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The map label for this gene is miaA

Identifier: 45658095

GI number: 45658095

Start: 2709428

End: 2710321

Strand: Reverse

Name: miaA

Synonym: LIC12246

Alternate gene names: 45658095

Gene position: 2710321-2709428 (Counterclockwise)

Preceding gene: 45658096

Following gene: 45658094

Centisome position: 63.37

GC content: 39.6

Gene sequence:

>894_bases
TTGACTTTTCCGATTTTGATCCTTGCCGCTCCGACCGGAGCGGGTAAAACTTCTCTCATTACCGAACTGGATCCTACACG
GTTCGAAATTCTTTCTTTTGATTCCAGACAAATTTATAGGGAAATGCCGATTGGAACCGCTGCCCCCACAAAAGAGCAGC
AGTCAAAAATTCGTCATCACCTCGTAGAAGTTTTATCTCCTAAGGAAAGTATAGACGCCGGGCTTTACAATCGAATGGCG
GAAGAAGCTTTGCAAAAAGTATTAAATACCGATAAAATTCCAGTTTTTACTGCGGGCACTGGTTTTTATCTAAAGGCGTT
TTTGTTTGGAATGTTTCCAGTGCCGGAGATCAGTGTTTCCGTTCGAGAACGGGTTCTTTCTATGAGTATAGAAGAAAAAA
AGGTTCTTTTGAATGACTTAGATCCGGAGGCTTTGGATAAAATATTTTCAGGAGACGATTATAGATTGGGTAGGGCTTTA
GAAGTCAATTTGATGGGAGAGAAATGGTCCCGTTTGAAAATTGATCCAAATACTTCTGCGATTTATAAATACAATTTGGA
AATTCGTCTGGGCATTTTTTTGGATCTAGATCGGAAAGAACTTTATGAAAGAATTAACCTAAGAGCTAAACGGATGATCG
ATTTGGGAATGGCAGATGAGGCTTGGGAGATTAGAGAAAGATACGGAGAATCTTGTCCTGGCCTCAAATCTTTAGGCTAT
AATTTTGCACTTGAAAATAAAAAAGGAAACTCCAATGTAGAGACATTCCTTGTGGATTTAAGTCAATCTCACAGGAATTA
TGCCAAAAGACAGATCACTTGGTTTCGAAAAGAAAACTACATCCAAGCTATGGATCGGTCCGAGGCGCTGGAACGGATCA
AACATATAAAATAA

Upstream 100 bases:

>100_bases
AATTGAAGACGATGAAATGGAGTTCACCGAAGAACCACTGGAAGAAGCCTTGGAGGAAGAAGATGACGATGCGGAAGAAC
AGGAAGAGTAATCTTCTTCT

Downstream 100 bases:

>100_bases
ACGGAAATAAAAAAAGATGTCTGCTAAAAACAATATACAGGACCAACTCTTAAACACTGCCCGGAAGGATAAATTGGATC
TTACGATTTATCTACTGAAC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTFPILILAAPTGAGKTSLITELDPTRFEILSFDSRQIYREMPIGTAAPTKEQQSKIRHHLVEVLSPKESIDAGLYNRMA
EEALQKVLNTDKIPVFTAGTGFYLKAFLFGMFPVPEISVSVRERVLSMSIEEKKVLLNDLDPEALDKIFSGDDYRLGRAL
EVNLMGEKWSRLKIDPNTSAIYKYNLEIRLGIFLDLDRKELYERINLRAKRMIDLGMADEAWEIRERYGESCPGLKSLGY
NFALENKKGNSNVETFLVDLSQSHRNYAKRQITWFRKENYIQAMDRSEALERIKHIK

Sequences:

>Translated_297_residues
MTFPILILAAPTGAGKTSLITELDPTRFEILSFDSRQIYREMPIGTAAPTKEQQSKIRHHLVEVLSPKESIDAGLYNRMA
EEALQKVLNTDKIPVFTAGTGFYLKAFLFGMFPVPEISVSVRERVLSMSIEEKKVLLNDLDPEALDKIFSGDDYRLGRAL
EVNLMGEKWSRLKIDPNTSAIYKYNLEIRLGIFLDLDRKELYERINLRAKRMIDLGMADEAWEIRERYGESCPGLKSLGY
NFALENKKGNSNVETFLVDLSQSHRNYAKRQITWFRKENYIQAMDRSEALERIKHIK
>Mature_296_residues
TFPILILAAPTGAGKTSLITELDPTRFEILSFDSRQIYREMPIGTAAPTKEQQSKIRHHLVEVLSPKESIDAGLYNRMAE
EALQKVLNTDKIPVFTAGTGFYLKAFLFGMFPVPEISVSVRERVLSMSIEEKKVLLNDLDPEALDKIFSGDDYRLGRALE
VNLMGEKWSRLKIDPNTSAIYKYNLEIRLGIFLDLDRKELYERINLRAKRMIDLGMADEAWEIRERYGESCPGLKSLGYN
FALENKKGNSNVETFLVDLSQSHRNYAKRQITWFRKENYIQAMDRSEALERIKHIK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=23.4726688102894, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790613, Length=288, Percent_Identity=28.8194444444444, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144712, Length=266, Percent_Identity=25.5639097744361, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=303, Percent_Identity=24.7524752475248, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24649830, Length=112, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LEPIC (Q72Q66)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002181.1
- ProteinModelPortal:   Q72Q66
- SMR:   Q72Q66
- GeneID:   2772866
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12246
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK573952
- BioCyc:   LINT267671:LIC_12246-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34190; Mature: 34059

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPILILAAPTGAGKTSLITELDPTRFEILSFDSRQIYREMPIGTAAPTKEQQSKIRHH
CCCEEEEEEECCCCCCCCEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHH
LVEVLSPKESIDAGLYNRMAEEALQKVLNTDKIPVFTAGTGFYLKAFLFGMFPVPEISVS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHH
VRERVLSMSIEEKKVLLNDLDPEALDKIFSGDDYRLGRALEVNLMGEKWSRLKIDPNTSA
HHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCEECCCEEEEEEECCCCCEEEECCCCCE
IYKYNLEIRLGIFLDLDRKELYERINLRAKRMIDLGMADEAWEIRERYGESCPGLKSLGY
EEEEEEEEEEEEEEECCHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCC
NFALENKKGNSNVETFLVDLSQSHRNYAKRQITWFRKENYIQAMDRSEALERIKHIK
CEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFPILILAAPTGAGKTSLITELDPTRFEILSFDSRQIYREMPIGTAAPTKEQQSKIRHH
CCEEEEEEECCCCCCCCEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHH
LVEVLSPKESIDAGLYNRMAEEALQKVLNTDKIPVFTAGTGFYLKAFLFGMFPVPEISVS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHH
VRERVLSMSIEEKKVLLNDLDPEALDKIFSGDDYRLGRALEVNLMGEKWSRLKIDPNTSA
HHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCEECCCEEEEEEECCCCCEEEECCCCCE
IYKYNLEIRLGIFLDLDRKELYERINLRAKRMIDLGMADEAWEIRERYGESCPGLKSLGY
EEEEEEEEEEEEEEECCHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCC
NFALENKKGNSNVETFLVDLSQSHRNYAKRQITWFRKENYIQAMDRSEALERIKHIK
CEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA