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The map label for this gene is tuf

Identifier: 45658705

GI number: 45658705

Start: 3469464

End: 3470669

Strand: Reverse

Name: tuf

Synonym: LIC12875

Alternate gene names: 45658705

Gene position: 3470669-3469464 (Counterclockwise)

Preceding gene: 45658706

Following gene: 45658704

Centisome position: 81.14

GC content: 43.7

Gene sequence:

>1206_bases
ATGGCTAAAGAAAAGTTTGATAGGTCTAAACCTCACTTAAACGTTGGAACAATTGGTCACGTAGATCACGGTAAAACAAC
CCTGACGGCAGCAATTACTACTACACTTGCAAAAGCGATCGGTGGTAAAAACAAAGCCGTTGCTTATGACCAAATTGATA
ACGCTCCGGAAGAAAAAGCTCGTGGAATCACCATTGCTACTTCCCACCAGGAATATGAAACTGCAAATCGTCACTACGCA
CACGTAGATTGTCCAGGTCACGCAGACTATGTAAAAAATATGATTACCGGTGCGGCTCAGATGGATGCGGCGATTCTTGT
AGTATCCGCAACTGACGGACCTATGCCACAAACAAAAGAACATATCCTTCTTGCTCGTCAGGTAGGTGTTCCATATGTAA
TTGTATTCATTAACAAAGCAGATATGCTTGCTGCTGACGAAAGAGCAGAAATGATCGAAATGGTTGAGATGGACGTTCGT
GAACTTCTCAATAAGTATAGCTTCCCAGGAGATACAACTCCTATCGTTCATGGTTCTGCGGTAAAAGCACTTGAGGGCGA
TGAATCTGAAATTGGGATGCCTGCAATTCTCAAATTGATGGAAGCTCTGGATACTTTCGTTCCAAATCCAAAACGTGTAA
TCGACAAACCTTTCCTTATGCCAGTAGAAGACGTTTTCTCGATCACTGGTCGTGGAACTGTTGCAACTGGAAGAGTGGAA
CAAGGTGTTTTGAAAGTGAACGACGAAGTTGAAATTATCGGTATCCGCCCAACAACAAAAACTGTTGTTACCGGTATCGA
AATGTTCAGAAAACTTCTCGATCAAGCGGAAGCTGGCGACAACATCGGCGCTCTTCTTCGTGGAACTAAAAAAGAAGAAA
TCGAAAGAGGGCAAGTTCTTGCGAAGCCAGGTTCTATCACTCCTCACAAAAAGTTTGCCGCTGAGGTGTATGTATTAACT
AAGGATGAAGGCGGACGTCATACTCCGTTTATCAATAACTACCGTCCTCAGTTTTACTTTAGAACAACTGACGTAACCGG
AGTTTGTAACCTTCCTAATGGTGTCGAAATGGTTATGCCTGGTGATAACGTTTCTTTGACGGTTGAATTGATTAGCCCGA
TCGCAATGGACAAGGGTCTTAAGTTCGCAATTCGCGAGGGTGGAAGAACCATTGGATCCGGCGTTGTCGCGGAAATCACT
GAGTAA

Upstream 100 bases:

>100_bases
TGTCTTTAGATTCCCTCTTTGACCCTGAACACTATTACGTAATTACATAAGTCGATTCCCGTTAGGGTTCGTTCAAAAGT
AAGGAGATTAAAAAGTCACT

Downstream 100 bases:

>100_bases
GAGTTTTTCTGAAAATGGCCGGACAAAAAATACGAGTTAAATTGAAGGCGTTCGATCATAGGTTGATTGATCAATCAACC
TATGAGATCGTAGCAACTGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETANRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVR
ELLNKYSFPGDTTPIVHGSAVKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVLAKPGSITPHKKFAAEVYVLT
KDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEIT
E

Sequences:

>Translated_401_residues
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETANRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVR
ELLNKYSFPGDTTPIVHGSAVKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVLAKPGSITPHKKFAAEVYVLT
KDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEIT
E
>Mature_400_residues
AKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETANRHYAH
VDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRE
LLNKYSFPGDTTPIVHGSAVKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVEQ
GVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVLAKPGSITPHKKFAAEVYVLTK
DEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEITE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.2025316455696, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI4503475, Length=352, Percent_Identity=32.3863636363636, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=29.5045045045045, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=29.5045045045045, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=352, Percent_Identity=32.6704545454545, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=30.188679245283, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=341, Percent_Identity=30.791788856305, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI194097354, Length=341, Percent_Identity=30.791788856305, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018520, Length=341, Percent_Identity=30.791788856305, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=31.3664596273292, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=25.7462686567164, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=31.7596566523605, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=31.7596566523605, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=72.75, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=400, Percent_Identity=72.75, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=367, Percent_Identity=29.700272479564, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=31.5018315018315, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=440, Percent_Identity=26.8181818181818, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.9850746268657, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=394, Percent_Identity=44.1624365482233, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=355, Percent_Identity=31.830985915493, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=355, Percent_Identity=31.830985915493, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=345, Percent_Identity=30.4347826086957, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=28.7671232876712, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.735632183908, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=28.9473684210526, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=278, Percent_Identity=30.2158273381295, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=176, Percent_Identity=32.3863636363636, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=242, Percent_Identity=28.9256198347107, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=278, Percent_Identity=26.6187050359712, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=434, Percent_Identity=23.5023041474654, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=62.3441396508728, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=353, Percent_Identity=32.5779036827195, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=353, Percent_Identity=32.5779036827195, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=25.0549450549451, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.8810408921933, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=276, Percent_Identity=28.2608695652174, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=149, Percent_Identity=33.5570469798658, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=55.8375634517767, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=55.8375634517767, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=48.0818414322251, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=28.0087527352298, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=28.0087527352298, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=32.8990228013029, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI45550900, Length=444, Percent_Identity=27.9279279279279, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=29.1907514450867, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24647046, Length=271, Percent_Identity=27.3062730627306, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24647048, Length=271, Percent_Identity=27.3062730627306, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43575; Mature: 43443

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKA
CCCCCCCCCCCCEECCCEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETANRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKE
CCEEEEECCHHHHHCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCHH
HILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRELLNKYSFPGDTTPIVHGSA
HEEEEHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCE
VKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCEEEECCHH
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEE
AKPGSITPHKKFAAEVYVLTKDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMP
ECCCCCCCCHHCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEC
GDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEITE
CCCCEEEEEECCCHHHCCCCEEEECCCCCCCCCCCEEEECC
>Mature Secondary Structure 
AKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKA
CCCCCCCCCCCEECCCEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETANRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKE
CCEEEEECCHHHHHCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCHH
HILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRELLNKYSFPGDTTPIVHGSA
HEEEEHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCE
VKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCEEEECCHH
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEE
AKPGSITPHKKFAAEVYVLTKDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMP
ECCCCCCCCHHCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEC
GDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEITE
CCCCEEEEEECCCHHHCCCCEEEECCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA