Click here to switch to the map view.

The map label for this gene is yisK [H]

Identifier: 45658432

GI number: 45658432

Start: 3144882

End: 3145832

Strand: Direct

Name: yisK [H]

Synonym: LIC12595

Alternate gene names: 45658432

Gene position: 3144882-3145832 (Clockwise)

Preceding gene: 45658431

Following gene: 45658433

Centisome position: 73.53

GC content: 35.12

Gene sequence:

>951_bases
ATGGCAACTAACTATATACGGTTTAAAAAAAAGAATTCAATTCAATGGGGAAAAATCGAAGAGGATAAAATTCTAACTTT
GGACTGTGGTGATCTTTCTACGAAGGATTTTTTAGAGTTCCTAAAAAAAAAGAAAAAATATCCAAAACAAAAATTAATTC
CAATACAAGAAGTTTCTGTTCTATCTCCTATTACTTCTCCCTGTCAGATTATTTGCCAAGGCGCCAATTACAGACAACAT
CAGATCGAATCCGGTCTCGATCCGGACGATAAAACTTATAATTTATTTTTCACAAAATCGGACGCATCTCTTTCTCCTCC
AATCGGAAAAGTCATACGTCCTTCTCACGTAAAACTTCTAGATTATGAAATTGAACTCGGTTTTGTTTTTGGAAAATCTT
TCGATTCCACATTAAAACTAAATTCCAAAAGTATTAGAGAATATATCGCAGCTTTTTTTATGGCAAACGATATTTCCGCT
AGAGACGTTCAACTCCCTCAAATGCAATGGTACAAAGGTAAATCGTATCGAACCTTTTGCCCAACAGGTCCTTATCTTTC
AGTTTTAGATCCAGGAGATTTCGATCTTTTAGATTCTTTGGAGTTAACTCTTCTTGTAAACAGTCAGATCAGACAAAAAG
ATTCCGCTTCCAATCTAGTTTTTAAACCTTTAGAAAGCGTTTTAGAATTATCACAATTTTGTAATATATCTACTGGAGAC
GTATTATTAACCGGAACTCCCTCCGGATGTGCACTACGAGCTCCTGGAAAACTCATTCAAAAAATTGCAAGTATTTTACC
TGAAAAGGTAAAATGGAATCTTTTTATCAAAGGGCAAGAAAAAAGAACTCAGTATTTACGTCCTGGAGACGTGATCCGTT
CTACCATTCGTAGTGTAGATCATAGAATTGACCTGGGAGAACAAATTCTCGACATAGTTGAAGCAGATTAG

Upstream 100 bases:

>100_bases
TAACTAAATATGTTCTTTACCAATTGTTATATTTTTAAATTATATTAATCACAAATATGGCATTTTTATAAAACTCTCAC
TATACGTTTAGGTATTTTAT

Downstream 100 bases:

>100_bases
AAGTTATCTCAAAAAATATATTAGAACAATTGAAATCTGTGTTTTAAACAAAGATAAAGTATTTTTTAGATAGATTCCAT
GAATAACTATTAAACTCTAT

Product: fumarylacetoacetate hydrolase family protein

Products: acetoacetate; fumarate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MATNYIRFKKKNSIQWGKIEEDKILTLDCGDLSTKDFLEFLKKKKKYPKQKLIPIQEVSVLSPITSPCQIICQGANYRQH
QIESGLDPDDKTYNLFFTKSDASLSPPIGKVIRPSHVKLLDYEIELGFVFGKSFDSTLKLNSKSIREYIAAFFMANDISA
RDVQLPQMQWYKGKSYRTFCPTGPYLSVLDPGDFDLLDSLELTLLVNSQIRQKDSASNLVFKPLESVLELSQFCNISTGD
VLLTGTPSGCALRAPGKLIQKIASILPEKVKWNLFIKGQEKRTQYLRPGDVIRSTIRSVDHRIDLGEQILDIVEAD

Sequences:

>Translated_316_residues
MATNYIRFKKKNSIQWGKIEEDKILTLDCGDLSTKDFLEFLKKKKKYPKQKLIPIQEVSVLSPITSPCQIICQGANYRQH
QIESGLDPDDKTYNLFFTKSDASLSPPIGKVIRPSHVKLLDYEIELGFVFGKSFDSTLKLNSKSIREYIAAFFMANDISA
RDVQLPQMQWYKGKSYRTFCPTGPYLSVLDPGDFDLLDSLELTLLVNSQIRQKDSASNLVFKPLESVLELSQFCNISTGD
VLLTGTPSGCALRAPGKLIQKIASILPEKVKWNLFIKGQEKRTQYLRPGDVIRSTIRSVDHRIDLGEQILDIVEAD
>Mature_315_residues
ATNYIRFKKKNSIQWGKIEEDKILTLDCGDLSTKDFLEFLKKKKKYPKQKLIPIQEVSVLSPITSPCQIICQGANYRQHQ
IESGLDPDDKTYNLFFTKSDASLSPPIGKVIRPSHVKLLDYEIELGFVFGKSFDSTLKLNSKSIREYIAAFFMANDISAR
DVQLPQMQWYKGKSYRTFCPTGPYLSVLDPGDFDLLDSLELTLLVNSQIRQKDSASNLVFKPLESVLELSQFCNISTGDV
LLTGTPSGCALRAPGKLIQKIASILPEKVKWNLFIKGQEKRTQYLRPGDVIRSTIRSVDHRIDLGEQILDIVEAD

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=217, Percent_Identity=34.1013824884793, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI40786394, Length=217, Percent_Identity=33.6405529953917, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI215422413, Length=188, Percent_Identity=27.1276595744681, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI13654274, Length=188, Percent_Identity=27.1276595744681, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI66348062, Length=188, Percent_Identity=27.1276595744681, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787428, Length=189, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557057, Length=198, Percent_Identity=29.7979797979798, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324161, Length=223, Percent_Identity=26.9058295964126, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572127, Length=261, Percent_Identity=32.5670498084291, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24663695, Length=310, Percent_Identity=23.5483870967742, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571789, Length=173, Percent_Identity=28.3236994219653, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 3.7.1.2

Molecular weight: Translated: 35777; Mature: 35646

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNYIRFKKKNSIQWGKIEEDKILTLDCGDLSTKDFLEFLKKKKKYPKQKLIPIQEVSV
CCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHH
LSPITSPCQIICQGANYRQHQIESGLDPDDKTYNLFFTKSDASLSPPIGKVIRPSHVKLL
HCCCCCCHHHEECCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCCCEEEE
DYEIELGFVFGKSFDSTLKLNSKSIREYIAAFFMANDISARDVQLPQMQWYKGKSYRTFC
EEEEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEC
PTGPYLSVLDPGDFDLLDSLELTLLVNSQIRQKDSASNLVFKPLESVLELSQFCNISTGD
CCCCEEEEECCCCCCHHCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VLLTGTPSGCALRAPGKLIQKIASILPEKVKWNLFIKGQEKRTQYLRPGDVIRSTIRSVD
EEEEECCCCCEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCHHHHHHHHHHHH
HRIDLGEQILDIVEAD
HHCCHHHHHHHHHCCC
>Mature Secondary Structure 
ATNYIRFKKKNSIQWGKIEEDKILTLDCGDLSTKDFLEFLKKKKKYPKQKLIPIQEVSV
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHH
LSPITSPCQIICQGANYRQHQIESGLDPDDKTYNLFFTKSDASLSPPIGKVIRPSHVKLL
HCCCCCCHHHEECCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCCCEEEE
DYEIELGFVFGKSFDSTLKLNSKSIREYIAAFFMANDISARDVQLPQMQWYKGKSYRTFC
EEEEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEC
PTGPYLSVLDPGDFDLLDSLELTLLVNSQIRQKDSASNLVFKPLESVLELSQFCNISTGD
CCCCEEEEECCCCCCHHCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VLLTGTPSGCALRAPGKLIQKIASILPEKVKWNLFIKGQEKRTQYLRPGDVIRSTIRSVD
EEEEECCCCCEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCHHHHHHHHHHHH
HRIDLGEQILDIVEAD
HHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-fumarylacetoacetate; H2O

Specific reaction: 4-fumarylacetoacetate + H2O = acetoacetate + fumarate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]