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The map label for this gene is cca [H]

Identifier: 45657793

GI number: 45657793

Start: 2336072

End: 2337370

Strand: Direct

Name: cca [H]

Synonym: LIC11934

Alternate gene names: 45657793

Gene position: 2336072-2337370 (Clockwise)

Preceding gene: 45657792

Following gene: 45657794

Centisome position: 54.62

GC content: 34.26

Gene sequence:

>1299_bases
ATGACTAACGTAGATCCAGTTTTATTGATTCAAAAAATTCCTAAAATTTTTCTGGAAGATATACTTCATATTACACAAAT
CATTCGCAAAGAAGGCGGAGAATGTTATTTAGTAGGAGGTTCGGTACGAGATTTAGCACTTTCTAAAATTCCAAAAGAAT
TTGATCTTACTACTTCTCTTTTACCTGAGAAAATTCTTTCCTTATTTAAAAGAACCATTCCCACTGGAATCAAACATGGA
ACGGTAACCGTTCTGATACAAGATCGCGCTTACGAAATTACAACCTTTCGAAAAGACGCAGACTACTTAGACGGAAGAAG
GCCGGAGATTGTAGAATTTGGAGTTTCATTAAGTGAAGATTTAAAACGAAGAGATTTCACTATGAATGCGCTCGCTTTTG
ATTTAGAAACGAAAGCTCTCATAGACGAACATTCCGGTCTCTTGGATATTCAAAATAAAATCATCCGTACAATCGGAAAT
CCAATCGAAAGATTTACGGAAGACGGACTTAGACCAATTCGAGCGATTCGTTTTGTAAGTAGTCTGGGTTTTCATTTAGA
ACCGAACACAGCAAAGGCGATCATTCTTTGCAAAAACGTAACCGCAAAGGTTTCCAGAGAAAGAGTTCATGACGAACTGA
ATAAAATTCTAAAAAGTAAAAATCCTACTCCTTCTTTAAAATTATTTAAGGAATTTGGAATTTTAGAATTGTTCACCAAA
CTCAAACTCTATCCTGTAAAAAATGAAATCGTAGAAAATAAAATCCAAGAAGTTCCAGCGTTTCCTCTTAGTCTCAGACT
TTCTTTTTTACAAGCTTGGTTATTCGGGAATTTTCAAAACCGAAATTCTGCAAAACAATTTATGAAAGATCTACGTTATT
CCAATCAAAACACAAAAGAATCAATCTTTTTCTCGGATACTATTTCCGTTTTGTTGAACCATAAAAACGATTCTACACTA
ACCGACTCGGAAATCAGAAAAATTTTATTACATCCCGTTTGTGTTTATTCCGGGAGAGAAAATCTTAAAATTGTAATTGA
ACACATTCTGAATCTTGCATATATCTATGAACCAACTCTAAAATCTGTTTTCAATTTTCATAAAATATTAGATCTATTAT
TACAACCACAAGCTCTTTTAGTTACCGAGTTAGCTCTTAGAGGCGAGGATATTCTTGAAGTTTGTCCAAATCTCCCTCCC
AAAGAAATCGGTTCTGTTCTTGGAAAACTCCTTTCTTTTGTGCTCGAAAATCCGGAAGCAAATCGAAAAGATTCTTTGAT
TCCTCTACTTCCAAAGTAA

Upstream 100 bases:

>100_bases
ACAGTAGATCCAGAATGGAAAAAGAAAATCGATACCATTCTTGAAAACGACCAGACTCTAAAAAATAGATATGGAAATCT
CTGGAAAAAAATGATTCAAA

Downstream 100 bases:

>100_bases
ATAACGCAAGTTCGGTAGAAAGAATTTTCTAAAAGTATGAAATTCCTACAGTTTTAGAATCTGTTCTTACTCGAAACTGC
ATTATAAAGAGCCTAATCCA

Product: polynucleotide adenyltransferase-like protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNVDPVLLIQKIPKIFLEDILHITQIIRKEGGECYLVGGSVRDLALSKIPKEFDLTTSLLPEKILSLFKRTIPTGIKHG
TVTVLIQDRAYEITTFRKDADYLDGRRPEIVEFGVSLSEDLKRRDFTMNALAFDLETKALIDEHSGLLDIQNKIIRTIGN
PIERFTEDGLRPIRAIRFVSSLGFHLEPNTAKAIILCKNVTAKVSRERVHDELNKILKSKNPTPSLKLFKEFGILELFTK
LKLYPVKNEIVENKIQEVPAFPLSLRLSFLQAWLFGNFQNRNSAKQFMKDLRYSNQNTKESIFFSDTISVLLNHKNDSTL
TDSEIRKILLHPVCVYSGRENLKIVIEHILNLAYIYEPTLKSVFNFHKILDLLLQPQALLVTELALRGEDILEVCPNLPP
KEIGSVLGKLLSFVLENPEANRKDSLIPLLPK

Sequences:

>Translated_432_residues
MTNVDPVLLIQKIPKIFLEDILHITQIIRKEGGECYLVGGSVRDLALSKIPKEFDLTTSLLPEKILSLFKRTIPTGIKHG
TVTVLIQDRAYEITTFRKDADYLDGRRPEIVEFGVSLSEDLKRRDFTMNALAFDLETKALIDEHSGLLDIQNKIIRTIGN
PIERFTEDGLRPIRAIRFVSSLGFHLEPNTAKAIILCKNVTAKVSRERVHDELNKILKSKNPTPSLKLFKEFGILELFTK
LKLYPVKNEIVENKIQEVPAFPLSLRLSFLQAWLFGNFQNRNSAKQFMKDLRYSNQNTKESIFFSDTISVLLNHKNDSTL
TDSEIRKILLHPVCVYSGRENLKIVIEHILNLAYIYEPTLKSVFNFHKILDLLLQPQALLVTELALRGEDILEVCPNLPP
KEIGSVLGKLLSFVLENPEANRKDSLIPLLPK
>Mature_431_residues
TNVDPVLLIQKIPKIFLEDILHITQIIRKEGGECYLVGGSVRDLALSKIPKEFDLTTSLLPEKILSLFKRTIPTGIKHGT
VTVLIQDRAYEITTFRKDADYLDGRRPEIVEFGVSLSEDLKRRDFTMNALAFDLETKALIDEHSGLLDIQNKIIRTIGNP
IERFTEDGLRPIRAIRFVSSLGFHLEPNTAKAIILCKNVTAKVSRERVHDELNKILKSKNPTPSLKLFKEFGILELFTKL
KLYPVKNEIVENKIQEVPAFPLSLRLSFLQAWLFGNFQNRNSAKQFMKDLRYSNQNTKESIFFSDTISVLLNHKNDSTLT
DSEIRKILLHPVCVYSGRENLKIVIEHILNLAYIYEPTLKSVFNFHKILDLLLQPQALLVTELALRGEDILEVCPNLPPK
EIGSVLGKLLSFVLENPEANRKDSLIPLLPK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=213, Percent_Identity=36.6197183098592, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081691, Length=241, Percent_Identity=31.9502074688797, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789436, Length=220, Percent_Identity=30, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=243, Percent_Identity=31.2757201646091, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=32.7102803738318, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=32.7102803738318, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 49260; Mature: 49129

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVDPVLLIQKIPKIFLEDILHITQIIRKEGGECYLVGGSVRDLALSKIPKEFDLTTSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCHHCHHHHH
LPEKILSLFKRTIPTGIKHGTVTVLIQDRAYEITTFRKDADYLDGRRPEIVEFGVSLSED
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCHHHCCCCCCCHHHHCCCHHHH
LKRRDFTMNALAFDLETKALIDEHSGLLDIQNKIIRTIGNPIERFTEDGLRPIRAIRFVS
HHHHCCCHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLGFHLEPNTAKAIILCKNVTAKVSRERVHDELNKILKSKNPTPSLKLFKEFGILELFTK
HCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LKLYPVKNEIVENKIQEVPAFPLSLRLSFLQAWLFGNFQNRNSAKQFMKDLRYSNQNTKE
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCH
SIFFSDTISVLLNHKNDSTLTDSEIRKILLHPVCVYSGRENLKIVIEHILNLAYIYEPTL
HEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KSVFNFHKILDLLLQPQALLVTELALRGEDILEVCPNLPPKEIGSVLGKLLSFVLENPEA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NRKDSLIPLLPK
CCCCCCCCCCCC
>Mature Secondary Structure 
TNVDPVLLIQKIPKIFLEDILHITQIIRKEGGECYLVGGSVRDLALSKIPKEFDLTTSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCHHCHHHHH
LPEKILSLFKRTIPTGIKHGTVTVLIQDRAYEITTFRKDADYLDGRRPEIVEFGVSLSED
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCHHHCCCCCCCHHHHCCCHHHH
LKRRDFTMNALAFDLETKALIDEHSGLLDIQNKIIRTIGNPIERFTEDGLRPIRAIRFVS
HHHHCCCHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLGFHLEPNTAKAIILCKNVTAKVSRERVHDELNKILKSKNPTPSLKLFKEFGILELFTK
HCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LKLYPVKNEIVENKIQEVPAFPLSLRLSFLQAWLFGNFQNRNSAKQFMKDLRYSNQNTKE
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCH
SIFFSDTISVLLNHKNDSTLTDSEIRKILLHPVCVYSGRENLKIVIEHILNLAYIYEPTL
HEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KSVFNFHKILDLLLQPQALLVTELALRGEDILEVCPNLPPKEIGSVLGKLLSFVLENPEA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NRKDSLIPLLPK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]