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The map label for this gene is ispD

Identifier: 45658454

GI number: 45658454

Start: 3166560

End: 3167276

Strand: Reverse

Name: ispD

Synonym: LIC12617

Alternate gene names: 45658454

Gene position: 3167276-3166560 (Counterclockwise)

Preceding gene: 45658455

Following gene: 45658453

Centisome position: 74.05

GC content: 36.82

Gene sequence:

>717_bases
ATGAAGTCACTGTTTCTCTCTGAAAAAATTTACGTTTTAATTTTAGCTGGTGGAACCGGTTCTAGAATGGGTTCTAAAAT
TCCAAAACAGTTTTTGGAATTAAATGGTGAGCCTATTCTTATTCATTCTTTAAAAAGATTTCAAAATTGGGGAAAACAAA
AACGAATCGTGCTGGTTTCCCATTTCGAATCGATCCCAAAAATTGAATCGATTTGTGCTTCTTATTTAGAAAATGAAGAC
AGGATCGTTCAAGGTGGGGAGAATAGACATTCTTCCATGTTATGTGGTTTGTCCGTTTTGGATTTTAAAGACGAAGATAT
TATTTTGGTTCACGACGCCGCCAGGCCTTTTGTTCTTGCGGATGAGTTGGATTCTCTTTGTGAAAAAGTGCGTTCCGACG
GGATTGCTACCTTGGCTTCTCGTACTTCTGAAACTGTATTAGAAGAATTGAATGGGAAAACCGTATCCTTTTTAGACCGC
GAGCACGTCTGGTTTATGAAAACTCCTCAGGGAATTCGTGGTGATGTTTTGAAAGAATTACTCACGTTTTCTGTGGATTC
GATTCCCACTGATCTTTGTTCTTGGGCTTTAACTTTTGGAAAAACTTCTTCCATTGTGGAATCTAATCCTTTAAATCTTA
AAATTACTCGTAAGGAAGATTTGGATCTTGCGGAAGTTTTTTCTTCTTTATTTCAAAAGATTTCATCGGACATATAA

Upstream 100 bases:

>100_bases
AATTACAACTCTTTTCCTGAAACGGCAGAGGTTTTGGTTGATTCGGATGGAAGTTTTCAACTAATTCGAAGACGTCAGAA
TTGGGAACAGATCTTTCAGA

Downstream 100 bases:

>100_bases
CTGCTCAGAATCATATAATAAAATACTTATTTAATAGTTTATGCTTTAATCCTATGAATTCATAGAAAGCGTTTTGCTGA
GTTCTTCAGTCGTTTCGCAT

Product: 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKSLFLSEKIYVLILAGGTGSRMGSKIPKQFLELNGEPILIHSLKRFQNWGKQKRIVLVSHFESIPKIESICASYLENED
RIVQGGENRHSSMLCGLSVLDFKDEDIILVHDAARPFVLADELDSLCEKVRSDGIATLASRTSETVLEELNGKTVSFLDR
EHVWFMKTPQGIRGDVLKELLTFSVDSIPTDLCSWALTFGKTSSIVESNPLNLKITRKEDLDLAEVFSSLFQKISSDI

Sequences:

>Translated_238_residues
MKSLFLSEKIYVLILAGGTGSRMGSKIPKQFLELNGEPILIHSLKRFQNWGKQKRIVLVSHFESIPKIESICASYLENED
RIVQGGENRHSSMLCGLSVLDFKDEDIILVHDAARPFVLADELDSLCEKVRSDGIATLASRTSETVLEELNGKTVSFLDR
EHVWFMKTPQGIRGDVLKELLTFSVDSIPTDLCSWALTFGKTSSIVESNPLNLKITRKEDLDLAEVFSSLFQKISSDI
>Mature_238_residues
MKSLFLSEKIYVLILAGGTGSRMGSKIPKQFLELNGEPILIHSLKRFQNWGKQKRIVLVSHFESIPKIESICASYLENED
RIVQGGENRHSSMLCGLSVLDFKDEDIILVHDAARPFVLADELDSLCEKVRSDGIATLASRTSETVLEELNGKTVSFLDR
EHVWFMKTPQGIRGDVLKELLTFSVDSIPTDLCSWALTFGKTSSIVESNPLNLKITRKEDLDLAEVFSSLFQKISSDI

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=243, Percent_Identity=27.5720164609054, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=31.7391304347826, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_LEPIC (Q72P59)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002540.1
- ProteinModelPortal:   Q72P59
- SMR:   Q72P59
- GeneID:   2772117
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12617
- HOGENOM:   HBG672839
- OMA:   REQQDFW
- ProtClustDB:   CLSK573749
- BioCyc:   LINT267671:LIC_12617-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26663; Mature: 26663

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFLSEKIYVLILAGGTGSRMGSKIPKQFLELNGEPILIHSLKRFQNWGKQKRIVLVS
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCEEEEEE
HFESIPKIESICASYLENEDRIVQGGENRHSSMLCGLSVLDFKDEDIILVHDAARPFVLA
HHHCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCEEECCCCCCEEEEECCCCCEEEH
DELDSLCEKVRSDGIATLASRTSETVLEELNGKTVSFLDREHVWFMKTPQGIRGDVLKEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHH
LTFSVDSIPTDLCSWALTFGKTSSIVESNPLNLKITRKEDLDLAEVFSSLFQKISSDI
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSLFLSEKIYVLILAGGTGSRMGSKIPKQFLELNGEPILIHSLKRFQNWGKQKRIVLVS
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCEEEEEE
HFESIPKIESICASYLENEDRIVQGGENRHSSMLCGLSVLDFKDEDIILVHDAARPFVLA
HHHCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCEEECCCCCCEEEEECCCCCEEEH
DELDSLCEKVRSDGIATLASRTSETVLEELNGKTVSFLDREHVWFMKTPQGIRGDVLKEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHH
LTFSVDSIPTDLCSWALTFGKTSSIVESNPLNLKITRKEDLDLAEVFSSLFQKISSDI
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA