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The map label for this gene is thiD [C]

Identifier: 45359202

GI number: 45359202

Start: 1580669

End: 1581871

Strand: Direct

Name: thiD [C]

Synonym: MMP1639

Alternate gene names: 45359202

Gene position: 1580669-1581871 (Clockwise)

Preceding gene: 45359201

Following gene: 45359203

Centisome position: 95.16

GC content: 25.77

Gene sequence:

>1203_bases
ATGAATATCTTGACAGTTGGAGGTTTTGATCCAACAGGCGGTGCAGGAATCGTTGCAGATGTAAAAACAATAAAAGAGCT
TCAAAATAATCCCCTTTCAATAATTACTTCGATTATCCCTCAAAACAATAATAAAGTTTTTTTAAAAAAAGATCTATCAA
AAGAAGAAATAAAAGCACAGTTTGATGCAATTTTTGAAGATTTTAATGTAAATTGGGTTAAAACAGGAGTTTTAACCACT
GATTCAATTGATATTCTTTTAGAATTTAAAAAAAAGTATGAATTTAACATAATTTGTGATCCTGTTTTAAAATCAACCAC
CGATTTCGAATTTTCTGATGATTTTTTAAATAAAAAGTATTTCGAATTTTTTAAAGAGTGTTTTTTGATAACTCCAAATT
TAAAAGAATTTGAAATTATTAAAAAAATGTTTGAAACGTCCAAATCCAATTTAAATGATATTAATGTACTTGTAACCGGA
AAAACAGATGTTTTAAAAATTGATAATAAAAATATCGAAATAACTGGAAAATATGTGGAAAAAGAAGTCCATGGAACTGG
TTGCACATATTCTTCTGCGATTACCTGTTTTTTATCGAATGGAATGACTGTTAAAGATTCAATAGTATCTGCAAAAGAAT
TTGTGCTTGGATCAGTAATCTATGCAAAAAAAACAAAATTTGGATACAATTCAAATCCAACAAGTATTGATAAAGAATCT
GTTGAAAAAAATTTAGCCTATGCATTTAATTTAATTAAAAATATAGACATTTCGTTAGCTGATCCTGATTTTATAATTTC
AGAAAGTATTCTTTTACCAGAAACGTATTTTGATATTGCAACAGTCAAAAATAACGGAGATATCAACTTTGGAATTTCAA
ATACTAATTCAGATTTATTATTAAAATTAAAAGAATTTAATCCAAAAATAAGATCCTGTATTTCTTTAAAAAGTGATGAA
TCCATTAATAGTAAATTAGAAAACTCAGAACTTTCAATATGTCCGATGAAACTTTTAAATGACCCTATTTCGTCATTGAA
AGAAGTATCTCTTCAAAATAATTTTGGAACCGTTTTTTATGAAACTTATCCTGATATTTTATATTTTGAAGAAAAGAAAC
CCATTTTAATAATTCTCGGGGATAATTCCCTAGAAATTGTTAAAAAGCTTGAAAAGATTGAAAAACTGATTAAAAATAGA
TAA

Upstream 100 bases:

>100_bases
AATGGATGGATTTATACTAAATTCAATAAATAGCGACTGTGTTTCAGAAGTTCCAGAAATAAAAAGAGAATACACACCAA
TTTTATTTTAGGTGGTTTTT

Downstream 100 bases:

>100_bases
AATCAAAAAATCTACATATATTACTTGAATTTTTGTGTTATTTTAAAAAAGGAAGATTTAAATAATATAAATTCAATTAT
GCTTTTAGTATTTTTATCGA

Product: phosphomethylpyrimidine kinase

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNILTVGGFDPTGGAGIVADVKTIKELQNNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQFDAIFEDFNVNWVKTGVLTT
DSIDILLEFKKKYEFNIICDPVLKSTTDFEFSDDFLNKKYFEFFKECFLITPNLKEFEIIKKMFETSKSNLNDINVLVTG
KTDVLKIDNKNIEITGKYVEKEVHGTGCTYSSAITCFLSNGMTVKDSIVSAKEFVLGSVIYAKKTKFGYNSNPTSIDKES
VEKNLAYAFNLIKNIDISLADPDFIISESILLPETYFDIATVKNNGDINFGISNTNSDLLLKLKEFNPKIRSCISLKSDE
SINSKLENSELSICPMKLLNDPISSLKEVSLQNNFGTVFYETYPDILYFEEKKPILIILGDNSLEIVKKLEKIEKLIKNR

Sequences:

>Translated_400_residues
MNILTVGGFDPTGGAGIVADVKTIKELQNNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQFDAIFEDFNVNWVKTGVLTT
DSIDILLEFKKKYEFNIICDPVLKSTTDFEFSDDFLNKKYFEFFKECFLITPNLKEFEIIKKMFETSKSNLNDINVLVTG
KTDVLKIDNKNIEITGKYVEKEVHGTGCTYSSAITCFLSNGMTVKDSIVSAKEFVLGSVIYAKKTKFGYNSNPTSIDKES
VEKNLAYAFNLIKNIDISLADPDFIISESILLPETYFDIATVKNNGDINFGISNTNSDLLLKLKEFNPKIRSCISLKSDE
SINSKLENSELSICPMKLLNDPISSLKEVSLQNNFGTVFYETYPDILYFEEKKPILIILGDNSLEIVKKLEKIEKLIKNR
>Mature_400_residues
MNILTVGGFDPTGGAGIVADVKTIKELQNNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQFDAIFEDFNVNWVKTGVLTT
DSIDILLEFKKKYEFNIICDPVLKSTTDFEFSDDFLNKKYFEFFKECFLITPNLKEFEIIKKMFETSKSNLNDINVLVTG
KTDVLKIDNKNIEITGKYVEKEVHGTGCTYSSAITCFLSNGMTVKDSIVSAKEFVLGSVIYAKKTKFGYNSNPTSIDKES
VEKNLAYAFNLIKNIDISLADPDFIISESILLPETYFDIATVKNNGDINFGISNTNSDLLLKLKEFNPKIRSCISLKSDE
SINSKLENSELSICPMKLLNDPISSLKEVSLQNNFGTVFYETYPDILYFEEKKPILIILGDNSLEIVKKLEKIEKLIKNR

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=27.843137254902, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325378, Length=264, Percent_Identity=27.6515151515151, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324997, Length=250, Percent_Identity=30.4, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=256, Percent_Identity=29.296875, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7

Molecular weight: Translated: 45193; Mature: 45193

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILTVGGFDPTGGAGIVADVKTIKELQNNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQ
CCEEEECCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHH
FDAIFEDFNVNWVKTGVLTTDSIDILLEFKKKYEFNIICDPVLKSTTDFEFSDDFLNKKY
HHHHHHCCCCEEEEECEEEECCHHHEEEEHHCCCEEEEECHHHCCCCCCCCCHHHHHHHH
FEFFKECFLITPNLKEFEIIKKMFETSKSNLNDINVLVTGKTDVLKIDNKNIEITGKYVE
HHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCEEEEEHHHH
KEVHGTGCTYSSAITCFLSNGMTVKDSIVSAKEFVLGSVIYAKKTKFGYNSNPTSIDKES
EECCCCCCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHH
VEKNLAYAFNLIKNIDISLADPDFIISESILLPETYFDIATVKNNGDINFGISNTNSDLL
HHHHHHHHHHHHHCCCEEECCCCEEEECCEECCCCCEEEEEEECCCCEEEEECCCCCEEE
LKLKEFNPKIRSCISLKSDESINSKLENSELSICPMKLLNDPISSLKEVSLQNNFGTVFY
EEEHHCCHHHHHHHCCCCCCCHHHHCCCCCCEECHHHHHCCHHHHHHHHHHCCCCCEEEE
ETYPDILYFEEKKPILIILGDNSLEIVKKLEKIEKLIKNR
ECCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILTVGGFDPTGGAGIVADVKTIKELQNNPLSIITSIIPQNNNKVFLKKDLSKEEIKAQ
CCEEEECCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHH
FDAIFEDFNVNWVKTGVLTTDSIDILLEFKKKYEFNIICDPVLKSTTDFEFSDDFLNKKY
HHHHHHCCCCEEEEECEEEECCHHHEEEEHHCCCEEEEECHHHCCCCCCCCCHHHHHHHH
FEFFKECFLITPNLKEFEIIKKMFETSKSNLNDINVLVTGKTDVLKIDNKNIEITGKYVE
HHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCEEEEEHHHH
KEVHGTGCTYSSAITCFLSNGMTVKDSIVSAKEFVLGSVIYAKKTKFGYNSNPTSIDKES
EECCCCCCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHH
VEKNLAYAFNLIKNIDISLADPDFIISESILLPETYFDIATVKNNGDINFGISNTNSDLL
HHHHHHHHHHHHHCCCEEECCCCEEEECCEECCCCCEEEEEEECCCCEEEEECCCCCEEE
LKLKEFNPKIRSCISLKSDESINSKLENSELSICPMKLLNDPISSLKEVSLQNNFGTVFY
EEEHHCCHHHHHHHCCCCCCCHHHHCCCCCCEECHHHHHCCHHHHHHHHHHCCCCCEEEE
ETYPDILYFEEKKPILIILGDNSLEIVKKLEKIEKLIKNR
ECCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]