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The map label for this gene is aphA [H]

Identifier: 45657935

GI number: 45657935

Start: 2525511

End: 2526965

Strand: Direct

Name: aphA [H]

Synonym: LIC12087

Alternate gene names: 45657935

Gene position: 2525511-2526965 (Clockwise)

Preceding gene: 45657934

Following gene: 45657936

Centisome position: 59.05

GC content: 36.15

Gene sequence:

>1455_bases
GTGACGATAAAACATTCAGAACTTACATTTCAATTGATGGTAGAATCTGTTCCAAATGCAATTCTATTATTAAATCGGGA
CGGTAAGATTGTTTATATCAACAATCAAGCCGAGAAATTATTCAAATACGAACGCACTGAGTTGATAGGACAAGTTGTAG
AAGAGCTTCTTCCTCATCGTTATCGCAAGAATCACCCGACTTTCAGAAATTCTTTTTTTCTTTCTCCTAAGGTTCGACCT
ATGGGTGCGGGCAGAGATCTTTTCGGTCTTAAAAAAGACGGAACAGAATTCCCAATAGAAATTGGTTTAAATCCAGTGGT
AACCGCAGATGGTACTCTCGTTTTGGCTTCTATTATAGACATTACAGAAAGAAAAAAAGCGGAACAGAGATTTCGTCTCG
TAGTAGAATCCGCGCCCAATGCGATGGTTTTAGTCAACTCGGAAGGAACAATTGCTCTCGTAAATACACAAACAGAAAAA
CTATTCGGTTACGAAAGAGAAGAATTGATTGGTGCAAAATTAGAAATATTACTACCAGAACGTTTTCGAGAAGCACATCC
AGATCGTAGAAATCAATTTTTTCTTTCTCCTACCGTTCGCTCTATGGGTGCAGGAAGAGATTTATTCGCCCGAAAAAAAA
ACGGCTCCGAAGTTCAGGTAGAAATCGGTCTCAACCCGATAGACACAGACGAAGGTATTATGGTTCTTGCTTCGATCATT
GACATTACGGAAAGAAAAAATCAAGAAACCACTTTGATTCGAAAAAACGAATTGGAAGTCAAAAACAAGGAACTGGAACA
ATTTGCATTTTTAGCGTCTCACGATTTACAAGAACCTTTAAGAACGGTATCTAACTATATTCAAGTTTTGGAAGAAGATT
ACGGCAACACATTTGATTCCAACGCAACCCGTTATCTAAAAACGATCGATCAAGCAACCAAACGGATGAGCGTTTTAGTA
AAAGCTCTTCTTATGTATGCAAGAATTGGTAGAGATCAAAAATTAGTGTTTACAGATTTAAATCGTATTCTTTCCGAAGT
TCTTTCGGATTTAGAAAATCTGATTCTCAATTCCAATGCAAAAATTATCTCAGATCCACTTCCAAGTATGAATGTATACG
AAGTAGAATTCAGACAACTCATTCAAAATCTAATTTCTAATGCGATCAAGTTTTCTAAAAAAGGAATCGATCCTGAAATT
AGAATTCGTTGTGAAAAAGAAGAAGACTACTGTCACTTTTCAGTTCAAGACAACGGAATCGGAATCGATTCCAAATATGT
AGATAGGATTTTCTTTATATTCCAAATACTGCATCTTAAAGAAGAATACGAAGGCCATGGGATCGGGTTAGCACATTGTA
AAAAAATTATAGAATTACACAATGGTCGTATTTGGGTCGAATCCGACTCGGAAGTAGGAAGTAATTTTCACTTTATCATT
CCTAATTTAAATTAA

Upstream 100 bases:

>100_bases
ATAAATTCAAATATTCTAATTTACTATTTGACACAATTTTACCATTGTGATACTTTTAGCGGGTTCAATTTCCCTTTTCC
TACTTTAAGAGTGAAATCCC

Downstream 100 bases:

>100_bases
ACTTATGAAAAACAAAAAGTTAAAATGTATTCTTCTCATTGACGATAACCAAGATGATAATTTTTTCCATGAAAGAGTTA
TCTATAAAGGAAGTTATGCG

Product: histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTIKHSELTFQLMVESVPNAILLLNRDGKIVYINNQAEKLFKYERTELIGQVVEELLPHRYRKNHPTFRNSFFLSPKVRP
MGAGRDLFGLKKDGTEFPIEIGLNPVVTADGTLVLASIIDITERKKAEQRFRLVVESAPNAMVLVNSEGTIALVNTQTEK
LFGYEREELIGAKLEILLPERFREAHPDRRNQFFLSPTVRSMGAGRDLFARKKNGSEVQVEIGLNPIDTDEGIMVLASII
DITERKNQETTLIRKNELEVKNKELEQFAFLASHDLQEPLRTVSNYIQVLEEDYGNTFDSNATRYLKTIDQATKRMSVLV
KALLMYARIGRDQKLVFTDLNRILSEVLSDLENLILNSNAKIISDPLPSMNVYEVEFRQLIQNLISNAIKFSKKGIDPEI
RIRCEKEEDYCHFSVQDNGIGIDSKYVDRIFFIFQILHLKEEYEGHGIGLAHCKKIIELHNGRIWVESDSEVGSNFHFII
PNLN

Sequences:

>Translated_484_residues
MTIKHSELTFQLMVESVPNAILLLNRDGKIVYINNQAEKLFKYERTELIGQVVEELLPHRYRKNHPTFRNSFFLSPKVRP
MGAGRDLFGLKKDGTEFPIEIGLNPVVTADGTLVLASIIDITERKKAEQRFRLVVESAPNAMVLVNSEGTIALVNTQTEK
LFGYEREELIGAKLEILLPERFREAHPDRRNQFFLSPTVRSMGAGRDLFARKKNGSEVQVEIGLNPIDTDEGIMVLASII
DITERKNQETTLIRKNELEVKNKELEQFAFLASHDLQEPLRTVSNYIQVLEEDYGNTFDSNATRYLKTIDQATKRMSVLV
KALLMYARIGRDQKLVFTDLNRILSEVLSDLENLILNSNAKIISDPLPSMNVYEVEFRQLIQNLISNAIKFSKKGIDPEI
RIRCEKEEDYCHFSVQDNGIGIDSKYVDRIFFIFQILHLKEEYEGHGIGLAHCKKIIELHNGRIWVESDSEVGSNFHFII
PNLN
>Mature_483_residues
TIKHSELTFQLMVESVPNAILLLNRDGKIVYINNQAEKLFKYERTELIGQVVEELLPHRYRKNHPTFRNSFFLSPKVRPM
GAGRDLFGLKKDGTEFPIEIGLNPVVTADGTLVLASIIDITERKKAEQRFRLVVESAPNAMVLVNSEGTIALVNTQTEKL
FGYEREELIGAKLEILLPERFREAHPDRRNQFFLSPTVRSMGAGRDLFARKKNGSEVQVEIGLNPIDTDEGIMVLASIID
ITERKNQETTLIRKNELEVKNKELEQFAFLASHDLQEPLRTVSNYIQVLEEDYGNTFDSNATRYLKTIDQATKRMSVLVK
ALLMYARIGRDQKLVFTDLNRILSEVLSDLENLILNSNAKIISDPLPSMNVYEVEFRQLIQNLISNAIKFSKKGIDPEIR
IRCEKEEDYCHFSVQDNGIGIDSKYVDRIFFIFQILHLKEEYEGHGIGLAHCKKIIELHNGRIWVESDSEVGSNFHFIIP
NLN

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG4251

COG function: function code T; Bacteriophytochrome (light-regulated signal transduction histidine kinase)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=360, Percent_Identity=25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=29.4820717131474, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786600, Length=359, Percent_Identity=26.1838440111421, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=25.5813953488372, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=29.2887029288703, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081921, Length=209, Percent_Identity=25.8373205741627, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=28.125, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55468; Mature: 55336

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKHSELTFQLMVESVPNAILLLNRDGKIVYINNQAEKLFKYERTELIGQVVEELLPHR
CCCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YRKNHPTFRNSFFLSPKVRPMGAGRDLFGLKKDGTEFPIEIGLNPVVTADGTLVLASIID
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHH
ITERKKAEQRFRLVVESAPNAMVLVNSEGTIALVNTQTEKLFGYEREELIGAKLEILLPE
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHCCCCEEEECCH
RFREAHPDRRNQFFLSPTVRSMGAGRDLFARKKNGSEVQVEIGLNPIDTDEGIMVLASII
HHHHCCCCCCCEEEECCHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
DITERKNQETTLIRKNELEVKNKELEQFAFLASHDLQEPLRTVSNYIQVLEEDYGNTFDS
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NATRYLKTIDQATKRMSVLVKALLMYARIGRDQKLVFTDLNRILSEVLSDLENLILNSNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
KIISDPLPSMNVYEVEFRQLIQNLISNAIKFSKKGIDPEIRIRCEKEEDYCHFSVQDNGI
CEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCC
GIDSKYVDRIFFIFQILHLKEEYEGHGIGLAHCKKIIELHNGRIWVESDSEVGSNFHFII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEE
PNLN
CCCC
>Mature Secondary Structure 
TIKHSELTFQLMVESVPNAILLLNRDGKIVYINNQAEKLFKYERTELIGQVVEELLPHR
CCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YRKNHPTFRNSFFLSPKVRPMGAGRDLFGLKKDGTEFPIEIGLNPVVTADGTLVLASIID
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHH
ITERKKAEQRFRLVVESAPNAMVLVNSEGTIALVNTQTEKLFGYEREELIGAKLEILLPE
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHCCCCEEEECCH
RFREAHPDRRNQFFLSPTVRSMGAGRDLFARKKNGSEVQVEIGLNPIDTDEGIMVLASII
HHHHCCCCCCCEEEECCHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
DITERKNQETTLIRKNELEVKNKELEQFAFLASHDLQEPLRTVSNYIQVLEEDYGNTFDS
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NATRYLKTIDQATKRMSVLVKALLMYARIGRDQKLVFTDLNRILSEVLSDLENLILNSNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
KIISDPLPSMNVYEVEFRQLIQNLISNAIKFSKKGIDPEIRIRCEKEEDYCHFSVQDNGI
CEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCC
GIDSKYVDRIFFIFQILHLKEEYEGHGIGLAHCKKIIELHNGRIWVESDSEVGSNFHFII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEE
PNLN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]