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The map label for this gene is endA

Identifier: 45358695

GI number: 45358695

Start: 1122281

End: 1122814

Strand: Direct

Name: endA

Synonym: MMP1132

Alternate gene names: 45358695

Gene position: 1122281-1122814 (Clockwise)

Preceding gene: 45358690

Following gene: 45358696

Centisome position: 67.56

GC content: 34.46

Gene sequence:

>534_bases
ATGATGGCGAAACCTAAAAAGACGATACCGGCAAAACTCTCGGATGAGAGAATTGTAATTTACGATAAAGATGGAATATC
TCGTTTAAACGAAAAAAGATACGGAGAACTTCACGAAAACTTTCTTTCACTTTCATTTGTGGAAGGGCTCTACCTTGTAT
CAAAAAACTGGATATCCTTGAGAGATAAAAATAAAAAACTGCTCTCTTTTGAAGAATTATTCGATGTTGCACAGAATATC
GACAGAAAGTTGTGTATTCGATATCTTGCATACAAAGACCTCAGAAATCGTGGATATACTGTTCGAACTGGTTTAAAATA
CGGTTCTGATTTTAGACTTTACGAAAGAAGTAATATTGATGAGATTCACTCGAGATACCTTGTAAAAGTTTTTTCAGAAG
AAATTCCTTGCGAAATTTCCGAAATTACAGGATTTGTCAGGGTTGCACATTCTGTTAGAAAAGAATTAATTATTGCAATA
GTTGATGCTGACGGGAGCGTCGTTTACTACAACATGGGATATTTAAAGCTTTAA

Upstream 100 bases:

>100_bases
AATTATATTGAATATTTTTAAAAACTATATATAGTTTTCTCTATTATATATTAGATACATGTTTTTTATTCTGATATTTT
TATTTTTAATTTGAGGAAAT

Downstream 100 bases:

>100_bases
TTATTGGAGGAATCAATATGGCACACATAATTACGCCTGAAGGAGAAAAAAAACTACTTTCTGATATTTTAATGGCATTT
GGTGTTAAGGAAGAACATGC

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISLRDKNKKLLSFEELFDVAQNI
DRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAI
VDADGSVVYYNMGYLKL

Sequences:

>Translated_177_residues
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISLRDKNKKLLSFEELFDVAQNI
DRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAI
VDADGSVVYYNMGYLKL
>Mature_177_residues
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISLRDKNKKLLSFEELFDVAQNI
DRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNIDEIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAI
VDADGSVVYYNMGYLKL

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_METMP (Q6LY59)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_988252.1
- ProteinModelPortal:   Q6LY59
- SMR:   Q6LY59
- GeneID:   2761238
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP1132
- NMPDR:   fig|267377.1.peg.1132
- HOGENOM:   HBG297289
- OMA:   DFRLYER
- ProtClustDB:   PRK09297
- BioCyc:   MMAR267377:MMP1132-MONOMER
- BRENDA:   3.1.27.9
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 20731; Mature: 20731

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: ACT_SITE 114-114 ACT_SITE 123-123 ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISL
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCEEEE
RDKNKKLLSFEELFDVAQNIDRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNID
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHH
EIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAIVDADGSVVYYNMGYLKL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCEEEC
>Mature Secondary Structure
MMAKPKKTIPAKLSDERIVIYDKDGISRLNEKRYGELHENFLSLSFVEGLYLVSKNWISL
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCEEEE
RDKNKKLLSFEELFDVAQNIDRKLCIRYLAYKDLRNRGYTVRTGLKYGSDFRLYERSNID
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHH
EIHSRYLVKVFSEEIPCEISEITGFVRVAHSVRKELIIAIVDADGSVVYYNMGYLKL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA