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The map label for this gene is mmsB

Identifier: 45657723

GI number: 45657723

Start: 2254954

End: 2255844

Strand: Direct

Name: mmsB

Synonym: LIC11860

Alternate gene names: 45657723

Gene position: 2254954-2255844 (Clockwise)

Preceding gene: 45657722

Following gene: 45657733

Centisome position: 52.72

GC content: 36.36

Gene sequence:

>891_bases
ATGATTAATAAATATACTATATCCATCATAGGCACCGGAATTATGGGTCGTGGAATGGCGGTAAATCTTGCAAAAGCCGG
ACATAGTCTCAGACTCTACACAAGAAACCTTTCTAAAATCCAAGATTTAAAAAAAGATAACGTGCAAATTTTTGATTCTC
CCGTAGAAGCCGCTAAAAATTCTGATTTAGTAGTTCTTTGTCTGACCGAAGATCAAATCGTAGAAAAGGAAACGATTTCT
TCAGGACTTTTAGATACAAAACCTCCCATCTTAATCGATTGTGGAACAACTTCTCTTTCTTTGACACTAAAACTTTCAAA
GTTATGCTCGGAGAAAAAAATTCGTTTTTATGATTCTCCTATGACCGGTTCTAAAAACGCCGCAAGAGACGGACAGATTC
TTTTTATGATAGGCGCCAAACAAGTAGATATAAAAGACATTCAATTCTTCTTTGAAGTTTGTGGTAAAAACGCAGTCTAT
TGTGGTTCCATTGGAGGAGGTCAAAAGGCAAAACTTGCACTTAACATGATCCAGGCGGGAATTTTTCAAGTTTATATGGA
AGGTTTTGAACTTGCAAAAAATTCCGAGTTAGATCCTTCAATACTAAAAGAAATTCTTCTTCAATCCGCTGCTAAATCGG
GAATTGCAGAATTCAAATTTCCTTATGTATTTTCAGGAGACTACGAAACTCATTTTTCTCTCAAAAACATGAGAAAGGAC
GTTTATCACGCAATAGAACTTGCCAAAGAAAACAAAACAAACCTTTCCCTTTGTAAAAATCTTCCGGAAATCTACGACTT
AGGAATGAACGCTGGTTATGCGGAAAATGACTTTTGTAGTTTAAACGAAGTCACAGCGAAGATCCGTCCTCCCAAATCAG
AGAATCTGTAA

Upstream 100 bases:

>100_bases
TAAGATCTGTACCAAGACTGGGATAAATCTTACAGCTTAATTTTTCTGACAAATTTAATTAAAACTTTAAATACACCATA
AATTATTTTAATGTTATATT

Downstream 100 bases:

>100_bases
TCGCTTTTCCTTTTGAGTCTACTTTTAAACGAATGTGTACTTTCTCTTTTCTAAGTCTCTTTTCATATTCCAGAGCCTTT
GCCTCGTTCACATAAAAACG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MINKYTISIIGTGIMGRGMAVNLAKAGHSLRLYTRNLSKIQDLKKDNVQIFDSPVEAAKNSDLVVLCLTEDQIVEKETIS
SGLLDTKPPILIDCGTTSLSLTLKLSKLCSEKKIRFYDSPMTGSKNAARDGQILFMIGAKQVDIKDIQFFFEVCGKNAVY
CGSIGGGQKAKLALNMIQAGIFQVYMEGFELAKNSELDPSILKEILLQSAAKSGIAEFKFPYVFSGDYETHFSLKNMRKD
VYHAIELAKENKTNLSLCKNLPEIYDLGMNAGYAENDFCSLNEVTAKIRPPKSENL

Sequences:

>Translated_296_residues
MINKYTISIIGTGIMGRGMAVNLAKAGHSLRLYTRNLSKIQDLKKDNVQIFDSPVEAAKNSDLVVLCLTEDQIVEKETIS
SGLLDTKPPILIDCGTTSLSLTLKLSKLCSEKKIRFYDSPMTGSKNAARDGQILFMIGAKQVDIKDIQFFFEVCGKNAVY
CGSIGGGQKAKLALNMIQAGIFQVYMEGFELAKNSELDPSILKEILLQSAAKSGIAEFKFPYVFSGDYETHFSLKNMRKD
VYHAIELAKENKTNLSLCKNLPEIYDLGMNAGYAENDFCSLNEVTAKIRPPKSENL
>Mature_296_residues
MINKYTISIIGTGIMGRGMAVNLAKAGHSLRLYTRNLSKIQDLKKDNVQIFDSPVEAAKNSDLVVLCLTEDQIVEKETIS
SGLLDTKPPILIDCGTTSLSLTLKLSKLCSEKKIRFYDSPMTGSKNAARDGQILFMIGAKQVDIKDIQFFFEVCGKNAVY
CGSIGGGQKAKLALNMIQAGIFQVYMEGFELAKNSELDPSILKEILLQSAAKSGIAEFKFPYVFSGDYETHFSLKNMRKD
VYHAIELAKENKTNLSLCKNLPEIYDLGMNAGYAENDFCSLNEVTAKIRPPKSENL

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=23.9285714285714, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1786719, Length=278, Percent_Identity=24.8201438848921, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI145693186, Length=278, Percent_Identity=24.1007194244604, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790315, Length=297, Percent_Identity=23.5690235690236, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557316, Length=207, Percent_Identity=26.5700483091787, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=29.7872340425532, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=29.7872340425532, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=19.3548387096774, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32695; Mature: 32695

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKYTISIIGTGIMGRGMAVNLAKAGHSLRLYTRNLSKIQDLKKDNVQIFDSPVEAAKN
CCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHCCCCEEEEECHHHHCCC
SDLVVLCLTEDQIVEKETISSGLLDTKPPILIDCGTTSLSLTLKLSKLCSEKKIRFYDSP
CCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEHHHCCCCCEEEECCC
MTGSKNAARDGQILFMIGAKQVDIKDIQFFFEVCGKNAVYCGSIGGGQKAKLALNMIQAG
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
IFQVYMEGFELAKNSELDPSILKEILLQSAAKSGIAEFKFPYVFSGDYETHFSLKNMRKD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHCCCCEEEECCCCEEHHHHHHHHH
VYHAIELAKENKTNLSLCKNLPEIYDLGMNAGYAENDFCSLNEVTAKIRPPKSENL
HHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCCC
>Mature Secondary Structure
MINKYTISIIGTGIMGRGMAVNLAKAGHSLRLYTRNLSKIQDLKKDNVQIFDSPVEAAKN
CCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHCCCCEEEEECHHHHCCC
SDLVVLCLTEDQIVEKETISSGLLDTKPPILIDCGTTSLSLTLKLSKLCSEKKIRFYDSP
CCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEHHHCCCCCEEEECCC
MTGSKNAARDGQILFMIGAKQVDIKDIQFFFEVCGKNAVYCGSIGGGQKAKLALNMIQAG
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
IFQVYMEGFELAKNSELDPSILKEILLQSAAKSGIAEFKFPYVFSGDYETHFSLKNMRKD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHCCCCEEEECCCCEEHHHHHHHHH
VYHAIELAKENKTNLSLCKNLPEIYDLGMNAGYAENDFCSLNEVTAKIRPPKSENL
HHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA