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The map label for this gene is sppA

Identifier: 45657659

GI number: 45657659

Start: 2186279

End: 2187265

Strand: Direct

Name: sppA

Synonym: LIC11793

Alternate gene names: 45657659

Gene position: 2186279-2187265 (Clockwise)

Preceding gene: 45657653

Following gene: 45657660

Centisome position: 51.11

GC content: 35.87

Gene sequence:

>987_bases
TTGAAACACTCGATTCTAATTTGGACCCAAATTCTTTTTCTAAATCTTTTTTTTTCTAATTGTTACGTTCCAGTAAATGC
GAATTTATTTCCCTCAAAAACAGTGGAATTAAGGGAGAAACTTATTTCCGGTCAAAATGAAGATAAGATTCTAATCATAC
CTATAGACGGAGTGATCAGTGACGAAGGTGAAAAAAACTTTTTTGGAGGCCAAGAAGATTCTATCTTAGTCGAAGTCAAA
AAACAACTAGAACTCGCAGAATTAGATCCGGAAATCAAAGCAGTAATTTTGAAAATCAATTCTCCAGGCGGTTCTGTTAC
TGCAAGCGACATCATTTACAGAGAAATCCTTCAATTTAAAACCAAAAAGAAAATTCCTGTAGTTTCTCTTTTTATGGATA
CTGCTGCCAGCGGCGCCTATTATATTTCTATGGCTACCGATCTTTTGATCGCTCACCCTACTTCTGTGACCGGTTCTATC
GGTGTAATTCTTTCTGGAATCAACGTCAAAGAAGGTTTAGATAAACTTGGAATCAAAGATCAGTCGATTCGTTCTGGTGG
AAATAAAACCATTGGTTCTCCATTGGAAGAACTTTCCCCGGAGCAAAGAAAATTACTTCAATCCATTGTAGATGATCTTT
TTGAAAAATTCTTTGATATTGTCAAAAAAGGAAGACCTGGAAAAAATCCGTCGGAACTTCGTAAAGTTGCAGATGGCAGA
ATTTTCACAGCGTCCCAAGCGCTTCAACACGGTTTAATTGATAAAATAGGTTACTTTGACAACGCAGTACAAGAGACGAT
GGCTCTTTCGAATTACAAAAAAAATCCGGGAAATACAAATCCTAGAATCATTTATTATACTCGTAGTACCAACCAAAGAA
CAAATTTTTACCAAGTACAGTCCACTGAACTCAAACCGGATTCTACTATTTCTAAAATTCTAGGAACGAGTAGACAAGTT
CGTTTTTTATATCTTTGGTCTTACTAA

Upstream 100 bases:

>100_bases
TTGTAAAAACGGCTTTTCTAGACACAAAATTTAAGAACTGGAAAGGAATTGAAAACCTATTTGATTCGAGGAAACTGACG
ATTCCACAGGAGAATTTCTT

Downstream 100 bases:

>100_bases
GTAAACATTAGAAATTTAGGAATCTATTAATATCAATGTTATTCAATTCCCTACCCTTTTTATTTTTATTTCTGATCACG
TATTTGATCTACTGGAATGT

Product: endopeptidase IV

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKHSILIWTQILFLNLFFSNCYVPVNANLFPSKTVELREKLISGQNEDKILIIPIDGVISDEGEKNFFGGQEDSILVEVK
KQLELAELDPEIKAVILKINSPGGSVTASDIIYREILQFKTKKKIPVVSLFMDTAASGAYYISMATDLLIAHPTSVTGSI
GVILSGINVKEGLDKLGIKDQSIRSGGNKTIGSPLEELSPEQRKLLQSIVDDLFEKFFDIVKKGRPGKNPSELRKVADGR
IFTASQALQHGLIDKIGYFDNAVQETMALSNYKKNPGNTNPRIIYYTRSTNQRTNFYQVQSTELKPDSTISKILGTSRQV
RFLYLWSY

Sequences:

>Translated_328_residues
MKHSILIWTQILFLNLFFSNCYVPVNANLFPSKTVELREKLISGQNEDKILIIPIDGVISDEGEKNFFGGQEDSILVEVK
KQLELAELDPEIKAVILKINSPGGSVTASDIIYREILQFKTKKKIPVVSLFMDTAASGAYYISMATDLLIAHPTSVTGSI
GVILSGINVKEGLDKLGIKDQSIRSGGNKTIGSPLEELSPEQRKLLQSIVDDLFEKFFDIVKKGRPGKNPSELRKVADGR
IFTASQALQHGLIDKIGYFDNAVQETMALSNYKKNPGNTNPRIIYYTRSTNQRTNFYQVQSTELKPDSTISKILGTSRQV
RFLYLWSY
>Mature_328_residues
MKHSILIWTQILFLNLFFSNCYVPVNANLFPSKTVELREKLISGQNEDKILIIPIDGVISDEGEKNFFGGQEDSILVEVK
KQLELAELDPEIKAVILKINSPGGSVTASDIIYREILQFKTKKKIPVVSLFMDTAASGAYYISMATDLLIAHPTSVTGSI
GVILSGINVKEGLDKLGIKDQSIRSGGNKTIGSPLEELSPEQRKLLQSIVDDLFEKFFDIVKKGRPGKNPSELRKVADGR
IFTASQALQHGLIDKIGYFDNAVQETMALSNYKKNPGNTNPRIIYYTRSTNQRTNFYQVQSTELKPDSTISKILGTSRQV
RFLYLWSY

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=234, Percent_Identity=32.4786324786325, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787527, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 36645; Mature: 36645

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSILIWTQILFLNLFFSNCYVPVNANLFPSKTVELREKLISGQNEDKILIIPIDGVIS
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEC
DEGEKNFFGGQEDSILVEVKKQLELAELDPEIKAVILKINSPGGSVTASDIIYREILQFK
CCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
TKKKIPVVSLFMDTAASGAYYISMATDLLIAHPTSVTGSIGVILSGINVKEGLDKLGIKD
HCCCCCEEEEEHHHCCCCEEEEEEEHHEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCC
QSIRSGGNKTIGSPLEELSPEQRKLLQSIVDDLFEKFFDIVKKGRPGKNPSELRKVADGR
HHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
IFTASQALQHGLIDKIGYFDNAVQETMALSNYKKNPGNTNPRIIYYTRSTNQRTNFYQVQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEE
STELKPDSTISKILGTSRQVRFLYLWSY
CCCCCCHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MKHSILIWTQILFLNLFFSNCYVPVNANLFPSKTVELREKLISGQNEDKILIIPIDGVIS
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEC
DEGEKNFFGGQEDSILVEVKKQLELAELDPEIKAVILKINSPGGSVTASDIIYREILQFK
CCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
TKKKIPVVSLFMDTAASGAYYISMATDLLIAHPTSVTGSIGVILSGINVKEGLDKLGIKD
HCCCCCEEEEEHHHCCCCEEEEEEEHHEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCC
QSIRSGGNKTIGSPLEELSPEQRKLLQSIVDDLFEKFFDIVKKGRPGKNPSELRKVADGR
HHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
IFTASQALQHGLIDKIGYFDNAVQETMALSNYKKNPGNTNPRIIYYTRSTNQRTNFYQVQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEE
STELKPDSTISKILGTSRQVRFLYLWSY
CCCCCCHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]