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The map label for this gene is nifA [H]

Identifier: 45656042

GI number: 45656042

Start: 157135

End: 159234

Strand: Direct

Name: nifA [H]

Synonym: LIC10132

Alternate gene names: 45656042

Gene position: 157135-159234 (Clockwise)

Preceding gene: 45656039

Following gene: 45656044

Centisome position: 3.67

GC content: 37.05

Gene sequence:

>2100_bases
ATGTCAGGATATGTGAAGCCTAGCCCCTTAAGATCTATACAAGAAGTTGCTACGGCAATGAATTCTACCTTAGATCCGGA
TAAACTTCTGGATTTGATTTTAGAACGTTGCATTCAGATTTGCGAAGTAGGCTCCGGCTCTTTGATGTTGATTAATGAGA
AAGAAAACGTTTTAGATATAGTGACTTTTCGTGGGATGAATCCTTCCGTTCGAACTAAGGTAAAACTGAAAGTTGGAGAA
GGAATCACCGGAATTGTAGCCGCGTCGGGCGAAGGTATGATCGTAAGTGATGTTACCGCTAATCCACATTACATTTCCAT
AAAGGATGATATTATGTCGGAACTTGCGGTTCCGATGATTGTTGAAGACGTAGTGATCGGAGTAATTTCTTTGGACTCCA
GTCGTAAAGGAGCTTTCAACGAAGAACATTTAGAAATTATTTCTACTCTTGCTAATCAAGCTGCCCAGATTTTTAAGAAC
TTACAAATTTTTAGACAACTCGAACAAAAAAATAAAATTCAACAAGTGCTAATTGATATTTCCAGAACTGTTACTTCCAC
TTTGGTTCTACAGGAAATTTTTGAAGATATTATGGATCGTTTGGAAAAATCCCTAAATTTAGAACGCGGAAGTATTGTTC
TTTTCGAACCTGAAAAAGCGATTTTGAAATTGGAAGCAGCCTCCGGTTTAACTGCGGAAGAAATGGAAAAAGGGGTTTAT
CTTCCAGGAGAAGGTGTTACTGGAAAGGTTTTTGAAACAGGGGAACCAATTATCATCGAATCTATTGCAAATGACGAAAA
TTTTATAAACCGAGTTGGAAATGCGGCTCATTTTAAAAATAATCCGGAAAACGTTAGTTTTCTTGCGGCTCCTATCAAAT
CGGATACGGATGTCTTGGGAGTGGTAAGCGTTTATTTCGTTCATAAAAAATACATCGATTTAAAAACTTATTTAGATTTT
TTGCAAGTAGTTGCATCCGTAATTTATCAAGCGATTCGGATTCAAAAACTGATCGACGAGGAAAAAAGGGAGATTTCGAG
AGAGAACGTTCTTCTCAAAAGAGAATTAAAGAATAAATATAAATTTGGTTCTTTGATCGGTAAATCCAAACCGATGGAAA
AACTTTTCGAAATGATTCATCTTGTTTCCGATTCTAGAGCTTCTGTTTTGATTACTGGAGAATCTGGTACCGGTAAAGAA
ATGATTGCCTCTGCAATTCATTATAATTCTTCTCGCGCTGATAAGCCTTTTATCAAGATCAACTGCGCTGCAATTCCTGA
AAACTTACTTGAGAGCGAACTCTTTGGCCATAAGAAAGGTTCTTTTACGGGAGCTGTTTCGGATAAAAAAGGTAAGTTCG
AAATGGCAGATACCGGAACAATTTTCTTGGATGAAATCGGAGAGATGGATCTCAATTTACAATCTAAACTTTTGAGAGTT
CTGCAAGAGAAAGAGATTGAAGCCGTCGGTTCTGTTAAACCTAAAAAAATTGACGTAAGGGTAATAGCGGCGACTAACGC
GGATTTGGAAAAATTAATTTCTGAAAAAAGATTCAGGCCGGACCTTTTTTACAGACTAAACGTCGTAAATATGGTAACTC
CTCCTCTTCGTGAAAGATCGGATGACATTCCACTTTTGATCAATCACTTTATTGCAAAGTATGCCGAAGAAAACGGTAAA
AAAATTACGGGAGTCACGAGAGAGGCTCATAAACTTTTGATGAATTACAGTTGGCCTGGAAATGTTCGAGAACTTGAAAA
TGTAATAGAACGTGCGGTTGTACTTTCTCAACTAGAAATGTTGGATATTCAAGATTTTTCTGAGATCAATGGACGTCTTC
TTTACGGAGATGAGGATTTTGATCCGGAAACTGGGGATTCTGAATCTTCTGTAGAAATTGCCAATTCTAGATTCTCCTCC
TCAAATTTGGATGCGCTTGATGGAAGGGCTATCGAAGTTGTAGTTGGAGAAGTAGAAGCCCGTTTGATTAAATACGCGAT
GAAGAAGTTCAAATATACAAAAACAAGAGTTGCTAAATTTTTAGGAATCAATCGAAATACTTTGGATAAAAAAATCAAAG
ATCTCAAAATCGATTATTGA

Upstream 100 bases:

>100_bases
TAAATATTTATTAGCATTATTTAAAAAAATCGCTTTATAAATAAGCGAAAAATAAAATTTGAATTGACTTCAAAATTATC
AAATCGCAAGAATTTTAAGC

Downstream 100 bases:

>100_bases
TATTTAGGTCGTTTAGAGTTTGGGTTAATTTCGGGACTTGTAATTATTTTCGTTTACTACTTTCACAATTGACAAAACGT
TTTATCCAAAACCATAGTGG

Product: sigma 54 activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MSGYVKPSPLRSIQEVATAMNSTLDPDKLLDLILERCIQICEVGSGSLMLINEKENVLDIVTFRGMNPSVRTKVKLKVGE
GITGIVAASGEGMIVSDVTANPHYISIKDDIMSELAVPMIVEDVVIGVISLDSSRKGAFNEEHLEIISTLANQAAQIFKN
LQIFRQLEQKNKIQQVLIDISRTVTSTLVLQEIFEDIMDRLEKSLNLERGSIVLFEPEKAILKLEAASGLTAEEMEKGVY
LPGEGVTGKVFETGEPIIIESIANDENFINRVGNAAHFKNNPENVSFLAAPIKSDTDVLGVVSVYFVHKKYIDLKTYLDF
LQVVASVIYQAIRIQKLIDEEKREISRENVLLKRELKNKYKFGSLIGKSKPMEKLFEMIHLVSDSRASVLITGESGTGKE
MIASAIHYNSSRADKPFIKINCAAIPENLLESELFGHKKGSFTGAVSDKKGKFEMADTGTIFLDEIGEMDLNLQSKLLRV
LQEKEIEAVGSVKPKKIDVRVIAATNADLEKLISEKRFRPDLFYRLNVVNMVTPPLRERSDDIPLLINHFIAKYAEENGK
KITGVTREAHKLLMNYSWPGNVRELENVIERAVVLSQLEMLDIQDFSEINGRLLYGDEDFDPETGDSESSVEIANSRFSS
SNLDALDGRAIEVVVGEVEARLIKYAMKKFKYTKTRVAKFLGINRNTLDKKIKDLKIDY

Sequences:

>Translated_699_residues
MSGYVKPSPLRSIQEVATAMNSTLDPDKLLDLILERCIQICEVGSGSLMLINEKENVLDIVTFRGMNPSVRTKVKLKVGE
GITGIVAASGEGMIVSDVTANPHYISIKDDIMSELAVPMIVEDVVIGVISLDSSRKGAFNEEHLEIISTLANQAAQIFKN
LQIFRQLEQKNKIQQVLIDISRTVTSTLVLQEIFEDIMDRLEKSLNLERGSIVLFEPEKAILKLEAASGLTAEEMEKGVY
LPGEGVTGKVFETGEPIIIESIANDENFINRVGNAAHFKNNPENVSFLAAPIKSDTDVLGVVSVYFVHKKYIDLKTYLDF
LQVVASVIYQAIRIQKLIDEEKREISRENVLLKRELKNKYKFGSLIGKSKPMEKLFEMIHLVSDSRASVLITGESGTGKE
MIASAIHYNSSRADKPFIKINCAAIPENLLESELFGHKKGSFTGAVSDKKGKFEMADTGTIFLDEIGEMDLNLQSKLLRV
LQEKEIEAVGSVKPKKIDVRVIAATNADLEKLISEKRFRPDLFYRLNVVNMVTPPLRERSDDIPLLINHFIAKYAEENGK
KITGVTREAHKLLMNYSWPGNVRELENVIERAVVLSQLEMLDIQDFSEINGRLLYGDEDFDPETGDSESSVEIANSRFSS
SNLDALDGRAIEVVVGEVEARLIKYAMKKFKYTKTRVAKFLGINRNTLDKKIKDLKIDY
>Mature_698_residues
SGYVKPSPLRSIQEVATAMNSTLDPDKLLDLILERCIQICEVGSGSLMLINEKENVLDIVTFRGMNPSVRTKVKLKVGEG
ITGIVAASGEGMIVSDVTANPHYISIKDDIMSELAVPMIVEDVVIGVISLDSSRKGAFNEEHLEIISTLANQAAQIFKNL
QIFRQLEQKNKIQQVLIDISRTVTSTLVLQEIFEDIMDRLEKSLNLERGSIVLFEPEKAILKLEAASGLTAEEMEKGVYL
PGEGVTGKVFETGEPIIIESIANDENFINRVGNAAHFKNNPENVSFLAAPIKSDTDVLGVVSVYFVHKKYIDLKTYLDFL
QVVASVIYQAIRIQKLIDEEKREISRENVLLKRELKNKYKFGSLIGKSKPMEKLFEMIHLVSDSRASVLITGESGTGKEM
IASAIHYNSSRADKPFIKINCAAIPENLLESELFGHKKGSFTGAVSDKKGKFEMADTGTIFLDEIGEMDLNLQSKLLRVL
QEKEIEAVGSVKPKKIDVRVIAATNADLEKLISEKRFRPDLFYRLNVVNMVTPPLRERSDDIPLLINHFIAKYAEENGKK
ITGVTREAHKLLMNYSWPGNVRELENVIERAVVLSQLEMLDIQDFSEINGRLLYGDEDFDPETGDSESSVEIANSRFSSS
NLDALDGRAIEVVVGEVEARLIKYAMKKFKYTKTRVAKFLGINRNTLDKKIKDLKIDY

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=373, Percent_Identity=43.6997319034853, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI87082117, Length=446, Percent_Identity=40.1345291479821, Blast_Score=302, Evalue=6e-83,
Organism=Escherichia coli, GI1789087, Length=441, Percent_Identity=39.0022675736961, Blast_Score=282, Evalue=5e-77,
Organism=Escherichia coli, GI1790437, Length=332, Percent_Identity=45.4819277108434, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=41.5929203539823, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=41.6167664670659, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=53.2188841201717, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=53.6480686695279, Blast_Score=252, Evalue=6e-68,
Organism=Escherichia coli, GI1786524, Length=354, Percent_Identity=37.5706214689266, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=38.5074626865672, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787583, Length=355, Percent_Identity=34.6478873239437, Blast_Score=202, Evalue=8e-53,
Organism=Escherichia coli, GI1789828, Length=296, Percent_Identity=31.7567567567568, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI87081858, Length=343, Percent_Identity=29.7376093294461, Blast_Score=136, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78159; Mature: 78028

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYVKPSPLRSIQEVATAMNSTLDPDKLLDLILERCIQICEVGSGSLMLINEKENVLDI
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEE
VTFRGMNPSVRTKVKLKVGEGITGIVAASGEGMIVSDVTANPHYISIKDDIMSELAVPMI
EEECCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHH
VEDVVIGVISLDSSRKGAFNEEHLEIISTLANQAAQIFKNLQIFRQLEQKNKIQQVLIDI
HHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTVTSTLVLQEIFEDIMDRLEKSLNLERGSIVLFEPEKAILKLEAASGLTAEEMEKGVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHCCEE
LPGEGVTGKVFETGEPIIIESIANDENFINRVGNAAHFKNNPENVSFLAAPIKSDTDVLG
CCCCCCCCCEEECCCCEEEEECCCCHHHHHHCCCCHHCCCCCCCCEEEEECCCCCCHHHH
VVSVYFVHKKYIDLKTYLDFLQVVASVIYQAIRIQKLIDEEKREISRENVLLKRELKNKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFGSLIGKSKPMEKLFEMIHLVSDSRASVLITGESGTGKEMIASAIHYNSSRADKPFIKI
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEE
NCAAIPENLLESELFGHKKGSFTGAVSDKKGKFEMADTGTIFLDEIGEMDLNLQSKLLRV
EEHHCCHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHH
LQEKEIEAVGSVKPKKIDVRVIAATNADLEKLISEKRFRPDLFYRLNVVNMVTPPLRERS
HHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHCCC
DDIPLLINHFIAKYAEENGKKITGVTREAHKLLMNYSWPGNVRELENVIERAVVLSQLEM
CCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LDIQDFSEINGRLLYGDEDFDPETGDSESSVEIANSRFSSSNLDALDGRAIEVVVGEVEA
HCCCCHHHCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
RLIKYAMKKFKYTKTRVAKFLGINRNTLDKKIKDLKIDY
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGYVKPSPLRSIQEVATAMNSTLDPDKLLDLILERCIQICEVGSGSLMLINEKENVLDI
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEE
VTFRGMNPSVRTKVKLKVGEGITGIVAASGEGMIVSDVTANPHYISIKDDIMSELAVPMI
EEECCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHH
VEDVVIGVISLDSSRKGAFNEEHLEIISTLANQAAQIFKNLQIFRQLEQKNKIQQVLIDI
HHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTVTSTLVLQEIFEDIMDRLEKSLNLERGSIVLFEPEKAILKLEAASGLTAEEMEKGVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHCCEE
LPGEGVTGKVFETGEPIIIESIANDENFINRVGNAAHFKNNPENVSFLAAPIKSDTDVLG
CCCCCCCCCEEECCCCEEEEECCCCHHHHHHCCCCHHCCCCCCCCEEEEECCCCCCHHHH
VVSVYFVHKKYIDLKTYLDFLQVVASVIYQAIRIQKLIDEEKREISRENVLLKRELKNKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFGSLIGKSKPMEKLFEMIHLVSDSRASVLITGESGTGKEMIASAIHYNSSRADKPFIKI
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEE
NCAAIPENLLESELFGHKKGSFTGAVSDKKGKFEMADTGTIFLDEIGEMDLNLQSKLLRV
EEHHCCHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHH
LQEKEIEAVGSVKPKKIDVRVIAATNADLEKLISEKRFRPDLFYRLNVVNMVTPPLRERS
HHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHCCC
DDIPLLINHFIAKYAEENGKKITGVTREAHKLLMNYSWPGNVRELENVIERAVVLSQLEM
CCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LDIQDFSEINGRLLYGDEDFDPETGDSESSVEIANSRFSSSNLDALDGRAIEVVVGEVEA
HCCCCHHHCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
RLIKYAMKKFKYTKTRVAKFLGINRNTLDKKIKDLKIDY
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]