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The map label for this gene is dnaG

Identifier: 45657568

GI number: 45657568

Start: 2069283

End: 2071079

Strand: Reverse

Name: dnaG

Synonym: LIC11702

Alternate gene names: 45657568

Gene position: 2071079-2069283 (Counterclockwise)

Preceding gene: 45657569

Following gene: 45657567

Centisome position: 48.42

GC content: 37.62

Gene sequence:

>1797_bases
TTGTCCAACAAAAAGGATTTCATCGATCGAATTCACCGGGAAGTTCCCATCGAGTCCTATATATCTCGTTTTATTCCTCT
TAAAAAACGTGGCAAAAATTTTATAGGTCTTTGTCCATTTCACCAAGAAAAATCTCCTTCCTTCAACGTTTCCGCAGAAA
AACAATTTTATTACTGTTTTGGATGTAAGGCATCCGGAGATCTCATACGTTTTGTAATGAGTTACGAACGAGTAGATTTT
TCCAGATCTCTGGAAATTCTCTCCGAATATTCTGGAATCCCACTTGAAGAAAAAAGTTCTAAAAATTCTGAATTTTCAGA
TTTTCTTTATAAAATTAATTTAAAGGTTTCTGAATATTATCAACATCTGTTACATACCCCCACAGGTAAAAATGCACTTG
ATTATCTAAAATCTAGGGAAATAGAAGACTCAGAAATTCGTTTATTCGGTTTAGGTTATGCCCCTGAAGGTTTTGATACA
CTCGCCAAAGAAGTGTTAAAGACTAAGGAAGAAATCTCTGGAGCAATTCAACTAGGACTTCTCAGAGAAAAAGAAGCTGG
TAACGGACGTCCTTACGACTTTTTCCGAGATCGAATCATGTTTCCGGTATTGGATCTTTCTGGCAGAACGGTAGCATTCT
CAGGAAGAATTTTAGGTCCGGGAAAAGAAGCAAAGTATATCAATAGCCAGGCCTCGTTGATCTTTGATAAAAGTAGAACC
TTTTATAATTTCTTTAAGGCCAGAGAAGGGGTCCGTAAGTCTGGAGAGGCTATGCTCGTCGAAGGTTATTTAGACGTAAT
CGGATTGGTTAGAAGAGGTTACGAAAACGTTATCGCTTCTATGGGAACTGCAATTACAGAAAATCATATTCGAACTCTTA
AAAAATTTGCGGAAAGGGTTACTTTTGTTTTAGATGGGGATATAGCTGGTAAGAAAGGTGCGTTAAGAGCCGCGGAAATC
TGTCTAAAAGAAGGAATGGAATGCTCCATTGTACTTCTTCCAGAAGGAAAAGACCCTTTTGATCTGTCCAAATCACTTAG
CAGACCAGAATTACACGAAATACTTTCTGATCGGATTCAGGGTTCCGAATTTGTTGTAGAAGAGTTGCTGGAAAACGCAG
ATTCTCGTGCCCTACCAGAAAAAAAAAGAAAGTCACTTCAAAATTTATATTCCTTCATCCAAACCCTAGGCCGGGAAACG
GATAAACAATTCTTACTCGGATTGGGTGCAAACAAACTCGGAATTAGTATGGATGCAGTCCTTAGAGATTTTAAAGGTGG
AACTGGTGCAAAGAATGGACCTTCCAATGCCGATACTAATTCTAACTTAAAGGAAGTTCAGGGAATCTCTGGTCCGGCCT
TAGATTGTGAAAGAAAGATCGTTTCCATGCTCGTAAAACATACTGGGTTGTTTTCATATTCGGAAGAAATTTCTTCGATG
GAATTCATGGACATTGCAAGTTCTTATCTTTGGGATTATTTATACACAATTTACACGGGAGAAGAAGAAATTTCTCCGGT
AGGAATTTTAACCTCCGAACTTCCGGAGGATTTAAAACAAATTCTGGCTCCTTATCTTTTAGAAGAAGATTCTGAAAAAA
CGGAACCGGGAGAACTACATAAGGTCTTTAGAATTCTTTTGTTACAACAAAAGAAGTTTAGAATCGAGGAAAAAATTCGG
GAACTCGATCAAAAAAGAGAACGTTTTTTTACACCCGAAATTTTTACGGAACTGAGTTTTTACCGTAAAGAAAAAGAAAA
GATTCTGGAACATATCCGGAGTCAATCAACCACATAA

Upstream 100 bases:

>100_bases
GGGGCGGGTTATGGCTGCGTTCAAAGGAAAAAGTATCGACGGTACTAAAGTTTCGGCAATCGTTAAATCCAGGCTTTCCT
AAGTACTAAGTGAGCCCCCT

Downstream 100 bases:

>100_bases
ATTTAGAGGGGTTCGAATGGAAAATCTACAAAGTATGCCCGAGGTTCAAAAAATTATTTCCCTCGGAAAAGCAAACGGTG
AAGTTTCTTACGATGATATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSNKKDFIDRIHREVPIESYISRFIPLKKRGKNFIGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMSYERVDF
SRSLEILSEYSGIPLEEKSSKNSEFSDFLYKINLKVSEYYQHLLHTPTGKNALDYLKSREIEDSEIRLFGLGYAPEGFDT
LAKEVLKTKEEISGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTVAFSGRILGPGKEAKYINSQASLIFDKSRT
FYNFFKAREGVRKSGEAMLVEGYLDVIGLVRRGYENVIASMGTAITENHIRTLKKFAERVTFVLDGDIAGKKGALRAAEI
CLKEGMECSIVLLPEGKDPFDLSKSLSRPELHEILSDRIQGSEFVVEELLENADSRALPEKKRKSLQNLYSFIQTLGRET
DKQFLLGLGANKLGISMDAVLRDFKGGTGAKNGPSNADTNSNLKEVQGISGPALDCERKIVSMLVKHTGLFSYSEEISSM
EFMDIASSYLWDYLYTIYTGEEEISPVGILTSELPEDLKQILAPYLLEEDSEKTEPGELHKVFRILLLQQKKFRIEEKIR
ELDQKRERFFTPEIFTELSFYRKEKEKILEHIRSQSTT

Sequences:

>Translated_598_residues
MSNKKDFIDRIHREVPIESYISRFIPLKKRGKNFIGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMSYERVDF
SRSLEILSEYSGIPLEEKSSKNSEFSDFLYKINLKVSEYYQHLLHTPTGKNALDYLKSREIEDSEIRLFGLGYAPEGFDT
LAKEVLKTKEEISGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTVAFSGRILGPGKEAKYINSQASLIFDKSRT
FYNFFKAREGVRKSGEAMLVEGYLDVIGLVRRGYENVIASMGTAITENHIRTLKKFAERVTFVLDGDIAGKKGALRAAEI
CLKEGMECSIVLLPEGKDPFDLSKSLSRPELHEILSDRIQGSEFVVEELLENADSRALPEKKRKSLQNLYSFIQTLGRET
DKQFLLGLGANKLGISMDAVLRDFKGGTGAKNGPSNADTNSNLKEVQGISGPALDCERKIVSMLVKHTGLFSYSEEISSM
EFMDIASSYLWDYLYTIYTGEEEISPVGILTSELPEDLKQILAPYLLEEDSEKTEPGELHKVFRILLLQQKKFRIEEKIR
ELDQKRERFFTPEIFTELSFYRKEKEKILEHIRSQSTT
>Mature_597_residues
SNKKDFIDRIHREVPIESYISRFIPLKKRGKNFIGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMSYERVDFS
RSLEILSEYSGIPLEEKSSKNSEFSDFLYKINLKVSEYYQHLLHTPTGKNALDYLKSREIEDSEIRLFGLGYAPEGFDTL
AKEVLKTKEEISGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTVAFSGRILGPGKEAKYINSQASLIFDKSRTF
YNFFKAREGVRKSGEAMLVEGYLDVIGLVRRGYENVIASMGTAITENHIRTLKKFAERVTFVLDGDIAGKKGALRAAEIC
LKEGMECSIVLLPEGKDPFDLSKSLSRPELHEILSDRIQGSEFVVEELLENADSRALPEKKRKSLQNLYSFIQTLGRETD
KQFLLGLGANKLGISMDAVLRDFKGGTGAKNGPSNADTNSNLKEVQGISGPALDCERKIVSMLVKHTGLFSYSEEISSME
FMDIASSYLWDYLYTIYTGEEEISPVGILTSELPEDLKQILAPYLLEEDSEKTEPGELHKVFRILLLQQKKFRIEEKIRE
LDQKRERFFTPEIFTELSFYRKEKEKILEHIRSQSTT

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=482, Percent_Identity=32.1576763485477, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68194; Mature: 68063

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKDFIDRIHREVPIESYISRFIPLKKRGKNFIGLCPFHQEKSPSFNVSAEKQFYYCF
CCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEE
GCKASGDLIRFVMSYERVDFSRSLEILSEYSGIPLEEKSSKNSEFSDFLYKINLKVSEYY
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCHHHHH
QHLLHTPTGKNALDYLKSREIEDSEIRLFGLGYAPEGFDTLAKEVLKTKEEISGAIQLGL
HHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LREKEAGNGRPYDFFRDRIMFPVLDLSGRTVAFSGRILGPGKEAKYINSQASLIFDKSRT
HCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEECEEECCCCCHHHHCCCHHEEEECCHH
FYNFFKAREGVRKSGEAMLVEGYLDVIGLVRRGYENVIASMGTAITENHIRTLKKFAERV
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVLDGDIAGKKGALRAAEICLKEGMECSIVLLPEGKDPFDLSKSLSRPELHEILSDRIQ
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHCC
GSEFVVEELLENADSRALPEKKRKSLQNLYSFIQTLGRETDKQFLLGLGANKLGISMDAV
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHCCHHHHH
LRDFKGGTGAKNGPSNADTNSNLKEVQGISGPALDCERKIVSMLVKHTGLFSYSEEISSM
HHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
EFMDIASSYLWDYLYTIYTGEEEISPVGILTSELPEDLKQILAPYLLEEDSEKTEPGELH
HHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KVFRILLLQQKKFRIEEKIRELDQKRERFFTPEIFTELSFYRKEKEKILEHIRSQSTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNKKDFIDRIHREVPIESYISRFIPLKKRGKNFIGLCPFHQEKSPSFNVSAEKQFYYCF
CCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEE
GCKASGDLIRFVMSYERVDFSRSLEILSEYSGIPLEEKSSKNSEFSDFLYKINLKVSEYY
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCHHHHH
QHLLHTPTGKNALDYLKSREIEDSEIRLFGLGYAPEGFDTLAKEVLKTKEEISGAIQLGL
HHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LREKEAGNGRPYDFFRDRIMFPVLDLSGRTVAFSGRILGPGKEAKYINSQASLIFDKSRT
HCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEECEEECCCCCHHHHCCCHHEEEECCHH
FYNFFKAREGVRKSGEAMLVEGYLDVIGLVRRGYENVIASMGTAITENHIRTLKKFAERV
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVLDGDIAGKKGALRAAEICLKEGMECSIVLLPEGKDPFDLSKSLSRPELHEILSDRIQ
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHCC
GSEFVVEELLENADSRALPEKKRKSLQNLYSFIQTLGRETDKQFLLGLGANKLGISMDAV
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHCCHHHHH
LRDFKGGTGAKNGPSNADTNSNLKEVQGISGPALDCERKIVSMLVKHTGLFSYSEEISSM
HHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
EFMDIASSYLWDYLYTIYTGEEEISPVGILTSELPEDLKQILAPYLLEEDSEKTEPGELH
HHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KVFRILLLQQKKFRIEEKIRELDQKRERFFTPEIFTELSFYRKEKEKILEHIRSQSTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]