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The map label for this gene is pmbA

Identifier: 45657606

GI number: 45657606

Start: 2124746

End: 2126080

Strand: Reverse

Name: pmbA

Synonym: LIC11740

Alternate gene names: 45657606

Gene position: 2126080-2124746 (Counterclockwise)

Preceding gene: 45657607

Following gene: 45657605

Centisome position: 49.71

GC content: 37.45

Gene sequence:

>1335_bases
ATGAATCTGGAACATTCCGTAGAATACGTGTTAGACGTTTGTAAAAAGAAAGGTTTAGACGGTTTTGATTTAGTTGCTTC
CGAGTCTAAAGATGTAGGAATTGAACTTTTTAGAAAAAGGGTCAGCAATACTGAACTTTCCAATTCCAGAGGGATCGGAA
TTCGTTTGATCAGGTCTGGAAAACCTGGATATTCCTACAGCGAAAAACTATCAGAAGAAGCTTTGTCTCAAATGGTAGAA
GACGCAATTTCTCAGTCTAAAATTTCAGATCCTCTTGATATTGATTTACCTGGTCCTACTGAACTTCCTAAAATAGAAAT
TCGTTCTTATGAGGAATCTTTAGAATCCTTAGGTTTTGAATGGCTGAGATCTACTGGAGAGAAGTTAGACGATTTGGCTT
GGTCAGTAGGTGGTAAAATTGAAAACGTTCCCTATTCATATGCGGGAAAAACCTGGAGTCGATTTATATTAGCAAACTCG
AATGATCTTTATCATAGTGAAAAATCGAACCTAGTTTCTGCGGGTGTTGCGTTAGTCGCAACAGACGGTAAGACCAAAAA
AATGGGAGGTTACACTCGTTCCGGTTTAGATCTGGATAAAATAAACCCAGAGCTCATTGTGTCTACTGCTACAGAACGTT
CTTTAGCCCTGTTAGGCGCAAAACCAGTAAAAAGTGGTTCTTATCCAATTCTACTTTCTAACCGTATTAGTCCTCAAATT
TTTGGAATGTTTTCTTCTCCATTTTCCGCAGATAGCGTTCAAAAAGGTCTTTCTAGACTAGATGGAAAAGTAGGTTCTCA
AATCGCATCACAAAATTGGAATGTGTTTTGCGATCCACATATTAGTGACTATCCAGGTTCCAGACTCTTAGACGCGGAAG
GAGTTTTGACCAGAAAAAAAGCAATTATCGAAAACGGAATTCTAAAAACCTATTTATACAATTTAGAATCTGCTAAAAAG
GCTGGTGTCTCTCCTACCGGTAACGCGGTTCGTTCTTATGGTGGAAGGGTGGGTACTTCGTTTAACAATTATGTGGTCCC
AAAGGGAAATCAAACTTTGGAAGAATTATTAAAAAGTGTTCCCGAATGTATTTATATTTTAAAATTAGAAGGTGGTTCCG
GTTGTAGCGCTGTATCCGGAGAAATTTCCATCGGGGTTCAAGGAATTTATTATAAAAATGGGAATCCAGTTCATCCAGTA
GATAATATCACTATGAATCTGAATTTTTTTGATCTACTGTTTAGGATTGATGGAATTTCCAATGAATACAACGATTCTTA
TTCTTCGATTAAGATTCCGGATATTTTAATTCGTGAGGCAAGTATAGCCGGTTAA

Upstream 100 bases:

>100_bases
AACTAGCAGCTGGAATGTGTGGTGCGGCTTCCGGTTCTGTTCCGGTTACGGTGGGGCAACCTTCTTTAAAAGTGGACGAG
ATTCTGGTAGGAGGTCGTTC

Downstream 100 bases:

>100_bases
AGTTTTCGCTTGCCTCCTCTTTAGGTTTAGTGTTTTATTTCTGAATTATATCTTTCTTCTCGGGAGAAACCCTTTGAACC
ATCTTTTTCAAACTAAATTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNLEHSVEYVLDVCKKKGLDGFDLVASESKDVGIELFRKRVSNTELSNSRGIGIRLIRSGKPGYSYSEKLSEEALSQMVE
DAISQSKISDPLDIDLPGPTELPKIEIRSYEESLESLGFEWLRSTGEKLDDLAWSVGGKIENVPYSYAGKTWSRFILANS
NDLYHSEKSNLVSAGVALVATDGKTKKMGGYTRSGLDLDKINPELIVSTATERSLALLGAKPVKSGSYPILLSNRISPQI
FGMFSSPFSADSVQKGLSRLDGKVGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTRKKAIIENGILKTYLYNLESAKK
AGVSPTGNAVRSYGGRVGTSFNNYVVPKGNQTLEELLKSVPECIYILKLEGGSGCSAVSGEISIGVQGIYYKNGNPVHPV
DNITMNLNFFDLLFRIDGISNEYNDSYSSIKIPDILIREASIAG

Sequences:

>Translated_444_residues
MNLEHSVEYVLDVCKKKGLDGFDLVASESKDVGIELFRKRVSNTELSNSRGIGIRLIRSGKPGYSYSEKLSEEALSQMVE
DAISQSKISDPLDIDLPGPTELPKIEIRSYEESLESLGFEWLRSTGEKLDDLAWSVGGKIENVPYSYAGKTWSRFILANS
NDLYHSEKSNLVSAGVALVATDGKTKKMGGYTRSGLDLDKINPELIVSTATERSLALLGAKPVKSGSYPILLSNRISPQI
FGMFSSPFSADSVQKGLSRLDGKVGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTRKKAIIENGILKTYLYNLESAKK
AGVSPTGNAVRSYGGRVGTSFNNYVVPKGNQTLEELLKSVPECIYILKLEGGSGCSAVSGEISIGVQGIYYKNGNPVHPV
DNITMNLNFFDLLFRIDGISNEYNDSYSSIKIPDILIREASIAG
>Mature_444_residues
MNLEHSVEYVLDVCKKKGLDGFDLVASESKDVGIELFRKRVSNTELSNSRGIGIRLIRSGKPGYSYSEKLSEEALSQMVE
DAISQSKISDPLDIDLPGPTELPKIEIRSYEESLESLGFEWLRSTGEKLDDLAWSVGGKIENVPYSYAGKTWSRFILANS
NDLYHSEKSNLVSAGVALVATDGKTKKMGGYTRSGLDLDKINPELIVSTATERSLALLGAKPVKSGSYPILLSNRISPQI
FGMFSSPFSADSVQKGLSRLDGKVGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTRKKAIIENGILKTYLYNLESAKK
AGVSPTGNAVRSYGGRVGTSFNNYVVPKGNQTLEELLKSVPECIYILKLEGGSGCSAVSGEISIGVQGIYYKNGNPVHPV
DNITMNLNFFDLLFRIDGISNEYNDSYSSIKIPDILIREASIAG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=461, Percent_Identity=24.0780911062907, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789640, Length=386, Percent_Identity=25.3886010362694, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48370; Mature: 48370

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEHSVEYVLDVCKKKGLDGFDLVASESKDVGIELFRKRVSNTELSNSRGIGIRLIRSG
CCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC
KPGYSYSEKLSEEALSQMVEDAISQSKISDPLDIDLPGPTELPKIEIRSYEESLESLGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHH
WLRSTGEKLDDLAWSVGGKIENVPYSYAGKTWSRFILANSNDLYHSEKSNLVSAGVALVA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCCHHHCCEEEEE
TDGKTKKMGGYTRSGLDLDKINPELIVSTATERSLALLGAKPVKSGSYPILLSNRISPQI
ECCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCE
FGMFSSPFSADSVQKGLSRLDGKVGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTRKK
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCHHHHHH
AIIENGILKTYLYNLESAKKAGVSPTGNAVRSYGGRVGTSFNNYVVPKGNQTLEELLKSV
HHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHC
PECIYILKLEGGSGCSAVSGEISIGVQGIYYKNGNPVHPVDNITMNLNFFDLLFRIDGIS
CCEEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHCCCC
NEYNDSYSSIKIPDILIREASIAG
CCCCCCCCCEECCHHEEECCCCCC
>Mature Secondary Structure
MNLEHSVEYVLDVCKKKGLDGFDLVASESKDVGIELFRKRVSNTELSNSRGIGIRLIRSG
CCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC
KPGYSYSEKLSEEALSQMVEDAISQSKISDPLDIDLPGPTELPKIEIRSYEESLESLGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHH
WLRSTGEKLDDLAWSVGGKIENVPYSYAGKTWSRFILANSNDLYHSEKSNLVSAGVALVA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCCHHHCCEEEEE
TDGKTKKMGGYTRSGLDLDKINPELIVSTATERSLALLGAKPVKSGSYPILLSNRISPQI
ECCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCE
FGMFSSPFSADSVQKGLSRLDGKVGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTRKK
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCHHHHHH
AIIENGILKTYLYNLESAKKAGVSPTGNAVRSYGGRVGTSFNNYVVPKGNQTLEELLKSV
HHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHC
PECIYILKLEGGSGCSAVSGEISIGVQGIYYKNGNPVHPVDNITMNLNFFDLLFRIDGIS
CCEEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHCCCC
NEYNDSYSSIKIPDILIREASIAG
CCCCCCCCCEECCHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]