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The map label for this gene is topA

Identifier: 45657928

GI number: 45657928

Start: 2517693

End: 2519534

Strand: Reverse

Name: topA

Synonym: LIC12080

Alternate gene names: 45657928

Gene position: 2519534-2517693 (Counterclockwise)

Preceding gene: 45657929

Following gene: 45657925

Centisome position: 58.91

GC content: 34.36

Gene sequence:

>1842_bases
GTGTCTTTACTTATCATTGTAGAATCCCCATCCAAGGCAAAAACTATTGCTGGGTATTTAGGGAAAGAATTTAGAATTTT
AGCAACCTTAGGTCACGTAGCCGATCTACCTAAAACTACTCTTGGATTAGATTTAAAAAATCGTTTTGAACCGGAATACG
TAATTCTTCCCGGAAAAAAAAAGATACTTTCTGAAATCATAAAAACGGCAAAACAATCTCAAAAAGTTTTTCTGGCAACG
GACCCAGATCGGGAAGGGGAATTTATAAGCGCCTACATAAGAGATCGTTTACAAAAAAAATCAAACGTCTTTCGGATTCG
TTTTACAGAGGTTACCAAACAAGCGATTCTAAATTCGTTACAAAATCCAGATACGATTAATGAATTTTTGGTAGATGCAC
AAAAAACTAGAAGAATCGGAGATAGATTGATCGGTTATTTTATCAGTCCAGTGCTTTGGAAACAGATCGGTCCTGGATTA
TCCGCCGGAAGGGTACAATCTGTCGCGTTAAAATGGATCTGTGAAAGAGAAGAAGAAATTAGAAATTTTAAAATAGAAAT
ATATTATAATATACTATTGCATGGAACTGATCAAAAAGGAATAGTGGGAATTTTTAGTAGAACCGGCGATCGGATTTTTT
CCAAAGAAAAAGCTGATCAAATCCTACAAAATGTTCAAAAAGAAAAGGAACTTCGTATCTCCGAAAAAAAAGAAACTCTA
GGAAAACTTTTTCCTCCGCCTCCGTTTCAAACTGCTAGTTTACAACAAGAAGCTTTTAAGAAATTACGATTTTCTTCTAA
AAAAACAATGAGCCTCTCTCAGAGGCTTTATGAAGGAATGGATATAGGAAATGGCCAAAGAAATGGATTGATCACTTATA
TGAGAACGGATTCAGTACGTTTAAGTTCTGAGTTTGTTGAACGTGCAAGGTCTTGGATTTTATCCGAATTGGGTGAAACT
TTTGCCAGTCCACTTGAACGTAAAATTAGAAAATCAGCTAAAAAAATACAAGATGCTCATGAAGCAATTCGTGTTACGGA
TCCTTTTTTAACTCCAAAAGAAATAAAAAAGTTTTTAGGAAAAGAAGAGGCGGCTCTTTACGATTTGATTTGGAAACGAA
CTATTTCGTCCTTACTTCCTGCAGAAGAATTTATAAAAATAGAATATTCCATTTTTGCTGCCGGGGAATGTTTTCAATTA
GAAACCAAAAAAACTATTTTTCCAGGTTACAAAATATTAAACGAAGCGGATGTAAAAACTAATCCGAGTTGGGAAAAGGG
AGAATTATTTATCCTTCAGAAAGTGGAGTGTGAAAAAAAACAAACGGAACCGCCGTCTAGATATTCAGAAGGTACTTTAG
TCGCAAAATTAGAAAAACAAGGAATTGGTCGACCTTCCACTTATGCTACGGTTTCAGAAACACTTTTGAAACGAAAATAT
GTTTATGAAGAAAAGAAATTTTTTTATCCCTTCTCATTGGGAGAGAAGGTCAATTTCTTTTTACAATCTAGTTTTGGAGA
ACTCTTTCGAGAAAAGTTTACCGCCGAATTAGAATCAGATTTGGATCGAATTGAAAAAAAAGAGATAGATTCTAATTCTA
TATTAAACCGACTTTGGTTGGACCTACAAACTCAAATTCAAAATAGTAAATTCATTTTGTTTCAAAAAGAATGGGCTACG
GTTTTGCAAAAGAAAAAAGAGACTGGTTGGGGAATTTGTCCTGTTTGTAGAAATGGCATCCTTCAAAAGAAAAAAAGCTC
TCGAAAGAAAGAATTTTATCAATGCAATCGTTTCCCAGACTGTGAATTTGTAAGTTACGAACTGCCTGAATCTTTGGAAT
AA

Upstream 100 bases:

>100_bases
TAAAAACAATTTATTATTCGGTTCTAAAATCAAAAAACAGAATTTTAAAAAAAAGATATTTAGTTGAAAACCTGATAACA
AACCAAAATTTAAGAATTCG

Downstream 100 bases:

>100_bases
AACCACTCATAGTTATATAATAAAGCACCCGGTATCTTGCGTTAAAACGGAACGGGAACGGGTTCTTACAATAAAAACTT
ATGCTATTTAATTTTGTAGG

Product: DNA topoisomerase I protein

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSLLIIVESPSKAKTIAGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVILPGKKKILSEIIKTAKQSQKVFLAT
DPDREGEFISAYIRDRLQKKSNVFRIRFTEVTKQAILNSLQNPDTINEFLVDAQKTRRIGDRLIGYFISPVLWKQIGPGL
SAGRVQSVALKWICEREEEIRNFKIEIYYNILLHGTDQKGIVGIFSRTGDRIFSKEKADQILQNVQKEKELRISEKKETL
GKLFPPPPFQTASLQQEAFKKLRFSSKKTMSLSQRLYEGMDIGNGQRNGLITYMRTDSVRLSSEFVERARSWILSELGET
FASPLERKIRKSAKKIQDAHEAIRVTDPFLTPKEIKKFLGKEEAALYDLIWKRTISSLLPAEEFIKIEYSIFAAGECFQL
ETKKTIFPGYKILNEADVKTNPSWEKGELFILQKVECEKKQTEPPSRYSEGTLVAKLEKQGIGRPSTYATVSETLLKRKY
VYEEKKFFYPFSLGEKVNFFLQSSFGELFREKFTAELESDLDRIEKKEIDSNSILNRLWLDLQTQIQNSKFILFQKEWAT
VLQKKKETGWGICPVCRNGILQKKKSSRKKEFYQCNRFPDCEFVSYELPESLE

Sequences:

>Translated_613_residues
MSLLIIVESPSKAKTIAGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVILPGKKKILSEIIKTAKQSQKVFLAT
DPDREGEFISAYIRDRLQKKSNVFRIRFTEVTKQAILNSLQNPDTINEFLVDAQKTRRIGDRLIGYFISPVLWKQIGPGL
SAGRVQSVALKWICEREEEIRNFKIEIYYNILLHGTDQKGIVGIFSRTGDRIFSKEKADQILQNVQKEKELRISEKKETL
GKLFPPPPFQTASLQQEAFKKLRFSSKKTMSLSQRLYEGMDIGNGQRNGLITYMRTDSVRLSSEFVERARSWILSELGET
FASPLERKIRKSAKKIQDAHEAIRVTDPFLTPKEIKKFLGKEEAALYDLIWKRTISSLLPAEEFIKIEYSIFAAGECFQL
ETKKTIFPGYKILNEADVKTNPSWEKGELFILQKVECEKKQTEPPSRYSEGTLVAKLEKQGIGRPSTYATVSETLLKRKY
VYEEKKFFYPFSLGEKVNFFLQSSFGELFREKFTAELESDLDRIEKKEIDSNSILNRLWLDLQTQIQNSKFILFQKEWAT
VLQKKKETGWGICPVCRNGILQKKKSSRKKEFYQCNRFPDCEFVSYELPESLE
>Mature_612_residues
SLLIIVESPSKAKTIAGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVILPGKKKILSEIIKTAKQSQKVFLATD
PDREGEFISAYIRDRLQKKSNVFRIRFTEVTKQAILNSLQNPDTINEFLVDAQKTRRIGDRLIGYFISPVLWKQIGPGLS
AGRVQSVALKWICEREEEIRNFKIEIYYNILLHGTDQKGIVGIFSRTGDRIFSKEKADQILQNVQKEKELRISEKKETLG
KLFPPPPFQTASLQQEAFKKLRFSSKKTMSLSQRLYEGMDIGNGQRNGLITYMRTDSVRLSSEFVERARSWILSELGETF
ASPLERKIRKSAKKIQDAHEAIRVTDPFLTPKEIKKFLGKEEAALYDLIWKRTISSLLPAEEFIKIEYSIFAAGECFQLE
TKKTIFPGYKILNEADVKTNPSWEKGELFILQKVECEKKQTEPPSRYSEGTLVAKLEKQGIGRPSTYATVSETLLKRKYV
YEEKKFFYPFSLGEKVNFFLQSSFGELFREKFTAELESDLDRIEKKEIDSNSILNRLWLDLQTQIQNSKFILFQKEWATV
LQKKKETGWGICPVCRNGILQKKKSSRKKEFYQCNRFPDCEFVSYELPESLE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=595, Percent_Identity=24.7058823529412, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI4507635, Length=488, Percent_Identity=23.155737704918, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1787529, Length=590, Percent_Identity=34.4067796610169, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788061, Length=636, Percent_Identity=24.5283018867925, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=456, Percent_Identity=22.8070175438596, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32563869, Length=548, Percent_Identity=22.8102189781022, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585251, Length=581, Percent_Identity=23.2358003442341, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 70858; Mature: 70726

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLIIVESPSKAKTIAGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVILPGKK
CEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCEEEECCHH
KILSEIIKTAKQSQKVFLATDPDREGEFISAYIRDRLQKKSNVFRIRFTEVTKQAILNSL
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
QNPDTINEFLVDAQKTRRIGDRLIGYFISPVLWKQIGPGLSAGRVQSVALKWICEREEEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RNFKIEIYYNILLHGTDQKGIVGIFSRTGDRIFSKEKADQILQNVQKEKELRISEKKETL
CCEEEEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLFPPPPFQTASLQQEAFKKLRFSSKKTMSLSQRLYEGMDIGNGQRNGLITYMRTDSVR
HCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCH
LSSEFVERARSWILSELGETFASPLERKIRKSAKKIQDAHEAIRVTDPFLTPKEIKKFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
KEEAALYDLIWKRTISSLLPAEEFIKIEYSIFAAGECFQLETKKTIFPGYKILNEADVKT
CHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEECCCEEEECCCCCCCCCHHHHCCCCCCC
NPSWEKGELFILQKVECEKKQTEPPSRYSEGTLVAKLEKQGIGRPSTYATVSETLLKRKY
CCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHHH
VYEEKKFFYPFSLGEKVNFFLQSSFGELFREKFTAELESDLDRIEKKEIDSNSILNRLWL
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLQTQIQNSKFILFQKEWATVLQKKKETGWGICPVCRNGILQKKKSSRKKEFYQCNRFPD
HHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
CEFVSYELPESLE
CCEEEECCCCCCC
>Mature Secondary Structure 
SLLIIVESPSKAKTIAGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVILPGKK
EEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCEEEECCHH
KILSEIIKTAKQSQKVFLATDPDREGEFISAYIRDRLQKKSNVFRIRFTEVTKQAILNSL
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
QNPDTINEFLVDAQKTRRIGDRLIGYFISPVLWKQIGPGLSAGRVQSVALKWICEREEEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RNFKIEIYYNILLHGTDQKGIVGIFSRTGDRIFSKEKADQILQNVQKEKELRISEKKETL
CCEEEEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLFPPPPFQTASLQQEAFKKLRFSSKKTMSLSQRLYEGMDIGNGQRNGLITYMRTDSVR
HCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCH
LSSEFVERARSWILSELGETFASPLERKIRKSAKKIQDAHEAIRVTDPFLTPKEIKKFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
KEEAALYDLIWKRTISSLLPAEEFIKIEYSIFAAGECFQLETKKTIFPGYKILNEADVKT
CHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEECCCEEEECCCCCCCCCHHHHCCCCCCC
NPSWEKGELFILQKVECEKKQTEPPSRYSEGTLVAKLEKQGIGRPSTYATVSETLLKRKY
CCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHHH
VYEEKKFFYPFSLGEKVNFFLQSSFGELFREKFTAELESDLDRIEKKEIDSNSILNRLWL
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLQTQIQNSKFILFQKEWATVLQKKKETGWGICPVCRNGILQKKKSSRKKEFYQCNRFPD
HHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
CEFVSYELPESLE
CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]