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The map label for this gene is kdtA

Identifier: 45658128

GI number: 45658128

Start: 2751342

End: 2752598

Strand: Direct

Name: kdtA

Synonym: LIC12280

Alternate gene names: 45658128

Gene position: 2751342-2752598 (Clockwise)

Preceding gene: 45658126

Following gene: 45658131

Centisome position: 64.33

GC content: 36.75

Gene sequence:

>1257_bases
ATGATTTTTCTTTATCAAATCCTGACGATCTTTCTTCTTATTTTTGTTGTTCCGATTTCTTTCTTATTTCCTTCCGCGAG
ACTTTTTTTTAGAAAACGTTCTGCAGATAAGAAAAAAATTCTTTCTAAGTCACTGGACCTTTCCGGTAAATATACTGTTT
GGCTTCACGCTGCTTCGGTTGGTGAATTGGACCAGTGTAAGGCACTTGCACTTGAATTTAGAAAAAACGATCCTTCTGCA
TTTTTAATTCAATCTGTTTTTTCGGATAGTGTAAGAGATTCTCAATTAGAAGCATTTCCAGCGGATGAAACTTTTCATCT
TCCGATCGATCTTCCTTTTAGTTATGATTGGATTTTTTCCCGTTTTCACCCTAAGGTTTTAGTTTTGATGGCTTGGGATA
CCTGGCCAAATTTAGTAATTTCTGCAAATAGATTTAACACCAAAGTAGTGTTAGGTTCTGCGGTAATCGGAAATCGTAAA
AAAGGGATTATGGGCAAATTGACTAAATCCGTTTTTAAACATCTGGATGGAATTTTTCCTTCTCACGAATCTTTTTACGA
TGTTTTTCGTTCTTTGGTCCCGGATCAAATTCCTGTAAAGGTGTTAGGTGATACTAGATTCGATTCCGTTCTCAAAAAAA
TCGAAGACAATGCTAAAATTTTTAAAAAACCTAAAAATTATCCGTATTCTAAAATTATACTTTTTGCATCTACTTACGAA
CCCTGCGAAAACTTAATCGTTTCTCTTTATGAACTCATCCGTTCAAAAAACCCTGCCTTATTAGACAATTTTGCATTTTG
GATTTTTCCCCATAAAACTTCTCCCGATAGGATTATTTCTATAGAACACAAACTACAAGATGCAAATATAATTTACCAAA
CCTGGACTTCTACTCCTTTTGAAAACCTGACGGCTCAGACAATCGTATTTGACGTGTTAGGCGTATTAGCATTTGCGTAT
CAAGCAGCGGATTTTGCCTATGTGGGAGGTGCACTTCACAATAGAGTGCACAACATTTTGGAACCAGCTACTTTTGGCCT
GCCTCTGATGACCGGCCCTAAAATTTATAATTCTCCAGAAGCTATGATTTTGGAAAAATCGGGCAGTCTATTTATCGTCT
CCGAAGCGGAAGACATATTCCAAATTTTGAATTTGTCTGAAAACGATCTGGAAACGATTCGAAAACAAAACTGGGAATTC
GTCCAATCGGGGCGAGGAGCGGCGAAAAGATTGTATGAAGAGATTCGGAAGTTATAA

Upstream 100 bases:

>100_bases
TCGATTCGTTTGGGAGCATTTTATAGAGTGCAGAATGGAAAGGATCTTTAATTAAAGTATCCATCAAATCAAATGCAAAT
AAAACTCCGCTCATAGAACT

Downstream 100 bases:

>100_bases
TTTTTTCTCAGCCAAAAGTTTTTGCTTAAGGCTTGTCCCGAAACCTCAAAAAATTTTACACTATAATTCTTTAGAAATTT
TTAATAAAATGCAGTAGTTC

Product: 3-deoxy-d-manno-octulosonic acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIFLYQILTIFLLIFVVPISFLFPSARLFFRKRSADKKKILSKSLDLSGKYTVWLHAASVGELDQCKALALEFRKNDPSA
FLIQSVFSDSVRDSQLEAFPADETFHLPIDLPFSYDWIFSRFHPKVLVLMAWDTWPNLVISANRFNTKVVLGSAVIGNRK
KGIMGKLTKSVFKHLDGIFPSHESFYDVFRSLVPDQIPVKVLGDTRFDSVLKKIEDNAKIFKKPKNYPYSKIILFASTYE
PCENLIVSLYELIRSKNPALLDNFAFWIFPHKTSPDRIISIEHKLQDANIIYQTWTSTPFENLTAQTIVFDVLGVLAFAY
QAADFAYVGGALHNRVHNILEPATFGLPLMTGPKIYNSPEAMILEKSGSLFIVSEAEDIFQILNLSENDLETIRKQNWEF
VQSGRGAAKRLYEEIRKL

Sequences:

>Translated_418_residues
MIFLYQILTIFLLIFVVPISFLFPSARLFFRKRSADKKKILSKSLDLSGKYTVWLHAASVGELDQCKALALEFRKNDPSA
FLIQSVFSDSVRDSQLEAFPADETFHLPIDLPFSYDWIFSRFHPKVLVLMAWDTWPNLVISANRFNTKVVLGSAVIGNRK
KGIMGKLTKSVFKHLDGIFPSHESFYDVFRSLVPDQIPVKVLGDTRFDSVLKKIEDNAKIFKKPKNYPYSKIILFASTYE
PCENLIVSLYELIRSKNPALLDNFAFWIFPHKTSPDRIISIEHKLQDANIIYQTWTSTPFENLTAQTIVFDVLGVLAFAY
QAADFAYVGGALHNRVHNILEPATFGLPLMTGPKIYNSPEAMILEKSGSLFIVSEAEDIFQILNLSENDLETIRKQNWEF
VQSGRGAAKRLYEEIRKL
>Mature_418_residues
MIFLYQILTIFLLIFVVPISFLFPSARLFFRKRSADKKKILSKSLDLSGKYTVWLHAASVGELDQCKALALEFRKNDPSA
FLIQSVFSDSVRDSQLEAFPADETFHLPIDLPFSYDWIFSRFHPKVLVLMAWDTWPNLVISANRFNTKVVLGSAVIGNRK
KGIMGKLTKSVFKHLDGIFPSHESFYDVFRSLVPDQIPVKVLGDTRFDSVLKKIEDNAKIFKKPKNYPYSKIILFASTYE
PCENLIVSLYELIRSKNPALLDNFAFWIFPHKTSPDRIISIEHKLQDANIIYQTWTSTPFENLTAQTIVFDVLGVLAFAY
QAADFAYVGGALHNRVHNILEPATFGLPLMTGPKIYNSPEAMILEKSGSLFIVSEAEDIFQILNLSENDLETIRKQNWEF
VQSGRGAAKRLYEEIRKL

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=394, Percent_Identity=24.3654822335025, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47769; Mature: 47769

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLYQILTIFLLIFVVPISFLFPSARLFFRKRSADKKKILSKSLDLSGKYTVWLHAASV
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCC
GELDQCKALALEFRKNDPSAFLIQSVFSDSVRDSQLEAFPADETFHLPIDLPFSYDWIFS
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHH
RFHPKVLVLMAWDTWPNLVISANRFNTKVVLGSAVIGNRKKGIMGKLTKSVFKHLDGIFP
HCCCEEEEEEEECCCCCEEEECCCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCC
SHESFYDVFRSLVPDQIPVKVLGDTRFDSVLKKIEDNAKIFKKPKNYPYSKIILFASTYE
CHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCC
PCENLIVSLYELIRSKNPALLDNFAFWIFPHKTSPDRIISIEHKLQDANIIYQTWTSTPF
HHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCEEEEHHHCCCCEEEEEECCCCCH
ENLTAQTIVFDVLGVLAFAYQAADFAYVGGALHNRVHNILEPATFGLPLMTGPKIYNSPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCC
AMILEKSGSLFIVSEAEDIFQILNLSENDLETIRKQNWEFVQSGRGAAKRLYEEIRKL
EEEEECCCCEEEEECHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIFLYQILTIFLLIFVVPISFLFPSARLFFRKRSADKKKILSKSLDLSGKYTVWLHAASV
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCC
GELDQCKALALEFRKNDPSAFLIQSVFSDSVRDSQLEAFPADETFHLPIDLPFSYDWIFS
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHH
RFHPKVLVLMAWDTWPNLVISANRFNTKVVLGSAVIGNRKKGIMGKLTKSVFKHLDGIFP
HCCCEEEEEEEECCCCCEEEECCCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCC
SHESFYDVFRSLVPDQIPVKVLGDTRFDSVLKKIEDNAKIFKKPKNYPYSKIILFASTYE
CHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCC
PCENLIVSLYELIRSKNPALLDNFAFWIFPHKTSPDRIISIEHKLQDANIIYQTWTSTPF
HHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCEEEEHHHCCCCEEEEEECCCCCH
ENLTAQTIVFDVLGVLAFAYQAADFAYVGGALHNRVHNILEPATFGLPLMTGPKIYNSPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCC
AMILEKSGSLFIVSEAEDIFQILNLSENDLETIRKQNWEFVQSGRGAAKRLYEEIRKL
EEEEECCCCEEEEECHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]