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The map label for this gene is nadB

Identifier: 45658100

GI number: 45658100

Start: 2714683

End: 2716281

Strand: Direct

Name: nadB

Synonym: LIC12251

Alternate gene names: 45658100

Gene position: 2714683-2716281 (Clockwise)

Preceding gene: 45658099

Following gene: 45658104

Centisome position: 63.47

GC content: 40.71

Gene sequence:

>1599_bases
ATGCCCCGTATCAAAACGGACTTTTTAGTCCTAGGGAGCGGAATTACAGGTCTTTTTGCGGCCTTAAAACTCGCTCCCTA
CGGCTCCGTAATCATAATCACAAAAAAATCGGACTATGAATCCAACACAAACTATGCACAAGGTGGGATCGCTTCCGTAT
TTGCAGAAGGAGATAAGCTAGAAGATCACGTAAGAGATACTCTAGAAGCAGGAGCCTGGCTTTGTGATCCTGCGGCGGTT
CAAGTTTTAGTAGAAGAAGGGCCTCCTCTAGTAAAAGAACTTTTAAATTATGGAGTTCCGTTCAATTTAGAACCTTCCGG
TAAATTTGATCTTTCCAGAGAAGGGGGGCACGGTAAAAACCGAATTGTACACGCACACGACAGAACCGGAAGAGAAATCG
AAAAAACTCTTTTAAATGTAGTAAAACAAAATATAAACATTCAGATATTAGAATATCATACTTTAGTAGATTTAATTACT
CCACATCATTTAAAACAAAAAGGTTTAATCTGTTACGGGGCCTACGTCCTTTCCAACCATACGGGAGAAGTATTTCCTAT
TTTAGCAAGAGAAACAATTCTTGCCACCGGCGGAGCAGGCCAGGTTTATTCTCATACCACTAATCCTAAAATCGCTACCG
GAGACGGTGTAGCTTCTGCATACAGAGCGGGTGCCCTGATTAAAAACATGGAATTCTATCAATTTCATCCTACTGCACTT
TATCACAAAGACGGAAATTCTTTTTTGATCTCAGAAGCGGTACGTGGTAAAGGAGCAATTTTACTCAATCGAGACGGCGA
AACGTTTATGAAACGTTATCATCCTATGGGCGATCTGGCTCCTAGGGACGTGGTAGCAAGAGCCATAGATTCAGAGATGA
AAAAAAGAGGAGAAGCTCATGTTTGGTTGGACGTTACACACCTTCCTTCTTCTCAAATTAGAGAATCCTTTCCTTCCATT
TATGAAAAATGTAAGGAACTGGGTATTGATATCACCACGGATCGGATTCCGGTCGTTCCGGTTGCACATTTTTTATGTGG
AGGTGTAGCTTCGGATTTAGAAGGTAGAACTACGATTTCTAATCTTTCAGCAGCTGGAGAAACCGCTTGTACTGGTGTTC
ACGGTGGCAACCGTCTTGCTTCTAACAGTCTTTTAGAATGTCTCGTATTCTCTAATCGGATCGCTGGAAGAATTTCAAAA
GAAAGTCCGAAATTTACCAAAGCACACGATGAAATTCCTTCTTGGAACAAAGAGGGTATGGTCAACACAGAAGAGTGGGT
TTTGATTTCTCACGATCTAAACGAAATTAAAAATACGATGAGCAATTACGTTGGAATCGTCCGCTCTAATCTCAGATTAG
AAAGGGCAAAACGTAGAATGGATCTGATCTACGACGAAGTAAAGGACTACTACAATCGAACCGTGATCACAAATCCACTC
ATCGAATTGCGTAACTTGGTAATTGTAGGAGAACTGATCATTCGTTCTGCACTTGCCAGAAAAGAAAGTAGAGGTCTTCA
TTTTTCCACAGATTATCCTGAAAATAGAAATCCTTCTAGAATAGATACGGAAATCAGAAACGAAAAAGTTCTTCTTTGA

Upstream 100 bases:

>100_bases
ATTGCGTCTCATAAAAACGATCCTATCAAGCCGGACTACCAATTGATCCGTTCCTTGGATTATATAGAAGCCTTACTCTC
TACACAAAAAAAGAAATAAA

Downstream 100 bases:

>100_bases
TTTAGAAATTGTGGGAACTATTACAAAATCTGAATCTACTGTTAAATTCTGAAATTGTGGGAACTATTACAAAATCTGAA
TCTACTATTAAATTCTGAAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MPRIKTDFLVLGSGITGLFAALKLAPYGSVIIITKKSDYESNTNYAQGGIASVFAEGDKLEDHVRDTLEAGAWLCDPAAV
QVLVEEGPPLVKELLNYGVPFNLEPSGKFDLSREGGHGKNRIVHAHDRTGREIEKTLLNVVKQNINIQILEYHTLVDLIT
PHHLKQKGLICYGAYVLSNHTGEVFPILARETILATGGAGQVYSHTTNPKIATGDGVASAYRAGALIKNMEFYQFHPTAL
YHKDGNSFLISEAVRGKGAILLNRDGETFMKRYHPMGDLAPRDVVARAIDSEMKKRGEAHVWLDVTHLPSSQIRESFPSI
YEKCKELGIDITTDRIPVVPVAHFLCGGVASDLEGRTTISNLSAAGETACTGVHGGNRLASNSLLECLVFSNRIAGRISK
ESPKFTKAHDEIPSWNKEGMVNTEEWVLISHDLNEIKNTMSNYVGIVRSNLRLERAKRRMDLIYDEVKDYYNRTVITNPL
IELRNLVIVGELIIRSALARKESRGLHFSTDYPENRNPSRIDTEIRNEKVLL

Sequences:

>Translated_532_residues
MPRIKTDFLVLGSGITGLFAALKLAPYGSVIIITKKSDYESNTNYAQGGIASVFAEGDKLEDHVRDTLEAGAWLCDPAAV
QVLVEEGPPLVKELLNYGVPFNLEPSGKFDLSREGGHGKNRIVHAHDRTGREIEKTLLNVVKQNINIQILEYHTLVDLIT
PHHLKQKGLICYGAYVLSNHTGEVFPILARETILATGGAGQVYSHTTNPKIATGDGVASAYRAGALIKNMEFYQFHPTAL
YHKDGNSFLISEAVRGKGAILLNRDGETFMKRYHPMGDLAPRDVVARAIDSEMKKRGEAHVWLDVTHLPSSQIRESFPSI
YEKCKELGIDITTDRIPVVPVAHFLCGGVASDLEGRTTISNLSAAGETACTGVHGGNRLASNSLLECLVFSNRIAGRISK
ESPKFTKAHDEIPSWNKEGMVNTEEWVLISHDLNEIKNTMSNYVGIVRSNLRLERAKRRMDLIYDEVKDYYNRTVITNPL
IELRNLVIVGELIIRSALARKESRGLHFSTDYPENRNPSRIDTEIRNEKVLL
>Mature_531_residues
PRIKTDFLVLGSGITGLFAALKLAPYGSVIIITKKSDYESNTNYAQGGIASVFAEGDKLEDHVRDTLEAGAWLCDPAAVQ
VLVEEGPPLVKELLNYGVPFNLEPSGKFDLSREGGHGKNRIVHAHDRTGREIEKTLLNVVKQNINIQILEYHTLVDLITP
HHLKQKGLICYGAYVLSNHTGEVFPILARETILATGGAGQVYSHTTNPKIATGDGVASAYRAGALIKNMEFYQFHPTALY
HKDGNSFLISEAVRGKGAILLNRDGETFMKRYHPMGDLAPRDVVARAIDSEMKKRGEAHVWLDVTHLPSSQIRESFPSIY
EKCKELGIDITTDRIPVVPVAHFLCGGVASDLEGRTTISNLSAAGETACTGVHGGNRLASNSLLECLVFSNRIAGRISKE
SPKFTKAHDEIPSWNKEGMVNTEEWVLISHDLNEIKNTMSNYVGIVRSNLRLERAKRRMDLIYDEVKDYYNRTVITNPLI
ELRNLVIVGELIIRSALARKESRGLHFSTDYPENRNPSRIDTEIRNEKVLL

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=519, Percent_Identity=33.718689788054, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1788928, Length=521, Percent_Identity=47.7927063339731, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1790597, Length=521, Percent_Identity=32.2456813819578, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=32.8571428571429, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17505833, Length=533, Percent_Identity=33.3958724202627, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17550100, Length=531, Percent_Identity=32.9566854990584, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71986328, Length=473, Percent_Identity=29.1754756871036, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=527, Percent_Identity=34.3453510436433, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6322416, Length=514, Percent_Identity=33.2684824902724, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.734693877551, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137288, Length=520, Percent_Identity=34.2307692307692, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24655642, Length=520, Percent_Identity=34.2307692307692, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24655647, Length=520, Percent_Identity=34.2307692307692, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24663005, Length=520, Percent_Identity=31.3461538461538, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59034; Mature: 58903

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIKTDFLVLGSGITGLFAALKLAPYGSVIIITKKSDYESNTNYAQGGIASVFAEGDKL
CCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCHH
EDHVRDTLEAGAWLCDPAAVQVLVEEGPPLVKELLNYGVPFNLEPSGKFDLSREGGHGKN
HHHHHHHHHCCCEEECHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
RIVHAHDRTGREIEKTLLNVVKQNINIQILEYHTLVDLITPHHLKQKGLICYGAYVLSNH
CEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHCCEEEEEEEEEECC
TGEVFPILARETILATGGAGQVYSHTTNPKIATGDGVASAYRAGALIKNMEFYQFHPTAL
CCCEEEEEECCEEEEECCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCEEEEECCEEE
YHKDGNSFLISEAVRGKGAILLNRDGETFMKRYHPMGDLAPRDVVARAIDSEMKKRGEAH
EEECCCEEEEEHHHCCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCE
VWLDVTHLPSSQIRESFPSIYEKCKELGIDITTDRIPVVPVAHFLCGGVASDLEGRTTIS
EEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCHHHCCCCCHHHH
NLSAAGETACTGVHGGNRLASNSLLECLVFSNRIAGRISKESPKFTKAHDEIPSWNKEGM
HHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCC
VNTEEWVLISHDLNEIKNTMSNYVGIVRSNLRLERAKRRMDLIYDEVKDYYNRTVITNPL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHH
IELRNLVIVGELIIRSALARKESRGLHFSTDYPENRNPSRIDTEIRNEKVLL
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PRIKTDFLVLGSGITGLFAALKLAPYGSVIIITKKSDYESNTNYAQGGIASVFAEGDKL
CCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCHH
EDHVRDTLEAGAWLCDPAAVQVLVEEGPPLVKELLNYGVPFNLEPSGKFDLSREGGHGKN
HHHHHHHHHCCCEEECHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
RIVHAHDRTGREIEKTLLNVVKQNINIQILEYHTLVDLITPHHLKQKGLICYGAYVLSNH
CEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHCCEEEEEEEEEECC
TGEVFPILARETILATGGAGQVYSHTTNPKIATGDGVASAYRAGALIKNMEFYQFHPTAL
CCCEEEEEECCEEEEECCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCEEEEECCEEE
YHKDGNSFLISEAVRGKGAILLNRDGETFMKRYHPMGDLAPRDVVARAIDSEMKKRGEAH
EEECCCEEEEEHHHCCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCE
VWLDVTHLPSSQIRESFPSIYEKCKELGIDITTDRIPVVPVAHFLCGGVASDLEGRTTIS
EEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCHHHCCCCCHHHH
NLSAAGETACTGVHGGNRLASNSLLECLVFSNRIAGRISKESPKFTKAHDEIPSWNKEGM
HHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCC
VNTEEWVLISHDLNEIKNTMSNYVGIVRSNLRLERAKRRMDLIYDEVKDYYNRTVITNPL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHH
IELRNLVIVGELIIRSALARKESRGLHFSTDYPENRNPSRIDTEIRNEKVLL
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]