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The map label for this gene is ptsN

Identifier: 45658782

GI number: 45658782

Start: 3587895

End: 3588362

Strand: Reverse

Name: ptsN

Synonym: LIC12955

Alternate gene names: 45658782

Gene position: 3588362-3587895 (Counterclockwise)

Preceding gene: 45658783

Following gene: 45658781

Centisome position: 83.9

GC content: 36.11

Gene sequence:

>468_bases
ATGGATATGAATCAGCTCTTGACTCTTCTTCAACCAAAAACAGTTATTTTTAATTTAGAATCCGGAAGCAAAGAAGAAGT
GATTTCCAGACTTCTGCAAAAGGCGATAGATACACATCAGATAGATACGGAAAACAAAGAAGAAATTTTAGAGTCTCTTC
TTGCTAGAGAAAAGTCAATGTCTACCGGAATTGGAAGTGGAGTTGCGATTCCTCATTGTTCTGTCAATCTTGTAGATGAA
CTCAAATGTGTGATGGGGCTTAATCCAAATGGGATTGATTTTGATTCGATTGATCATCAACCGGTTCATATTTTTATTCT
TCTTATCGTTCCCAAAACCAAATTTCAAGAACATATCAAGACGCTCGCGCAGATTGCAAAAGCATTAAATGTAAAAGAAG
ATAGAGAAAAATTGATTCGTTCTGGATCCTTCGAGGAAATCCAAAAAGCTTTTTCTCGGAACGTCTAA

Upstream 100 bases:

>100_bases
AGAGTCAATAATTTCGGGCAATTATAGTAACTCGAATTGTAATTAGAATGATGATCGTGTATTGTAACAAACGTAGACAA
CAAAACGAAAAGTCAGGAGA

Downstream 100 bases:

>100_bases
CCGTGCAGGGAGAAATCTCTAGGTTCCTTTTATTTTTTGTCGCATTAATTTTTTTCTCCGTTCTTTTCGGTCATCTACGT
TCTTTAAATAAAGAATATCT

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MDMNQLLTLLQPKTVIFNLESGSKEEVISRLLQKAIDTHQIDTENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDE
LKCVMGLNPNGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV

Sequences:

>Translated_155_residues
MDMNQLLTLLQPKTVIFNLESGSKEEVISRLLQKAIDTHQIDTENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDE
LKCVMGLNPNGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
>Mature_155_residues
MDMNQLLTLLQPKTVIFNLESGSKEEVISRLLQKAIDTHQIDTENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDE
LKCVMGLNPNGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=105, Percent_Identity=39.0476190476191, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17405; Mature: 17405

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMNQLLTLLQPKTVIFNLESGSKEEVISRLLQKAIDTHQIDTENKEEILESLLAREKSM
CCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
STGIGSGVAIPHCSVNLVDELKCVMGLNPNGIDFDSIDHQPVHIFILLIVPKTKFQEHIK
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHH
TLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
HHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MDMNQLLTLLQPKTVIFNLESGSKEEVISRLLQKAIDTHQIDTENKEEILESLLAREKSM
CCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
STGIGSGVAIPHCSVNLVDELKCVMGLNPNGIDFDSIDHQPVHIFILLIVPKTKFQEHIK
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHH
TLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
HHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]