Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 45655988

GI number: 45655988

Start: 92703

End: 93548

Strand: Direct

Name: dapF

Synonym: LIC10074

Alternate gene names: 45655988

Gene position: 92703-93548 (Clockwise)

Preceding gene: 45655986

Following gene: 45655991

Centisome position: 2.17

GC content: 39.95

Gene sequence:

>846_bases
GTGGCTTCGCTTAAGTTCACCAAAATGGAAGGAATCGGTAACGACTACGTTTATATCGATTCTACTCGGAATGATATTCG
TCTAACACCAGAACAGATTCAAAAAATATCCGATCGTAATTTCGGAATTGGAAGCGACGGAGTCATTTTTATTCGAAATT
CAAAACAAGGCGATTTTATGATGGATATGTACAATTCGGATGGAAGTTCTTCCGAAATGTGCGGGAACGGAATTCGTTGT
GTTGCCAAATACATATATGATCACGGTTTAACGAGTTCTAAAAATCCTAAGATCGAAACTGGCGCGGGAATTTTGGAAGT
AGATTTAAAAATTGGATCCGGTAACAAGGTTGATCTTGTAAGCGTAGATATGGGAAAGCCGGTTCTAGTTCCATCTCAAA
TTCCGGTCGTTTGGAAAAATGAAGAAACAATCATTGATCAACCACTTGAGATCGGAGATAAAAATTTAAAATTTACTGCG
GTTAGTATGGGAAATCCACACTGTGTAATCTTTGTGGATGATAGCGATGAATTTCCAGTAAGAGGAATTGGACCGTTAAT
CGAACGTCATTCTATCTTTCCGAAAAGAGTGAATGTGGAATTTGTTACTATTCGTGGAAAAGACCATCTTTATCAAAGAA
CTTGGGAAAGAGGGGCGGGAGAAACTCTCGCTTGTGGGACAGGAGCTTGTGCCGTGATGGTGGCTGGGAATTTAACGGGA
AGATCTGGAAAAGACGTTCAAATCGATTTGAGAGGTGGCACTCTTAGAATTCAGTGGCAAGAATCTGGAAACATTTTAAT
GACTGGCCCAGCCCGTGAGATATTTTCAGGAGAAATAGAAATTTAG

Upstream 100 bases:

>100_bases
CGGAAAAGCCAGAAAAAACTATAAAACGTTTTCTCGTTTTTGAATTTCCTCGGATCACAGTCTAAGAAAATATAGAAATC
AATTTATTTTAGGAGTTTTT

Downstream 100 bases:

>100_bases
TATATTCTAAACTACTTCCGTCTTTTGTAAGTTTTCCATTCTCACTCGAACTTCGTTGGAAAGTTTTTTGAGATTGTTTT
CTAGTTTTCCTTCTAGATAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MASLKFTKMEGIGNDYVYIDSTRNDIRLTPEQIQKISDRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRC
VAKYIYDHGLTSSKNPKIETGAGILEVDLKIGSGNKVDLVSVDMGKPVLVPSQIPVVWKNEETIIDQPLEIGDKNLKFTA
VSMGNPHCVIFVDDSDEFPVRGIGPLIERHSIFPKRVNVEFVTIRGKDHLYQRTWERGAGETLACGTGACAVMVAGNLTG
RSGKDVQIDLRGGTLRIQWQESGNILMTGPAREIFSGEIEI

Sequences:

>Translated_281_residues
MASLKFTKMEGIGNDYVYIDSTRNDIRLTPEQIQKISDRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRC
VAKYIYDHGLTSSKNPKIETGAGILEVDLKIGSGNKVDLVSVDMGKPVLVPSQIPVVWKNEETIIDQPLEIGDKNLKFTA
VSMGNPHCVIFVDDSDEFPVRGIGPLIERHSIFPKRVNVEFVTIRGKDHLYQRTWERGAGETLACGTGACAVMVAGNLTG
RSGKDVQIDLRGGTLRIQWQESGNILMTGPAREIFSGEIEI
>Mature_280_residues
ASLKFTKMEGIGNDYVYIDSTRNDIRLTPEQIQKISDRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRCV
AKYIYDHGLTSSKNPKIETGAGILEVDLKIGSGNKVDLVSVDMGKPVLVPSQIPVVWKNEETIIDQPLEIGDKNLKFTAV
SMGNPHCVIFVDDSDEFPVRGIGPLIERHSIFPKRVNVEFVTIRGKDHLYQRTWERGAGETLACGTGACAVMVAGNLTGR
SGKDVQIDLRGGTLRIQWQESGNILMTGPAREIFSGEIEI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=37.5886524822695, Blast_Score=211, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_LEPIC (Q72W63)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000074.1
- ProteinModelPortal:   Q72W63
- SMR:   Q72W63
- GeneID:   2769936
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10074
- NMPDR:   fig|267671.1.peg.74
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   LINT267671:LIC_10074-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30868; Mature: 30736

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLKFTKMEGIGNDYVYIDSTRNDIRLTPEQIQKISDRNFGIGSDGVIFIRNSKQGDFM
CCCCEEEEECCCCCCEEEEECCCCCEEECHHHHHHHCCCCCCCCCCCEEEEECCCCCCEE
MDMYNSDGSSSEMCGNGIRCVAKYIYDHGLTSSKNPKIETGAGILEVDLKIGSGNKVDLV
EEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCEEEEE
SVDMGKPVLVPSQIPVVWKNEETIIDQPLEIGDKNLKFTAVSMGNPHCVIFVDDSDEFPV
EECCCCCEEECCCCCEEECCCCEEECCCHHCCCCCEEEEEEECCCCEEEEEEECCCCCCC
RGIGPLIERHSIFPKRVNVEFVTIRGKDHLYQRTWERGAGETLACGTGACAVMVAGNLTG
CCCCHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEEECCCCC
RSGKDVQIDLRGGTLRIQWQESGNILMTGPAREIFSGEIEI
CCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHCCCEECC
>Mature Secondary Structure 
ASLKFTKMEGIGNDYVYIDSTRNDIRLTPEQIQKISDRNFGIGSDGVIFIRNSKQGDFM
CCCEEEEECCCCCCEEEEECCCCCEEECHHHHHHHCCCCCCCCCCCEEEEECCCCCCEE
MDMYNSDGSSSEMCGNGIRCVAKYIYDHGLTSSKNPKIETGAGILEVDLKIGSGNKVDLV
EEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCEEEEE
SVDMGKPVLVPSQIPVVWKNEETIIDQPLEIGDKNLKFTAVSMGNPHCVIFVDDSDEFPV
EECCCCCEEECCCCCEEECCCCEEECCCHHCCCCCEEEEEEECCCCEEEEEEECCCCCCC
RGIGPLIERHSIFPKRVNVEFVTIRGKDHLYQRTWERGAGETLACGTGACAVMVAGNLTG
CCCCHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEEECCCCC
RSGKDVQIDLRGGTLRIQWQESGNILMTGPAREIFSGEIEI
CCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA