPVVPKISEQIYKLLNLQDSPSFKDLDKNRIL
ENIKVLPYEMISKRVDEKAIKVMLEENKQSEHSKKVETSENPVPEERLEISIDDLSKVELRVGQIVEAGPVDGADKLVNV
KVDLGELGIKNVFAGIKVAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFIPHKDAKPGDRLK

Sequences:

>Translated_715_residues
MNSSSSHRNILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEGITPEQLIERVG
QEHYRDLTSFGIEYDHYDSTHSKANQEISKDIYLKLKSKGHISRRSIEQSYCETDKMFLPDRFIKGTCPNCKSKDQYGDN
CEVCGATYSPKDLIDSHCSLCGTSPVVKNSDHIFFKLGDFHKKDEKSTSLETINPSHLKTDFDLQSWIETSGVVSESEGV
KKKLKEWFDAGLQDWDISRDGPYFGFEIPDETNKYFYVWLDAPIGYMASSKNFFEKNFPNEPNKFDSFWKNKNSEIVHFI
GKDILYFHTLFWPAMLEGSDYRAPSKVHVHGFIGVNGEKMSKSRGTFIKAETFVKYLDPEHLRFYLASKLGPGMDDIDLS
FEDFINKVNSDLVGNLINSVSRVSTTILDALDRTLGVVSKEGLALIEEILYQTVKFGPGEDSIQNIIKYAYDQRNYAKVL
REITRLGDRVNRYVNDNAPWKLIKENPEKAREVVTVTLNASRFLAIYLYPVVPKISEQIYKLLNLQDSPSFKDLDKNRIL
ENIKVLPYEMISKRVDEKAIKVMLEENKQSEHSKKVETSENPVPEERLEISIDDLSKVELRVGQIVEAGPVDGADKLVNV
KVDLGELGIKNVFAGIKVAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFIPHKDAKPGDRLK
>Mature_715_residues
MNSSSSHRNILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEGITPEQLIERVG
QEHYRDLTSFGIEYDHYDSTHSKANQEISKDIYLKLKSKGHISRRSIEQSYCETDKMFLPDRFIKGTCPNCKSKDQYGDN
CEVCGATYSPKDLIDSHCSLCGTSPVVKNSDHIFFKLGDFHKKDEKSTSLETINPSHLKTDFDLQSWIETSGVVSESEGV
KKKLKEWFDAGLQDWDISRDGPYFGFEIPDETNKYFYVWLDAPIGYMASSKNFFEKNFPNEPNKFDSFWKNKNSEIVHFI
GKDILYFHTLFWPAMLEGSDYRAPSKVHVHGFIGVNGEKMSKSRGTFIKAETFVKYLDPEHLRFYLASKLGPGMDDIDLS
FEDFINKVNSDLVGNLINSVSRVSTTILDALDRTLGVVSKEGLALIEEILYQTVKFGPGEDSIQNIIKYAYDQRNYAKVL
REITRLGDRVNRYVNDNAPWKLIKENPEKAREVVTVTLNASRFLAIYLYPVVPKISEQIYKLLNLQDSPSFKDLDKNRIL
ENIKVLPYEMISKRVDEKAIKVMLEENKQSEHSKKVETSENPVPEERLEISIDDLSKVELRVGQIVEAGPVDGADKLVNV
KVDLGELGIKNVFAGIKVAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFIPHKDAKPGDRLK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=551, Percent_Identity=27.4047186932849, Blast_Score=216, Evalue=7e-56,
Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=25.497287522604, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1788432, Length=715, Percent_Identity=45.5944055944056, Blast_Score=631, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=563, Percent_Identity=31.0834813499112, Blast_Score=263, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI212645096, Length=540, Percent_Identity=24.0740740740741, Blast_Score=152, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=25.9475218658892, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=594, Percent_Identity=30.8080808080808, Blast_Score=240, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6321610, Length=563, Percent_Identity=22.7353463587922, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=27.9857397504456, Blast_Score=225, Evalue=8e-59,
Organism=Drosophila melanogaster, GI21358483, Length=551, Percent_Identity=23.5934664246824, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24651924, Length=100, Percent_Identity=40, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_LEPIC (Q72MR4)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_003037.1
- ProteinModelPortal:   Q72MR4
- GeneID:   2772511
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC13129
- NMPDR:   fig|267671.1.peg.3037
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   LINT267671:LIC_13129-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 81100; Mature: 81100

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNSSSSHRNILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTP
CCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
VMLAARKEGITPEQLIERVGQEHYRDLTSFGIEYDHYDSTHSKANQEISKDIYLKLKSKG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEECCCC
HISRRSIEQSYCETDKMFLPDRFIKGTCPNCKSKDQYGDNCEVCGATYSPKDLIDSHCSL
CHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
CGTSPVVKNSDHIFFKLGDFHKKDEKSTSLETINPSHLKTDFDLQSWIETSGVVSESEGV
CCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCHHHH
KKKLKEWFDAGLQDWDISRDGPYFGFEIPDETNKYFYVWLDAPIGYMASSKNFFEKNFPN
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCHHCCCCHHHCCCCC
EPNKFDSFWKNKNSEIVHFIGKDILYFHTLFWPAMLEGSDYRAPSKVHVHGFIGVNGEKM
CCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHH
SKSRGTFIKAETFVKYLDPEHLRFYLASKLGPGMDDIDLSFEDFINKVNSDLVGNLINSV
HCCCCCEEEHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SRVSTTILDALDRTLGVVSKEGLALIEEILYQTVKFGPGEDSIQNIIKYAYDQRNYAKVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
REITRLGDRVNRYVNDNAPWKLIKENPEKAREVVTVTLNASRFLAIYLYPVVPKISEQIY
HHHHHHHHHHHHHCCCCCCEEEECCCHHHCEEEEEEEECCCEEEEEEEECCCHHHHHHHH
KLLNLQDSPSFKDLDKNRILENIKVLPYEMISKRVDEKAIKVMLEENKQSEHSKKVETSE
HHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
NPVPEERLEISIDDLSKVELRVGQIVEAGPVDGADKLVNVKVDLGELGIKNVFAGIKVAY
CCCCHHHEEEECCCHHHHHHHHHCEEECCCCCCCCCEEEEEEEHHHCCHHHHHHCCEEEE
QPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFIPHKDAKPGDRLK
CCCCCCCEEEEEEECCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNSSSSHRNILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTP
CCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
VMLAARKEGITPEQLIERVGQEHYRDLTSFGIEYDHYDSTHSKANQEISKDIYLKLKSKG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEECCCC
HISRRSIEQSYCETDKMFLPDRFIKGTCPNCKSKDQYGDNCEVCGATYSPKDLIDSHCSL
CHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
CGTSPVVKNSDHIFFKLGDFHKKDEKSTSLETINPSHLKTDFDLQSWIETSGVVSESEGV
CCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCHHHH
KKKLKEWFDAGLQDWDISRDGPYFGFEIPDETNKYFYVWLDAPIGYMASSKNFFEKNFPN
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCHHCCCCHHHCCCCC
EPNKFDSFWKNKNSEIVHFIGKDILYFHTLFWPAMLEGSDYRAPSKVHVHGFIGVNGEKM
CCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHH
SKSRGTFIKAETFVKYLDPEHLRFYLASKLGPGMDDIDLSFEDFINKVNSDLVGNLINSV
HCCCCCEEEHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SRVSTTILDALDRTLGVVSKEGLALIEEILYQTVKFGPGEDSIQNIIKYAYDQRNYAKVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
REITRLGDRVNRYVNDNAPWKLIKENPEKAREVVTVTLNASRFLAIYLYPVVPKISEQIY
HHHHHHHHHHHHHCCCCCCEEEECCCHHHCEEEEEEEECCCEEEEEEEECCCHHHHHHHH
KLLNLQDSPSFKDLDKNRILENIKVLPYEMISKRVDEKAIKVMLEENKQSEHSKKVETSE
HHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
NPVPEERLEISIDDLSKVELRVGQIVEAGPVDGADKLVNVKVDLGELGIKNVFAGIKVAY
CCCCHHHEEEECCCHHHHHHHHHCEEECCCCCCCCCEEEEEEEHHHCCHHHHHHCCEEEE
QPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFIPHKDAKPGDRLK
CCCCCCCEEEEEEECCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA