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The map label for this gene is wcaJ [H]

Identifier: 45657985

GI number: 45657985

Start: 2582919

End: 2584184

Strand: Reverse

Name: wcaJ [H]

Synonym: LIC12137

Alternate gene names: 45657985

Gene position: 2584184-2582919 (Counterclockwise)

Preceding gene: 45657986

Following gene: 45657984

Centisome position: 60.42

GC content: 32.31

Gene sequence:

>1266_bases
ATGTACGTTCGAAGACATTCTAGAATTCATGAACAAATACTTTTAAGTACTCTCTTTCAATTAACCGTTGGTAGTTTATT
AGTGTCTTGTGTTACTATAATTCCATTATGGGGATTCGATTTTTGGAATCGTATAGATAAAAATAGCTTCAATTCTGCGA
TGGGGGTTTTAATTGCTTTTAATATTACCTTTATTTCCTTAAGAAGATTATTAAAGTATCCTGGGTCTCAATCCTCTGCT
TATATCTTACCAACTACCGCAATTGTATTTGGGATTCTAGTTGCATTTAATTTAATAAATCGTGCTGAATATAGTATTCA
GGTAATTCTATTAGGTTTTATTACTACATTATCTTGGTGTTATATCGGATATTTTATAGGGCATCGATACCGTTTACAAA
GGTTTGCATTAGTTCCATTGGGTGAAGCTTTGGAGTTTAGGGAAACTCATGGGACACAGTTCGTCTTTTTGAAAAAACCT
GATTTGGGAAAAGAAAGATTTGATGCGGTAGTAGCGGATCTACGTGCAAAGAATTTGACTCCTGAATGGGAAAAGTTTTT
AGCAAGATGTACTTTATCGAGAATACCTGTTTATCATACTAAACAAATTATAGAATCATTGACTGGGAGGGTGAAAATTA
CTTACCTTTCTGAAAACGAATTCGGCTCTTTGCTGCCTTCTAAGTTTTATGAAACAATAAAACGTTTTATAGATTTTATA
GCTGCCTTATTCTTTTTTCCAATTTTTTCTCCTTTTATGTTTTTGATTGCAATTCTCATTCGTTTAGAAAGTAAAGGGAA
GGTTGTATTTTCTCAGAAAAGAATGGGGTACCGTGGAAGAATATTTACTCTTTATAAATTTAGAACGATGTATGTTGAAA
AAAAAGGAAAAGGATTTACTCAAGGAGAAAATGATTTAAGAATTACTAAAATTGGAAAAGTATTACGAAAATATCGACTT
GATGAAACATTGCAAATTTTTAATGTTTTAAAAGGAGATATGAGTTTTATCGGACCTAGACCTGAATCCATGGAACTTTC
AGAATGGTATGAAAAGGATGTTCCTTTTTTTGCTTATCGACATGTTGTTAGACCTGGTATATCCGGCTGGGCGCAAGTAG
AACAGGGGTACGCCGCCGAGGTAGATGGAATGAAAGTAAAGTTAGAATATGATTTTTATTATATAAAAAATTTTTCATTT
TGGTTAGATATGTTAATTACTTTTAAAACCGTTAAAACGATTTTGACAGGTTTTGGAGCACGATAA

Upstream 100 bases:

>100_bases
GACTAGAAAACAAATGGGTAGGAATGCAAATTTACTTCTTAAAAGTAAATTCTCTGTTGATAATGCTGCGGAGACAGTTT
TAAACATTCTTTCTTAATTT

Downstream 100 bases:

>100_bases
AAAAGTTTTAACGCATGCTAAATTCATTCAATCTTAGAAAAGTATTCTATTGTATGAAATTGAGAAAAAATTTATGAGCA
CTCACTTCTCATTAAAAAGT

Product: UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MYVRRHSRIHEQILLSTLFQLTVGSLLVSCVTIIPLWGFDFWNRIDKNSFNSAMGVLIAFNITFISLRRLLKYPGSQSSA
YILPTTAIVFGILVAFNLINRAEYSIQVILLGFITTLSWCYIGYFIGHRYRLQRFALVPLGEALEFRETHGTQFVFLKKP
DLGKERFDAVVADLRAKNLTPEWEKFLARCTLSRIPVYHTKQIIESLTGRVKITYLSENEFGSLLPSKFYETIKRFIDFI
AALFFFPIFSPFMFLIAILIRLESKGKVVFSQKRMGYRGRIFTLYKFRTMYVEKKGKGFTQGENDLRITKIGKVLRKYRL
DETLQIFNVLKGDMSFIGPRPESMELSEWYEKDVPFFAYRHVVRPGISGWAQVEQGYAAEVDGMKVKLEYDFYYIKNFSF
WLDMLITFKTVKTILTGFGAR

Sequences:

>Translated_421_residues
MYVRRHSRIHEQILLSTLFQLTVGSLLVSCVTIIPLWGFDFWNRIDKNSFNSAMGVLIAFNITFISLRRLLKYPGSQSSA
YILPTTAIVFGILVAFNLINRAEYSIQVILLGFITTLSWCYIGYFIGHRYRLQRFALVPLGEALEFRETHGTQFVFLKKP
DLGKERFDAVVADLRAKNLTPEWEKFLARCTLSRIPVYHTKQIIESLTGRVKITYLSENEFGSLLPSKFYETIKRFIDFI
AALFFFPIFSPFMFLIAILIRLESKGKVVFSQKRMGYRGRIFTLYKFRTMYVEKKGKGFTQGENDLRITKIGKVLRKYRL
DETLQIFNVLKGDMSFIGPRPESMELSEWYEKDVPFFAYRHVVRPGISGWAQVEQGYAAEVDGMKVKLEYDFYYIKNFSF
WLDMLITFKTVKTILTGFGAR
>Mature_421_residues
MYVRRHSRIHEQILLSTLFQLTVGSLLVSCVTIIPLWGFDFWNRIDKNSFNSAMGVLIAFNITFISLRRLLKYPGSQSSA
YILPTTAIVFGILVAFNLINRAEYSIQVILLGFITTLSWCYIGYFIGHRYRLQRFALVPLGEALEFRETHGTQFVFLKKP
DLGKERFDAVVADLRAKNLTPEWEKFLARCTLSRIPVYHTKQIIESLTGRVKITYLSENEFGSLLPSKFYETIKRFIDFI
AALFFFPIFSPFMFLIAILIRLESKGKVVFSQKRMGYRGRIFTLYKFRTMYVEKKGKGFTQGENDLRITKIGKVLRKYRL
DETLQIFNVLKGDMSFIGPRPESMELSEWYEKDVPFFAYRHVVRPGISGWAQVEQGYAAEVDGMKVKLEYDFYYIKNFSF
WLDMLITFKTVKTILTGFGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=183, Percent_Identity=40.4371584699454, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 49068; Mature: 49068

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVRRHSRIHEQILLSTLFQLTVGSLLVSCVTIIPLWGFDFWNRIDKNSFNSAMGVLIAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
NITFISLRRLLKYPGSQSSAYILPTTAIVFGILVAFNLINRAEYSIQVILLGFITTLSWC
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YIGYFIGHRYRLQRFALVPLGEALEFRETHGTQFVFLKKPDLGKERFDAVVADLRAKNLT
HHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
PEWEKFLARCTLSRIPVYHTKQIIESLTGRVKITYLSENEFGSLLPSKFYETIKRFIDFI
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
AALFFFPIFSPFMFLIAILIRLESKGKVVFSQKRMGYRGRIFTLYKFRTMYVEKKGKGFT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCEEEEEEHHHHHEEECCCCCC
QGENDLRITKIGKVLRKYRLDETLQIFNVLKGDMSFIGPRPESMELSEWYEKDVPFFAYR
CCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHCCCCHHHHH
HVVRPGISGWAQVEQGYAAEVDGMKVKLEYDFYYIKNFSFWLDMLITFKTVKTILTGFGA
HHHCCCCCHHHHHHCCCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure
MYVRRHSRIHEQILLSTLFQLTVGSLLVSCVTIIPLWGFDFWNRIDKNSFNSAMGVLIAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
NITFISLRRLLKYPGSQSSAYILPTTAIVFGILVAFNLINRAEYSIQVILLGFITTLSWC
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YIGYFIGHRYRLQRFALVPLGEALEFRETHGTQFVFLKKPDLGKERFDAVVADLRAKNLT
HHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
PEWEKFLARCTLSRIPVYHTKQIIESLTGRVKITYLSENEFGSLLPSKFYETIKRFIDFI
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
AALFFFPIFSPFMFLIAILIRLESKGKVVFSQKRMGYRGRIFTLYKFRTMYVEKKGKGFT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCEEEEEEHHHHHEEECCCCCC
QGENDLRITKIGKVLRKYRLDETLQIFNVLKGDMSFIGPRPESMELSEWYEKDVPFFAYR
CCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHCCCCHHHHH
HVVRPGISGWAQVEQGYAAEVDGMKVKLEYDFYYIKNFSFWLDMLITFKTVKTILTGFGA
HHHCCCCCHHHHHHCCCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]