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The map label for this gene is dusB [H]

Identifier: 45655921

GI number: 45655921

Start: 11133

End: 12122

Strand: Direct

Name: dusB [H]

Synonym: LIC10007

Alternate gene names: 45655921

Gene position: 11133-12122 (Clockwise)

Preceding gene: 45655920

Following gene: 45655922

Centisome position: 0.26

GC content: 37.58

Gene sequence:

>990_bases
ATGATCCGGATCGGAAACGTAACGATTCCGGGGAAAATTTCTCTTTCTCCGATGGCGGGAATTTCAGATTCACCTACTCG
TAGAATTTGTAGAAAATTTGGAGCTGCTTTTTCTTATACCGAGTTTGTAAATACAGACGAACTCGTTCATCGAGCGCCTA
AGGCTCTGAAAATGTTTCGCTACAAATCGGAAGAACGTCCGATCACATTTCAAATTTTTGGAAACAGATTGGAAATTATC
GCAGAAGCTGCAGAGATCATTCAGGAATTAAAACCCGACATCATCGATTTAAACATGGGCTGTTCCACTCGTAAGGTTTC
CTTACGAGGAGCAGGCGCTGGACTTTTGCGTAGACCTTTGTTAGCTGGCAAAATTATAGAAGCTATAAAAAAACGAGTGA
ACATTCCCGTTACAGCAAAGATACGGATTGGATGGGATTCAACTTCTCGAAACTATCTTGAAGTTTCTAAAATCTTGGAA
GAATCCGGTGTAGATGCTCTTACGGTTCACGGTCGTACAAAAGAAATGGCTTATACAGGATTTGCGGATTGGGATGCAAT
CGGAGAAATAAAAGTAAATCGGAAAATTCCAATTTTTGGCAACGGAGATATTAAAAGTTTTCAGGAGGCGAATTTTAAGA
TCGATAAATATTTGTTAGACGGAGTTTTGATCGGTAGAAACGCGATTGGAAATCCTTGGATTTTTTCCGGAGTTAAAAAA
GAAGAATTGTCTTGGTCTGAAGTTTTAGATGTAATTTTACAACATTTACGTTGGATGATTGAGGATTATGGAGAAGAATT
CGGTTTGGTTTTATTTAGAAAACATCTCGTAAAATATTTAAATGGCTTTGAATTTGATCTCGCTTGGAAAACGGAATTAT
TGGAAATTAAAGAGTGGAATCGTTTTGAAGATATTTTGCTCTCGGATAAAAAAAGAATCGAATTCACTGTGCCCACTTTT
GGAACTACTAAACGTTTTGCTTTTCCATAA

Upstream 100 bases:

>100_bases
ATTTCTAAATTAGGTAGAACTGCTGTAGGTGTTCGTATTGTGGATTTAAAAGACAACGATAAGGTTCAGGATTTTACAGT
TCTAGGAGAAAATTGATTTT

Downstream 100 bases:

>100_bases
CCATCCATGACAAACGACCATCACGATCTATCGCTTGAAGATTACTTGCAACGTGTGAGGGACCGTTTGCTTATTTGCCT
GGATAATTTTCTAGAAAAAA

Product: nifR3-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIRIGNVTIPGKISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFRYKSEERPITFQIFGNRLEII
AEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILE
ESGVDALTVHGRTKEMAYTGFADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKK
EELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGFEFDLAWKTELLEIKEWNRFEDILLSDKKRIEFTVPTF
GTTKRFAFP

Sequences:

>Translated_329_residues
MIRIGNVTIPGKISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFRYKSEERPITFQIFGNRLEII
AEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILE
ESGVDALTVHGRTKEMAYTGFADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKK
EELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGFEFDLAWKTELLEIKEWNRFEDILLSDKKRIEFTVPTF
GTTKRFAFP
>Mature_329_residues
MIRIGNVTIPGKISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFRYKSEERPITFQIFGNRLEII
AEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILE
ESGVDALTVHGRTKEMAYTGFADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKK
EELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGFEFDLAWKTELLEIKEWNRFEDILLSDKKRIEFTVPTF
GTTKRFAFP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=286, Percent_Identity=29.020979020979, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI239788462, Length=285, Percent_Identity=30.8771929824561, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI31742496, Length=262, Percent_Identity=29.7709923664122, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI40807366, Length=251, Percent_Identity=31.0756972111554, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI8923374, Length=277, Percent_Identity=27.4368231046931, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789660, Length=288, Percent_Identity=34.375, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=32.1428571428571, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693211, Length=297, Percent_Identity=27.9461279461279, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=285, Percent_Identity=27.0175438596491, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=236, Percent_Identity=26.6949152542373, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=242, Percent_Identity=29.3388429752066, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=311, Percent_Identity=25.7234726688103, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=288, Percent_Identity=26.3888888888889, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=303, Percent_Identity=26.0726072607261, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=30.1075268817204, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24580595, Length=228, Percent_Identity=32.0175438596491, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920448, Length=228, Percent_Identity=32.0175438596491, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=24.8888888888889, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37549; Mature: 37549

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGNVTIPGKISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFR
CEEECCEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
YKSEERPITFQIFGNRLEIIAEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPL
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHH
LAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILEESGVDALTVHGRTKEMAYTG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCHHEEECC
FADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKK
CCCCCCCCEEEECCEEEEEECCCCCCHHHCCCCHHHHHHCCEEECCCCCCCCCEECCCCH
EELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGFEFDLAWKTELLEIKEWN
HCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCC
RFEDILLSDKKRIEFTVPTFGTTKRFAFP
HHHHHHHCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIRIGNVTIPGKISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHRAPKALKMFR
CEEECCEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
YKSEERPITFQIFGNRLEIIAEAAEIIQELKPDIIDLNMGCSTRKVSLRGAGAGLLRRPL
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHH
LAGKIIEAIKKRVNIPVTAKIRIGWDSTSRNYLEVSKILEESGVDALTVHGRTKEMAYTG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCHHEEECC
FADWDAIGEIKVNRKIPIFGNGDIKSFQEANFKIDKYLLDGVLIGRNAIGNPWIFSGVKK
CCCCCCCCEEEECCEEEEEECCCCCCHHHCCCCHHHHHHCCEEECCCCCCCCCEECCCCH
EELSWSEVLDVILQHLRWMIEDYGEEFGLVLFRKHLVKYLNGFEFDLAWKTELLEIKEWN
HCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCC
RFEDILLSDKKRIEFTVPTFGTTKRFAFP
HHHHHHHCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]