Click here to switch to the map view.

The map label for this gene is ygcA [C]

Identifier: 45655997

GI number: 45655997

Start: 100706

End: 101953

Strand: Direct

Name: ygcA [C]

Synonym: LIC10086

Alternate gene names: 45655997

Gene position: 100706-101953 (Clockwise)

Preceding gene: 45655991

Following gene: 45655998

Centisome position: 2.35

GC content: 32.85

Gene sequence:

>1248_bases
ATGAAAAGTGATTCTGAGGGACATAAAAGTAGTTCTAAAATACAAATTCATAAAGGTAAAATTTTACTTAAACCTCGTTC
TTGGGTGAATTTAGGTTATTCGATCGCAAATTCAGAAAAAGAAACTTTCTTCTTAAAAAATGCGATTCCGGGTGAAACGG
TTGATACGGTTCTTCTTAAAAGATCTGGTTCTTTATTTTGGGGAGTTGCTTCCGAAATTCAAGAGGTTTCATCGGAGAGA
ATTTCTTCCGATTGTAGCATATTTCCTCGTTGTGGTGGTTGTTCTTATCGTCACGTTTCTTATCAAAAAGAATTAGAGAT
CAAGAAGTTTCTGCTTCAAGAAACGTTGGAACATTTTTTATCCAAAAAACATATTCAAATTCCTGAAATTGAAATTCTCA
GCGGTGATCCGAACGGTTATAGAAACACGGCTCAGATCCAATTAGGATTTGCTGGGAATCAAAGACTCGCAGGTTTTTAC
GAGGAATTTTCTCATTCTATTGTAAATCTTCCCGAAGAAGGGTGTAAAAATTTACCTCAAGAAATGAATTTTGCTTTTGC
TGAGTTTTTAAAACAGGAAGTTAAGGGGTCAAAACAGATTTTAAAGTCAAAAACACTTTCCTTTCGATTAGAAGGGACAA
AAGTTATTTCCTACAAAAAAAAATCGGTAAGTTTTAGTGAAAATATTAGAATTCCAGAATTAAAGAAGATCGTTTGGGAA
ATTCCGGCAGGAGGATTCTCCCAAGTAAATCGTTATCTCATTGCTCCTTGGTTGGAAAAAATTTTTGAATTAGTTCCAAA
TAATCAAAATAGAATATTAGAATTATATTGTGGATCAGGTTTGATCGCAATTGCTCTTAAGTCTAAGACTACAAGTTGGT
TGGGTTATGAGATTTCTTCGGATTGTGTTCAACAAGCAAAGAGAAATGTTTTACTAAATGGTATTTCATCTTGTGATTTT
AAAACGTTGAATCTGGAAACAGATTGGATTGATTCCGAAGAAGTTTTGAATTCTTCTTTTTGGATTATGAATCCTCCTAG
AGCGGGACTCTCTAAAAAAGTTTTACAAACTCTAATAAAAACAAGTCCAAATGGTTTCTTATATTCTAGTTGCAATCATT
CTACCCTTGTAAGAGATCTTTCTCTGTTTTTAAATAAAGATTATAAACTTTCTAATGTAACGTTAGTCGACTTTTTTCCA
AGAACGAAACACTTTGAAGTTATCGTAAAAGTCGAAAAGAAAGATTAA

Upstream 100 bases:

>100_bases
ACGACGGAATTACTTTACCGTGAGAAAACCCCTCTGTGTCAAGGGAATTGTTCTTTGCGTTCTTGGATCTTTGTATTTCC
TGGAATCACTATAGATAGAA

Downstream 100 bases:

>100_bases
AATTTTATTATTACTCAGAGTTTCTATATTTAGAGTTTTTGAAAAATTAATTCTCCATTTGTTTTATGGAAACGGTTTAT
TGAAGCAGTTTTGTTAATCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKSDSEGHKSSSKIQIHKGKILLKPRSWVNLGYSIANSEKETFFLKNAIPGETVDTVLLKRSGSLFWGVASEIQEVSSER
ISSDCSIFPRCGGCSYRHVSYQKELEIKKFLLQETLEHFLSKKHIQIPEIEILSGDPNGYRNTAQIQLGFAGNQRLAGFY
EEFSHSIVNLPEEGCKNLPQEMNFAFAEFLKQEVKGSKQILKSKTLSFRLEGTKVISYKKKSVSFSENIRIPELKKIVWE
IPAGGFSQVNRYLIAPWLEKIFELVPNNQNRILELYCGSGLIAIALKSKTTSWLGYEISSDCVQQAKRNVLLNGISSCDF
KTLNLETDWIDSEEVLNSSFWIMNPPRAGLSKKVLQTLIKTSPNGFLYSSCNHSTLVRDLSLFLNKDYKLSNVTLVDFFP
RTKHFEVIVKVEKKD

Sequences:

>Translated_415_residues
MKSDSEGHKSSSKIQIHKGKILLKPRSWVNLGYSIANSEKETFFLKNAIPGETVDTVLLKRSGSLFWGVASEIQEVSSER
ISSDCSIFPRCGGCSYRHVSYQKELEIKKFLLQETLEHFLSKKHIQIPEIEILSGDPNGYRNTAQIQLGFAGNQRLAGFY
EEFSHSIVNLPEEGCKNLPQEMNFAFAEFLKQEVKGSKQILKSKTLSFRLEGTKVISYKKKSVSFSENIRIPELKKIVWE
IPAGGFSQVNRYLIAPWLEKIFELVPNNQNRILELYCGSGLIAIALKSKTTSWLGYEISSDCVQQAKRNVLLNGISSCDF
KTLNLETDWIDSEEVLNSSFWIMNPPRAGLSKKVLQTLIKTSPNGFLYSSCNHSTLVRDLSLFLNKDYKLSNVTLVDFFP
RTKHFEVIVKVEKKD
>Mature_415_residues
MKSDSEGHKSSSKIQIHKGKILLKPRSWVNLGYSIANSEKETFFLKNAIPGETVDTVLLKRSGSLFWGVASEIQEVSSER
ISSDCSIFPRCGGCSYRHVSYQKELEIKKFLLQETLEHFLSKKHIQIPEIEILSGDPNGYRNTAQIQLGFAGNQRLAGFY
EEFSHSIVNLPEEGCKNLPQEMNFAFAEFLKQEVKGSKQILKSKTLSFRLEGTKVISYKKKSVSFSENIRIPELKKIVWE
IPAGGFSQVNRYLIAPWLEKIFELVPNNQNRILELYCGSGLIAIALKSKTTSWLGYEISSDCVQQAKRNVLLNGISSCDF
KTLNLETDWIDSEEVLNSSFWIMNPPRAGLSKKVLQTLIKTSPNGFLYSSCNHSTLVRDLSLFLNKDYKLSNVTLVDFFP
RTKHFEVIVKVEKKD

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Homo sapiens, GI269784680, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI269784676, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI20149688, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI269784678, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789148, Length=417, Percent_Identity=25.1798561151079, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1787083, Length=144, Percent_Identity=28.4722222222222, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790403, Length=241, Percent_Identity=25.3112033195021, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24666579, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y086_LEPIC (Q72W54)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000083.1
- ProteinModelPortal:   Q72W54
- SMR:   Q72W54
- GeneID:   2772235
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10086
- NMPDR:   fig|267671.1.peg.83
- HOGENOM:   HBG530261
- OMA:   APYRAFK
- ProtClustDB:   CLSK573344
- BioCyc:   LINT267671:LIC_10086-MONOMER
- InterPro:   IPR010280

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: NA

Molecular weight: Translated: 47129; Mature: 47129

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 371-371 BINDING 248-248 BINDING 276-276 BINDING 297-297 BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDSEGHKSSSKIQIHKGKILLKPRSWVNLGYSIANSEKETFFLKNAIPGETVDTVLLK
CCCCCCCCCCCCEEEEECCEEEECCCHHHHCCEEECCCCCCEEEEECCCCCCHHHHHHHH
RSGSLFWGVASEIQEVSSERISSDCSIFPRCGGCSYRHVSYQKELEIKKFLLQETLEHFL
CCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
SKKHIQIPEIEILSGDPNGYRNTAQIQLGFAGNQRLAGFYEEFSHSIVNLPEEGCKNLPQ
CCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCH
EMNFAFAEFLKQEVKGSKQILKSKTLSFRLEGTKVISYKKKSVSFSENIRIPELKKIVWE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCHHCCCCCHHHHHHHHH
IPAGGFSQVNRYLIAPWLEKIFELVPNNQNRILELYCGSGLIAIALKSKTTSWLGYEISS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEECEEHHH
DCVQQAKRNVLLNGISSCDFKTLNLETDWIDSEEVLNSSFWIMNPPRAGLSKKVLQTLIK
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHH
TSPNGFLYSSCNHSTLVRDLSLFLNKDYKLSNVTLVDFFPRTKHFEVIVKVEKKD
CCCCCEEEECCCCHHHHHHHHHHHCCCCEECCEEEEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKSDSEGHKSSSKIQIHKGKILLKPRSWVNLGYSIANSEKETFFLKNAIPGETVDTVLLK
CCCCCCCCCCCCEEEEECCEEEECCCHHHHCCEEECCCCCCEEEEECCCCCCHHHHHHHH
RSGSLFWGVASEIQEVSSERISSDCSIFPRCGGCSYRHVSYQKELEIKKFLLQETLEHFL
CCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
SKKHIQIPEIEILSGDPNGYRNTAQIQLGFAGNQRLAGFYEEFSHSIVNLPEEGCKNLPQ
CCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCH
EMNFAFAEFLKQEVKGSKQILKSKTLSFRLEGTKVISYKKKSVSFSENIRIPELKKIVWE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCHHCCCCCHHHHHHHHH
IPAGGFSQVNRYLIAPWLEKIFELVPNNQNRILELYCGSGLIAIALKSKTTSWLGYEISS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEECEEHHH
DCVQQAKRNVLLNGISSCDFKTLNLETDWIDSEEVLNSSFWIMNPPRAGLSKKVLQTLIK
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHH
TSPNGFLYSSCNHSTLVRDLSLFLNKDYKLSNVTLVDFFPRTKHFEVIVKVEKKD
CCCCCEEEECCCCHHHHHHHHHHHCCCCEECCEEEEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA