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The map label for this gene is bioB

Identifier: 45657643

GI number: 45657643

Start: 2168865

End: 2169971

Strand: Reverse

Name: bioB

Synonym: LIC11777

Alternate gene names: 45657643

Gene position: 2169971-2168865 (Counterclockwise)

Preceding gene: 45657644

Following gene: 304570510

Centisome position: 50.73

GC content: 35.95

Gene sequence:

>1107_bases
TTGGGAATTAGACTTTTCAAGAAAACTCATTTGCTTGAATCTGTCTGCAGAATGCTCAAGACATCCGAAAAAATATTCTC
CGAAACCCCTAGTATAATTACAAAAGAAGAAGGTTTAAAAATTCTAAACGGTGTCATTCCTTTAACTACCTGTTTAGATA
AAGCGTTTCAAGAAAGAAATCGTTACTTCGAAAATAAAGTAAGAATTCATATTTTAGATAATATTAAAAATGGTTATTGT
CCGGAAGACTGTGGTTATTGTGCCCAAAGAAAAAACGCAAACTCAGGCGTTCAGGAATACCCTATGAAATCCGAACAAGA
GATTTATGAGGATGCTGTTCAGGCTAAAAAAAACGGAGCTTATCGTTTTTGTATGGTAACTTCTGGAACAGGGCCGAATC
GTTTAACAACTGAAAAACTTGCTTCTACCATTCAAAGAATTACTGACGAACTCAACATGAAGGTTTGTCTTTCTGCGGGT
TTGTTAGATATAGAAAAAGCTCAAGTTTTAAAAGAAGCTGGTCTAGATCGTTACAATCATAACCTCAATACTTCTGAAAA
TCATTATTCGGAAATCTGCGATACTCATACTTATTTACAAAGAGCTCAAACATTAGACTCGGTTTCTAAGGCTGGAATCG
GAATGTGTAGTGGAGTGATTGTGGGAATGGGGGAGTCTTTTCAAGATATTGTGGATGTTGCTTTTCAACTAAAATCTTTT
CGTGTAATTTCAATTCCAGTGAATTTTTTCATTCCAGTGAAAGGGCATACAATTAAAAATCCAAGCGTTTTAACTCCGGA
ACTTTGTGTAAGAATTCTTTGTATGTTTCGATTGATAAATCCGGATTCTGAAATTCGAATTGCGGCTGGAAGAGAAGGAC
ATCTTAGAAGCCTTTCCGCAACAGCTTTGTTTGCCGCTAATTCCTTATTCTCTTCCGGTTATTTAAACGTAAAGGGGTCG
GAGATTTTAGAAACGGTTGCAATGATCCGGGACGCCGGTTTTGTTCCTGAATTATCCAATGGGGAAATTCTTCCAGAAAA
TTTCGGCACGGAATCATTTTATTCTGAGAAAAATTTTCCCGAACTCTATAAATTTAAGAAATTTTAA

Upstream 100 bases:

>100_bases
TTGGGAAACGTAATTTATCTTACTCCTCCATACAATATAGAAAATTCTTCTTTGGAAAAAATCTTTTCCGCGATCCGAGA
AACCCTCTCTGAAATTTCTT

Downstream 100 bases:

>100_bases
AGATTTAAAAAATTTCAAAAAAAGTGGATTTTAATATTTTTTAAGCTTTCCCAGCAACTTTTTTTTATTAAATTGAGTCT
GAGAACAATGTGGATTGTTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MGIRLFKKTHLLESVCRMLKTSEKIFSETPSIITKEEGLKILNGVIPLTTCLDKAFQERNRYFENKVRIHILDNIKNGYC
PEDCGYCAQRKNANSGVQEYPMKSEQEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTEKLASTIQRITDELNMKVCLSAG
LLDIEKAQVLKEAGLDRYNHNLNTSENHYSEICDTHTYLQRAQTLDSVSKAGIGMCSGVIVGMGESFQDIVDVAFQLKSF
RVISIPVNFFIPVKGHTIKNPSVLTPELCVRILCMFRLINPDSEIRIAAGREGHLRSLSATALFAANSLFSSGYLNVKGS
EILETVAMIRDAGFVPELSNGEILPENFGTESFYSEKNFPELYKFKKF

Sequences:

>Translated_368_residues
MGIRLFKKTHLLESVCRMLKTSEKIFSETPSIITKEEGLKILNGVIPLTTCLDKAFQERNRYFENKVRIHILDNIKNGYC
PEDCGYCAQRKNANSGVQEYPMKSEQEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTEKLASTIQRITDELNMKVCLSAG
LLDIEKAQVLKEAGLDRYNHNLNTSENHYSEICDTHTYLQRAQTLDSVSKAGIGMCSGVIVGMGESFQDIVDVAFQLKSF
RVISIPVNFFIPVKGHTIKNPSVLTPELCVRILCMFRLINPDSEIRIAAGREGHLRSLSATALFAANSLFSSGYLNVKGS
EILETVAMIRDAGFVPELSNGEILPENFGTESFYSEKNFPELYKFKKF
>Mature_367_residues
GIRLFKKTHLLESVCRMLKTSEKIFSETPSIITKEEGLKILNGVIPLTTCLDKAFQERNRYFENKVRIHILDNIKNGYCP
EDCGYCAQRKNANSGVQEYPMKSEQEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTEKLASTIQRITDELNMKVCLSAGL
LDIEKAQVLKEAGLDRYNHNLNTSENHYSEICDTHTYLQRAQTLDSVSKAGIGMCSGVIVGMGESFQDIVDVAFQLKSFR
VISIPVNFFIPVKGHTIKNPSVLTPELCVRILCMFRLINPDSEIRIAAGREGHLRSLSATALFAANSLFSSGYLNVKGSE
ILETVAMIRDAGFVPELSNGEILPENFGTESFYSEKNFPELYKFKKF

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=296, Percent_Identity=33.1081081081081, Blast_Score=163, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321725, Length=250, Percent_Identity=34, Blast_Score=152, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 41265; Mature: 41134

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIRLFKKTHLLESVCRMLKTSEKIFSETPSIITKEEGLKILNGVIPLTTCLDKAFQERN
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RYFENKVRIHILDNIKNGYCPEDCGYCAQRKNANSGVQEYPMKSEQEIYEDAVQAKKNGA
HHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCC
YRFCMVTSGTGPNRLTTEKLASTIQRITDELNMKVCLSAGLLDIEKAQVLKEAGLDRYNH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCHHHCC
NLNTSENHYSEICDTHTYLQRAQTLDSVSKAGIGMCSGVIVGMGESFQDIVDVAFQLKSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCE
RVISIPVNFFIPVKGHTIKNPSVLTPELCVRILCMFRLINPDSEIRIAAGREGHLRSLSA
EEEEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
TALFAANSLFSSGYLNVKGSEILETVAMIRDAGFVPELSNGEILPENFGTESFYSEKNFP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCH
ELYKFKKF
HHHHHCCC
>Mature Secondary Structure 
GIRLFKKTHLLESVCRMLKTSEKIFSETPSIITKEEGLKILNGVIPLTTCLDKAFQERN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RYFENKVRIHILDNIKNGYCPEDCGYCAQRKNANSGVQEYPMKSEQEIYEDAVQAKKNGA
HHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCC
YRFCMVTSGTGPNRLTTEKLASTIQRITDELNMKVCLSAGLLDIEKAQVLKEAGLDRYNH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCHHHCC
NLNTSENHYSEICDTHTYLQRAQTLDSVSKAGIGMCSGVIVGMGESFQDIVDVAFQLKSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCE
RVISIPVNFFIPVKGHTIKNPSVLTPELCVRILCMFRLINPDSEIRIAAGREGHLRSLSA
EEEEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
TALFAANSLFSSGYLNVKGSEILETVAMIRDAGFVPELSNGEILPENFGTESFYSEKNFP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCH
ELYKFKKF
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA