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The map label for this gene is tetA [H]

Identifier: 45658207

GI number: 45658207

Start: 2861270

End: 2862499

Strand: Reverse

Name: tetA [H]

Synonym: LIC12361

Alternate gene names: 45658207

Gene position: 2862499-2861270 (Counterclockwise)

Preceding gene: 45658209

Following gene: 45658206

Centisome position: 66.92

GC content: 40.33

Gene sequence:

>1230_bases
TTGGGTGGAGTTATGAATCATAGACGACCTGCGGCTTTGGGTTTTATTTTTGTAACCATTTTGATTGATGTAATCGGATT
TGGAATTATTATTCCAGTTTTACCAAAACTCATTCAGGAATTGACTCATGGAACTTTGAGCCAAGCCGCGTGGTATGGTG
GGCTTTTGATGTTTGCGTATTCATTTGTGCAATTTGTTTGTGCTCCGTTTGTAGGTGGATTGAGTGATCGATACGGAAGA
AGGCCGGTACTACTCGCTTCTCTTTTTGGTTTTACGTTAGATTATTTATTCTTAGCTTTTGCACCTTCCATTTTTTGGTT
GTTTGTTGGAAGGATTTTAGCGGGAATCATGGGTGCGAGTTTTACAACTGGTTATGCCTATATCGCCGATATTAGTCCTC
CCGAAAAAAGAGCACAGAATTTTGGAATTTTAGGAGCGGCGTTTGGATTTGGATTTATCATAGGGCCGGTGATCGGAGGT
GTTCTAGGTCAATACGGTTCCAGAGCTCCGTTTTTAGCCGCGGCTGCCCTTACTCTTATAAATTGGTTGTTCGGTTTTTT
TATTCTGCCAGAATCTTTAACTCCTGAAAATAGAAGAAAATTTGAATGGCAAAAGGCAAATCCAATTGGATCTCTTATCA
ATCTGAAACGTTATCCTATGATCATAGGATTAGTAGTTGCATTTTTTCTAATGAACGTAGCGGCTCACTCCGTACAGGGA
ACTTGGAATTATTATACAATGGAAAAATTTCAATGGAACGAGGCGATGGTGGGTTATTCTTTAGGAGTTGTAGGTCTTGT
TTATGCAATCACACAAGGTGGATTGATTAGGATTATTCTTCCTGCGCTCGGACAGAATAAAAGTATTTATCTGGGACTGG
CTTTAAGTGGATTGGGATATGCGTTATTCGCTCTTGCAACACAAAGTTGGATGATGTTTGTATTTTTAATTCCTTATTGT
TTGGGAGGAATTGCAATGCCACCTCTTCAAGGGATCATGTCTTCTCAGGTTCCGTCAAATGAACAAGGAGAATTACAAGG
AGCTCTTACTAGTTTGATGAGTGTGACCGCAATTCTAGGGCCAATTTTAATGACGGGTTTGTTCTCCTATTTTACCGCAA
AAGGAACTCCTGTTTATTCACCAGAGGCACCTTTGTGGATGGGAACTATTTTGACGGCAGCGAGCCTTTGGATTACGGTT
GGATCTCTTAGAAAACATCATTCGGAATGA

Upstream 100 bases:

>100_bases
AAAAATTTTAACAGATTTGTATTTCGTTTTCAGTTTGACGGTTTGACGAAAAGGCTTACTCAAGAAATAATTTTTTCGTT
GTCAAAAGACTTAGGATTCA

Downstream 100 bases:

>100_bases
CCTTAAGTCTTAAGATGGGCTCTAGGCGATTTTTCGAAACAAGTTCCAAATTACTCTTTCTATTGGGTTTGATTTGTGCA
AAAATTTTTTAGAGTTAAGA

Product: permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(C) [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGGVMNHRRPAALGFIFVTILIDVIGFGIIIPVLPKLIQELTHGTLSQAAWYGGLLMFAYSFVQFVCAPFVGGLSDRYGR
RPVLLASLFGFTLDYLFLAFAPSIFWLFVGRILAGIMGASFTTGYAYIADISPPEKRAQNFGILGAAFGFGFIIGPVIGG
VLGQYGSRAPFLAAAALTLINWLFGFFILPESLTPENRRKFEWQKANPIGSLINLKRYPMIIGLVVAFFLMNVAAHSVQG
TWNYYTMEKFQWNEAMVGYSLGVVGLVYAITQGGLIRIILPALGQNKSIYLGLALSGLGYALFALATQSWMMFVFLIPYC
LGGIAMPPLQGIMSSQVPSNEQGELQGALTSLMSVTAILGPILMTGLFSYFTAKGTPVYSPEAPLWMGTILTAASLWITV
GSLRKHHSE

Sequences:

>Translated_409_residues
MGGVMNHRRPAALGFIFVTILIDVIGFGIIIPVLPKLIQELTHGTLSQAAWYGGLLMFAYSFVQFVCAPFVGGLSDRYGR
RPVLLASLFGFTLDYLFLAFAPSIFWLFVGRILAGIMGASFTTGYAYIADISPPEKRAQNFGILGAAFGFGFIIGPVIGG
VLGQYGSRAPFLAAAALTLINWLFGFFILPESLTPENRRKFEWQKANPIGSLINLKRYPMIIGLVVAFFLMNVAAHSVQG
TWNYYTMEKFQWNEAMVGYSLGVVGLVYAITQGGLIRIILPALGQNKSIYLGLALSGLGYALFALATQSWMMFVFLIPYC
LGGIAMPPLQGIMSSQVPSNEQGELQGALTSLMSVTAILGPILMTGLFSYFTAKGTPVYSPEAPLWMGTILTAASLWITV
GSLRKHHSE
>Mature_408_residues
GGVMNHRRPAALGFIFVTILIDVIGFGIIIPVLPKLIQELTHGTLSQAAWYGGLLMFAYSFVQFVCAPFVGGLSDRYGRR
PVLLASLFGFTLDYLFLAFAPSIFWLFVGRILAGIMGASFTTGYAYIADISPPEKRAQNFGILGAAFGFGFIIGPVIGGV
LGQYGSRAPFLAAAALTLINWLFGFFILPESLTPENRRKFEWQKANPIGSLINLKRYPMIIGLVVAFFLMNVAAHSVQGT
WNYYTMEKFQWNEAMVGYSLGVVGLVYAITQGGLIRIILPALGQNKSIYLGLALSGLGYALFALATQSWMMFVFLIPYCL
GGIAMPPLQGIMSSQVPSNEQGELQGALTSLMSVTAILGPILMTGLFSYFTAKGTPVYSPEAPLWMGTILTAASLWITVG
SLRKHHSE

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=329, Percent_Identity=25.2279635258359, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI94721311, Length=355, Percent_Identity=25.9154929577465, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI225703104, Length=277, Percent_Identity=26.7148014440433, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI225703102, Length=277, Percent_Identity=26.7148014440433, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI166064062, Length=294, Percent_Identity=27.5510204081633, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI166064060, Length=294, Percent_Identity=27.5510204081633, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI217416366, Length=143, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787291, Length=227, Percent_Identity=25.5506607929515, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=377, Percent_Identity=24.1379310344828, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17551570, Length=345, Percent_Identity=24.0579710144928, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357569, Length=373, Percent_Identity=24.3967828418231, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI221513297, Length=360, Percent_Identity=26.3888888888889, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656463, Length=374, Percent_Identity=24.331550802139, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24656468, Length=374, Percent_Identity=24.331550802139, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161080926, Length=374, Percent_Identity=24.331550802139, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161080924, Length=374, Percent_Identity=24.331550802139, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17738085, Length=338, Percent_Identity=28.9940828402367, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24667557, Length=160, Percent_Identity=30, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652013, Length=325, Percent_Identity=26.4615384615385, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667561, Length=156, Percent_Identity=30.1282051282051, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24652011, Length=318, Percent_Identity=24.2138364779874, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471713, Length=157, Percent_Identity=34.3949044585987, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471717, Length=157, Percent_Identity=34.3949044585987, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471715, Length=157, Percent_Identity=34.3949044585987, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44413; Mature: 44282

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GGVMNHRRPAALGFIFVTILIDVIGFGIIIPVLPKLIQELTHGTLSQAAWYGGLLMFAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SFVQFVCAPFVGGLSDRYGRRPVLLASLFGFTLDYLFLAFAPSIFWLFVGRILAGIMGAS
HHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FTTGYAYIADISPPEKRAQNFGILGAAFGFGFIIGPVIGGVLGQYGSRAPFLAAAALTLI
CCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NWLFGFFILPESLTPENRRKFEWQKANPIGSLINLKRYPMIIGLVVAFFLMNVAAHSVQG
HHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TWNYYTMEKFQWNEAMVGYSLGVVGLVYAITQGGLIRIILPALGQNKSIYLGLALSGLGY
CCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCEEEEEHHHHHHH
ALFALATQSWMMFVFLIPYCLGGIAMPPLQGIMSSQVPSNEQGELQGALTSLMSVTAILG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
PILMTGLFSYFTAKGTPVYSPEAPLWMGTILTAASLWITVGSLRKHHSE
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 383387; 6337373; 6307828; 1517220 [H]