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The map label for this gene is nylA

Identifier: 45658238

GI number: 45658238

Start: 2898330

End: 2899832

Strand: Reverse

Name: nylA

Synonym: LIC12392

Alternate gene names: 45658238

Gene position: 2899832-2898330 (Counterclockwise)

Preceding gene: 45658240

Following gene: 45658237

Centisome position: 67.8

GC content: 43.25

Gene sequence:

>1503_bases
TTGTATTCGGAGTCTCCTATGCCTCAACCTATTCCGGAATATACATATTACGATGCTATAGGACTGGCCACTCTTGTCCG
AAAAAAACTAATTCATCCTATTGAGCTCGTTGAATCTGCCATTCAAAGAATTGAAACCATAAATCCTGGGCTGAATGCAG
TAATTACCCGTTTTTACGAAGAAGCAAGAAAAACTTCAAAGTCTAAACTTCCGAACGGTCCTTTTCGGGGAGTTCCGATT
CTTGTAAAAGATATGGTTCATTCTATTGGAGGCGCTCCTCTGACTTGTGGTTCTAAAGCGTTTCGAAATTATATTTCGCC
CCAAGATTCTGAATTTATAAAAAGGCTTCGTAAAACAGGAACCATTTTTTTAGGACAAACGAATGTTCCCGAATTTGCTC
TTATGGGAATCACAGAACCGAAGTTATACGGTCCGACCCGAAATCCTTGGAACCTAGAAAGAACTCCGGGTGGTTCTTCT
GGTGGTTCTGCGGCTGCGGTCGCTTCTGGGATGGTTCCGGTTGCAACCGCTTCTGACGGTGGGGGATCGATCCGAATTCC
TGCGGGTTACTGTGGTTTGTTCGGTTTGAAACCTACTCGAGGGAGAACTCCAGTTGGTCCTAATTTTGGTAGATTTTGGC
AAGGTGCTTCTGTGGATCATATTCTTTCTAGATCTGTCAGAGACAGCGCGGCTTTTTTGGACGCGTTATGCGGAATTGAA
AAGTCGGGAGCTTTTTCTTTTGAGGAACCGAAGACGAACTATCTTTCCGAGATTAAAAAATCTACTGGCAAACTCAAAAT
CGCTTTTTCCACCCAATCTCCGATTGGGACTAAGGTTCATCCGGATTGTGTGGAATCTGTGATCCAAACCGCAAAACTAC
TACATTCTCTTGGTCATAAGGTAGAAGAAAAGGACGCACCTATCGACGGTAAGGCGATCGGGAGATCTTTTATCACGATG
TATTTCGGTGAGGTTGCCGCTCAGTTAAAAAGCTTAGAAACGATACTCGGGCGAAAAGTCAGAATGAATGACGTTGAACC
TGTCACTTGGATTTTAGGCCTTTTAGGTAGAACTATTTCTGGGGGTGAGTTTGTTCTTAATTTACAAGAATGGGATAAGG
CCGCCTTGGCAATGGAAACTTTTCACGAAACCTACGATGTTTATTTGACTCCTACGACTGCCATGCCTCCTTCTAAAATA
GGTGAATTAGAACCTAAGTTAGGCGAAAAGATAGCGATGCAAATAGTAGGTCATCTTGGTTTAGGAAAAATTCTATTCGC
TTCCGGGCTTGTGGACGAACTCGTGGAAAAAAGCCTTTCTAGAACTCCGTTTACTCAACTCGCTAATCTTACTGGTCAAC
CTTCTGTTTCCATTCCTCTAAGCCAGACGAGTGATCGGCTTCCCTTGGGGCTTTTATTTACTGCGGCTAGAAGAAGAGAA
GATATTTTATTTCGCCTCAGTTCTCAATTGGAAAAAGCGGTTCCTTGGTCGAATCATAAATGA

Upstream 100 bases:

>100_bases
TTTGCGACGTGTATATCCACATTTTGGGAACTTCTTTTTTACAGGAATCTTAAAAACTTCCTTTTTATCTTGACCCTCTT
TTCTTTTTGAGGTTCCTAAA

Downstream 100 bases:

>100_bases
ATGAGAAGAAAATGATTACGGGTCTCCGTTTTTAGAAAGAGAGTATTTCCTGTTTATTGGAAATATCATTTGGGATGATT
ACAAAAATAGAATGAATCTT

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MYSESPMPQPIPEYTYYDAIGLATLVRKKLIHPIELVESAIQRIETINPGLNAVITRFYEEARKTSKSKLPNGPFRGVPI
LVKDMVHSIGGAPLTCGSKAFRNYISPQDSEFIKRLRKTGTIFLGQTNVPEFALMGITEPKLYGPTRNPWNLERTPGGSS
GGSAAAVASGMVPVATASDGGGSIRIPAGYCGLFGLKPTRGRTPVGPNFGRFWQGASVDHILSRSVRDSAAFLDALCGIE
KSGAFSFEEPKTNYLSEIKKSTGKLKIAFSTQSPIGTKVHPDCVESVIQTAKLLHSLGHKVEEKDAPIDGKAIGRSFITM
YFGEVAAQLKSLETILGRKVRMNDVEPVTWILGLLGRTISGGEFVLNLQEWDKAALAMETFHETYDVYLTPTTAMPPSKI
GELEPKLGEKIAMQIVGHLGLGKILFASGLVDELVEKSLSRTPFTQLANLTGQPSVSIPLSQTSDRLPLGLLFTAARRRE
DILFRLSSQLEKAVPWSNHK

Sequences:

>Translated_500_residues
MYSESPMPQPIPEYTYYDAIGLATLVRKKLIHPIELVESAIQRIETINPGLNAVITRFYEEARKTSKSKLPNGPFRGVPI
LVKDMVHSIGGAPLTCGSKAFRNYISPQDSEFIKRLRKTGTIFLGQTNVPEFALMGITEPKLYGPTRNPWNLERTPGGSS
GGSAAAVASGMVPVATASDGGGSIRIPAGYCGLFGLKPTRGRTPVGPNFGRFWQGASVDHILSRSVRDSAAFLDALCGIE
KSGAFSFEEPKTNYLSEIKKSTGKLKIAFSTQSPIGTKVHPDCVESVIQTAKLLHSLGHKVEEKDAPIDGKAIGRSFITM
YFGEVAAQLKSLETILGRKVRMNDVEPVTWILGLLGRTISGGEFVLNLQEWDKAALAMETFHETYDVYLTPTTAMPPSKI
GELEPKLGEKIAMQIVGHLGLGKILFASGLVDELVEKSLSRTPFTQLANLTGQPSVSIPLSQTSDRLPLGLLFTAARRRE
DILFRLSSQLEKAVPWSNHK
>Mature_500_residues
MYSESPMPQPIPEYTYYDAIGLATLVRKKLIHPIELVESAIQRIETINPGLNAVITRFYEEARKTSKSKLPNGPFRGVPI
LVKDMVHSIGGAPLTCGSKAFRNYISPQDSEFIKRLRKTGTIFLGQTNVPEFALMGITEPKLYGPTRNPWNLERTPGGSS
GGSAAAVASGMVPVATASDGGGSIRIPAGYCGLFGLKPTRGRTPVGPNFGRFWQGASVDHILSRSVRDSAAFLDALCGIE
KSGAFSFEEPKTNYLSEIKKSTGKLKIAFSTQSPIGTKVHPDCVESVIQTAKLLHSLGHKVEEKDAPIDGKAIGRSFITM
YFGEVAAQLKSLETILGRKVRMNDVEPVTWILGLLGRTISGGEFVLNLQEWDKAALAMETFHETYDVYLTPTTAMPPSKI
GELEPKLGEKIAMQIVGHLGLGKILFASGLVDELVEKSLSRTPFTQLANLTGQPSVSIPLSQTSDRLPLGLLFTAARRRE
DILFRLSSQLEKAVPWSNHK

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=255, Percent_Identity=31.7647058823529, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI222831590, Length=539, Percent_Identity=25.417439703154, Blast_Score=114, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=196, Percent_Identity=37.7551020408163, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=494, Percent_Identity=25.3036437246964, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=31.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=508, Percent_Identity=23.2283464566929, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=131, Percent_Identity=37.4045801526718, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=31.7948717948718, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=195, Percent_Identity=31.7948717948718, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=157, Percent_Identity=33.7579617834395, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=283, Percent_Identity=29.6819787985866, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=130, Percent_Identity=43.8461538461538, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=140, Percent_Identity=37.1428571428571, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=28.6919831223629, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=24.3027888446215, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45550774, Length=244, Percent_Identity=33.1967213114754, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=32.2448979591837, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=32.2448979591837, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356731, Length=236, Percent_Identity=33.8983050847458, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=26.7068273092369, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=28.0737704918033, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24652985, Length=516, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922090, Length=516, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652981, Length=516, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652983, Length=516, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 54411; Mature: 54411

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSESPMPQPIPEYTYYDAIGLATLVRKKLIHPIELVESAIQRIETINPGLNAVITRFYE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EARKTSKSKLPNGPFRGVPILVKDMVHSIGGAPLTCGSKAFRNYISPQDSEFIKRLRKTG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCC
TIFLGQTNVPEFALMGITEPKLYGPTRNPWNLERTPGGSSGGSAAAVASGMVPVATASDG
CEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCC
GGSIRIPAGYCGLFGLKPTRGRTPVGPNFGRFWQGASVDHILSRSVRDSAAFLDALCGIE
CCEEEECCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KSGAFSFEEPKTNYLSEIKKSTGKLKIAFSTQSPIGTKVHPDCVESVIQTAKLLHSLGHK
CCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCH
VEEKDAPIDGKAIGRSFITMYFGEVAAQLKSLETILGRKVRMNDVEPVTWILGLLGRTIS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
GGEFVLNLQEWDKAALAMETFHETYDVYLTPTTAMPPSKIGELEPKLGEKIAMQIVGHLG
CCEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHCC
LGKILFASGLVDELVEKSLSRTPFTQLANLTGQPSVSIPLSQTSDRLPLGLLFTAARRRE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
DILFRLSSQLEKAVPWSNHK
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYSESPMPQPIPEYTYYDAIGLATLVRKKLIHPIELVESAIQRIETINPGLNAVITRFYE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EARKTSKSKLPNGPFRGVPILVKDMVHSIGGAPLTCGSKAFRNYISPQDSEFIKRLRKTG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCC
TIFLGQTNVPEFALMGITEPKLYGPTRNPWNLERTPGGSSGGSAAAVASGMVPVATASDG
CEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCC
GGSIRIPAGYCGLFGLKPTRGRTPVGPNFGRFWQGASVDHILSRSVRDSAAFLDALCGIE
CCEEEECCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KSGAFSFEEPKTNYLSEIKKSTGKLKIAFSTQSPIGTKVHPDCVESVIQTAKLLHSLGHK
CCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCH
VEEKDAPIDGKAIGRSFITMYFGEVAAQLKSLETILGRKVRMNDVEPVTWILGLLGRTIS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
GGEFVLNLQEWDKAALAMETFHETYDVYLTPTTAMPPSKIGELEPKLGEKIAMQIVGHLG
CCEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHCC
LGKILFASGLVDELVEKSLSRTPFTQLANLTGQPSVSIPLSQTSDRLPLGLLFTAARRRE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
DILFRLSSQLEKAVPWSNHK
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]