Click here to switch to the map view.

The map label for this gene is exoA [H]

Identifier: 45657327

GI number: 45657327

Start: 1789376

End: 1790140

Strand: Reverse

Name: exoA [H]

Synonym: LIC11452

Alternate gene names: 45657327

Gene position: 1790140-1789376 (Counterclockwise)

Preceding gene: 45657338

Following gene: 45657326

Centisome position: 41.85

GC content: 39.87

Gene sequence:

>765_bases
ATGAAATTTATCTCTCTCAACTGTAATGGAATTCGTTCTTCCCTAGAAAAAGGACTGGCCGATTATATTCGAAATACAAA
ACCGGATTTTATCTGTTTTCAAGAAACAAAGGCAAACCAAGACCAGGTTCCCCCTTCTCTCTGGGAAGAAGGAGGTTATA
CTCCGGTTTTCCACAGCGCCGAAAAAAAAGGATACAGCGGAGTTGCCGTTCTTTATAAAAAACCCCCGGAAAAAATCACA
ATCGGAATCGGAGATCCGTTTTTTGACAAAGAAGGAAGAAGTATCTATTTAGAATATCCGAACTTCGCGCTTTGGAATTT
ATACTTTCCTTCCGGAACCACAGGAGACATACGTCAAGCGGCAAAGATGAAGTTTCTAGACTTATTTCAAAAAGAATCTT
CTAAAAGGAGAAAAAAACAACCGAATATCATAGTATGTGGGGACGTAAACATTGCTCATACCCCTCAAGATATTCACGAT
CCAAAAGGAAACGCAAAGAGCAGCGGATTTTTACCCGAAGAAAGAGAATGGTTATCCGAATTTTTAAACAAGGGTTGGGT
AGATACATTTCGATATCTCTATCCAGACAAACAGGAATATTCCTGGTGGACGTTTCGCGCCGGAGCCAGGGCCAAAAATA
AGGGCTGGAGAATTGACTATTTTTTTGTAACAGAAGAACTTAAGAAAAACGTAAAGAGCCATTCCATCTTCAGAGACAAA
CCTTTTTCTGACCACGCCCCTCTTGAGTTTGAAATTAAACTTTAG

Upstream 100 bases:

>100_bases
TCTAAGAAACCAGATTTTCCAAAAGAACGAATCTTGCATCTGAAATTTATTCAAAAAACAATTGCTTATAAAAGGAATTC
CTCCAATTTACACTCTGCTT

Downstream 100 bases:

>100_bases
AAATTTATAATGAACTCTTTTTATTTTTTGAAGAAAGTTTATAAAGCAAAACCTAGAATAGAGTCTTTGAAAAATTAGTT
TTCTTCTATTTCTTATTTAT

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKFISLNCNGIRSSLEKGLADYIRNTKPDFICFQETKANQDQVPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPEKIT
IGIGDPFFDKEGRSIYLEYPNFALWNLYFPSGTTGDIRQAAKMKFLDLFQKESSKRRKKQPNIIVCGDVNIAHTPQDIHD
PKGNAKSSGFLPEEREWLSEFLNKGWVDTFRYLYPDKQEYSWWTFRAGARAKNKGWRIDYFFVTEELKKNVKSHSIFRDK
PFSDHAPLEFEIKL

Sequences:

>Translated_254_residues
MKFISLNCNGIRSSLEKGLADYIRNTKPDFICFQETKANQDQVPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPEKIT
IGIGDPFFDKEGRSIYLEYPNFALWNLYFPSGTTGDIRQAAKMKFLDLFQKESSKRRKKQPNIIVCGDVNIAHTPQDIHD
PKGNAKSSGFLPEEREWLSEFLNKGWVDTFRYLYPDKQEYSWWTFRAGARAKNKGWRIDYFFVTEELKKNVKSHSIFRDK
PFSDHAPLEFEIKL
>Mature_254_residues
MKFISLNCNGIRSSLEKGLADYIRNTKPDFICFQETKANQDQVPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPEKIT
IGIGDPFFDKEGRSIYLEYPNFALWNLYFPSGTTGDIRQAAKMKFLDLFQKESSKRRKKQPNIIVCGDVNIAHTPQDIHD
PKGNAKSSGFLPEEREWLSEFLNKGWVDTFRYLYPDKQEYSWWTFRAGARAKNKGWRIDYFFVTEELKKNVKSHSIFRDK
PFSDHAPLEFEIKL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=36.046511627907, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=36.046511627907, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=36.046511627907, Blast_Score=181, Evalue=7e-46,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=34.3283582089552, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=34.5098039215686, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221330655, Length=249, Percent_Identity=39.7590361445783, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17136678, Length=249, Percent_Identity=39.7590361445783, Blast_Score=181, Evalue=4e-46,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29516; Mature: 29516

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISLNCNGIRSSLEKGLADYIRNTKPDFICFQETKANQDQVPPSLWEEGGYTPVFHSA
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCEEECC
EKKGYSGVAVLYKKPPEKITIGIGDPFFDKEGRSIYLEYPNFALWNLYFPSGTTGDIRQA
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHH
AKMKFLDLFQKESSKRRKKQPNIIVCGDVNIAHTPQDIHDPKGNAKSSGFLPEEREWLSE
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHH
FLNKGWVDTFRYLYPDKQEYSWWTFRAGARAKNKGWRIDYFFVTEELKKNVKSHSIFRDK
HHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCC
PFSDHAPLEFEIKL
CCCCCCCEEEEEEC
>Mature Secondary Structure
MKFISLNCNGIRSSLEKGLADYIRNTKPDFICFQETKANQDQVPPSLWEEGGYTPVFHSA
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCEEECC
EKKGYSGVAVLYKKPPEKITIGIGDPFFDKEGRSIYLEYPNFALWNLYFPSGTTGDIRQA
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHH
AKMKFLDLFQKESSKRRKKQPNIIVCGDVNIAHTPQDIHDPKGNAKSSGFLPEEREWLSE
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHH
FLNKGWVDTFRYLYPDKQEYSWWTFRAGARAKNKGWRIDYFFVTEELKKNVKSHSIFRDK
HHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCC
PFSDHAPLEFEIKL
CCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]