Click here to switch to the map view.

The map label for this gene is amiA

Identifier: 45656635

GI number: 45656635

Start: 901162

End: 902253

Strand: Direct

Name: amiA

Synonym: LIC10739

Alternate gene names: 45656635

Gene position: 901162-902253 (Clockwise)

Preceding gene: 45656634

Following gene: 45656636

Centisome position: 21.07

GC content: 38.19

Gene sequence:

>1092_bases
TTGGCAAAAAATCAAATCTATCTTTGGGGGCTGATCTTATTTTTACTAAGTTTGTGGAATTTGGAAGCCAAAGTTTCCAT
TTCTACACAGTCTTCTAAACGTTATGTGCGCTTTGAAGATGTTCAAAGAGAATTTCCTTCTCTCAAATCTACGTTTAATC
CCGCGACTTTTGTTGGATCGATTCAACATCCTTCCGGAGAAGTTCGTTTTAGAGTAGGTTCTTCTTTTTATACGTTCAAT
CAAACTATAGAAAAAATTTCCGTTCCGATTCTTTATAAAGAAAAAGATTTTCTGATTCCTCCTGAAATTGTAGAAGCTCT
TTTTGTACAACTTATGCCAGAGGATGTTCGTTACGAATATAAGGAAAACGTGTTGGAGTTGGAAGTATTGCCTAGTGCCG
AAAAATTGGAAGTTAAGACAATTCTTATAGACGCTGGACATGGAGGAAAGGACCCAGGAACCTTGTCGAATGATGGAACC
AACGAAAAGTCAGTCGCTTTACAAGTGGCAAAGATTCTACAAAAATTTTTTGAAAAAGTATATCCTACGATCAACATAGT
ATTAACGAGGGCTGACGACACTTTTATAGAATTGGAACGTAGATCCGAGATTGCAAATCGGGAACTCAAAAAAAACGGAA
GCACTTTGTTTATCAGTCTTCATTGTAATTCGTCCATCAACGAAGAAGTGAACGGATTCGAGATCTATTATCTTTCACAA
ACCCCTTCTACAGAATCGGCAAGAGAAACCGCACTTTTAGAAAATAAAATTCTCAAAGCGAAGGGAAGTCCAGTCGTCAA
AAAAATTCAGGCCGGAATGATGTCTTCTTTGATTCAGAGAAGGAGTAGAATCTTGGCGAGATCTTTGGAATCGGAGATGA
AAAAAAAGCTCCAACCTCAAATCCTGTCCAGGGGAGTGAAAAAGGCGGATTTTTCAGTCCTGAGAGGAAGTCTTATGCCT
GCGGTTCTCGTAGAGATGGGGTATCTTTCTCATGAAAAAGAATCCAAACTTTTGCAGAGCAAGAGTTTACAAGTAAAGAT
AGCGAAAAGCATTGTAGAAGGAATCCGTGGATATGAATTGGCAAAACATTAA

Upstream 100 bases:

>100_bases
AAAATCAACAGTTCGGTAAACAATACGATAAAAACAAACGGAATCTATTCGATATAAACGATACGGTAAAAGAAGATACT
AAAAAGAAAAAGGTTTCGAT

Downstream 100 bases:

>100_bases
AGAATCTGCAAATACGATTAAAGATACAATTTGGGAAGCGGCCCTAAGAGCGGTAGAAAAAATCAATCAAGGATACCTTT
GGCTTTTTCGTACTGCGAGC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKNQIYLWGLILFLLSLWNLEAKVSISTQSSKRYVRFEDVQREFPSLKSTFNPATFVGSIQHPSGEVRFRVGSSFYTFN
QTIEKISVPILYKEKDFLIPPEIVEALFVQLMPEDVRYEYKENVLELEVLPSAEKLEVKTILIDAGHGGKDPGTLSNDGT
NEKSVALQVAKILQKFFEKVYPTINIVLTRADDTFIELERRSEIANRELKKNGSTLFISLHCNSSINEEVNGFEIYYLSQ
TPSTESARETALLENKILKAKGSPVVKKIQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMP
AVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKH

Sequences:

>Translated_363_residues
MAKNQIYLWGLILFLLSLWNLEAKVSISTQSSKRYVRFEDVQREFPSLKSTFNPATFVGSIQHPSGEVRFRVGSSFYTFN
QTIEKISVPILYKEKDFLIPPEIVEALFVQLMPEDVRYEYKENVLELEVLPSAEKLEVKTILIDAGHGGKDPGTLSNDGT
NEKSVALQVAKILQKFFEKVYPTINIVLTRADDTFIELERRSEIANRELKKNGSTLFISLHCNSSINEEVNGFEIYYLSQ
TPSTESARETALLENKILKAKGSPVVKKIQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMP
AVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKH
>Mature_362_residues
AKNQIYLWGLILFLLSLWNLEAKVSISTQSSKRYVRFEDVQREFPSLKSTFNPATFVGSIQHPSGEVRFRVGSSFYTFNQ
TIEKISVPILYKEKDFLIPPEIVEALFVQLMPEDVRYEYKENVLELEVLPSAEKLEVKTILIDAGHGGKDPGTLSNDGTN
EKSVALQVAKILQKFFEKVYPTINIVLTRADDTFIELERRSEIANRELKKNGSTLFISLHCNSSINEEVNGFEIYYLSQT
PSTESARETALLENKILKAKGSPVVKKIQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMPA
VLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKH

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=32.0512820512821, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790611, Length=232, Percent_Identity=30.6034482758621, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082163, Length=256, Percent_Identity=28.125, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41095; Mature: 40964

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNQIYLWGLILFLLSLWNLEAKVSISTQSSKRYVRFEDVQREFPSLKSTFNPATFVGS
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCHHEEEE
IQHPSGEVRFRVGSSFYTFNQTIEKISVPILYKEKDFLIPPEIVEALFVQLMPEDVRYEY
CCCCCCCEEEEECCEEEHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHH
KENVLELEVLPSAEKLEVKTILIDAGHGGKDPGTLSNDGTNEKSVALQVAKILQKFFEKV
HCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YPTINIVLTRADDTFIELERRSEIANRELKKNGSTLFISLHCNSSINEEVNGFEIYYLSQ
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
TPSTESARETALLENKILKAKGSPVVKKIQAGMMSSLIQRRSRILARSLESEMKKKLQPQ
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ILSRGVKKADFSVLRGSLMPAVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYEL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
AKH
CCC
>Mature Secondary Structure 
AKNQIYLWGLILFLLSLWNLEAKVSISTQSSKRYVRFEDVQREFPSLKSTFNPATFVGS
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCHHEEEE
IQHPSGEVRFRVGSSFYTFNQTIEKISVPILYKEKDFLIPPEIVEALFVQLMPEDVRYEY
CCCCCCCEEEEECCEEEHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHH
KENVLELEVLPSAEKLEVKTILIDAGHGGKDPGTLSNDGTNEKSVALQVAKILQKFFEKV
HCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YPTINIVLTRADDTFIELERRSEIANRELKKNGSTLFISLHCNSSINEEVNGFEIYYLSQ
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
TPSTESARETALLENKILKAKGSPVVKKIQAGMMSSLIQRRSRILARSLESEMKKKLQPQ
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ILSRGVKKADFSVLRGSLMPAVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYEL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
AKH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]