Click here to switch to the map view.

The map label for this gene is dus2 [H]

Identifier: 45656544

GI number: 45656544

Start: 790129

End: 791172

Strand: Reverse

Name: dus2 [H]

Synonym: LIC10646

Alternate gene names: 45656544

Gene position: 791172-790129 (Counterclockwise)

Preceding gene: 45656556

Following gene: 45656543

Centisome position: 18.5

GC content: 38.7

Gene sequence:

>1044_bases
ATGTTAGAGAAATACGGTAAAATTCCAAAACGATACCCGGTTTCGTTGGCTCCCATGATGGATTGGACCGATCGACATTT
TAGATATTTTTTAAGACTGATTTCTAAACATACGTTTTTATACACGGAAATGGTCCATACCGGAGCCGTACTTCACGGTG
ATCGTCGCAAACTTCTATCCTACAGCCCTGAAGAATTACCTCTTGCAATTCAACTAGGAGGAAGCGATCCGAAAGCACTT
GCAGAATCTTGTAAGATCGGAGAAGACTATGGATATACCGAAATCAATTTGAACGTAGGTTGTCCAAGTGATCGTGTAAG
AGAAGGAAACTTCGGCGCCTGTTTGATGGAAACTCCGGAAAAGGTTGCTGAATTGACGTTTGCAATGGACACTTCCGTTT
CAATTCCTATAACCGTAAAATGTAGAATTGGAATTCCAGGTAAGGAAACTTTTGAAGACCTATGTAAATTTATAGAATGT
GTTCGCCAAACGGGTGTTCAACGATTTATCGTTCATGCAAGAATCGCGATTTTAGGAGGACTTACCCCCGCACAGAATCG
TCAGGTTCCTCCTTTACATTATACGTACGTTGAATCTTTAAAGAAGGAATTTCCGGATCTTTTGATAGAAATCAATGGAG
GAATACGGACTATTACTTCAATTCGGGAAAGACTAGAAAAAAACGATGGAGTGATGATCGGAAGAGCCGCATACGAAACT
CCTTATCTCTTTTCGGAAGTAGATTCTTTGTTTTTTGGGGAAAATTTACCTCCTTTAAGTAGGAGAGAAATTTTGCTTAG
AATGAAGGATTATATAGAAGAAGTAATCTTAAAAGAGAAAAACGGAAAACCACACCACGCACTTAGACATATGTTAGGGT
TGTTTCACGGAGAAAAAGGAGCTCGCAGTTTTAGAAAAATTCTTACGGAAGGAATGTATTCCGGTTATTCGAAGGATCTT
TTATCCAAAGCCATTCAAGAAATTTCTAATGATTCTTTAGATATAGTTTCGTCTAACGCGTATCTGTATAAATCTGGTTC
GTAA

Upstream 100 bases:

>100_bases
TTGTTCTTAAATTTATTTTTTATTTCTTTAAAGTTTTTTATCATTGAGAGGGTTCTTGACTGAAGGGTTCGGAACAATAT
TCTGGTAATCCAATTCCTTT

Downstream 100 bases:

>100_bases
AACGGCTACTCTCCAAAGTTATCTTCAGAATAATAGAGTTATTGAAAAAATAATTCTCCATCTTGTTTCTGTTTTATGAA
ATAGTCAATTGAAGCAGTTT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLEKYGKIPKRYPVSLAPMMDWTDRHFRYFLRLISKHTFLYTEMVHTGAVLHGDRRKLLSYSPEELPLAIQLGGSDPKAL
AESCKIGEDYGYTEINLNVGCPSDRVREGNFGACLMETPEKVAELTFAMDTSVSIPITVKCRIGIPGKETFEDLCKFIEC
VRQTGVQRFIVHARIAILGGLTPAQNRQVPPLHYTYVESLKKEFPDLLIEINGGIRTITSIRERLEKNDGVMIGRAAYET
PYLFSEVDSLFFGENLPPLSRREILLRMKDYIEEVILKEKNGKPHHALRHMLGLFHGEKGARSFRKILTEGMYSGYSKDL
LSKAIQEISNDSLDIVSSNAYLYKSGS

Sequences:

>Translated_347_residues
MLEKYGKIPKRYPVSLAPMMDWTDRHFRYFLRLISKHTFLYTEMVHTGAVLHGDRRKLLSYSPEELPLAIQLGGSDPKAL
AESCKIGEDYGYTEINLNVGCPSDRVREGNFGACLMETPEKVAELTFAMDTSVSIPITVKCRIGIPGKETFEDLCKFIEC
VRQTGVQRFIVHARIAILGGLTPAQNRQVPPLHYTYVESLKKEFPDLLIEINGGIRTITSIRERLEKNDGVMIGRAAYET
PYLFSEVDSLFFGENLPPLSRREILLRMKDYIEEVILKEKNGKPHHALRHMLGLFHGEKGARSFRKILTEGMYSGYSKDL
LSKAIQEISNDSLDIVSSNAYLYKSGS
>Mature_347_residues
MLEKYGKIPKRYPVSLAPMMDWTDRHFRYFLRLISKHTFLYTEMVHTGAVLHGDRRKLLSYSPEELPLAIQLGGSDPKAL
AESCKIGEDYGYTEINLNVGCPSDRVREGNFGACLMETPEKVAELTFAMDTSVSIPITVKCRIGIPGKETFEDLCKFIEC
VRQTGVQRFIVHARIAILGGLTPAQNRQVPPLHYTYVESLKKEFPDLLIEINGGIRTITSIRERLEKNDGVMIGRAAYET
PYLFSEVDSLFFGENLPPLSRREILLRMKDYIEEVILKEKNGKPHHALRHMLGLFHGEKGARSFRKILTEGMYSGYSKDL
LSKAIQEISNDSLDIVSSNAYLYKSGS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=27.1186440677966, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI40807366, Length=236, Percent_Identity=24.5762711864407, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=48.7421383647799, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789660, Length=279, Percent_Identity=26.8817204301075, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788462, Length=271, Percent_Identity=26.9372693726937, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=168, Percent_Identity=29.1666666666667, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=290, Percent_Identity=25.5172413793103, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920448, Length=290, Percent_Identity=25.5172413793103, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 39332; Mature: 39332

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00559 MOLYBDOPTERIN_EUK ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKYGKIPKRYPVSLAPMMDWTDRHFRYFLRLISKHTFLYTEMVHTGAVLHGDRRKLLS
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCHHHHHC
YSPEELPLAIQLGGSDPKALAESCKIGEDYGYTEINLNVGCPSDRVREGNFGACLMETPE
CCCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCHH
KVAELTFAMDTSVSIPITVKCRIGIPGKETFEDLCKFIECVRQTGVQRFIVHARIAILGG
HHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPAQNRQVPPLHYTYVESLKKEFPDLLIEINGGIRTITSIRERLEKNDGVMIGRAAYET
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCC
PYLFSEVDSLFFGENLPPLSRREILLRMKDYIEEVILKEKNGKPHHALRHMLGLFHGEKG
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
ARSFRKILTEGMYSGYSKDLLSKAIQEISNDSLDIVSSNAYLYKSGS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCEEEEECCC
>Mature Secondary Structure
MLEKYGKIPKRYPVSLAPMMDWTDRHFRYFLRLISKHTFLYTEMVHTGAVLHGDRRKLLS
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCHHHHHC
YSPEELPLAIQLGGSDPKALAESCKIGEDYGYTEINLNVGCPSDRVREGNFGACLMETPE
CCCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCHH
KVAELTFAMDTSVSIPITVKCRIGIPGKETFEDLCKFIECVRQTGVQRFIVHARIAILGG
HHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPAQNRQVPPLHYTYVESLKKEFPDLLIEINGGIRTITSIRERLEKNDGVMIGRAAYET
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCC
PYLFSEVDSLFFGENLPPLSRREILLRMKDYIEEVILKEKNGKPHHALRHMLGLFHGEKG
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
ARSFRKILTEGMYSGYSKDLLSKAIQEISNDSLDIVSSNAYLYKSGS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]