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The map label for this gene is ligT

Identifier: 45656008

GI number: 45656008

Start: 114286

End: 114834

Strand: Reverse

Name: ligT

Synonym: LIC10097

Alternate gene names: 45656008

Gene position: 114834-114286 (Counterclockwise)

Preceding gene: 45656009

Following gene: 45656007

Centisome position: 2.68

GC content: 34.97

Gene sequence:

>549_bases
ATGAGAACTTTTCTCGGAATCTCTATTCCAGAAGAAATAAAAGAACAATTAACTTCGATTTGTTACGGTCTTTCGGACAT
TCGATGGGTTCCAAAAGAAAACTTTCACATCACCTTAGTATTCTTAGGCGAACAATCCTCTGAATGTCTGGACATTCTAT
CCGACTTTTGCTCGGAAATATCTTTTACCGAATTTGATCTGAGTTTAAAATCAGTGGGAACTTTCGGGAAACAAAAATCT
CCTTCTATCCTTTTTGCAGCTGTAAAACCTTCCATCGAACTTTCACAACTTCACAAAACTTTAGATTCCGGAATTCGCAA
ACTTGGTTTTTCTATAGATCGACAGAATTATCAGCCTCATCTTACAATCGGCCGTTTTAAAAATTCGAACGAAACAAGAG
TAATTATGTACTTGGAAGAATTTGCAAATTTTGTTTCTTCCAATTTTAAGGTTTCCGAATTTCATATCTACTCTTCTAAA
ACATTCTCAAATGGACCGGTCTATTCCATCGAAGAATCTTTTTCACTTTTACCGTCTTACAAAATATGA

Upstream 100 bases:

>100_bases
CTCTTAGAGGAATTTTAAGAGTAAAAGAAGTTTATAATTTCATAAAAGAATAACGTAGGAATCTAGTTGCGTAAACCCAA
AAGGATTCTAAACTTTAAGT

Downstream 100 bases:

>100_bases
AAATCCTAAATTATAAAGTTTTAGAAAATTCTAAAATAGGAAACAAACCTTGAAAGACTTCTCCGATTTTTTTCGAAACC
CTCACATTTGGGATCGAGAA

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MRTFLGISIPEEIKEQLTSICYGLSDIRWVPKENFHITLVFLGEQSSECLDILSDFCSEISFTEFDLSLKSVGTFGKQKS
PSILFAAVKPSIELSQLHKTLDSGIRKLGFSIDRQNYQPHLTIGRFKNSNETRVIMYLEEFANFVSSNFKVSEFHIYSSK
TFSNGPVYSIEESFSLLPSYKI

Sequences:

>Translated_182_residues
MRTFLGISIPEEIKEQLTSICYGLSDIRWVPKENFHITLVFLGEQSSECLDILSDFCSEISFTEFDLSLKSVGTFGKQKS
PSILFAAVKPSIELSQLHKTLDSGIRKLGFSIDRQNYQPHLTIGRFKNSNETRVIMYLEEFANFVSSNFKVSEFHIYSSK
TFSNGPVYSIEESFSLLPSYKI
>Mature_182_residues
MRTFLGISIPEEIKEQLTSICYGLSDIRWVPKENFHITLVFLGEQSSECLDILSDFCSEISFTEFDLSLKSVGTFGKQKS
PSILFAAVKPSIELSQLHKTLDSGIRKLGFSIDRQNYQPHLTIGRFKNSNETRVIMYLEEFANFVSSNFKVSEFHIYSSK
TFSNGPVYSIEESFSLLPSYKI

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20750; Mature: 20750

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFLGISIPEEIKEQLTSICYGLSDIRWVPKENFHITLVFLGEQSSECLDILSDFCSEI
CCCEECCCCCHHHHHHHHHHHHCHHHCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
SFTEFDLSLKSVGTFGKQKSPSILFAAVKPSIELSQLHKTLDSGIRKLGFSIDRQNYQPH
CCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEECCCCCCCE
LTIGRFKNSNETRVIMYLEEFANFVSSNFKVSEFHIYSSKTFSNGPVYSIEESFSLLPSY
EEEEEECCCCCCEEEEEHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCHHHCCCCC
KI
CC
>Mature Secondary Structure
MRTFLGISIPEEIKEQLTSICYGLSDIRWVPKENFHITLVFLGEQSSECLDILSDFCSEI
CCCEECCCCCHHHHHHHHHHHHCHHHCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
SFTEFDLSLKSVGTFGKQKSPSILFAAVKPSIELSQLHKTLDSGIRKLGFSIDRQNYQPH
CCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEECCCCCCCE
LTIGRFKNSNETRVIMYLEEFANFVSSNFKVSEFHIYSSKTFSNGPVYSIEESFSLLPSY
EEEEEECCCCCCEEEEEHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCHHHCCCCC
KI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]