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The map label for this gene is truB

Identifier: 161621773

GI number: 161621773

Start: 3288824

End: 3289753

Strand: Reverse

Name: truB

Synonym: LIC12703

Alternate gene names: 161621773

Gene position: 3289753-3288824 (Counterclockwise)

Preceding gene: 45658539

Following gene: 45658537

Centisome position: 76.91

GC content: 37.63

Gene sequence:

>930_bases
ATGAATCGCTCAGACTTACTAGAAAACCCACGAACACAGACTGAATCTGGATTTTTACTGATCCATAAACCGGTAGGTAT
GACTTCTTCGGATTTAGTGGTTACCGTTCGTAAAAAACTAGGATTTAAGAAAGTAGGTCATACTGGAACCTTGGACCGAG
CCGCAAGTGGCTTAATGATTCTTCCTATTGGAAGTTGTACTAGTTTTTCCAGCGTATTTTTAGAAAAAGAAAAATCCTAT
GAAGCATGGGTAAGGCCAGGAGAATCTACTGATTCCGGAGATAAAGAAGGTGAGATTTTAGAATCTCTTTCCAAAGAACA
AACTGAAACTTGGTTCCAAGAGCACCAAGAAAAATTAAGGGATTTATTTGAACAAGTGCCTACTTGGGAAACCCAAGAAG
CTCCTGAAGTTTCTGCTCTCAAGGTAAACGGTAGGCGAAGATCGGATCTATTTCGAGAAGGTGTAGCTCTTGTTCCAGCG
GTTCGAAAGATCAAAATATATCGGTATGAACTTGGAGAATTTTCTCCCGAATCTATTTCTTTTCAAATTCGAGTTTCTGC
AGGAACTTATATTCGTAAAATTGTAATGGATATTTCCGATCGAATCGGTTTTCCACTTCGTTTGGAGAAATTGGTTCGTA
CAAGTATTGGTAAGTTAAACTTAAATCAGGCCGATTCATACGAAAGTCTATTAGATGGAAAGATTAAAATTCATCCTCCA
GAAACGATTTTGGATTTTCCGACGGTAGAAGTTCCAGACACGGAAGTTAGGAATGTACTGAATGGTAGAAAAATTAAGTT
GGAATGGATTCCAGTGAATGAGTTTCTTTTGGTTTCTCCCGAAGAGGAGATTCTTGCTTGGTGTAAAAAAGAAGAACATG
GAATCCATGAATTAGATTATAAATATTTAAGGGTCTTTCCTAAAAATTAG

Upstream 100 bases:

>100_bases
GCCTCAGGTTTATTCCAGGCGACGTTGTCCGGAAAGTTAGGACTTAGAATTACACCTAAAATGCATTTTCTCTGGGATGA
GGAATATATTCAGAGTTTGG

Downstream 100 bases:

>100_bases
CTTTAAAGTGACGCTATTGCCTGGAAAATGGTAAAAAGGTATATTCACTCTATGATAGCTACTGAACAGAAAAAACAAAT
TATCAGTAATTTTGCCCGCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNRSDLLENPRTQTESGFLLIHKPVGMTSSDLVVTVRKKLGFKKVGHTGTLDRAASGLMILPIGSCTSFSSVFLEKEKSY
EAWVRPGESTDSGDKEGEILESLSKEQTETWFQEHQEKLRDLFEQVPTWETQEAPEVSALKVNGRRRSDLFREGVALVPA
VRKIKIYRYELGEFSPESISFQIRVSAGTYIRKIVMDISDRIGFPLRLEKLVRTSIGKLNLNQADSYESLLDGKIKIHPP
ETILDFPTVEVPDTEVRNVLNGRKIKLEWIPVNEFLLVSPEEEILAWCKKEEHGIHELDYKYLRVFPKN

Sequences:

>Translated_309_residues
MNRSDLLENPRTQTESGFLLIHKPVGMTSSDLVVTVRKKLGFKKVGHTGTLDRAASGLMILPIGSCTSFSSVFLEKEKSY
EAWVRPGESTDSGDKEGEILESLSKEQTETWFQEHQEKLRDLFEQVPTWETQEAPEVSALKVNGRRRSDLFREGVALVPA
VRKIKIYRYELGEFSPESISFQIRVSAGTYIRKIVMDISDRIGFPLRLEKLVRTSIGKLNLNQADSYESLLDGKIKIHPP
ETILDFPTVEVPDTEVRNVLNGRKIKLEWIPVNEFLLVSPEEEILAWCKKEEHGIHELDYKYLRVFPKN
>Mature_309_residues
MNRSDLLENPRTQTESGFLLIHKPVGMTSSDLVVTVRKKLGFKKVGHTGTLDRAASGLMILPIGSCTSFSSVFLEKEKSY
EAWVRPGESTDSGDKEGEILESLSKEQTETWFQEHQEKLRDLFEQVPTWETQEAPEVSALKVNGRRRSDLFREGVALVPA
VRKIKIYRYELGEFSPESISFQIRVSAGTYIRKIVMDISDRIGFPLRLEKLVRTSIGKLNLNQADSYESLLDGKIKIHPP
ETILDFPTVEVPDTEVRNVLNGRKIKLEWIPVNEFLLVSPEEEILAWCKKEEHGIHELDYKYLRVFPKN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=29.7777777777778, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI2367200, Length=289, Percent_Identity=26.643598615917, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_LEPIC (Q72NX5)

Other databases:

- EMBL:   AE016823
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12703
- NMPDR:   fig|267671.1.peg.2624
- HOGENOM:   HBG529271
- OMA:   EYLGTIR
- ProtClustDB:   CLSK2516725
- BioCyc:   LINT267671:LIC_12703-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 35334; Mature: 35334

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSDLLENPRTQTESGFLLIHKPVGMTSSDLVVTVRKKLGFKKVGHTGTLDRAASGLMI
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEHHHCCCCCCCCCCCHHHHCCCEEE
LPIGSCTSFSSVFLEKEKSYEAWVRPGESTDSGDKEGEILESLSKEQTETWFQEHQEKLR
EECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLFEQVPTWETQEAPEVSALKVNGRRRSDLFREGVALVPAVRKIKIYRYELGEFSPESIS
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHEEEEEEECCCCCCCCEE
FQIRVSAGTYIRKIVMDISDRIGFPLRLEKLVRTSIGKLNLNQADSYESLLDGKIKIHPP
EEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCC
ETILDFPTVEVPDTEVRNVLNGRKIKLEWIPVNEFLLVSPEEEILAWCKKEEHGIHELDY
CHHHCCCCCCCCCHHHHHHHCCCEEEEEEEECCCEEEECCHHHHHHHHHHHCCCCHHHCC
KYLRVFPKN
EEEEEECCC
>Mature Secondary Structure
MNRSDLLENPRTQTESGFLLIHKPVGMTSSDLVVTVRKKLGFKKVGHTGTLDRAASGLMI
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEHHHCCCCCCCCCCCHHHHCCCEEE
LPIGSCTSFSSVFLEKEKSYEAWVRPGESTDSGDKEGEILESLSKEQTETWFQEHQEKLR
EECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLFEQVPTWETQEAPEVSALKVNGRRRSDLFREGVALVPAVRKIKIYRYELGEFSPESIS
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHEEEEEEECCCCCCCCEE
FQIRVSAGTYIRKIVMDISDRIGFPLRLEKLVRTSIGKLNLNQADSYESLLDGKIKIHPP
EEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCC
ETILDFPTVEVPDTEVRNVLNGRKIKLEWIPVNEFLLVSPEEEILAWCKKEEHGIHELDY
CHHHCCCCCCCCCHHHHHHHCCCEEEEEEEECCCEEEECCHHHHHHHHHHHCCCCHHHCC
KYLRVFPKN
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA