Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 45656887

GI number: 45656887

Start: 1217077

End: 1218069

Strand: Reverse

Name: rlmL [H]

Synonym: LIC10999

Alternate gene names: 45656887

Gene position: 1218069-1217077 (Counterclockwise)

Preceding gene: 45656890

Following gene: 45656883

Centisome position: 28.48

GC content: 34.24

Gene sequence:

>993_bases
ATGTTGGAAAAACAATTTAATTCGTATAACGATTTTGGAAATCCTATGGTGATGTTTCGAAATCGTATAACCAGAATGGC
TAAACACTGGAAAAAATGGGCCCGTAAAAGAAACATAGAATGTTTTCGAATCTATGACAGAGATATACCTCAGGTTCCTG
TTTGCGTAGATCTTTATGGCCCCTTGTGTCATATCTCTGTGTATAAAAATAATTACGAAATTTCAGATGAAGATAGAGTG
AAAGAAAGTGAAGAAATCAGTAAAATCATTTGTGAAATTCTCTCTATCCATCCAAATCAAATTTTTTGGAAAAAAAGAGA
ACCTAAAAAAGGTAAGGAACAATACGAAAAACAATCCGAACAATCTGAGTTATTTGAGGTTGGAGAAAACGGACTTAGAT
TTTATGTGAATTTATCAGACTATGTAGATACAGGGCTGTTTTTAGATCATAGGATCACTAGAGATCTCGTTCGTAAAGAA
TCCAAGGGAAAAAAATTCTTAAATCTTTTTTCTTATACCGGATCATTTACCGTCTACGCGGCGTCAGGTGGAGCTACAAA
AAGCCTAAGCGTGGATCTTTCCAACACCTATTCCGACTGGGCCGAAGAAAATTTGAAACTGAACGGTTTTTCACTTACAA
AACACCGTATACTTAGAGCGGATGTAATGGAATGGCTTCGGCATGAAAGAAAAAACCCTGATCGAGAAAAATATGATCTG
ATTGTAGTCGACCCACCTACTTTTTCTAACAGTAAAAAAACAAACGAAATCTTTGATATACAAAAAAATCACGTTGAAGT
TCTAAATACACTTTATCGAGACTTTGCATTTCCTGGTGCCGTTTTGTTTTTTTCTACAAACTTTAGAAAATTTGAATTTT
CTAAACAGTCCGTTTTATGGGACAACTTGAAAGACATTTCAAAGTTTACAATTCCAGAAGATTTTAGAAATGAAAAAATC
CATTTTTGCTGGAGAATGGAAAAACCAGTTTGA

Upstream 100 bases:

>100_bases
CGCAACCATAGTTTACGATTTGTAAATTTACCAGTCTTTTTACGAAATTTAACTTTTTGTTCTGATTGGATTTTAGAATA
GATTTTTTAATTCTGGTCCC

Downstream 100 bases:

>100_bases
ATTCTATATTTCGGCAGAGTAGCAATTTTTGCACTTTAGAAAAATATGAAATAGAAACTTTGTCGTTTTCACTTTCTTCG
AAAACAAGTTTTAAAACTGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLEKQFNSYNDFGNPMVMFRNRITRMAKHWKKWARKRNIECFRIYDRDIPQVPVCVDLYGPLCHISVYKNNYEISDEDRV
KESEEISKIICEILSIHPNQIFWKKREPKKGKEQYEKQSEQSELFEVGENGLRFYVNLSDYVDTGLFLDHRITRDLVRKE
SKGKKFLNLFSYTGSFTVYAASGGATKSLSVDLSNTYSDWAEENLKLNGFSLTKHRILRADVMEWLRHERKNPDREKYDL
IVVDPPTFSNSKKTNEIFDIQKNHVEVLNTLYRDFAFPGAVLFFSTNFRKFEFSKQSVLWDNLKDISKFTIPEDFRNEKI
HFCWRMEKPV

Sequences:

>Translated_330_residues
MLEKQFNSYNDFGNPMVMFRNRITRMAKHWKKWARKRNIECFRIYDRDIPQVPVCVDLYGPLCHISVYKNNYEISDEDRV
KESEEISKIICEILSIHPNQIFWKKREPKKGKEQYEKQSEQSELFEVGENGLRFYVNLSDYVDTGLFLDHRITRDLVRKE
SKGKKFLNLFSYTGSFTVYAASGGATKSLSVDLSNTYSDWAEENLKLNGFSLTKHRILRADVMEWLRHERKNPDREKYDL
IVVDPPTFSNSKKTNEIFDIQKNHVEVLNTLYRDFAFPGAVLFFSTNFRKFEFSKQSVLWDNLKDISKFTIPEDFRNEKI
HFCWRMEKPV
>Mature_330_residues
MLEKQFNSYNDFGNPMVMFRNRITRMAKHWKKWARKRNIECFRIYDRDIPQVPVCVDLYGPLCHISVYKNNYEISDEDRV
KESEEISKIICEILSIHPNQIFWKKREPKKGKEQYEKQSEQSELFEVGENGLRFYVNLSDYVDTGLFLDHRITRDLVRKE
SKGKKFLNLFSYTGSFTVYAASGGATKSLSVDLSNTYSDWAEENLKLNGFSLTKHRILRADVMEWLRHERKNPDREKYDL
IVVDPPTFSNSKKTNEIFDIQKNHVEVLNTLYRDFAFPGAVLFFSTNFRKFEFSKQSVLWDNLKDISKFTIPEDFRNEKI
HFCWRMEKPV

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=309, Percent_Identity=43.042071197411, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI145693111, Length=290, Percent_Identity=26.8965517241379, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 39320; Mature: 39320

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQFNSYNDFGNPMVMFRNRITRMAKHWKKWARKRNIECFRIYDRDIPQVPVCVDLYG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCC
PLCHISVYKNNYEISDEDRVKESEEISKIICEILSIHPNQIFWKKREPKKGKEQYEKQSE
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
QSELFEVGENGLRFYVNLSDYVDTGLFLDHRITRDLVRKESKGKKFLNLFSYTGSFTVYA
HHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEE
ASGGATKSLSVDLSNTYSDWAEENLKLNGFSLTKHRILRADVMEWLRHERKNPDREKYDL
ECCCCCEEEEEECCCCHHHHHHCCEEECCEEHHHHHHHHHHHHHHHHHHHCCCCCHHEEE
IVVDPPTFSNSKKTNEIFDIQKNHVEVLNTLYRDFAFPGAVLFFSTNFRKFEFSKQSVLW
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHH
DNLKDISKFTIPEDFRNEKIHFCWRMEKPV
HHHHHHHHCCCCHHHCCCEEEEEEEECCCC
>Mature Secondary Structure
MLEKQFNSYNDFGNPMVMFRNRITRMAKHWKKWARKRNIECFRIYDRDIPQVPVCVDLYG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCC
PLCHISVYKNNYEISDEDRVKESEEISKIICEILSIHPNQIFWKKREPKKGKEQYEKQSE
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
QSELFEVGENGLRFYVNLSDYVDTGLFLDHRITRDLVRKESKGKKFLNLFSYTGSFTVYA
HHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEE
ASGGATKSLSVDLSNTYSDWAEENLKLNGFSLTKHRILRADVMEWLRHERKNPDREKYDL
ECCCCCEEEEEECCCCHHHHHHCCEEECCEEHHHHHHHHHHHHHHHHHHHCCCCCHHEEE
IVVDPPTFSNSKKTNEIFDIQKNHVEVLNTLYRDFAFPGAVLFFSTNFRKFEFSKQSVLW
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHH
DNLKDISKFTIPEDFRNEKIHFCWRMEKPV
HHHHHHHHCCCCHHHCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA