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The map label for this gene is ispE

Identifier: 45656326

GI number: 45656326

Start: 488013

End: 488906

Strand: Direct

Name: ispE

Synonym: LIC10426

Alternate gene names: 45656326

Gene position: 488013-488906 (Clockwise)

Preceding gene: 45656325

Following gene: 45656327

Centisome position: 11.41

GC content: 37.81

Gene sequence:

>894_bases
TTGATTTCTCCTGCTAAGATTAATCTTGGATTGGAAATTCCATTTAAACGTCTTGATGGGTTTCACGAAATTCGAAGTGT
ATTTTTAAAAATTTCTTGGGGTGATGATATAGAAATAGAACCTGCGTCTAACGGGGTTTTTGAACTTTTTTCCAATAACG
AAATTATATTAGAAAAACGTAAACTATATGATCAGGTTTCCGAAAGAGGTGATATTAAAAATAATATTCTTTATAAAACT
TTTATAAAGGCGAGATCTCTTTTTCCAGAACTTCCGGGTGTGAAAATTCATCTTACAAAACGGATTTCTCCGGCAGGTGG
TCTTGGAGGAGGAAGTACAAACGCGGCTTCTTTATTAAACTTTCTTTTTTCTTGGTGTCCTTTTTTTACTTCCGATGAAA
TGTTCGTTCTTGCTGCTGAAATTGGTTCCGATGTTCCTTTTTTTTTAGGAGAAGGACATGCCTTCGTTACTGGGAAGGGA
GAAATTTTAGAAGAAATAGAAGTTCATCATGGACAAGGAATTCTTGCGTTAACTCCGCAGGTGATGAATACATCGGAAAT
GTACTCCTTATTGAAAAAACCTTTACAAGAGAGCGCTTCTCAGAAAAATGGAAACACTCTGTCGAAAAATCTAATTTCTA
TACTAAAAAATGGAGATTGGAGCTCTCTTCAGGGTAGGCTCTGGAATGATTTTGAGCCGGTTGCCTTCCAACTTCATCCG
GAATTGGGAGTTCTCAAGGACAAATTTCTGGAGTTTGGATCCAGTTATTGTTCTTTGACCGGTTCGGGTTCGAGTATGTA
CGGGCTGGTGCAGGGTCTCGAGATCCAAGAAGAACTGTTACAAAGGCTTAGGCAGGAATTCTCAAATCTCACATTCGTAC
GATTTAACTTTTAG

Upstream 100 bases:

>100_bases
TGGAAGAATTGATAATACTTACAAATAAAATTCTAATAAAAGACGCGTGTGTCATTTGCCGGTTTGAATTTTTTTTTCAT
AAACTGTTTTGGAGTAAACG

Downstream 100 bases:

>100_bases
AAACTGGGCTGTCGCCAAGTGGTAAGGCAGCGGTTTTTGGTATCGCCATTTCCTAGGTTCGAATCCTAGCAGCCCAGCCA
ATGAAATTACTAACATAGAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MISPAKINLGLEIPFKRLDGFHEIRSVFLKISWGDDIEIEPASNGVFELFSNNEIILEKRKLYDQVSERGDIKNNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWCPFFTSDEMFVLAAEIGSDVPFFLGEGHAFVTGKG
EILEEIEVHHGQGILALTPQVMNTSEMYSLLKKPLQESASQKNGNTLSKNLISILKNGDWSSLQGRLWNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLQRLRQEFSNLTFVRFNF

Sequences:

>Translated_297_residues
MISPAKINLGLEIPFKRLDGFHEIRSVFLKISWGDDIEIEPASNGVFELFSNNEIILEKRKLYDQVSERGDIKNNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWCPFFTSDEMFVLAAEIGSDVPFFLGEGHAFVTGKG
EILEEIEVHHGQGILALTPQVMNTSEMYSLLKKPLQESASQKNGNTLSKNLISILKNGDWSSLQGRLWNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLQRLRQEFSNLTFVRFNF
>Mature_297_residues
MISPAKINLGLEIPFKRLDGFHEIRSVFLKISWGDDIEIEPASNGVFELFSNNEIILEKRKLYDQVSERGDIKNNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWCPFFTSDEMFVLAAEIGSDVPFFLGEGHAFVTGKG
EILEEIEVHHGQGILALTPQVMNTSEMYSLLKKPLQESASQKNGNTLSKNLISILKNGDWSSLQGRLWNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLQRLRQEFSNLTFVRFNF

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=274, Percent_Identity=29.1970802919708, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_LEPIC (Q72V75)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000412.1
- ProteinModelPortal:   Q72V75
- SMR:   Q72V75
- GeneID:   2770030
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10426
- NMPDR:   fig|267671.1.peg.412
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14613
- BioCyc:   LINT267671:LIC_10426-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33218; Mature: 33218

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: ACT_SITE 6-6 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPAKINLGLEIPFKRLDGFHEIRSVFLKISWGDDIEIEPASNGVFELFSNNEIILEKR
CCCCCEEEEEEECCHHHCCCHHHHHHHEEEEECCCCEEEEECCCCEEEEECCCEEEEHHH
KLYDQVSERGDIKNNILYKTFIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLN
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FLFSWCPFFTSDEMFVLAAEIGSDVPFFLGEGHAFVTGKGEILEEIEVHHGQGILALTPQ
HHHHHCCCCCCCCEEEEEEHHCCCCCEEEECCEEEEECCHHHHHHHHHHCCCEEEEECHH
VMNTSEMYSLLKKPLQESASQKNGNTLSKNLISILKNGDWSSLQGRLWNDFEPVAFQLHP
HCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLQRLRQEFSNLTFVRFNF
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MISPAKINLGLEIPFKRLDGFHEIRSVFLKISWGDDIEIEPASNGVFELFSNNEIILEKR
CCCCCEEEEEEECCHHHCCCHHHHHHHEEEEECCCCEEEEECCCCEEEEECCCEEEEHHH
KLYDQVSERGDIKNNILYKTFIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLN
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FLFSWCPFFTSDEMFVLAAEIGSDVPFFLGEGHAFVTGKGEILEEIEVHHGQGILALTPQ
HHHHHCCCCCCCCEEEEEEHHCCCCCEEEECCEEEEECCHHHHHHHHHHCCCEEEEECHH
VMNTSEMYSLLKKPLQESASQKNGNTLSKNLISILKNGDWSSLQGRLWNDFEPVAFQLHP
HCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLQRLRQEFSNLTFVRFNF
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA