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The map label for this gene is ispF

Identifier: 45656508

GI number: 45656508

Start: 748327

End: 748812

Strand: Direct

Name: ispF

Synonym: LIC10610

Alternate gene names: 45656508

Gene position: 748327-748812 (Clockwise)

Preceding gene: 45656507

Following gene: 45656509

Centisome position: 17.5

GC content: 35.6

Gene sequence:

>486_bases
ATGTATAGAATTGGAAACGGAATTGATTTTCATAAATTGGAAATCAATTTAAACAGGCCTTTGATATTGGGTGGGATAGA
GTGTGAATCAGAATTTGCACTTGTTGGACATTCGGATGCTGACATCATTTTACACGCGATTTCGGATGCAATTTTAGGAG
CACTTGCATTAGGCGATATTGGACAGTATTTTCCGGATACGGATCCAAGTTTGAAAAATATAGACAGTAAGATAATACTT
GTCAAATGTCTAGAGCTGATGAAGGAAAAAAATTTTGATCTTGTCAATATAGATTGTACCGTAATTGGAGAAAGACCTAA
GATCGCTCCACTCAAAGATAGAATTACAAAATCTTTAAGTAACTTATTGAATCTTCCTTTGGATTGTATTTCCGTAAAAG
CTACAACGACCGAAAAAATGGGCGCCTTGGGTAGACAGGAAGGAATCGGAACTTTTTGTTCGATACTTTTAGAAAAGAGA
TCTTGA

Upstream 100 bases:

>100_bases
ATAATTCTAATAATGATTTCTTGAAAATCTATCTCTCGAGCAAGATCTGTCTTTTTATAAAAAATTAGAAAAATAAAATA
AGTTAATTTAGGAATATTGA

Downstream 100 bases:

>100_bases
AATTTACCATTTGAAGATATGAAAATGATACCAAAAATTGGCGGTCGATTTTGTGAATATATAAACATTCTCAAAAATTG
CATTCAACTCGAAGTTATCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MYRIGNGIDFHKLEINLNRPLILGGIECESEFALVGHSDADIILHAISDAILGALALGDIGQYFPDTDPSLKNIDSKIIL
VKCLELMKEKNFDLVNIDCTVIGERPKIAPLKDRITKSLSNLLNLPLDCISVKATTTEKMGALGRQEGIGTFCSILLEKR
S

Sequences:

>Translated_161_residues
MYRIGNGIDFHKLEINLNRPLILGGIECESEFALVGHSDADIILHAISDAILGALALGDIGQYFPDTDPSLKNIDSKIIL
VKCLELMKEKNFDLVNIDCTVIGERPKIAPLKDRITKSLSNLLNLPLDCISVKATTTEKMGALGRQEGIGTFCSILLEKR
S
>Mature_161_residues
MYRIGNGIDFHKLEINLNRPLILGGIECESEFALVGHSDADIILHAISDAILGALALGDIGQYFPDTDPSLKNIDSKIIL
VKCLELMKEKNFDLVNIDCTVIGERPKIAPLKDRITKSLSNLLNLPLDCISVKATTTEKMGALGRQEGIGTFCSILLEKR
S

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=40.251572327044, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_LEPIC (Q72UP7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000594.1
- ProteinModelPortal:   Q72UP7
- SMR:   Q72UP7
- GeneID:   2771101
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10610
- NMPDR:   fig|267671.1.peg.594
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   LINT267671:LIC_10610-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17622; Mature: 17622

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIGNGIDFHKLEINLNRPLILGGIECESEFALVGHSDADIILHAISDAILGALALGDI
CCCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GQYFPDTDPSLKNIDSKIILVKCLELMKEKNFDLVNIDCTVIGERPKIAPLKDRITKSLS
HHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
NLLNLPLDCISVKATTTEKMGALGRQEGIGTFCSILLEKRS
HHHCCCHHHEEEECCCHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MYRIGNGIDFHKLEINLNRPLILGGIECESEFALVGHSDADIILHAISDAILGALALGDI
CCCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GQYFPDTDPSLKNIDSKIILVKCLELMKEKNFDLVNIDCTVIGERPKIAPLKDRITKSLS
HHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
NLLNLPLDCISVKATTTEKMGALGRQEGIGTFCSILLEKRS
HHHCCCHHHEEEECCCHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA