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The map label for this gene is mnmC

Identifier: 45656546

GI number: 45656546

Start: 792350

End: 794293

Strand: Direct

Name: mnmC

Synonym: LIC10648

Alternate gene names: 45656546

Gene position: 792350-794293 (Clockwise)

Preceding gene: 45656545

Following gene: 45656547

Centisome position: 18.53

GC content: 35.65

Gene sequence:

>1944_bases
ATGCTCACTTGGAAAAATAATCTTACTCCTGTATCAGAACAATTTGATGATATTTATTTTTCTCCGGAAAATGGATTGGA
GGAAACCAAACACGTTTTTATTAAAGGGAATGATCTTTATAATAGATGGAGAAATTGGAATATTCAGAATGCGTTTTGTA
TCTTAGAATTGGGTTTTGGCACAGGGCTTAATTTTTTAACCACCTGGAAAGAATACTTAGAATACAAAGATCGGTTTAGA
CTACATTTTATATCCATAGAAAAATTCCCACTCAATCGAGAGGAGATTTCTAAAGCGCTTTCCATATTTTCGGAACTTGT
GGAAATTAAAAAAGAATTTCTATCCTCATACCAAGATTTAATTCCAGGAATGAATTATTTTCAATTTTTAGGAGGGAAAA
TTCATTTTTCTCTTTTTTTGGGTGATGTTTCAAGTGCGTTATGCGAAATATCTGGAAAAGTGGATGCTATTTTTTTGGAC
GGTTTTGCTCCTTCTAAAAATCCTGAGATGTGGGATAAGTCCGTTTTAGAAAACTTAAAATACGTTTCCAAAAAAGGAAC
CACGTTGTCCACATTTACAGTAGCAAGAATGGTTAGGGATTCGTTATCTTTCTCCGGGTTTAAGTTGGAAAAACGCCCTG
GTTTTGGAAGGAAAAGAGAGATGTTGATCGGAAGTTATTCCGATTCTTTTTTAGAATCCAATCCAAAAGAAAAACCTTGG
TGTAAACGTGCTTATCCGGAATTACAAATCAAAACCGTTGCGATTGTAGGAGCGGGAATTGCGGGAACCACTCTCGCTTA
TTCCTTATCAAGGCGAGGAATACAAGTTTTTCTAATCGATCCTTCCGGGATTGCAAAGGAAACTTCTGGAATTCCGATGG
CTATTTCTCATCCTCATCTCACTAAAATTCCCGGACCTATTAGTCTATTCACGTTACGCGCCTTTAGATACGCCCTTTCC
TTTCTTTCTTCATTTGCAGATCAGAACTTTTTTGAAAAAACTGGATTATTTCACAGTGTTACACAAGAGATGGATTCGGA
AAGACTTCAAAAGGGTATAGAAAATCATAAACTTTCCGAAGAGATTGTATTTTGGAAACCGATCGCTTCTAAATTTCAGA
ACGGAGATTTTTTAGAAAACGAACCAGGAGTTTTTTTCCGAAATGGATTTTGGACTCGTCCTGAATCTATCGCAAAAAAA
TGCGCAGAACAACCGGGGGTCGAATTTATAAAGGGAACCGCAAGTCGTATAGAACAAGACGGGACTTCTTGGAAGTTACT
AATTCAAGAATCTGGACACGAAATAGTTGCAAATTCTATTATATTCTGTAATTCTCATTCGATTGGAAAGTTAATCGCTT
CTTTATTTGAAGGAGAAGAACCGTTTCCAATTCGAAAAGTTAGGGGACAACTTATATCTTTAAAAGAAACGGAGAAATCG
TCTCGTATCTCGAACATTTTATGCGCGGAACATTATTTGACTCCTTCTATTTTAGGAGAACATATTTTAGGTTCGACTTT
CGACGAATTTGATTTAAATCCGCTTCCTCAAAAAAAAGATACGGACCGGCTTTTAGAATTTGTTCAAAACAAATATCCAA
GTTTAAATTTTGATTCGAGTTGTGTGCTTATCGAAAAAGTAGGATTGAGGGCTCAAACTCCAGATCGTCTTCCAATTTTA
GGCCCAGTTTTTGATCCGAGAGAATTTAGAAAAATTTATAAAGAAATTGATTTACCTAAAAATAGAAACAAAAAATTTCC
AAATCTAAAAACGATTCAAGGGTTATATGTGTTTGGAGGTTTAGGTTCAAGAGGAATTTTAAGTTCTTTTTTAGGCTCTG
AAATTATGGCCTCTTTGATACTAGGAGAACCGGTTCCTGTTGAATCTTCCGTTTTAGAGTATCTACATCCAGCGAGATTT
CTCTACCGAAAAGTTCGTAAGTAA

Upstream 100 bases:

>100_bases
TATCCAGTTCCAACTCCGAAGATTTGTTTCGCAAAGAAAGTGGTCCAGGAAAAGAATCTTCTCCGCCAAAATCTACGGAA
CCCCTGCCTCCGAAAATCTA

Downstream 100 bases:

>100_bases
ATTCGGTTTGTGAAGAAAGAATATTAGATTTTTCTTTAGAATTGATCTCAAAAACCTATAAATGCGGGAACTCTCATAAT
AGCAAAAAAACGATTCGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MLTWKNNLTPVSEQFDDIYFSPENGLEETKHVFIKGNDLYNRWRNWNIQNAFCILELGFGTGLNFLTTWKEYLEYKDRFR
LHFISIEKFPLNREEISKALSIFSELVEIKKEFLSSYQDLIPGMNYFQFLGGKIHFSLFLGDVSSALCEISGKVDAIFLD
GFAPSKNPEMWDKSVLENLKYVSKKGTTLSTFTVARMVRDSLSFSGFKLEKRPGFGRKREMLIGSYSDSFLESNPKEKPW
CKRAYPELQIKTVAIVGAGIAGTTLAYSLSRRGIQVFLIDPSGIAKETSGIPMAISHPHLTKIPGPISLFTLRAFRYALS
FLSSFADQNFFEKTGLFHSVTQEMDSERLQKGIENHKLSEEIVFWKPIASKFQNGDFLENEPGVFFRNGFWTRPESIAKK
CAEQPGVEFIKGTASRIEQDGTSWKLLIQESGHEIVANSIIFCNSHSIGKLIASLFEGEEPFPIRKVRGQLISLKETEKS
SRISNILCAEHYLTPSILGEHILGSTFDEFDLNPLPQKKDTDRLLEFVQNKYPSLNFDSSCVLIEKVGLRAQTPDRLPIL
GPVFDPREFRKIYKEIDLPKNRNKKFPNLKTIQGLYVFGGLGSRGILSSFLGSEIMASLILGEPVPVESSVLEYLHPARF
LYRKVRK

Sequences:

>Translated_647_residues
MLTWKNNLTPVSEQFDDIYFSPENGLEETKHVFIKGNDLYNRWRNWNIQNAFCILELGFGTGLNFLTTWKEYLEYKDRFR
LHFISIEKFPLNREEISKALSIFSELVEIKKEFLSSYQDLIPGMNYFQFLGGKIHFSLFLGDVSSALCEISGKVDAIFLD
GFAPSKNPEMWDKSVLENLKYVSKKGTTLSTFTVARMVRDSLSFSGFKLEKRPGFGRKREMLIGSYSDSFLESNPKEKPW
CKRAYPELQIKTVAIVGAGIAGTTLAYSLSRRGIQVFLIDPSGIAKETSGIPMAISHPHLTKIPGPISLFTLRAFRYALS
FLSSFADQNFFEKTGLFHSVTQEMDSERLQKGIENHKLSEEIVFWKPIASKFQNGDFLENEPGVFFRNGFWTRPESIAKK
CAEQPGVEFIKGTASRIEQDGTSWKLLIQESGHEIVANSIIFCNSHSIGKLIASLFEGEEPFPIRKVRGQLISLKETEKS
SRISNILCAEHYLTPSILGEHILGSTFDEFDLNPLPQKKDTDRLLEFVQNKYPSLNFDSSCVLIEKVGLRAQTPDRLPIL
GPVFDPREFRKIYKEIDLPKNRNKKFPNLKTIQGLYVFGGLGSRGILSSFLGSEIMASLILGEPVPVESSVLEYLHPARF
LYRKVRK
>Mature_647_residues
MLTWKNNLTPVSEQFDDIYFSPENGLEETKHVFIKGNDLYNRWRNWNIQNAFCILELGFGTGLNFLTTWKEYLEYKDRFR
LHFISIEKFPLNREEISKALSIFSELVEIKKEFLSSYQDLIPGMNYFQFLGGKIHFSLFLGDVSSALCEISGKVDAIFLD
GFAPSKNPEMWDKSVLENLKYVSKKGTTLSTFTVARMVRDSLSFSGFKLEKRPGFGRKREMLIGSYSDSFLESNPKEKPW
CKRAYPELQIKTVAIVGAGIAGTTLAYSLSRRGIQVFLIDPSGIAKETSGIPMAISHPHLTKIPGPISLFTLRAFRYALS
FLSSFADQNFFEKTGLFHSVTQEMDSERLQKGIENHKLSEEIVFWKPIASKFQNGDFLENEPGVFFRNGFWTRPESIAKK
CAEQPGVEFIKGTASRIEQDGTSWKLLIQESGHEIVANSIIFCNSHSIGKLIASLFEGEEPFPIRKVRGQLISLKETEKS
SRISNILCAEHYLTPSILGEHILGSTFDEFDLNPLPQKKDTDRLLEFVQNKYPSLNFDSSCVLIEKVGLRAQTPDRLPIL
GPVFDPREFRKIYKEIDLPKNRNKKFPNLKTIQGLYVFGGLGSRGILSSFLGSEIMASLILGEPVPVESSVLEYLHPARF
LYRKVRK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=665, Percent_Identity=30.6766917293233, Blast_Score=281, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_LEPIC (Q72UK9)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000632.1
- ProteinModelPortal:   Q72UK9
- SMR:   Q72UK9
- GeneID:   2769581
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10648
- NMPDR:   fig|267671.1.peg.632
- HOGENOM:   HBG110595
- OMA:   VIGETGF
- ProtClustDB:   CLSK574883
- BioCyc:   LINT267671:LIC_10648-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 73613; Mature: 73613

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTWKNNLTPVSEQFDDIYFSPENGLEETKHVFIKGNDLYNRWRNWNIQNAFCILELGFG
CCCCCCCCCCHHHHCCCEEECCCCCCCCCCEEEEECCHHHHHHCCCCCCCEEEEEEECCC
TGLNFLTTWKEYLEYKDRFRLHFISIEKFPLNREEISKALSIFSELVEIKKEFLSSYQDL
CCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPGMNYFQFLGGKIHFSLFLGDVSSALCEISGKVDAIFLDGFAPSKNPEMWDKSVLENLK
HCCCHHHHHHCCEEEEEEEHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
YVSKKGTTLSTFTVARMVRDSLSFSGFKLEKRPGFGRKREMLIGSYSDSFLESNPKEKPW
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEECCCHHHHHCCCCCCCH
CKRAYPELQIKTVAIVGAGIAGTTLAYSLSRRGIQVFLIDPSGIAKETSGIPMAISHPHL
HHHCCCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCC
TKIPGPISLFTLRAFRYALSFLSSFADQNFFEKTGLFHSVTQEMDSERLQKGIENHKLSE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIVFWKPIASKFQNGDFLENEPGVFFRNGFWTRPESIAKKCAEQPGVEFIKGTASRIEQD
CEEEECHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GTSWKLLIQESGHEIVANSIIFCNSHSIGKLIASLFEGEEPFPIRKVRGQLISLKETEKS
CCCEEEEEECCCCEEEECCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHH
SRISNILCAEHYLTPSILGEHILGSTFDEFDLNPLPQKKDTDRLLEFVQNKYPSLNFDSS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
CVLIEKVGLRAQTPDRLPILGPVFDPREFRKIYKEIDLPKNRNKKFPNLKTIQGLYVFGG
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEEEECC
LGSRGILSSFLGSEIMASLILGEPVPVESSVLEYLHPARFLYRKVRK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTWKNNLTPVSEQFDDIYFSPENGLEETKHVFIKGNDLYNRWRNWNIQNAFCILELGFG
CCCCCCCCCCHHHHCCCEEECCCCCCCCCCEEEEECCHHHHHHCCCCCCCEEEEEEECCC
TGLNFLTTWKEYLEYKDRFRLHFISIEKFPLNREEISKALSIFSELVEIKKEFLSSYQDL
CCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPGMNYFQFLGGKIHFSLFLGDVSSALCEISGKVDAIFLDGFAPSKNPEMWDKSVLENLK
HCCCHHHHHHCCEEEEEEEHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
YVSKKGTTLSTFTVARMVRDSLSFSGFKLEKRPGFGRKREMLIGSYSDSFLESNPKEKPW
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEECCCHHHHHCCCCCCCH
CKRAYPELQIKTVAIVGAGIAGTTLAYSLSRRGIQVFLIDPSGIAKETSGIPMAISHPHL
HHHCCCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCC
TKIPGPISLFTLRAFRYALSFLSSFADQNFFEKTGLFHSVTQEMDSERLQKGIENHKLSE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIVFWKPIASKFQNGDFLENEPGVFFRNGFWTRPESIAKKCAEQPGVEFIKGTASRIEQD
CEEEECHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GTSWKLLIQESGHEIVANSIIFCNSHSIGKLIASLFEGEEPFPIRKVRGQLISLKETEKS
CCCEEEEEECCCCEEEECCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHH
SRISNILCAEHYLTPSILGEHILGSTFDEFDLNPLPQKKDTDRLLEFVQNKYPSLNFDSS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
CVLIEKVGLRAQTPDRLPILGPVFDPREFRKIYKEIDLPKNRNKKFPNLKTIQGLYVFGG
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEEEECC
LGSRGILSSFLGSEIMASLILGEPVPVESSVLEYLHPARFLYRKVRK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA